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Toggle navigation Topics by WorldWideScience.org Home About News Advanced Search Contact Us Site Map Help Sample records for mel treatment significantly « 1 2 3 4 5 » 1. Microgravity Emissions Laboratory (MEL) Data.gov (United States) Federal Laboratory Consortium — The Microgravity Emissions Laboratory (MEL) utilizes a low-frequency acceleration measurement system for the characterization of rigid body inertial forces generated... 2. Heat Sensitivity of wMel Wolbachia during Aedes aegypti Development. Directory of Open Access Journals (Sweden) Jill N Ulrich 2016-07-01 Full Text Available The wMel strain of Wolbachia bacteria is known to prevent dengue and Zika virus transmission in the mosquito vector Aedes aegypti. Accordingly, the release of wMel-infected A. aegypti in endemic regions has been recommended by the World Health Organization as a potential strategy for controlling dengue and Zika outbreaks. However, the utility of this approach could be limited if high temperatures in the aquatic habitats where A. aegypti develop are detrimental to Wolbachia. We exposed wMel-infected A. aegypti eggs and larvae to fluctuating daily temperatures of 30-40°C for three, five, or seven days during their development. We found that Wolbachia levels in females emerging from heat treatments were significantly lower than in the controls that had developed at 20-30°C. Notably, seven days of high temperatures starting at the egg stage reduced Wolbachia levels in emerging females to less than 0.1% of the wMel control levels. However, after adult females returned to 20-30°C for 4-7 days, they experienced differing degrees of Wolbachia recovery. Our findings suggest that the spread of Wolbachia in wild A. aegypti populations and any consequent protection from dengue and Zika viruses might be limited in ecosystems that experience periods of extreme heat, but Wolbachia levels recover partially after temperatures return to normal. 3. Heat Sensitivity of wMel Wolbachia during Aedes aegypti Development. Science.gov (United States) Ulrich, Jill N; Beier, John C; Devine, Gregor J; Hugo, Leon E 2016-07-01 The wMel strain of Wolbachia bacteria is known to prevent dengue and Zika virus transmission in the mosquito vector Aedes aegypti. Accordingly, the release of wMel-infected A. aegypti in endemic regions has been recommended by the World Health Organization as a potential strategy for controlling dengue and Zika outbreaks. However, the utility of this approach could be limited if high temperatures in the aquatic habitats where A. aegypti develop are detrimental to Wolbachia. We exposed wMel-infected A. aegypti eggs and larvae to fluctuating daily temperatures of 30-40°C for three, five, or seven days during their development. We found that Wolbachia levels in females emerging from heat treatments were significantly lower than in the controls that had developed at 20-30°C. Notably, seven days of high temperatures starting at the egg stage reduced Wolbachia levels in emerging females to less than 0.1% of the wMel control levels. However, after adult females returned to 20-30°C for 4-7 days, they experienced differing degrees of Wolbachia recovery. Our findings suggest that the spread of Wolbachia in wild A. aegypti populations and any consequent protection from dengue and Zika viruses might be limited in ecosystems that experience periods of extreme heat, but Wolbachia levels recover partially after temperatures return to normal. 4. Mel'nikov method revisited OpenAIRE Cicogna, G.; Santoprete, M. 2002-01-01 We illustrate a completely analytic approach to Mel'nikov theory, which is based on a suitable extension of a classical method, and which is parallel and -- at least in part -complementary to the standard procedure. This approach can be also applied to some ``degenerate'' situations, as to the case of nonhyperbolic unstable points, or of critical points located at the infinity (thus giving rise to unbounded orbits, e.g. the Keplerian parabolic orbits), and it is naturally ``compatible'' wit... 5. Validating tyrosinase homologue melA as a photoacoustic reporter gene for imaging Escherichia coli Science.gov (United States) Paproski, Robert J.; Li, Yan; Barber, Quinn; Lewis, John D.; Campbell, Robert E.; Zemp, Roger 2015-10-01 To understand the pathogenic processes for infectious bacteria, appropriate research tools are required for replicating and characterizing infections. Fluorescence and bioluminescence imaging have primarily been used to image infections in animal models, but optical scattering in tissue significantly limits imaging depth and resolution. Photoacoustic imaging, which has improved depth-to-resolution ratio compared to conventional optical imaging, could be useful for visualizing melA-expressing bacteria since melA is a bacterial tyrosinase homologue which produces melanin. Escherichia coli-expressing melA was visibly dark in liquid culture. When melA-expressing bacteria in tubes were imaged with a VisualSonics Vevo LAZR system, the signal-to-noise ratio of a 9× dilution sample was 55, suggesting that Ëœ20 bacteria cells could be detected with our system. Multispectral (680, 700, 750, 800, 850, and 900 nm) analysis