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diferentes espécies de bactérias emplacas CPS e SBA, OS resultados mostram que bacilos gram-negativos sao OS mais prev

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J. Health Biol Sci. 2016; 4(3):193-197

doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016

ORIGINAL ARTICLE

Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus Caracterização da microbiota da pele e cavidade oral de Oreochromis niloticus Edmar Maciel Lima Júnior1, Tereza de Jesus Pinheiro Gomes Bandeira2, Marcelo José Borges de Miranda3 , Guilherme Emilio Ferreira4, Ezequiel Aguiar Parente4, Nelson Sarto Piccolo5, Manoel Odorico de Moraes Filho6 1. Discente do Programa de Pós-graduação em Farmacologia da Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brasil. 2. Docente do Curso de Medicina do Centro Universitário Christus (UNICHRISTUS), Fortaleza, Ceará, Brasil. 3. Coordenador do Hospital São Marcos, Recife, Pernambuco, Brasil. 4. Discente do Curso de Medicina do Centro Universitário Christus (UNICHRISTUS), Fortaleza, Ceará, Brasil. 5. Chefe do Serviço de Cirurgia Plástica de Goiânia, Goiás, Brasil. 6. Docente do Programa de Pós-graduação em Farmacologia da Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brasil.

„•–”ƒ…– –”‘†—…–‹‘ǣ ‹•Šƒ”‡—•—ƒŽŽ›‡š’‘•‡†–‘Š‹‰Š‡”‹…”‘„‹ƒŽŽ‘ƒ†•–ŠƒŽƒ†‘”ƒ‹”ƒ‹ƒŽ•ǤŠ‡‹…”‘„‹‘–ƒ‘ˆϐ‹•Š‘•–Ž›…‘•‹•–•‘ˆPseudomonas spp., Aeromonas spp., Shewanella putrefasciens, Acinetobacter spp. and Moraxella spp. „Œ‡…–‹˜‡ǣ to analyze the oral cavity, and skin tissue microbiota on the Nile tilapia (Oreochromis niloticusȌǡƒϐ‹•Š•’‡…‹‡•”ƒ‹•‡†…‘‡”…‹ƒŽŽ›‹”ƒœ‹ŽǤMethods: Samples were collected from the oral cavity and skin of 20 Nile tilapia specimens (Oreochromis niloticus), each weighing approximately 1,000 grams. The samples were cultures for quantitative analysis on sheep blood agar (SBA) and chromID™ CPS® agar (CPS). ‡•—Ž–•ǣŽ‡˜‡†‹ˆˆ‡”‡–„ƒ…–‡”‹ƒŽ•’‡…‹‡•™‡”‡‹†‡–‹ϐ‹‡†‘ƒ†’Žƒ–‡•Ǥ ”ƒǦ‡‰ƒ–‹˜‡•’‡…‹‡•™‡”‡ the most prevalent, while gram-positive Globicatella spp, Streptococcus spp and Enterococcus faecalis were also found. Pseudomonas aeruginosa species were isolated from all samples. Gram-positive Enterococcus faecalis was found in 70 and 60% of the skin and oral samples, respectively. ‘…Ž—•‹‘: For all samples studied, the microbial load was less than 100,000 colony-forming units - CFU/g of tissue. This value is a cutoff standardized for the American ‘…‹‡–›‘ˆ‹…”‘„‹‘Ž‘‰›–‘†‹ˆˆ‡”‡–‹ƒ–‡–Š‡…ƒ—•ƒŽƒ‰‡–ˆ”‘–Š‡…‘Ž‘‹œ‡”•Ǥ Ž‹‰Š–‘ˆ–Š‹•”‡•—Ž–ƒ†…‘•‹†‡”‹‰–Š‡ƒ„•‡…‡‘ˆ‹ˆ‡…–‹‘—••‹‰•‹–Š‡ϐ‹•Š •ƒ’Ž‡•ǡ™‡…‘…Ž—†‡–Šƒ––Š‡ ˜ƒŽ—‡•ˆ‘—†‹–Š‹••–—†›”‡ϐŽ‡…–ƒ‘”ƒŽǡ‘Ǧ‹ˆ‡…–‹‘—•…‘Ž‘‹œƒ–‹‘Ȁ‹…”‘„‹‘–ƒǤ Keywords: Microbiota. Nile tilapia. Oreochromis niloticus. Fish Farming in Brazil.

