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A one-step process for the production of single-cell protein and amyloglucosidase Applied Microbiology and Biotechnology February 1989, Volume 30, Issue 2, pp 135–140 | Cite as Endang Sukara (1) Horst W. Doelle (1) 1. Department of Microbiology, MIRCEN-Biotechnology, University of Queensland, St. Lucia-Brisbane, Australia Biotechnology Received: 19 May 1988 Accepted: 02 September 1988 57 Downloads 3 Citations
Summary A new Rhizopus species was isolated from traditional Indonesian food, tempeh. The newly isolated species was similar in its morphological characteristics to Rhizopus oligosporus UQM 145F, but grew faster on potato-dextrose agar as well as in submerged culture. The new isolate was found to convert ground cassava tuber directly into single cell protein without pretreatment due to its high amyloglucosidase formation. From 100 g ground tuber, a dry biomass of 33.75 g containing 26.48% true protein together with 60 ml of highly active amyloglucosidase (282 units) was obtained in 12 h. The amyloglucosidase was recovered by ultrafiltration, releasing 26.226 millimol glucose/l/min from soluble starch. The crude enzyme exhibited a pH optimum between 4.6 and 5.0, a temperature optimum between 55 and 60° C and an apparent Km of 3.125 g/l. High substrate concentrations and ammonium sulphate are inhibitory to the enzyme.
Keywords Enzyme Biomass Starch Cassava Ammonium Sulphate This is a preview of subscription content, log in to check access
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About this article Cite this article as: Sukara, E. & Doelle, H.W. Appl Microbiol Biotechnol (1989) 30: 135. https://doi.org/10.1007/BF00264000 DOI (Digital Object Identifier) https://doi.org/10.1007/BF00264000 Publisher Name Springer-Verlag Print ISSN 0175-7598 Online ISSN 1432-0614 About this journal Reprints and Permissions
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