of the photoacoustic signal allowed unmixing of melA-expressing bacteria from blood. To compare photoacoustic reporter gene melA (using Vevo system) with luminescent and fluorescent reporter gene Nano-lantern (using Bruker Xtreme In-Vivo system), tubes of bacteria expressing melA or Nano-lantern were submerged 10 mm in 1% Intralipid, spaced between bioluminescence and fluorescence imaging could not resolve the two tubes of Nano-lantern-expressing bacteria even when the tubes were spaced 10 mm from each other. After injecting 100-μL of melA-expressing bacteria in the back flank of a chicken embryo, photoacoustic imaging allowed visualization of melAexpressing bacteria up to 10-mm deep into the embryo. Photoacoustic signal from melA could also be separated from deoxy- and oxy-hemoglobin signal observed within the embryo and chorioallantoic membrane. Our results suggest that melA is a useful photoacoustic reporter gene for visualizing bacteria, and further work incorporating photoacoustic reporters into infectious bacterial strains is warranted. 6. Application of wMelPop Wolbachia Strain to Crash Local Populations of Aedes aegypti. Directory of Open Access Journals (Sweden) Scott A Ritchie Full Text Available The endosymbiotic bacteria Wolbachia pipientis (wMel strain has been successfully established in several populations of Aedes aegypti, the primary dengue vector. The virulent Wolbachia strain wMelPop is known to cause several pathological impacts (increased egg mortality, life shortening, etc. reducing overall fitness in the mosquito Ae. aegypti. Increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populations. We tested this application under semi-field cage conditions. First, we determined that wMelPop infection significantly reduced the survival of desiccation-resistant eggs of the dengue vector Ae. aegypti, with shade and temperature having a significant impact; nearly all wMelPop-infected eggs failed to hatch after 6 and 10 weeks in summer and winter conditions, respectively. In laboratory selection experiments we found that egg desiccation resistance can be increased by selection, and that this effect of wMelPop infection is due to the nuclear background of the host rather than Wolbachia. We then conducted an invasion of wMelPop within a semi-field cage using sustained weekly releases of wMelPop infected mosquitoes, with fixation achieved after 9 weeks. The egg populations wMelPop infected and an uninfected control were then subjected to a simulated prolonged monsoonal dry season (2.5 months before flooding to induce hatching. The wMelPop infected eggs suffered significantly greater mortality than the controls, with only 0.67% and 4.35% of respective infected and uninfected eggs held in 99% shade hatching after 80 days. These studies suggest that wMelPop could be used to locally eliminate populations of Ae. aegypti that are exposed to prolonged dry conditions, particularly if combined with vector control. 7. Application of wMelPop Wolbachia Strain to Crash Local Populations of Aedes aegypti. Directory of Open Access Journals (Sweden) Scott A Ritchie Full Text Available The endosymbiotic bacteria Wolbachia pipientis (wMel strain has been successfully established in several populations of Aedes aegypti, the primary dengue vector. The virulent Wolbachia strain wMelPop is known to cause several pathological impacts (increased egg mortality, life shortening, etc. reducing overall fitness in the mosquito Ae. aegypti. Increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populations. We tested this application under semi-field cage conditions. First, we determined that wMelPop infection significantly reduced the survival of desiccation-resistant eggs of the dengue vector Ae. aegypti, with shade and temperature having a significant impact; nearly all wMelPop-infected eggs failed to hatch after 6 and 10 weeks in summer and winter conditions, respectively. In laboratory selection experiments we found that egg desiccation resistance can be increased by selection, and that this effect of wMelPop infection is due to the nuclear background of the host rather than Wolbachia. We then conducted an invasion of wMelPop within a semi-field cage using sustained weekly releases of wMelPop infected mosquitoes, with fixation achieved after 9 weeks. The egg populations wMelPop infected and an uninfected control were then subjected to a simulated prolonged monsoonal dry season (2.5 months before flooding to induce hatching. The wMelPop infected eggs suffered significantly greater mortality than the controls, with only 0.67% and 4.35% of respective infected and uninfected eggs held in 99% shade hatching after 80 days. These studies suggest that wMelPop could be used to locally eliminate populations of Ae. aegypti that are exposed to prolonged dry conditions, particularly if combined with vector control. 