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A Neutrophil Multitarget Functional Bioassay to Detect Anti-inflammatory Natural Products Senia Johansson,Ulf Göransson,Teus Luijendijk,Anders Backlund,Per Claeson, andLars Bohlin* Division of Pharmacognosy, Department of Medicinal Chemistry, Biomedical Centre, Uppsala University, PO Box 574, S751 23 Uppsala, Sweden
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J. Nat. Prod., 2002, 65 (1), pp 32–41 DOI: 10.1021/np010323o Publication Date (Web): December 29, 2001 Copyright © 2002 American Chemical Society and American Society of Pharmacognosy
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A multitarget functional bioassay was optimized as a method for detecting substances
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interacting with the inflammatory process of activated neutrophil granulocytes, mainly to release
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elastase detected by p-nitroanilide (pNA) formation. Using this bioassay, 100 fractionated
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extracts of 96 plants were screened, with results presented in a manner that links recorded
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biological activity to phylogenetic information. The plants were selected to represent a major part
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of the angiosperms, with emphasis on medicinal plants, Swedish anti-inflammatory plants, and plants known to contain peptides. Of the tested extracts, 41% inhibited pNA formation more than
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60%, and 3% stimulated formation. The extract of Digitalis purpurea enhanced pNA formation, and digitoxin, the active compound, was isolated and identified. Plant extracts that exhibited
potent nonselective inhibition (>80% inhibition) were evaluated further for direct inhibition of
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isolated elastase and trypsin enzyme. The inhibitory effect of most tested extracts on the isolated enzyme elastase was similar to that of PAF- and fMLP-induced pNA formation. Retrieve Detailed Record of this Article
Compared to trypsin, inhibition of elastase by extracts of Rubus idaeus and Tabernaemontana dichotoma was significantly higher (80% and 99%, respectively). Inhibition of trypsin by the
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extract of Reseda luteola was high (97%). Orders such as Lamiales and Brassicales were shown
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to include a comparably high proportion of plants with inhibitory extracts.
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Applying label-free dynamic mass redistribution assay for studying endogenous FPR1 receptor signalling in human neutrophils Hanna B. Christensen, David E. Gloriam, Daniel Sejer Pedersen, Jack B. Cowland, Niels Borregaard, Hans BräunerOsborne Journal of Pharmacological and Toxicological Methods 2017 88, 72-78
Natural Products as Source of Anti-Inflammatory Drugs Shivaprasad H. Venkatesha, Bodhraj Acharya, Kamal D. Moudgil 2017,1661-1690
Article Views: 611 Times Received 29 June 2001 Published online 29 December 2001 Published in print 1 January 2002 Learn more about these metrics
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Gastroprotective and antielastase effects of protein inhibitors from Erythrina velutina seeds in an experimental ulcer model
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