Resumo Introduçãoǣ•’‡‹š‡•• ‘‘”ƒŽ‡–‡‡š’‘•–‘•ƒ…ƒ”‰ƒ•‹…”‘„‹ƒƒ•ƒ‹•‡Ž‡˜ƒ†ƒ•†‘“—‡‘•ƒ‹ƒ‹•‡–‡””ƒ‘—ƒ”Ǥ’‡”ϐ‹Ž†ƒ‹…”‘„‹‘–ƒ‡’‡‹š‡• compreende principalmente Pseudomonas spp., Aeromonas spp., Shewanella putrefasciens, Acinetobacter spp., e Moraxella spp. „Œ‡–‹˜‘ǣ analisar a microbiota da cavidade oral e do tecido da pele no Tilápia do Nilo (Oreochromis niloticus), comercialmente criado no Brasil. Métodos: Vinte espécimes de Tilápia do Nilo (Oreochromis niloticus), cada uma pesando cerca de 1.000 gramas, foram submetidas a coleta de amostras da cavidade oral e da pele. •–ƒ•ƒ‘•–”ƒ•ˆ‘”ƒ…—Ž–‹˜ƒ†ƒ•“—ƒ–‹–ƒ–‹˜ƒ‡–‡‡ž‰ƒ”•ƒ‰—‡†‡…ƒ”‡‹”‘ȋȌ‡…Š”‘ ̺̺ƒ‰ƒ”ȋȌǤ‡•—Ž–ƒ†‘•ǣ ‘”ƒ‹†‡–‹ϐ‹…ƒ†ƒ•ͳͳ diferentes espécies de bactérias em placas CPS e SBA. Os resultados mostram que bacilos gram-negativos são os mais prevalentes. Cocos gram-positivos como Globicatella spp, Streptococcus spp e Enterococcus faecalis também foram encontrados. Espécies de Pseudomonas aeruginosa foram isoladas a partir de todas as amostras. Enterococcus faecalis foi encontrado em 70 e 60% das amostras de pele e por via oral, respectivamente. Conclusão: Os resultados deste estudo mostram, para todas as amostras estudadas, uma carga de CFU de menos de 100.000 unidades formadoras de colonias - UFC / g de tecido. Este valor ±—…—–‘ˆˆ’ƒ†”‘‹œƒ†‘’‡Žƒ‘…‹‡†ƒ†‡‡”‹…ƒƒ†‡‹…”‘„‹‘Ž‘‰‹ƒǡƒϐ‹†‡†‹ˆ‡”‡…‹ƒ”‘ƒ‰‡–‡…ƒ—•ƒŽ†‘•…‘Ž‘‹œƒ†‘”‡•Ǥ‹ƒ–‡†‡•–‡•”‡•—Ž–ƒ†‘•‡ considerando a ausência de sinais infecciosos nas amostras de peixes, conclui-se que os valores CFU’s encontrados neste estudo consistem em colonização/ microbiota. ƒŽƒ˜”ƒ•Ǧ…Šƒ˜‡: Microbiota. Tilápia do Nilo. Oreochromis niloticus. Cultivo de peixe no Brasil.

INTRODUCTION O Fish farming originated 2,500 years ago in China and it was introduced in Brazil in the 1930s. The country has several ĨĂǀŽƌĂďůĞ ĐŽŶĚŝƟŽŶƐ ĨŽƌ ůĂƌŐĞͲƐĐĂůĞ ĮƐŚ ƉƌŽĚƵĐƟŽŶ͕ ƐƵĐŚ ĂƐ ůĂƌŐĞ ƚĞƌƌŝƚŽƌŝĂů ĂƌĞĂ͕ ƚƌŽƉŝĐĂů ĐůŝŵĂƚĞ͕ ĂŶĚ ĞdžƚĞŶƐŝǀĞ ƌŝǀĞƌ basins1,2. Fish farming in Brazil has been increasing in recent years. In ϮϬϭϬ͕ ƚŚĞ ĐŽƵŶƚƌLJ ǁĂƐ ƌĞƐƉŽŶƐŝďůĞ ĨŽƌ ϴϮ͘ϯй ŽĨ ĨĂƌŵĞĚ ĮƐŚ ƉƌŽĚƵĐƟŽŶ ŝŶ ^ŽƵƚŚ ŵĞƌŝĐĂ͘ ŽŵŵĞƌĐŝĂů ĨĂƌŵŝŶŐ ŽĨ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐͿ ŝŶĐƌĞĂƐĞĚ ϭϬϱй ďĞƚǁĞĞŶ ϮϬϬϯ ĂŶĚ ϮϬϬϵ͕ ĂŶĚ ŝƚ ŝƐ ĐƵƌƌĞŶƚůLJ ĐŽŶƐŝĚĞƌĞĚ ƚŚĞ ƐƉĞĐŝĞƐ ǁŝƚŚ ƚŚĞ ůĂƌŐĞƐƚĐŽŵŵĞƌĐŝĂůƉƌŽĚƵĐƟŽŶŝŶƌĂnjŝů3.

ĐĐŽƌĚŝŶŐ ƚŽ ƉƌĞǀŝŽƵƐůLJ ƉƵďůŝƐŚĞĚ ĚĂƚĂ ƚŚĞ ŵŝĐƌŽďŝŽƚĂ ŝŶ ĮƐŚ ĨĂƌŵŝŶŐ ŝƐ ŵĂŝŶůLJ ĐŽŵƉŽƐĞĚ ŽĨ ŐƌĂŵͲŶĞŐĂƟǀĞ ďĂĐƚĞƌŝĂ4,5,6. dŚĞLJ ĂůƐŽ ĞǀŝĚĞŶĐĞ ƚŚĂƚ ŵŝĐƌŽďŝŽƚĂ ĚŝǀĞƌƐŝƚLJ ŝŶ ĚŝīĞƌĞŶƚ EŝůĞ ƟůĂƉŝĂ ĨĂƌŵŝŶŐ ƐLJƐƚĞŵƐ͕ ƚŚĞƌĞ ŚĂǀĞ ďĞĞŶ ĨŽƵŶĚ ƐƉĞĐŝĞƐ ĨƌŽŵ ƚŚĞ ĨŽůůŽǁŝŶŐ ďĂĐƚĞƌŝĂů ĨĂŵŝůŝĞƐ͗ ŶƚĞƌŽďĂĐƚĞƌŝĂĐĞĂĞ ;ϰϱ ƐĂŵƉůĞƐͿ͕ DŝĐƌŽĐŽĐĐĂĐĞĂĞ ;ϴϳͿ͕ WƐĞƵĚŽŵŽŶĂĚĂĐĞĂĞ ;ϲϱͿ ĂŶĚ VibrioŶĂĐĞĂĞ;ϭϭϱͿϳ͕ϴ. dŚĞ ƐŝŐŶŝĮĐĂŶƚ ŝŶĐƌĞĂƐĞ ŝŶ ĂĐƟǀŝƟĞƐ ƌĞůĂƚĞĚ ƚŽ ƚŚĞ ĐƵůƟǀĂƟŽŶ ŽĨ ĮƐŚ ŽďƐĞƌǀĞĚ ŝŶ ƌĂnjŝů͕ ĞƐƉĞĐŝĂůůLJ ƚŚŽƐĞ ŚŽƐƚƐ ǁŝƚŚ ƉŽƚĞŶƟĂů ĨŽƌ ĐƵůƟǀĂƟŽŶ ĂŶĚ ŵĂƌŬĞƟŶŐ͕ ŚĂƐ ĐŽŶƐŝĚĞƌĂďůLJ enhanced the relevance of the studies carried out in order to