8. Validating tyrosinase homologue melA as a photoacoustic reporter gene for imaging Escherichia coli. Science.gov (United States) Paproski, Robert J; Li, Yan; Barber, Quinn; Lewis, John D; Campbell, Robert E; Zemp, Roger 2015-10-01 To understand the pathogenic processes for infectious bacteria, appropriate research tools are required for replicating and characterizing infections. Fluorescence and bioluminescence imaging have primarily been used to image infections in animal models, but optical scattering in tissue significantly limits imaging depth and resolution. Photoacoustic imaging, which has improved depth-to-resolution ratio compared to conventional optical imaging, could be useful for visualizing melA-expressing bacteria since melA is a bacterial tyrosinase homologue which produces melanin. Escherichia coli-expressing melA was visibly dark in liquid culture. When melA-expressing bacteria in tubes were imaged with a VisualSonics Vevo LAZR system, the signal-to-noise ratio of a 9×dilution sample was 55, suggesting that ∼20 bacteria cells could be detected with our system. Multispectral (680, 700, 750, 800, 850, and 900 nm) analysis of the photoacoustic signal allowed unmixing of melA-expressing bacteria from blood. To compare photoacoustic reporter gene melA (using Vevo system) with luminescent and fluorescent reporter gene Nano-lantern (using Bruker Xtreme In-Vivo system), tubes of bacteria expressing melA or Nano-lantern were submerged 10 mm in 1% Intralipid, spaced between Photoacoustic imaging could resolve the two tubes of melA-expressing bacteria even when the tubes were less than 1 mm from each other, while bioluminescence and fluorescence imaging could not resolve the two tubes of Nano-lantern-expressing bacteria even when the tubes were spaced 10 mm from each other. After injecting 100-μL of melA-expressing bacteria in the back flank of a chicken embryo, photoacoustic imaging allowed visualization of melA-expressing bacteria up to 10-mm deep into the embryo. Photoacoustic signal from melA could also be separated from deoxy- and oxy-hemoglobin signal observed within the embryo and chorioallantoic membrane. Our results suggest that melA is a useful photoacoustic reporter gene 9. Establishment of the cytoplasmic incompatibility-inducing Wolbachia strain wMel in an important agricultural pest insect Science.gov (United States) Zhou, Xiao-Fei; Li, Zheng-Xi 2016-01-01 The wMel Wolbachia strain was known for cytoplasmic incompatibility (CI)-induction and blocking the transmission of dengue. However, it is unknown whether it can establish and induce CI in a non-dipteran host insect. Here we artificially transferred wMel from Drosophila melanogaster into the whitefly Bemisia tabaci. Fluorescence in situ hybridisation demonstrated that wMel had successfully transfected the new host. Reciprocal crossing was conducted with wMel-transfected and wild-type isofemale lines, indicating that wMel could induce a strong CI without imposing significant cost on host fecundity. We then determined the maternal transmission efficiency of wMel in the offspring generations, showing a fluctuating trend over a period of 12 generations. We thus detected the titre of wMel during different developmental stages and in different generations by using real-time quantitative PCR, revealing a similar fluctuating mode, but it was not significantly correlated with the dynamics of transmission efficiency. These results suggest that wMel can be established in B.tabaci, a distantly related pest insect of agricultural importance; moreover, it can induce a strong CI phenotype in the recipient host insect, suggesting a potential for its use in biological control of B. tabaci. PMID:27982076 10. Substâncias voláteis em mel floral e mel de melato Directory of Open Access Journals (Sweden) CAMPOS Gisélia 2000-01-01 Full Text Available Embora pareça existir um "flavor" caracterÃstico de mel, a grande variedade de flores disponÃveis para a abelha, possibilita uma grande diversidade de flavor e aroma, indicando a presença de vários componentes voláteis. Alguns destes dependem da fisiologia da abelha, dos procedimentos após a colheita e no mel de melato há também a interferência de insetos sugadores e das formigas. Várias substâncias voláteis já foram identificadas, sendo algumas caracterÃsticas de determinados méis uniflorais. Com o objetivo de encontrar uma substância volátil caracterÃstica do mel de melato, seis amostras deste tipo de mel e seis amostras de mel floral foram analisadas usando extração por arraste de gás hidrogênio e cromatografia a gás acoplada a espectrometria de massas. Ãcido acético foi encontrado em quatro amostras de mel de melato e em uma amostra de mel floral porém, com menor abundância. 11. Mel-18 gene and neoplasms%Mel-18åŸºå› ä¸Žè‚¿ç˜¤ Institute of Scientific and Technical Information of China (English) 许å¦å®—; 曹京燕; äºŽé› 2013-01-01 é»‘è‰²ç´ ç˜¤æ ¸è›‹ç™½-18(Mel-18)åŸºå› æ˜¯å¤šæ¢³åŸºå› (PcG)家æ—æ ¸å¿ƒæˆå‘˜ä¹‹ä¸€,在胚胎形æˆã€ç»†èƒžç”Ÿé•¿å’Œå¢žæ®–ã€å¹²ç»†èƒžè‡ªæˆ‘更新的调节ä¸å‘挥é‡è¦ä½œç”¨.Mel-18åŸºå› è¡¨è¾¾å¼‚å¸¸ä¸Žäººç±»è‚¿ç˜¤çš„å‘生ã€å‘展过程有关.ä½œä¸ºè‚¿ç˜¤æŠ‘åˆ¶åŸºå› ,Mel-18åŸºå› é€šè¿‡å¯¹B淋巴瘤Mo-MLVæ’å… ¥åŒºåŸŸ1(Bmi-1)å’Œé«“ç»†èƒžç™ŒåŸºå› (c-myc)的转录抑制而抑制肿瘤增长.Mel-18表达在许多肿瘤细胞的转录水平和翻译水平å‡å°‘,å… ¶ä¸åŒ…括乳腺癌ã€å‰åˆ—腺癌和胃癌ç‰è‚¿ç˜¤,有望æˆä¸ºä¸€ç§æ–°çš„肿瘤预åŽæ ‡å¿—物.