BIO 205 Microbiology with Lab Course Package [PDF]

Apr 3, 2009 - Lab hours 3. Prerequisites. BIO 100, BIO 150, BIO 181 or the permission of the instructor. Contact person(

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BIO 205 Microbiology with Lab

(Title Change ONLY – Oct. 2013)

Course Package

Approved December 10, 2004 Effective Spring 2005 Modified April 3, 2009

COURSE INFORMATION Title

MICROBIOLOGY

Number

BIO 205

Catalog Course Description: A study of the structure, function, and classification of microorganisms and their application to industrial, agricultural, and medical problems. Credit hours

4

Prerequisites

Lecture 3 Lab hours 3 hours BIO 100, BIO 150, BIO 181 or the permission of the instructor

Contact person(s)

Don Plantz (BHC), Jeff Kingsbury (LHC), Tom McKinney (KGM)

Intended Course Outcomes Course Goals

1. Students will demonstrate an understanding of microbial growth and metabolism. 2. Students will demonstrate an understanding of the control of microorganisms 3. Students will demonstrate an understanding of the relationships between microbes and human society including microbes as disease agents, microbial use in industrial processes, and microbial use in agriculture. 4. Students will demonstrate an understanding of microbes in the natural world including microbial classification, the role of microbes in local, regional and global ecology, and in their evolved interactions with other organisms. 5. Students, in the laboratory, will demonstrate safe techniques used to manipulate microbial growth and some of the techniques used for the identification and classification of microorganisms.

Course Competencies and Objectives

Competency 1 To distinguish between the various groups of microorganisms and discuss, where appropriate, the ecological, industrial, agricultural, and medical aspects of each group. Objective 1.1 Identify specific groups and subgroups of each microbial taxon.

Objective 1.2 List and discuss characteristics of selected microorganisms that make them useful in ecological, industrial, agricultural and medical processes. Competency 2 Demonstrate an understanding of microbial metabolic processes, the importance of these in microbial activities to us and in the natural world and their use in the identification of microorganisms. Objective 2.1 Using previously gained knowledge of metabolic processes, compare microorganisms to other organisms and indicate how the microbial processes and products differ from those of most multicellular organisms. Objective 2.2 Use the unique aspects of microbial metabolism to identify selected microorganisms in the laboratory setting. Competency 3 Demonstrate an understanding of microbial grtowth patterns, the ecological factors affecting these patterns and a variety of mechanisms for controlling bacterial growth. Objective 3.1 Draw the bacterial growth curve and explain what is occuring in each stage of growth. Objective 3.2 List the ecological factors which control microbial growth and indicate how the pattern is changed as each factor changes Objective 3.3 Discuss the materials and processes commonly used by humans to control the growth of microorganisms. Competency 4 Demonstrate an understanding of bacterial genetics and recombinant DNA technology as it relates to microorganisms. Objective 4.1 Illustrate the mechanisms of genetic information transfer from one individual to another Objective 4.2 Outline the process by which genetic information is modified through recombinant DNA technology. Objective 4.3 Provide examples of genetically modified organism. Objective 4.4 List and discuss the health and environmental concerns regarding recombinant DNA technology Competency 5 Demonstrate a knowledge of microbial classification and the ability to identify selected microorganisms in the laboratory setting. Objective 5.1 Distinguish between and describe characteristics fo each of the major microbial groups, and discuss the role of those organisms in both the natural and the human world. Objective 5.2 Demonstrate laboratory techniques used to identify microorganisms and use these techinques to identify exanmples of unknown organisms. Competency 6 Discuss microbial diseases both generally and specifically and the vertebrate immune system response to disease and infection including mechanisms of pathogenicity, disease signs and symptoms, nonspecific and specific immune responses, and practical immunity. Objective 6.1 Discuss the structure of the major human organ systems and each of the major diseases of each of these systems. Objective 6.2 Describe and discuss how different organisms cause organic

damage to tissue and how this damage modifies organ system structure and function. Objective 6.3 Describe the functioning of the vertebrate immune system and detail how non-self items are defeated by this system. Objective 6.4 Students will describe immunological tests used to identify microorganisms and other medical conditions. Competency 7 Students will understand the role of microorganisms in industry and agriculture and demonstrate some of these activities in the laboratory setting Objective 7.1 Detail the use fo microorganisms in producting a variety of industrial products. Objective 7.2 Detail microorganisms use to enhance productivity in the agricultural setting. Objective 7.3 Demonstrate selected industrial/agricultural production process in the laboratory. Competency 8 Students will demonstrate an understanding of the role of microorganisms in ecological/environmental processes in natural settings and demonstrate these processes (in a limited fashion) in the laboratory setting. Objective 8.1 Discuss the role of microorganisms in biogeochemical cycles, as sources on energy in terrestrial and aquatic food webs, and their importance in natural and human originated bioremediation. Objective 8.2 Use selected laboratory tests to demonstrate the role of bacteria in maintaining environmental quality and to assess microbial activity in the environment.