Corresponding Author: Tereza de Jesus Pinheiro Gomes Bandeira. Rua João Adolfo Gurgel, 133, Cocó. CEP: 60.190-060. Fortaleza,CE-Brazil. Email: [email protected] ‘ϐŽ‹…–‘ˆ‹–‡”‡•–ǣŠ‡ƒ—–Š‘”•†‡…Žƒ”‡‘…‘ϐŽ‹…–‘ˆ‹–‡”‡•–Ǥ Received: 2016 Jun 21; Revised: 2016 Jul 27, 2016 Ago 30; Accepted: 2016 Set 1

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Microbiota of the skin and oral cavity of Oreochromis niloticus

ĚĞƚĞƌŵŝŶĞ ĐŽŶƚĂŵŝŶĂƟŽŶ ŽĨ ƚŚĞƐĞ ĂŶŝŵĂůƐ ǁŝƚŚ ƉĂƚŚŽŐĞŶŝĐ microorganisms9͘ dŚƵƐ͕ ŝŶ ŝŶƚĞŶƐŝǀĞ ĮƐŚ ƉƌŽĚƵĐƟŽŶ ƐLJƐƚĞŵƐ͕ ŚĞĂůƚŚŝƐŽŶĞŽĨƚŚĞŵŽƐƚŝŵƉŽƌƚĂŶƚĂƐƉĞĐƚƐĨŽƌƚŚĞĐŽŵŵĞƌĐŝĂů ďƌĞĞĚŝŶŐŽĨĂŶLJŬŝŶĚ1.

