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RECOMMENDED Rapid Biuret Protein Determination Rapid biuret protein determination Aim To become familiar with the biuret method for protein determination To use the biuret method (/documents/rapid-biuret-proteinto determine the concentration… determination.html)
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A Micro Biuret Method for Protein Determination Determination of Total Protein in Cerebrospinal Fluid A MICRO BIURET METHOD FOR PROTEIN DETERMINATION DETERMINATION OF TOTAL PROTEIN JN CEREBROSPINAL (/documents/a-micro-biuret-method-for-protein-determinationFLUID BY J. GOA From the Ccntral Laboratory, Sahlgrenâs Hospital, determination-of-total-protein.html) Gothcnbicrg… Lab 9 protein estimation biuret method 1. 1Biochemistry Lab :
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Quantitative Determination of Protein by the Biuret &Lowery Reactions Quantitative Determination of Protein by the Biuret &Lowery Reactions. CLS 331 Exp.no.5. Introduction There are many methods for the (/documents/quantitative-determination-of-protein-by-thedetermination of the amount of protein…
CORRESPONDENCE. MODIFICATION OF BIURET METHOD OF PROTEIN DETERMINATION
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Determination of Total Serum Proteins By Biuret Method. Determination of Total Serum Proteins By Biuret Method CASE HSTRORY A psychiatric patient was referred to the Psychiatric Clinic. (/documents/determination-of-total-serum-proteins-by-biuretHis parents complained of his refusal to…
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Assessment of a biuret method without concentration step for total protein determination in cerebrospinal fluid ELSEVIER PII S0009-9120(97)00038-6 Clinical Biochemistry, Vol. 30, No. 4, 313-314, 1997 Copyright © 1997 The Canadian Society of (/documents/assessment-of-a-biuret-method-withoutClinical Chemists Printed in the USA.… concentration-step-for-total-protein.html)
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Biuret Test Untuk Protein Biuret Test Untuk Protein Kehadiran ikatan peptida dideteksi dengan melakukan tes kimia bernama uji biuret. Dalam tes ini sampel. (/documents/biuret-test-untuk-protein.html) Adalah pertama dipanaskan dan kemudian Sodium…
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BAB I PENDAHULUAN A. Latar belakang Protein banyak terkandung di dalam makanan yang sering dikonsumsi oleh manusia. Seperti (/documents/biokimia-protein-biuret.html) pada tempe, tahu, ikan dan lain sebagainya. Secara…
Transcript 534 A N A L Y T I C A L C H E M I S T R Y Vacuum Manipulation of Volatile Compounds. R. T. Sanderson. viii + 162 pp. John Wiley & Sons, Inc., 440 Fourth hve., Iiew York 16, S. Y., 1948. The title of this book might convey to the casual observer an uncertainty or misconception as to its contents. The sub- title, which states that it is âa laboratory manual describing the application of high vacuum technique in experimental chemistry,â is less ambiguous. The statement of the purpose of the text as set forth in the preface is far too modest. The claim that it is to provide in- formation âto enable an investigator with an average background of scientific training and experience to construct and operate a general purpose high vacuum apparatus for chemical researchâ is Fell fulfilled. But, in addition, it should prove to be a font of information for most workers in the field of high vacuum. The emphasis is on fundamentals. Certain sections are detailed and skillfully presented-for example, the chapter on the production and measurement of low temperatures. How- ever, a similar discussion of high temperature is lacking. One might also question the wisdom of omitting some details on quartz, graded seals, glass-metal seals, and instrumental methods available for measurements in high vacuum, though references are made to some of these The sections on vapor pressure and fractionation are excellent. Safety in vacuum work is wisely emphasized. A notable and very useful feature is the inclusion in the index of an extensive table of the vapor pressures of pure compounds. This book, then, fulfills the need for a compilation of the methods and equipment to be used in vacuum chemistry, and, although not exhaustive, should be most useful as a handbook on fundamentals. JOHN J. NAUGHTON Price, $3. Bibliography on Water and Sewage Analysis. B. H . Weir , Poagie E. Murray , George W . Reid, and Robert S . Ingols. viii + 215 pages. Special Report 28. State Engineering Experiment Station, Georgia Institute of Technology, Atlanta, Ga., 1948. Price, $4. According to the authors, âThis bibliography was prepared in an effort to bring together an indexed guide of the pertinent litera- ture on water and sewage analysis., .â, It appears that this effort has been successful. The bibliography, which is not annotated, contains 2560 references and is intended to be complete through January 1, 1948. Although most of the journals consulted are for the period 1920 to 1947, references from Chemical Abstracts date back as far as 1907. The great majority of references concern water analysis. An important feature is the listing of references to abstracts such as Chemical Abstracts, as well as to original papers. The bibliography includes an author index. The subject headings are divided into 36 convenient groups, beginning with acidity, alkalinity, and aluminum and ending with toxic gases, turbidity, and zinc. In addition to the consecutively numbered references throughout the book, there is a supple mental list of cross references to methods of analysis, such a biochemical, colorimetric, and volumetric. Every serious worker in the fields of water and sewage analysis will want to have this book for reference purposes. S. K. Love Analytical Division Paper Committee The Division of Analytical and Micro Chemistry, AMERICAN CHEMICAL SOCIETY, has announced the personnel of its Paper Committee: W. M. MacNevin, chairman, A. Q. Butler, J. R. Churchill, Wm. G. Batt, G. T. Wernimont, Walter J. Murphy, and L. T. Hallett. Modification of Biuret Method of Protein Determination SIR: The biuret method of Robinson and Hogden [J . Biol. Chem., 135, 707, 727 (1940)l for the determination of serum pro- teins is RTidely used for the determination of protein in tissue homogenates, enzyme preparations, and similar materials, al- though details of such an adaptation have never appeared in the literature. In dealing with such crude materials, an annoying turbidity often appears in the final color solution, making visual measurement of the color difficult and photometric measurement inaccurate. Because this difficulty seemed to occur most often in liver from starved or protein-depleted animals, the turbidity was considered probably lipide in nature. It was found that the turbidity could be removed in the following manner: The color is developed in the usual way, including 10 minutes, standing at room temperature. The tubes are then chilled on ice for 2 hours. This coagulates the fatty turbidity and permits its ready removal together with the excess copper hydroxide, by filtration through a coarse filter paper (Schleicher and Schuell No. 410 is suggested). A certain amount of the color is absorbed by the paper, as pointed out by Robinson and Hogden, but it has been found here that if a standard curve (using rabbit serum standardized by Kjeldahl determinations) is prepared from a filtered series of standards, the curve follows Beerâs law as well as does a curve of centrifuged standards, although the slopes of the two curves are appreciably different. ROBERT 5. FEINSTEIN University of Chicago Chicago, Ill. p-Aminobenzoic Acid SIR: In Table I1 of the paper entitled âp-Aminobenzoic Acid and Its Sodium Saltâ [AKAL. CHEM., 20, 919 (1949)] we reported various pH values for aqueous solutions of paminobenzoic acid with and without added equivalent amounts of sodium hydroxide. A footnote should have been added explaining that these pH val- ues represented those of concentrated stock solutions used t o pre- pare the dilute solutions for spectrophotometric analysis. These values have been questioned by Vandenbelt and Doub ( 3 ) and t o clarify the situation further x-e have rechecked our results. We have determined that the pH of the final dilutions and changes should be made as follows: pH 3 .59 t o 5 , l l 4 . 7 8 5 . 4 9 9.19 5.82 11 .58 6.86 Doub and Vandenbeltâs (1 ) observation of wave-length max- ima a t 284 millimicrons for p-aminobenzoic acid in distilled water a t pK 3.75 is correct, However, in our studies we have attempted to demonstrate a method of spectrophotometric analysis for p- aminobenzoic acid in distilled water without added buffers or acidi- fiers a t its wave-length maxima in the solvent. We have per- formed these experiments many times and have consistently ob- tained wave-length maxima for p-aminobenzoic acid in distilled water a t 266 millimicrons.
Biuret Protein Assay Biuret Protein Assay Considerations for use The principle of the biuret assay is similar to that of the Lowry, however it involves a single (/documents/biuret-protein-assay.html) incubation of 20 min. There are…
Data Absorbansi - Protein - Biuret konsentrasi 2 mg/ml lambda absorbansi (A) konsentrasi larutan standar konsentrasi 2 4 6 8 10 12 14 16 18 20 larutan sampel 1 2 3 4 0.005 (/documents/data-absorbansi-protein-biuret.html) 0.006 0.013 0.017 absorbansi 0.4…
Organometallic “biuret” protein complexes ORGANOMETALL IC "B IURET" PROTEIN COMPLEXES K. T. Porosh in Work on organometal l ic "biruet" protein complexes is (/documents/organometallic-biuret-proteinacquiring ever increasing signif icance… complexes.html)
Argricultural Products Analysis, Colorimetric Determination of Biuret drnong phosphorus content of the grain and the various quality measures was toward improved malting quality. All correlations of phosphorus (/documents/argricultural-products-analysis-colorimetriccontent of the grain with the… determination-of-biuret.html)
Metallo-organic “biuret” complexes of protein breakdown products L |ETALLD-ORGANIC '~8 IURET" COMPLEXFSOF PROT£1bt OREAKDOWN PRODUCTS K. T . Pototh in The lp~dy of ~he (/documents/metallo-organic-biuret-complexes-of-proteinprc~ce ,~e~ and lwoduct, of b~eaFdown of pr~teim L~ attracting… breakdown-products.html)
Protein Determination_ Lowry Method 02/10/13 Protein Determination: Lowry Method www.bio.davidson.edu/people/jowilliamson/techniques/protocolweek5.html 1/2 BIOLOGY 371 INDEPENDENT RESEARCH WEEK 5 Protein (/documents/protein-determination-lowry-method.html) Determination…
Penentuan Kadar Protein Secara Biuret
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I.Nomor Percobaan: VI II.Nama Percobaan: Penentuan Kadar Protein Secara Biuret III.Tujuan Percobaan: Untuk mengetahui kadar (/documents/penentuan-kadar-protein-secaraprotein dalamsuatu sampel berdasarkan serapan…
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Biokimia(Revisi)-Penentuan Konsentrasi Protein Cara Biuret penentuan konsentrasi protein cara biuret (/documents/biokimiarevisi-penentuankonsentrasi-protein-cara-biuret.html)
Analytical application of the urease method for determination of urea in the presence of biuret Short communications 1477 8. W. A. Landmann, J. Assoc. Ojic. Anal. Chemists, 1966,49,875. 9. E. Szekely, Talanta, 1968,15,795. 10. B. F. Rider et M. G. Mellon, Ind. Eng.… (/documents/analytical-application-of-the-urease-method-fordetermination-of-urea-in-the.html)
Drawbacks in the use of the Biuret method for determination of the same protein in differently treated fish samples Technical Note Drawbacks in the use of the Biuret Method for Determination of the Same Protein in Differently Treated Fish Samples (/documents/drawbacks-in-the-use-of-the-biuret-method-forThere was a considerable difference in… determination-of-the-same-protein.html)
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