Teacher’s Guide Course Materials and Equipment Textbook(s)

Software

Title Author(s) Publisher ISBN

Microbiology, An Introduction (Tenth Edition) Tortora, Funk, Case Pearson 0-321-55007-2

Title Publisher ISBN Also available as an e-book

Equipment Other

Learning Units

Learning Unit Topic 1: Introduction to Microbiology Competency: 1 Objectives: 1.1, 1,2 Activities/ Assignments: Readings-Textbook Chapters 1, 3, 4 and Lab Manual Pages 29-56. Lecture and class discussions- Introduction to Microbiology, classification of microorganisms, applied microbiology, medical microbiology. Media- Blackboard presentation- Introduction to Microbiology, and Basic Microscopy. Laboratory Exercises- Lab manual exercises 1 and 5-7 , Microscopy and Survey of Microorganisms Learning Unit Topic 2: Microbial Metabolism and Biochemistry Competency: 2 Objectives: 2.1, 2.2 Activities/ Assignments: Readings- Textbook: Chapters 2, 5, Lab manual Pages 173-216. Lecture and Class Discussion- Biochemistry and metabolism in microorganisms. Media- Blackboard presentations on Biochemistry of microorganisms and Microbial Metabolism. Laboratory Exercises: Lab manual exercises 26-34 Control of Microbial Growth Learning Unit Topic 3: Understanding Microbial Growth Competency: 3 Objectives: 3.1, 3.2, 3.3 Activities/ Assignments: Readings- Textbook: Chapter 6 and 7, Lab manual pages 57-69. Lecture and Class discussions- methods of evaluating microbial growth, important parameters governing microbial growth, methods of controlling microbial growth. Media- Blackboard presentation- Microbial Growth I and II. Laboratory Exercises: Lab manual exercise 8, Use of Aseptic Technique

Learning Unit Topic 4: Bacterial Genetics and Recombinant DNA tecnology Competency: 4, 7 Objectives: 4.1, 4.2, 4.3, 4.4, 7.1, 7.2, 7.3 Activities/ Assignments: Readings- Textbook: Chapters 8,9 Lab Manual pages 249-255. Lecture and Class discussions- Introduction genetics and the structure and function of DNA, Regulation of gene expression, concepts of mutation, recombinant DNA technology, medical and industrial applications of recombinant DNA technology in microorganisms. Media- Blackboard presentations on Genetics and Recombinant Biotechnology. Laboratory Exercises: exercise 41 use of Bergey’s manual Learning Unit Topic 5: Classification of Microbes Competency: 5 Objectives: 5.1, 5.2 Activities/ Assignments: Readings- Textbook: Chapters 10, 11, 12, 13 Lab manual pages 217-248. Lecture and Class discussions- Review of Phylogeny and taxonomy, discussion of prokaryotes, eukaryotes, protozoa, fungi, and viruses. Media- Blackboard presentations Classification I and II. Laboratory exercise- 35 morphologic study of bacterium Learning Unit Topic 6: Principles of Infectious disease, Immunology, and chemotherapy Competency: 6 Objectives: 6.1, 6.2, 6.3, 6.4 Activities/ Assignments: Reading- Textbook: Chapter 14, 16, 17, 18, 19, and 20. Lecture and Class discussions- Defining pathology, infection and disease, etiology of infectious diseases, review of infectious disease transmission, specific and non-specific host defense mechanisms, and review of common infectious diseases the major physiologic organ systems, antibiotic chemotherapy. Media- Blackboard presentations on Infectious diseases, Immunology, Antibiotics. Laboratory Exercises: Lab manual exercises 37 and 41, Identifying unknowns using Bergey’s manual Learning Unit Topic 7: The role of microorganisms in ecological proceese Competency: 8 Objectives: 8.1, 8.2 Activities/ Assignments: Readings- Textbook: Chapter 27 and Lab Manual pages 285-297. Lecture and Class discussions- Principles of symbiosis and habitat, soil and aquatic microbiology, food and disease. Media- Blackboard presentation- Environmental Microbiology. Laboratory Exercises: Lab manual exercise 47, Examination of water samples

Course Assessment Information Description of Course Assessments (Essays, multiple choice, etc.)

Essay tests, Multiple choice tests, Lab reports, Library reports

Are exams standardized in your department? Yes No Where can faculty members locate or access the exams for this course?

Are the exams required by the department? Yes NO Instructors may use test bank that accompanies the text, in consultation with other faculty members.

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