%Mel-18 gene is one of the core members of the PcG (polycomb group) family,which plays an important role in embryogenesis,cell growth and proliferation and self-renewal of stem cells.Mel-18 gene expressing abnormally has been related to human tumorigenesis,development process.Mel-18 serves as a tumor suppressor gene and inhibits tumor growth through transcriptional repression of Bmi-1 and cmyc.Mel-18 expression is decreased at transcriptional and translational levels in most human cancers including breast cancer,prostate cancer,and gastric cancer and other tumors.Mel18 is expected to become a prognostic marker for human cancers. 12. Assessing and Reducing Miscellaneous Electric Loads (MELs) in Lodging Energy Technology Data Exchange (ETDEWEB) Rauch, Emily M. 2011-09-01 Miscellaneous electric loads (MELs) are the loads outside of a building's core functions of heating, ventilating, air conditioning, lighting, and water heating. This report reviews methods to reduce MELs in lodging. 13. Adjuvant therapy with high dose vitamin D following primary treatment of melanoma at high risk of recurrence: a placebo controlled randomised phase II trial (ANZMTG 02.09 Mel-D). Science.gov (United States) Saw, Robyn P M; Armstrong, Bruce K; Mason, Rebecca S; Morton, Rachael L; Shannon, Kerwin F; Spillane, Andrew J; Stretch, Jonathan R; Thompson, John F 2014-10-24 Patients with primary cutaneous melanomas that are ulcerated and >2 mm in thickness, >4 mm in thickness and those with nodal micrometastases at diagnosis, have few options for adjuvant treatment. Recent studies have suggested a role for vitamin D to delay melanoma recurrence and improve overall prognosis. This is a pilot placebo-controlled randomised phase II trial to assess the feasibility, safety and toxicity of an oral loading dose of Vitamin D (500,000 IU) followed by an oral dose of 50,000 IU of Vitamin D monthly for 2 years in patients who have been treated for cutaneous melanoma by wide excision of the primary. Patients aged 18-79 years who have completed primary surgical treatment and have Stage IIb, IIc, IIIa (N1a, N2a) or IIIb (N1a, N2a) disease are eligible for randomisation 2:1 to active treatment or placebo. The primary endpoints are sufficiency of dose, adherence to study medication and safety of the drug. The secondary endpoints are participation and progression free survival. The study has been approved by the Ethics Review Committee (RPAH Zone) of the Sydney Local Health District, protocol number X09-0138. Effective, non-toxic adjuvant therapy for high risk primary melanoma is not currently available. Favorable outcomes of this phase II study will form the basis for a multi-centre phase III study to assess whether the addition of oral high-dose vitamin D therapy in patients who have completed primary treatment for melanoma and are at high risk of recurrence will: 1. prolong time to recurrence within 5 years 2. improve overall survival at 5 years and 3. be both safe and tolerable. 62 patients have been randomised since the study commenced in December 2010. Target accrual for the study has been met with 75 patients randomised between December 2010 and August 2014.The Mel-D trial is conducted by the Australia and New Zealand Melanoma Trials Group (ANZMTG 02.09) TRIAL REGISTRATION: Australia and New Zealand Clinical Trials Registry (ANZCTR) ACTRN 14. Control of enzymatic degradation of biodegradable polymers by treatment with biosurfactants, mannosylerythritol lipids, derived from Pseudozyma spp. yeast strains. Science.gov (United States) Fukuoka, Tokuma; Shinozaki, Yukiko; Tsuchiya, Wataru; Suzuki, Ken; Watanabe, Takashi; Yamazaki, Toshimasa; Kitamoto, Dai; Kitamoto, Hiroko 2016-02-01 Cutinase-like esterase from the yeasts Pseudozyma antarctica (PaE) shows strong degradation activity in an agricultural biodegradable plastic (BP) model of mulch films composed of poly(butylene succinate-co-adipate) (PBSA). P. antarctica is known to abundantly produce a glycolipid biosurfactant, mannosylerythritol lipid (MEL). Here, the effects of MEL on PaE-catalyzed degradation of BPs were investigated. Based on PBSA dispersion solution, the degradation of PBSA particles by PaE was inhibited in the presence of MEL. MEL behavior on BP substrates was monitored by surface plasmon resonance (SPR) using a sensor chip coated with polymer films. The positive SPR signal shift indicated that MEL readily adsorbed and spread onto the surface of a BP film. The amount of BP degradation by PaE was monitored based on the negative SPR signal shift and was decreased 1.7-fold by MEL pretreatment. Furthermore, the shape of PBSA mulch films in PaE-containing solution was maintained with MEL pretreatment, whereas untreated films were almost completely degraded and dissolved. These results suggest that MEL covering the surface of BP film inhibits adsorption of PaE and PaE-catalyzed degradation of BPs. We applied the above results to control the microbial degradation of BP mulch films. MEL pretreatment significantly inhibited BP mulch film degradation by both PaE solution and BP-degradable microorganism. Moreover, the degradation of these films was recovered after removal of the coated MEL by ethanol treatment. These results demonstrate that the biodegradation of BP films can be readily and reversibly controlled by a physical approach using MEL. 15. 