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BE518 Optical Microscopy 1 CU (3 semester hours) Lecture: 3 hrs/week. Recitation 1 hr/wk. Christopher Fang-‐Yen, PhD, Assistant Professor of Bioengineering Math 240 or ENM 510 or equivalent, or permission of the instructor [ ] Math [ ] Sciences [ X ] Engineering [ X ] Technical Elective [ ] TBS th Hecht, Eugene (2001). Optics, 4 Edition. Addison Wesley, Course materials This course discusses the fundamental concepts of optics and microscopy, with emphasis on applications in biomedical imaging. Lectures will be supplemented by lab experiments. We will survey advanced modern techniques including confocal, multiphoton, and superresolution microscopy. Light and the electromagnetic spectrum. Geometrical optics: optical rays, refraction, reflection, lenses, mirrors, prisms, optical systems, optical design, aberrations. Physical optics: waves, diffraction, interference, resolution limits, coherence, lasers, Gaussian beams. Microscopy methods: Phase contrast, differential interference contrast, fluorescence microscopy, confocal microscopy, multiphoton microscopy, optical coherence tomography. Superresolution techniques: structured illumination, STED, PALM. The goal of this course is for students to gain an understanding of the principles of optics and the application of these principles in microscopy. Students will learn to design and construct simple optical systems. Multidisciplinary Ability – Med Problem Solving Approach – High Problem Solving Methods – High Experimentation – Med Design – Med Professional Orientation -‐-‐ Low 75% Engineering science 25% Engineering mathematics Week 1: Foundations: Maxwell’s equations. The wave equation. Light and the electromagnetic spectrum. Week 2: Foundations: Index of refraction. Reflection and transmission. Polarization. Week 3: Geometrical optics : Optical rays, lenses, mirrors, prisms Week 4: Geometrical optics: Image formation Week 5: Geometrical optics: Optical systems, telescopes, microscopes, cameras, the eye Week 6: Geometrical optics: Optical design, optical aberrations Week 7: Physical optics: Diffraction Week 8: Physical optics: interference, coherence theory Week 9: Physical optics: Fourier optics, Abbe theory Week 10: Physical optics: Resolution limits in microscopy Week 11: Microscopy methods: Phase contrast, differential interference contrast Week 12: Microscopy methods: Fluorescence microscopy, confocal microscopy Week 13: Microscopy methods: Multiphoton microscopy, optical coherence tomography Week 14: Microscopy methods: Superresolution methods: structured illumination, STED, PALM 30% Homework and lab assignments 40% Midterms 30% Final exam Christopher Fang-‐Yen / September 2012