The CFU counts were analyzed according to Isemberg and 'ĂƌĐŝĂ;ϮϬϭϬͿŝŶƌĞůĂƟŽŶƚŽƚŚĞƌĞĨĞƌĞŶĐĞǀĂůƵĞƐŽĨƋƵĂŶƟƚĂƟǀĞ ĐƵůƚƵƌĞƐ ŽĨ ǁŽƵŶĚ ƟƐƐƵĞƐ͘ dŚŝƐ ƉĂƌĂŵĞƚĞƌ ĚĞƚĞƌŵŝŶĞƐ ƚŚĂƚ ĨŽƌ ƐŝŐŶŝĮĐĂŶƚ ĐůŝŶŝĐĂů ŝƐŽůĂƚĞƐ ;ƉĂƚŚŽŐĞŶŝĐ ŽƌŐĂŶŝƐŵƐͿ͕ ƚŚĞ &h ǀĂůƵĞƐ ƐŚŽƵůĚ ďĞ ŵŽƌĞ ƚŚĂŶ ϭϬϱ &hͬŐ ŽĨ ƟƐƐƵĞ͘ ,ĞŶĐĞ͕ dŚĞ ƐƵĐĐĞƐƐ ŽĨ ĮƐŚ ĨĂƌŵŝŶŐ ĞŶƚĞƌƉƌŝƐĞƐ ĚĞƉĞŶĚƐ ŽŶ ŵĂŶLJ &h ĐŽƵŶƚƐ ďĞůŽǁ ϭϬϬ͕ϬϬϬ &hͬŐ ŽĨ ƟƐƐƵĞ ǁĞƌĞ ĐŽŶƐŝĚĞƌĞĚ ĨĂĐƚŽƌƐ͕ĂŶĚŽŶĞŽĨƚŚĞŵŽƐƚŝŵƉŽƌƚĂŶƚĐŽŶĐĞƌŶƐŝƐƚŚĞŚĞĂůƚŚ ƐƵŐŐĞƐƟǀĞŽĨďĂĐƚĞƌŝĂůĐŽůŽŶŝnjĂƟŽŶ12,13. ĐŽŶĚŝƟŽŶŽĨƚŚĞĂŶŝŵĂůƐďĞŝŶŐĐƵůƟǀĂƚĞĚ4. KƌĂů ŵƵĐƵƐ ǁĂƐ ƐĂŵƉůĞĚ ĨƌŽŵ ϮϬ ĮƐŚ ƌĂŶĚŽŵůLJ ĐŚŽƐĞŶ ĨƌŽŵ /Ŷ ĂĚĚŝƟŽŶ͕ ƚŚĞ ƐŬŝŶ ŽĨ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ) is ƚŚĞƚĂŶŬƉŽƉƵůĂƟŽŶ͘DƵĐƵƐǁĂƐĐŽůůĞĐƚĞĚĨƌŽŵƚŚĞƐƵƌĨĂĐĞŽĨ ďĞŝŶŐƐƚƵĚŝĞĚďLJƌĞƐĞĂƌĐŚĞƌƐŽĨŽƵƌǁŽƌŬŝŶŐŐƌŽƵƉǁŝƚŚƚŚĞĂŝŵ the oral cavity region using sterile swabs, transferred to a tube ŽĨƵƐŝŶŐƚŚĞŵĂƐĂŶŽĐĐůƵƐŝǀĞďŝŽůŽŐŝĐĂůŐƌĂŌŝŶƚŚĞƚƌĞĂƚŵĞŶƚŽĨ ĐŽŶƚĂŝŶŝŶŐ ƐĂůŝŶĞ ĂŶĚ ŝŵŵĞĚŝĂƚĞůLJ ƐƉƌĞĂĚ ƉůĂƚĞĚ͘ dŚĞ ƐǁĂď ďƵƌŶƌĂƚƐ;ZĂƩƵƐŶŽƌǀĞŐŝĐƵƐͿtŝƐƚĂƌ͕ǁŝƚŚƚŚĞŝŶƚĞŶƟŽŶŽĨƵƐĞŝŶ ǁĂƐĮƌŵůLJƌŽůůĞĚŽǀĞƌƚŚĞĂŐĂƌƉůĂƚĞƐƵƌĨĂĐĞĂŶĚǁĂƐĐĂƌĞĨƵůůLJ ƉĂƟĞŶƚƐǁŝƚŚůĂƌŐĞĂƌĞĂƐŽĨďƵƌŶƐ;ƵŶƉƵďůŝƐŚĞĚĚĂƚĂͿ͘ ƐƚƌĞĂŬĞĚŝŶƚŚĞƉůĂƚĞĐĞŶƚĞƌĂŶĚƐƉƌĞĂĚŽŶƚŚĞĨŽƵƌƋƵĂĚƌĂŶƚƐ ƵƐŝŶŐĂƉůĂƟŶƵŵůŽŽƉ͘tŚĞŶƚŚĞƐǁĂďƐĂŵƉůĞƐǁĞƌĞĐŽůůĞĐƚĞĚ͕ dŚƵƐ͕ ŵŽŶŝƚŽƌŝŶŐ ĮƐŚ ŚĞĂůƚŚ ŝƐ ĞƐƐĞŶƟĂů ƚŽ ĞŶƐƵƌĞ ƚŚĞ ƚŚĞƌĞƐƵůƚǁĂƐƐĞŵŝͲƋƵĂŶƟƚĂƟǀĞ͕ŝ͘Ğ͕͘&hͬƐǁĂď͘ ƉƌŽĚƵĐƟŽŶ ŽĨ ŚĞĂůƚŚLJ ĂŶŝŵĂůƐ ĂŶĚ ƉƌĞǀĞŶƚ ůŽƐƐ ŝŶ ĂĐƟǀŝƚLJ10,11. ŽŶƐĞƋƵĞŶƚůLJ͕ ƚŚĞ ŽďũĞĐƟǀĞ ŽĨ ƚŚŝƐ ƐƚƵĚLJ ǁĂƐ ƚŽ ĂŶĂůLJnjĞ ƚŚĞ ŽůŽŶŝĞƐ ǁŝƚŚ ŵŽƌƉŚŽůŽŐŝĐĂůůLJ ĚŝƐƟŶĐƚ ĐŚĂƌĂĐƚĞƌŝƐƟĐƐ ŽŶ ^ ŽƌĂů ĐĂǀŝƚLJ͕ ƐŬŝŶ ĂŶĚ ƐƵďĐƵƚĂŶĞŽƵƐ ƟƐƐƵĞ ŵŝĐƌŽďŝŽƚĂ ŽĨ EŝůĞ ĂŶĚW^ĂŐĂƌǁĞƌĞƐĞůĞĐƚĞĚĂŶĚƌĞͲŝƐŽůĂƚĞĚ͘WƵƌĞĐŽůŽŶŝĞƐǁĞƌĞ ƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿĐŽŵŵĞƌĐŝĂůůLJƌĂŝƐĞĚŝŶŶĞƚƉĞŶƐ ƚŚĞŶŽďƚĂŝŶĞĚĨƌŽŵƐĞůĞĐƟǀĞĐƵůƚƵƌĞŵĞĚŝĂĂŶĚƚŚĞŶƐƵďŵŝƩĞĚ in Castanhão Reservoir, located in Jaguaribe, Ceara state, Brazil. ƚŽ ŝĚĞŶƟĮĐĂƟŽŶ ƚŚƌŽƵŐŚ 'ƌĂŵ ƐƚĂŝŶŝŶŐ ĂŶĚ ƚŚĞ ĂƵƚŽŵĂƚĞĚ s/dĂďWĂƐƚĞƵƌ͘dŚĞƐƚƵĚLJǁĂƐĂƉƉƌŽǀĞĚ ďLJƚŚĞƚŚŝĐƐŽŵŵŝƩĞĞŽŶŶŝŵĂů^ƚƵĚŝĞƐŽĨ&ĞĚĞƌĂůhŶŝǀĞƌƐŝƚLJ ŽĨĞĂƌĄ͕ƵŶĚĞƌŶƵŵďĞƌϰϴͬϮϬϭϲ͘ dǁĞŶƚLJ ϭϬͲŵŽŶƚŚ ŽůĚ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ) ǁĞŝŐŚŝŶŐ ĂƉƉƌŽdžŝŵĂƚĞůLJ ϭ͕ϬϬϬ ŐƌĂŵƐ ǁĞƌĞ ŽďƚĂŝŶĞĚ ĨƌŽŵ ŶĞƚ ƉĞŶƐ ŬĞƉƚŝŶ ĂƐƚĂŶŚĆŽ ZĞƐĞƌǀŽŝƌ͘ dŚĞƐĞĮƐŚ ǁĞƌĞƌĂŝƐĞĚ ĂƚĂ ĚĞŶƐŝƚLJŽĨϮϯĮƐŚͬϮŵ3. dŚĞƐĞƐƉĞĐŝŵĞŶƐǁĞƌĞƌĞƚƌŝĞǀĞĚĨƌŽŵŶĞƚƉĞŶƐĂŶĚŝŵŵĞĚŝĂƚĞůLJ ƉůĂĐĞĚŝŶĚŝǀŝĚƵĂůůLJŝŶƐƚĞƌŝůĞƉůĂƐƟĐďĂŐƐ͕ǁŚŝĐŚǁĞƌĞƐĞĂůĞĚĂŶĚ immersed in ice at 0 o͘hƉŽŶĂƌƌŝǀĂůĂƚƚŚĞůĂď͕ƚŚĞLJǁĞƌĞƉůĂĐĞĚ ŽŶƐƚĂŝŶůĞƐƐƐƚĞĞůƚĂďůĞƐǁŝƚŚƐŝŶŬƐ͕ǁŚŝĐŚŚĂĚďĞĞŶƉƌĞǀŝŽƵƐůLJ ĚŝƐŝŶĨĞĐƚĞĚǁŝƚŚϳϬйĞƚŚĂŶŽů͘ &ƌŽŵĞĂĐŚƟůĂƉŝĂ͕ƚǁŽϭ͘ϱdžϭ͘ϱĐŵƐŬŝŶĨƌĂŐŵĞŶƚƐǁĞƌĞŽďƚĂŝŶĞĚ͕ ǁŚŝĐŚ ǁĞŝŐŚĞĚ ĂƉƉƌŽdžŝŵĂƚĞůLJ Ϭ͘ϭϵ Ő͘ dŚĞƐĞ ĨƌĂŐŵĞŶƚƐ ǁĞƌĞ ƐĞĞĚĞĚďLJŝŵƉƌŝŶƟŶŐďŽƚŚƐŝĚĞƐŽŶƐŚĞĞƉďůŽŽĚĂŐĂƌ;^ͿĂŶĚ W^ĂŐĂƌ;ďŝŽDĠƌŝĞƵdžͿ͘ŶŽƚŚĞƌƐŬŝŶĨƌĂŐŵĞŶƚǁĂƐƚƌĂŶƐĨĞƌƌĞĚ ƚŽ ĂŶ ĞŵƉƚLJ ƐƚĞƌŝůĞ WĞƚƌŝ ĚŝƐŚ͕ ƚŽ ǁŚŝĐŚ ϭ ŵ> ŽĨ ƐƚĞƌŝůĞ ƐĂůŝŶĞ ƐŽůƵƟŽŶǁĂƐĂĚĚĞĚ͘hƐŝŶŐĂƐĐĂůƉĞů͕ƚŚŝƐĨƌĂŐŵĞŶƚǁĂƐĐƵƚŝŶƚŽ ƐĞǀĞƌĂůƉŝĞĐĞƐĂŶĚŵŝdžĞĚǁŝƚŚƚŚĞƐĂůŝŶĞƐŽůƵƟŽŶƵŶƟůŽďƚĂŝŶŝŶŐ ĂĚĞŶƐĞƐƵƐƉĞŶƐŝŽŶ͘ŶĂůŝƋƵŽƚŽĨϭϬϬђ>ŽĨƚŚŝƐƐƵƐƉĞŶƐŝŽŶǁĂƐ ƐƉƌĞĂĚ ŽŶ ^ ĂŶĚ W^ ĂŐĂƌ ǁŝƚŚ ŵŝĐƌŽďŝŽůŽŐŝĐĂů ůŽŽƉƐ͕ ĨŽƌ ĨƵƌƚŚĞƌĐŽƵŶƟŶŐŽĨĐŽůŽŶLJͲĨŽƌŵŝŶŐƵŶŝƚƐ;&hͿ͘dŚĞƌĞŵĂŝŶŝŶŐ ŵĂƚĞƌŝĂůǁĂƐƚŚĞŶƚƌĂŶƐĨĞƌƌĞĚƚŽĂƚĞƐƚƚƵďĞǁŝƚŚϯŵ>ŽĨďƌĂŝŶͲ ŚĞĂƌƚͲŝŶĨƵƐŝŽŶ;,/ͿŵĞĚŝƵŵĨŽƌĨƵƌƚŚĞƌĂŶĂůLJƐŝƐ͘dŚĞƐĞƉůĂƚĞƐ ĂŶĚƚƵďĞƐǁĞƌĞƚŚĞŶŝŶĐƵďĂƚĞĚĂƚϯϱǑ;нͬͲϭΣͿĨŽƌϮϰŚŽƵƌƐ͘