艾ç¸å¯¹å®žéªŒæ€§ç±»é£Žæ¹¿æ€§å…³èŠ‚ç‚Žå¤§é¼ æµ·é©¬GRã€MEL1B表达影å“çš„ç ”ç©¶%Research of Moxibustion Influence on GR and MEL1B Expressions in Hippocampus of Experimental RA Rats Institute of Scientific and Technical Information of China (English) 周海燕; 刘æ—å…‰; é«˜éª 2012-01-01 Objective: To explore the mechanism of moxibustion treating hippocampal function of experimental rheumatoid arthritis (RA)rats. Methods:Sprague-Dawley(SD)rats were randomly divided into normal control group,model group and moxibustion group. RA animal models were made by Freunds Complete Adjuvant (FCA) ,Shenshu(BL23)and Zusanli(ST36) acupoints were selected to make moxibustion. The hippocampal glucocorticoid receptor(GR) expression was detected by immunohistochemi-cal method,and the hippocampal melatonin receptor 1B( MEL1B) expression by in situ hybridization method. Results: Compared with normal group,the GR expression of model group had an increasing trend(P >0. 05) ,that of moxibustion group was significantly lower than that of model group(P 5 at admission, 25.6 % of them had INR > 10. The mortality of patients using oral anticoagulants and INR > 5 was 17.1 %.Conclusions: Upper gastrointestinal hemorrhage is a serious complication of different medications, particularly in elderly patients. Safe use of anticoagulant therapy is based on careful selection of patients and correct intake of the prescribed drugs. 30. TINEA CAPITIS– SIGNIFICANCE OF TIMELY TREATMENT TO PREVENT SCARRING ALOPECIA Directory of Open Access Journals (Sweden) Sandrina Carvalho 2016-09-01 Conclusion: The treatment of tinea capitis is simple and effective. Timely identification and treatment are essential to prevent the formation of a cicatricial alopecia, often disturbing for the patient. 31. DETERMINAÇÃO DA QUALIDADE DO MEL Directory of Open Access Journals (Sweden) Crispin Humberto GARCIA-CRUZ 2009-07-01 Full Text Available RESUMO: Foi determinada a qualidade do mel por meio da realização de análises microbiológicas e quÃmicas de vinte amostras de mel (100% de consumo imediato comercializadas no varejo por pequenos apicultores e feirantes da região de São José do Rio Preto, Estado de São Paulo. Foi realizada a contagem de bactérias aeróbias termófilas; contagem de bolores e leveduras e a pesquisa de Salmonella sp. Os resultados das análises microbiológicas das amostras de mel mostraram que as contagens de bactérias aeróbias mesófilas variaram de 0,5 x 101 a 2,2 x 102 UFC/g; já as contagens de bactérias aeróbias termófilas variaram de 0,5 x 101 a 1,4 x 102 UFC/g. Foi detectada a presença de salmonella sp, em apenas uma das amostras analisadas (5%, não atendendo, portanto, ao padrão de ausência em 25g de amostra. Com relação à s análises quÃmicas verificou-se que a média da acidez titulável foi de 2,28% (v/p com variação de 1,19% a 3,33% (v/p. De acordo com os resultados obtidos 40% das amostras foram classificadas como mel de mesa e 60% como mel industrial. A análise global dos resultados revelou a adição de glicose comercial em algumas amostras analisadas. PALAVRAS-CHAVE: Mel; qualidade; microbiologia; análise. 32. Musical Instrument Identification using Multiscale Mel-frequency Cepstral Coefficients DEFF Research Database (Denmark) Sturm, Bob L.; Morvidone, Marcela; Daudet, Laurent 2010-01-01 We investigate the benefits of evaluating Mel-frequency cepstral coefficients (MFCCs) over several time scales in the context of automatic musical instrument identification for signals that are monophonic but derived from real musical settings. We define several sets of features derived from MFCCs... 33. Successful treatment with 308-nm monochromatic excimer light and subsequent tacrolimus 0.03% ointment in refractory plasma cell cheilitis. Science.gov (United States) Yoshimura, Kazuhiro; Nakano, Shunji; Tsuruta, Daisuke; Ohata, Chika; Hashimoto, Takashi 2013-06-01 Plasma cell cheilitis is a chronic inflammatory disease that presents with erythema, erosions, ulcers and occasional nodules within the mucosa, including the lips. It is histopathologically characterized by dense plasma cell infiltration in the lamina propria of the mucous membranes. Several treatments for plasma cell cheilitis have been reported, including topical steroids, topical antibiotics or topical tacrolimus. However, 308-nm monochromatic excimer light (MEL) has never been reported as a treatment option, while it was reported to be very effective in treating erosive oral lichen planus. We report a 62-year-old man who had chronic plasma cell cheilitis on the lower lip, which was refractory to topical and systemic corticosteroid. The lesion and severe pain were significantly improved by the treatment with nine sessions of 308-nm MEL twice per week with a total dose of 1120 mJ/cm(2). However, the lesion gradually worsened after treatment frequency was reduced to once per month. Subsequent tacrolimus 0.03% ointment cleared the lesion completely in a month and no recurrence was observed a year later. Refractory plasma cell cheilitis and concomitant severe pain quickly responded to 308-nm MEL when administrated twice per week. Because the long interval between each MEL treatment seemed ineffective to improve the lesion, appropriate frequency and adequate total dose of MEL treatment may be necessary for a successful treatment. 