1M Tris-Cl [PDF]

1) Mix 121.1 g of Tris base with 700 ml of ddH2O by stirring. 2) Add HCl in the following volumes to reach the desired p
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Idea Transcript

1 M Tris-Cl (pH 7.4 to pH 8.0) - 1 L Reagents needed:

121.1 g Tris base 700 ml ddH2O 70 ml Concentrated HCl (37.2% - 12.1M) ** NOTE: Many electronic pH Meters are unable to accurately determine the pH of concentrated Tris solutions. Be sure to use an appropriate probe. ** Directions: 1) Mix 121.1 g of Tris base with 700 ml of ddH 2O by stirring. 2) Add HCl in the following volumes to reach the desired pH: pH 7.4 = 70 ml of HCl; pH 7.6 = 60 ml of HCl; pH 8.0 = 42 ml of HCl 3) Fine adjust to the desired pH (7.4 - 8.0) with concentrated HCl. 4) Add ddH 2O until final volume is 1 L. 5) Autoclave to sterilize. Alternatively - use this Henderson-Hasselbalch calculator to determine the volume of HCl needed © 2007 Joseph T.E. Roland www.Cytographica.com

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