^ƚĂƟƐƟĐĂů ŶĂůLJƐŝƐ͗ dŚĞ ƌĞƐƵůƚƐ ŽďƚĂŝŶĞĚ ĨƌŽŵ ƚŚĞ ƐƚƵĚLJ ǁĞƌĞ ƐƵďũĞĐƚĞĚƚŽƚŚĞŶŽǀĂƐƚĂƟƐƟĐĂůĂŶĂůLJƐŝƐŽĨǀĂƌŝĂŶĐĞ;dĂďůĞϭͿ͘ Table 1͘DŝĐƌŽŽƌŐĂŶŝƐŵŝƐŽůĂƟŽŶƉƌŽƉŽƌƟŽŶŝŶϮϬŽƌĂůĂŶĚϮϬ ƐŬŝŶƐĂŵƉůĞƐĨƌŽŵEŝůĞƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿ͕ĐŽůůĞĐƚĞĚ ĨƌŽŵĮƐŚĂƌŵŶĞƚƉĞŶƐŝŶĂƐƚĂŶŚĆŽZĞƐĞƌǀŽŝƌ͕&ŽƌƚĂůĞnjĂ͕ĞĂƌĄ͕ ƌĂnjŝů͕ŝŶEŽǀĞŵďĞƌϮϬϭϱ͘ Microorganisms

Skin Samples(20) n (%)

Oral Cavity Samples (20) n (%)