34. Cough Recognition Based on Mel Frequency Cepstral Coefficients and Dynamic Time Warping Science.gov (United States) Zhu, Chunmei; Liu, Baojun; Li, Ping Cough recognition provides important clinical information for the treatment of many respiratory diseases, but the assessment of cough frequency over a long period of time remains unsatisfied for either clinical or research purpose. In this paper, according to the advantage of dynamic time warping (DTW) and the characteristic of cough recognition, an attempt is made to adapt DTW as the recognition algorithm for cough recognition. The process of cough recognition based on mel frequency cepstral coefficients (MFCC) and DTW is introduced. Experiment results of testing samples from 3 subjects show that acceptable performances of cough recognition are obtained by DTW with a small training set. 35. Evidence-based treatment of stuttering: II. Clinical significance of behavioral stuttering treatments. Science.gov (United States) Finn, Patrick 2003-01-01 An evidence-based framework can be described as an empirically-driven, measurement-based, client-sensitive approach for selecting treatments. It is believed that using such a framework is more likely to result in a clinically significant outcome. For this paper, a clinically significant outcome was defined as a meaningful treatment change. It was suggested that there are at least three groups for whom a treatment's outcome is meaningful. These groups include clinicians/clinical researchers, the clients, and relevant others who have some interest in the outcome (e.g., parents of a child who stutters). The meaning and measurement of clinical significance was discussed for each of these three groups, based on research from the behavioral stuttering treatment literature. The reader will learn about and be able to (1) broadly define a clinically significant outcome and identify some of the groups who are interested in such an outcome and (2) describe how clinical significance has been evaluated in stuttering treatment within an evidence-based framework. 36. Significance of "Deqi" response in acupuncture treatment: myth or reality. Science.gov (United States) Zhou, Wei; Benharash, Peyman 2014-08-01 Acupuncture has been practiced in China for over 2000 years to treat a variety of diseases based on the "meridian theory," as described in the "Yellow Emperor's Classics of Internal Medicine." Deqi refers to the excitation of qi or vital energy inside meridians by acupuncture needle stimulation. Patients often experience multidimensional and intense needling sensations such as numbness, soreness, distention, heaviness, dull pain, and sharp pain during acupuncture stimulation. Deqi is considered as an important parameter in the process of achieving therapeutic effectiveness in acupuncture treatment. Understanding this phenomenon from neurophysiological aspects is important for clinical practice and enables practitioners to perform quantitative acupuncture evaluation to obtain a reliable prognosis of acupuncture treatment. This review paper describes our current knowledge and understanding of Deqi from a physiological aspect. Copyright © 2014. Published by Elsevier B.V. 37. The Environmental Significance of Bioindicators in Sewage Treatment Directory of Open Access Journals (Sweden) Judit Németh-Katona 2008-06-01 Full Text Available The presentation is about the significance of the bioindicators concerningenvironmental protection within the process of cleaning sewage. The existence of one ormulti-celled organisms indicates the presence, condition or absence of certain parts of thewater cleaning process. This way the optimal operation of the purifying appliances can bechecked continuously and controlled in an environment friendly way. 38. Inpatient Treatment for Adolescents with Anorexia Nervosa: Clinical Significance and Predictors of Treatment Outcome. Science.gov (United States) Schlegl, Sandra; Diedrich, Alice; Neumayr, Christina; Fumi, Markus; Naab, Silke; Voderholzer, Ulrich 2016-05-01 This study evaluated the clinical significance as well as predictors of outcome for adolescents with severe anorexia nervosa (AN) treated in an inpatient setting. Body mass index (BMI), eating disorder (ED) symptoms [Eating Disorder Inventory-2 (EDI-2)], general psychopathology and depression were assessed in 238 patients at admission and discharge. BMI increased from 14.8 + 1.2 to 17.3 + 1.4 kg/m(2). Almost a fourth (23.6%) of the patients showed reliable changes, and 44.7% showed clinically significant changes (EDI-2). BMI change did not significantly differ between those with reliable or clinically significant change or no reliable change in EDI-2. Length of stay, depression and body dissatisfaction were negative predictors of a clinically significant change. Inpatient treatment is effective in about two thirds of adolescents with AN and should be considered when outpatient treatment fails. About one third of patients showed significant weight gain, but did not improve regarding overall ED symptomatology. Future studies should focus on treatment strategies for non-responders. 39. Exosomes and Their Significance in Diagnosis and Treatment of Tumors Directory of Open Access Journals (Sweden) Jian WANG 2015-12-01 Full Text Available Abstract In the research field of biological markers for tumor diagnosis, the appearance of exosomes has resolved the problem that RNA molecules can be easily degraded. Exosomes carry various RNAs and can protect them from being degraded. They are defined as polymorphism vesicle-like corpuscles (diameter: 30-100 nm derived from late endosome or multi-vesicular endosomes in cellular endocytosis system, which contain abundant biological information, including multiple lipids, proteins and nucleic acids, etc. Exosomes are extracellular nanoscale vesicae formed in a series of regulating process of cellular “endocytosis-fusion-excretionâ€, and they carry proteins and transport RNAs, thus playing an important role in the intercellular material and informational transduction. There are still large amount of mRNAs and miRNAs in exosomes. Exosomes can not only protect in-vitro RNA stability, but also transfer RNA to specific target cells as effective carriers so as to play their regulatory function. Exosomes realize their biological information exchanges and transition via endocrine, paracrine and autocrine, and regulate cellular biological activities through direct action on superficial signal molecules or extracellular release and membrane fusion of biological active ingredients. They can directly act on tumors to impact tumor progression, or improve tumor angiogenesis and metastasis by regulating immunological function. Additionally, they can also be used for tumor diagnosis. Therefore, this study mainly summarized the biological characteristics of exosomes and their application in the regulation, diagnosis and treatment of tumors, hoping to provide references for the application of exosomes in tumors. 40. The wMel Strain of Wolbachia Reduces Transmission of Chikungunya Virus in Aedes aegypti Science.gov (United States) Aliota, Matthew T.; Walker, Emma C.; Uribe Yepes, Alexander; Dario Velez, Ivan; Christensen, Bruce M.; Osorio, Jorge E. 2016-01-01

Background New approaches to preventing chikungunya virus (CHIKV) are needed because current methods are limited to controlling mosquito populations, and they have not prevented the invasion of this virus into new locales, nor have they been sufficient to control the virus upon arrival. A promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium Wolbachia into Aedes aegypti mosquitoes. This primarily has been proposed as a tool to control dengue virus (DENV) transmission; however, evidence suggests Wolbachia infections confer protection for Ae. aegypti against CHIKV. Although this approach holds much promise for limiting virus transmission, at present our understanding of the ability of CHIKV to infect, disseminate, and be transmitted by wMel-infected Ae. aegypti currently being used at Wolbachia release sites is limited. Methodology/Principal Findings Using Ae. aegypti infected with the wMel strain of Wolbachia that are being released in Medellin, Colombia, we report that these mosquitoes have reduced vector competence for CHIKV, even with extremely high viral titers in the bloodmeal. In addition, we examined the dynamics of CHIKV infection over the course of four to seven days post feeding. Wolbachia-infected mosquitoes remained non-infective over the duration of seven days, i.e., no infectious virus was detected in the saliva when exposed to bloodmeals of moderate viremia, but CHIKV-exposed, wild type mosquitoes did have viral loads in the saliva consistent with what has been reported elsewhere. Finally, the presence of wMel infection had no impact on the lifespan of mosquitoes as compared to wild type mosquitoes following CHIKV infection. Conclusions/Significance These results could have an impact on vector control strategies in areas where Ae. aegypti are transmitting both DENV and CHIKV; i.e., they argue for further exploration, both in the laboratory and the field, on the feasibility of expanding this « 1 2 3 4 5 » « 1 2 3 4 5 » 41. Monochromatic excimer light versus combination of topical steroid with vitamin D3 analogue in the treatment of nonsegmental vitiligo: a randomized blinded comparative study. Science.gov (United States) Abdel Latif, Azmy Ahmed; Ibrahim, Shady Mahmoud Attia 2015-01-01 Vitiligo is a difficult disease to treat, socially stigmatizing its patients. Monochromatic excimer light (MEL) was developed for use in dermatology and adapted for the treatment of vitiligo. Comparing the efficacy of MEL versus topical combination therapy of vitamin D3 analogue and steroid in the treatment of nonsegmental vitiligo. Forty-four patients with localized and stable nonsegmental vitiligo participated in the present study. In each patient, two lesions were selected and divided randomly into two groups, group A was treated with daily topical combination of calcipotriol and betamethasone and group B was treated with biweekly sessions of MEL for 3 months. Efficacy based on repigmentation percentages were blindly evaluated by two independent physicians and patient's satisfaction. There was significant improvement in both treatment modalities at the end of the study, but without significant differences in both groups. There was a significant difference between both groups regarding the onset of repigmentation (p-value vitiligo. 