ϮϬ;ϭϬϬͿ

ϮϬ;ϭϬϬͿ

Aeromonas sóbria

ϭϳ;ϴϱͿ

ϭϲ;ϴϬͿ

͘D͕͘^ĐŽĂƌŝƐ͘K͕͘WĞĚƌŽƐŽZ͕͘͘ŝƩĞŶĐŽƵƌƚE͘>͘Z͕͘EĂŬĂŵƵƌĂ͘s͕͘ hĞĚĂŶĂŬĂŵƵƌĂ d͕͘ Ğƚ Ăů͘ ĂĐƚĞƌŝĂů ŵŝĐƌŽďŝŽƚĂ ŝŶ ƚŚĞ ŐĂƐƚƌŽŝŶƚĞƐƟŶĂů ƚƌĂĐƚ ŽĨ EŝůĞ ƟůĂƉŝĂ͕ KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ ĐƵůƚƵƌĞĚ ŝŶ Ă ƐĞŵŝͲŝŶƚĞŶƐŝǀĞ ƐLJƐƚĞŵ͘ ĐƚĂ ^ĐŝĞŶƟĂƌƵŵ͘ŝŽůŽŐŝĐĂů^ĐŝĞŶĐĞƐ͘ϮϬϬϯ͖Ϯϱ;ϮͿ͗ϮϲϳͲϮϳϭ͘ ϴ͘WĂƌŬŝŶŐŬŝŶŐZ͕͘WĂůŵĂW͕͘hƐĞƌŽZ͘YƵĂŶƟƚĂƟǀĞĂŶĚƋƵĂůŝƚĂƟǀĞĂŶĂůLJƐĞƐŽĨƚŚĞ ďĂĐƚĞƌŝĂůŵŝĐƌŽďŝŽƚĂŽĨƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿĐƵůƚƵƌĞĚŝŶĞĂƌƚŚĞŶƉŽŶĚƐ ŝŶ ƚŚĞ WŚŝůŝƉƉŝŶĞƐ͘ tŽƌůĚ DŝĐƌŽďŝŽů ŝŽƚĞĐŚŶŽů͘ ϮϬϭϱ &Ğǀ͖ ϯϭ;ϮͿ͗ϮϲϱͲϮϳϱ͘ ĚŽŝ͗

J. Health Biol Sci. 2016; 4(3):193-197

ϭϵϳ

Microbiota of the skin and oral cavity of Oreochromis niloticus

ϭϬ͘ϭϬϬϳͬƐϭϭϮϳϰͲϬϭϰͲϭϳϱϴͲϭ͘WƵďDĞĚWD/͗Ϯϱϱϱϱϯϳϱ͘ ϵ͘ĂƌŽŶLJ'͘D͕͘dĂǀĂƌĞƐ'͕͘͘ƐƐŝƐ'͘͘E͕͘>ƵnjZ͘ĞĂů͘͘ EĞǁ ŚŽƐƚƐ ĂŶĚ ŐĞŶĞƟĐ ĚŝǀĞƌƐŝƚLJ ŽĨ &ůĂǀŽďĂƚĞƌŝƵŵ ĐŽůƵŵŶĂƌĞ ŝƐŽůĂƚĞĚ ĨƌŽŵ ƌĂnjŝůŝĂŶEĂƟǀĞƐƉĞĐŝĞƐĂŶĚEŝůĞƟůĂƉŝĂ͘ŝƐƋƵĂƚKƌŐĂŶ͘ϮϬϭϱEŽǀϭϳ͖ϭϭϳ;ϭͿ͗ϭͲ ϭϭ͘ĚŽŝ͗ϭϬ͘ϯϯϱϰͬĚĂŽϬϮϵϯϭ͘ ϭϬ͘ƵƐƟŶ͕͘dŚĞďĂĐƚĞƌŝĂůŵŝĐƌŽŇŽƌĂŽĨĮƐŚ͕ƌĞǀŝƐĞĚ͘^ĐŝĞŶƟĮĐtŽƌůĚ:ŽƵƌŶĂů͘ ϮϬϬϲƵŐϭϭ͘ϲ͗ϵϯϭʹϵϰϱ͘ ϭϭ͘ ,ƵŝĐĂďͲWĞĐŚ ͘'͕͘ >ĂŶĚĞƌŽƐͲ^ĄŶĐŚĞnj ͕͘ ĂƐƚĂŹĞĚĂͲŚĄǀĞnj D͘Z͕͘ >ĂŶŐŽͲZĞLJŶŽƐŽ &͕͘ >ſƉĞnjͲŽůůĂĚŽ ͘:͕͘ WůĂƚĂƐ ZŽƐĂĚŽ ͘͘ ƵƌƌĞŶƚ ƐƚĂƚĞ ŽĨ ďĂĐƚĞƌŝĂ ƉĂƚŚŽŐĞŶŝĐŝƚLJ ĂŶĚ ƚŚĞŝƌ ƌĞůĂƟŽƐŚŝƉ ǁŝƚŚ ŚŽƐƚ ĂŶĚ ĞŶǀŝƌŽŶŵĞŶƚ ŝŶ ƟůĂƉŝĂ KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ͘ ƋƵĂĐ͘ ϮϬϭϲ͖ ϳ;ϱͿ͗ϭͲϭϬ͘ ĚŽŝ͗ ϭϬ͘ϰϭϳϮͬϮϭϱϱͲ ϵϱϰϲ͘ϭϬϬϬϰϮϴ͘ ϭϮ͘ŐġŶĐŝĂEĂĐŝŽŶĂůĚĞsŝŐŝůąŶĐŝĂ^ĂŶŝƚĄƌŝĂ͘DŝĐƌŽďŝŽůŽŐŝĂĐůşŶŝĐĂƉĂƌĂŽĐŽŶƚƌŽůĞ ĚĞ ŝŶĨĞĐĕĆŽ ƌĞůĂĐŝŽŶĂĚĂ ă ĂƐƐŝƐƚġŶĐŝĂ ă ƐĂƷĚĞ͘ DſĚƵůŽ ϰ͗ ƉƌŽĐĞĚŝŵĞŶƚŽƐ ůĂďŽƌĂƚŽƌŝĂŝƐ͗ ĚĂ ƌĞƋƵŝƐŝĕĆŽ ĚŽ ĞdžĂŵĞ ă ĂŶĄůŝƐĞ ŵŝĐƌŽďŝŽůſŐŝĐĂ Ğ ůĂƵĚŽ ĮŶĂů͘ ƌĂƐşůŝĂ͗Es/^͖ϮϬϭϯ͘ϵϱƉ͘ ϭϯ͘'ĂƌĐŝĂ͕>͘^͖͘/ƐĞŶďĞƌŐ,͘͘ůŝŶŝĐĂůDŝĐƌŽďŝŽůŽŐLJWƌŽĐĞĚƵƌĞƐ,ĂŶĚŬ͘ϯƌĚ͘ ĞĚ͘tĂƐŚŝŶŐƚŽŶ͗ŵĞƌŝĐĂŶ^ŽĐŝĞƚLJĨŽƌDŝĐƌŽďŝŽůŽŐLJ͖ϮϬϭϬ͘ ϭϰ͘WŝŶĐƵƐ͕͘,͘DŝĐƌŽďŝĂůŝĚĞŶƟĮĐĂƟŽŶƵƐŝŶŐƚŚĞďŝŽDĞƌŝĞƵdžs/d͘^͖͘ ĂǀĂůĐĂŶƚĞ͕ ͘,͖͘ ĂƌǀĂůŚŽ͕ &͘͘d͖͘ sŝĞŝƌĂ͕ Z͘,͘^͘&͖͘ ^Ą D͘s͘͘

ĂŶĚ ^ŽƵƐĂ͕ K͘s͘ ƋƵĂƟĐ ŵŝĐƌŽďŝŽƚĂ ĚŝǀĞƌƐŝƚLJ ŝŶ ƚŚĞ ĐƵůƚƵƌĞ ŽĨ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐͿ ƵƐŝŶŐ ďŝŽŇŽĐƐ Žƌ ƉĞƌŝƉŚLJƚŽŶ͗ ǀŝƌƵůĞŶĐĞ ĨĂĐƚŽƌƐ ĂŶĚ ďŝŽĮůŵĨŽƌŵĂƟŽŶ͘ĐƚĂ^ĐŝĞŶƟĂƌƵŵ͘ŶŝŵĂů^ĐŝĞŶĐĞƐ͘ϮϬϭϲ:ƵůͲ^ĞƉ͖ϯϴ;ϯͿ͗ϮϯϯͲ Ϯϰϭ͘ĚŽŝ͗ŚƩƉ͗ͬͬĚdž͘ĚŽŝ͘ŽƌŐͬϭϬ͘ϰϬϮϱͬĂĐƚĂƐĐŝĂŶŝŵƐĐŝ͘ǀϯϴŝϯ͘ϯϭϵϭϬ͘ ϭϴ͘ŶƐŚĂƌLJ,͕͘ĂŬĞ^ĞŶƚĂŶŝ͕WĂƉƵĂ͕/ŶĚŽŶĞƐŝĂ͘ ^ƉƌŝŶŐĞƌƉůƵƐ͘ ϮϬϭϰ KĐƚ Ϯϰ͖ ϯ;ϲϮϳͿ͗ϭͲϭϭ͘ ĚŽŝ͗ ϭϬ͘ϭϭϴϲͬϮϭϵϯͲϭϴϬϭͲϯͲϲϮϳ͘ WƵďDĞĚWD/͗ϮϱϯϵϮϳϵϳ͘ ϭϵ͘ůƐŝŶŐŚŽƌƐƚd͘͘&ŝƌƐƚĐĂƐĞƐŽĨĂŶŝŵĂůĚŝƐĞĂƐĞƐƉƵďůŝƐŚĞĚƐŝŶĐĞϮϬϬϬ͘sĞƚY͘ ϮϬϬϯ͖Ϯϱ;ϰͿ͗ϭϲϱͲϭϲϵ͘ ĚŽŝ͗ ϭϬ͘ϭϬϴϬͬϬϭϲϱϮϭϳϲ͘ϮϬϬϯ͘ϵϲϵϱϭϲϬ͘ WƵďDĞĚ WD/͗ ϭϰϳϭϰϳϰϬ͘ ϮϬ͘sĞůĂ͘/͕͘&ĞƌŶĄŶĚĞnj͕͘>ĂƐ,ĞƌĂƐ͕͘>ĂǁƐŽŶW͕͘͘ŽŵşŶŐƵĞnj>͕͘ŽůůŝŶƐD͕͘͘ &ĞƌŶĂŶĚĞnjͲ'ĂƌĂLJnjĂďĂů :͘&͘ DĞŶŝŶŐŽĞŶĐĞƉŚĂůŝƟƐ ĂƐƐŽĐŝĂƚĞĚ ǁŝƚŚ Globicatella ƐĂŶŐƵŝŶŝƐ ŝŶĨĞĐƟŽŶ ŝŶ ůĂŵďƐ͘ : ůŝŶ DŝĐƌŽďŝŽů͘ ϮϬϬϬ EŽǀ͖ ϯϴ;ϭϭͿ͗ϰϮϱϰͲϰϮϱϱ͘ WƵďDĞĚĞŶƚƌĂůWD/͗WDϴϳϱϳϱ͘ Ϯϭ͘ŽůůŝŶƐD͕͘͘ZŽĚƌŝŐƵĞnj:͘D͕͘>ĂǁƐŽŶW͕͘͘&ĂůƐĞŶ͕͘&ŽƐƚĞƌ'͘ŚĂƌĂĐƚĞƌŝnjĂƟŽŶ ŽĨĂŶŽǀĞůŐƌĂŵͲƉŽƐŝƟǀĞ͕ĐĂƚĂůĂƐĞͲŶĞŐĂƟǀĞĐŽĐĐƵƐĨƌŽŵŚŽƌƐĞƐ͗ĚĞƐĐƌŝƉƟŽŶŽĨ ƌĞŵŽĐŽĐĐƵƐĐŽůĞŽĐŽůĂŐĞŶ͘ŶŽǀ͕͘ƐƉ͘ŶŽǀ͘/Ŷƚ:^LJƐƚĂĐƚĞƌŝŽů͘ϭϵϵϵKĐƚ͖ϰϵ;Wƚ ϰͿ͗ϭϯϴϭͲϭϯϴϱ͘ĚŽŝ͗ϭϬ͘ϭϬϵϵͬϬϬϮϬϳϳϭϯͲϰϵͲϰͲϭϯϴϭ͘WƵďDĞĚWD/͗ϭϬϱϱϱϯϭϲ͘ ϮϮ͘ ƌŝůŚĂŶƚĞ Z͘^͕͘ ĂƐƚĞůŽͲƌĂŶĐŽ ͘^͕͘ ^ŽĂƌĞƐ͕ '͕͘͘ ƐƚĞƚĞͲDĞĚƌĂŶŽ ͘:͕͘ DŽŶƚĞŝƌŽ ͘:͕͘ ŽƌĚĞŝƌŽ Z͕͘͘ Ğƚ Ăů͘ ŚĂƌĂĐƚĞƌŝnjĂƟŽŶ ŽĨ ƚŚĞ ŐĂƐƚƌŽŝŶƚĞƐƟŶĂů LJĞĂƐƚ ŵŝĐƌŽďŝŽƚĂ ŽĨ ĐŽĐŬĂƟĞůƐ ;ELJŵƉŚŝĐƵƐ ŚŽůůĂŶĚŝĐƵƐͿ͗ Ă ƉŽƚĞŶƟĂů ŚĂnjĂƌĚ ƚŽ ŚƵŵĂŶ ŚĞĂůƚŚ͘ : DĞĚ DŝĐƌŽďŝŽů͘ ϮϬϭϬ :ƵŶ͖ ϱϵ;Wƚ ϲͿ͗ ϳϭϴʹϳϮϯ͘ ĚŽŝ͗ ϭϬ͘ϭϬϵϵͬ ũŵŵ͘Ϭ͘ϬϭϳϰϮϲͲϬ͘WƵďDĞĚWD/͗ϮϬϭϱϬϯϭϴ͘ Ϯϯ͘ƌŝůŚĂŶƚĞZ͘^͕͘:ĞƐƵƐ^͘Z͘d͕͘^ŽƵnjĂ͘D͕͘͘͘dĞŝdžĞŝƌĂ͕͘͘ƌŝƚŽD͘Z͕͘ĂŶĚĞŝƌĂ ^͘W͕͘ Ğƚ Ăů͘ ĂŶĚŝĚĂ ƉĂƌĂƉƐŝůŽƐŝƐ ĐŽŵƉůĞdž ĨƌŽŵ ĂŶŝŵĂůƐ ĂŶĚ ŝƚƐ ĂŶƟĨƵŶŐĂů ƐƵƐĐĞƉƟďŝůŝƚLJ ĂŶĚ ǀŝƌƵůĞŶĐĞ ĂƩƌŝďƵƚĞƐ͘  DĞĚ DŝĐƌŽďŝŽů͘ ϮϬϭϰ EŽǀ͖ ϲϯ;Wƚ ϭϭͿ͗ ϭϱϲϴͲϭϱϳϮ͘ĚŽŝ͗ϭϬ͘ϭϬϵϵͬũŵŵ͘Ϭ͘ϬϳϲϮϭϲͲϬ͘WƵďDĞĚWD/͗ϮϱϭϵϬϳϯϲ͘ Ϯϰ͘^ŝĚƌŝŵ:͘:͕͘ĂƌǀĂůŚŽs͘>͕͘ĂƐƚĞůŽͲƌĂŶĐŽ͘^͕͘ƌŝůŚĂŶƚĞZ͘^͕͘ĂŶĚĞŝƌĂd͘:͕͘ ŽƌĚĞŝƌŽZ͕͘͘ĞƚĂů͘zĞĂƐƚŵŝĐƌŽďŝŽƚĂŽĨŶĂƚƵƌĂůĐĂǀŝƟĞƐŽĨŵĂŶĂƚĞĞƐ;dƌŝĐŚĞĐŚƵƐ inunguis and Trichechus manatus ) in Brazil and its relevance for animal health ĂŶĚŵĂŶĂŐĞŵĞŶƚŝŶĐĂƉƟǀŝƚLJ͘ĂŶ:DŝĐƌŽďŝŽů͘ϮϬϭϱKĐƚ͘ϲϭ;ϭϬͿ͗ϳϲϯͲϳϲϵ͘

‘‘…‹–ƒ”‡•–‡ƒ”–‹‰‘Ȁ ‘™–‘…‹–‡–Š‹•ƒ”–‹…Ž‡ǣ Lima EM Júnior, Bandeira TJPG, Miranda MJB, Moraes MO, Ferreira GE, Parente EA, Picollo NS. Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus. J Health Biol Sci. 2016 Abr-Jun; 4(3):193-197. J. Health Biol Sci. 2016; 4(3):193-197

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