42. Usability Evaluation of Multimedia Courseware (MEL-SindD) Science.gov (United States) Yussof, Rahmah Lob; Badioze Zaman, Halimah Constructive evaluations on any software are needed to ensure the effectiveness and usability of the software. This assesment on the multimedia courseware is part of the researcher's study towards the development and usability of the early reading software for students with Down Syndrome (MEL-SindD). This paper will discuss the usability assesment of this courseware, the methods used for the evaluation as well as suitable approaches that can be deployed to evaluate the courseware effectiveness to disabled children. 43. Using Public Participation to Improve MELs Energy Data Collection Energy Technology Data Exchange (ETDEWEB) Cheung, Iris (Hoi Ying); Kloss, Margarita; Brown, Rich; Meier, Alan 2014-03-11 Miscellaneous Electric Loads (MELs) have proliferated in the last decade, and comprise an increasing share of building energy consumption. Because of the diversity of MELs and our lack of understanding about how people use them, large-scale data collection is needed to inform meaningful energy reduction strategies. Traditional methods of data collection, however, usually incur high labor and metering equipment expenses. As an alternative, this paper investigates the feasibility of crowdsourcing data collection to satisfy at least part of the data collection needs with acceptable accuracy. This study assessed the reliability and accuracy of crowdsourced data, by recruiting over 20 volunteers (from the 2012 Lawrence Berkeley Lab, Open House event) to test our crowdsourcing protocol. The protocol asked volunteers to perform the following tasks for three test products with increasing complexity - record power meter and product characteristics, identify all power settings available, and report the measured power. Based on our collected data and analysis, we concluded that volunteers performed reasonably well for devices with functionalities with which they are familiar, and might not produce highly accurate field measurements for complex devices. Accuracy will likely improve when participants are measuring the power used by devices in their home which they know how to operate, by providing more specific instructions including instructional videos. When integrated with existing programs such as the Home Energy Saver tool, crowdsourcing data collection from individual homeowners has the potential to generate a substantial amount of information about MELs energy use in homes. 44. Endoleakage after endovascular treatment of abdominal aortic aneurysms: diagnosis, significance and treatment Energy Technology Data Exchange (ETDEWEB) Golzarian, Jafar [University of Iowa, University of Iowa Heath Care, IA (United States); Valenti, David [McGill University, Royal Victoria Hospital, McGill University Health Centre, Montreal (Canada) 2006-12-15 Endoleak, also called leakage, leak and Perigraft leak, is a major complication and its persistence represents a failure of endovascular aortic aneurysm repair. Its detection and treatment is therefore of primary importance, since endoleak can be associated with pressurization (increase in pressure) of the sac, resulting in expansion and rupture of the aneurysm. The aim of this paper is to discuss the definition, significance, diagnosis and different options to treat endoleak. (orig.) 45. Molecular analysis of the Mycobacterium tuberculosis lux-like mel2 operon. Science.gov (United States) Janagama, Harish K; Tounkang, Sambou; Cirillo, Suat L G; Zinniel, Denise K; Barletta, Raúl G; Cirillo, Jeffrey D 2013-12-01 Using a high throughput genetic strategy, designated Random Inducible Controlled Expression (RICE), we identified the six gene mel2 locus in Mtb and M. marinum. Interestingly, three of the genes present in mel2 have similarities to bioluminescence genes. Similar to other bacterial bioluminescence systems, mel2 facilitates detoxification of reactive oxygen species (ROS). Through the use of thin layer chromatography (TLC) we demonstrate enhanced production of the cell wall virulence lipid, pthiocerol dimycoserosate (PDIM), in a Mtb mel2 mutant relative to the wild type strain in the presence of both H2O2 and diamide oxidative stresses. Furthermore, propionate toxicity assays revealed increased accumulation of triacylglycerol (TAG) in the mel2 mutant relative to wild type. These observations provide the first evidence that mel2 plays a critical role in Mtb lipid biosynthesis. 46. Biocontrole da mancha-aquosa do melão pelo tratamento de sementes com bactérias epifÃticas e endofÃticas Biocontrol of bacterial blotch of melon by seed treatment with epiphytic and endophytic bacteria Directory of Open Access Journals (Sweden) Aldenir de Oliveira 2006-09-01 avenae subsp. citrulli or treating healthy seeds in order to protect the plant challenged later with the pathogen. Melon seeds were microbiolized by immersion in suspensions (A570= 0,7, sown and evaluated for incubation period (PI, incidence (INC, disease severity (SEV and disease severity reduction (RSD. Only microbiolization of artificially infected seed using the endophytic ENM5 (not identified, ENM9 (Bacillus cereus, ENM13 (Bacillus sp., ENM16 (Bacillus cereus, ENM32 (Bacillus subtilis and ENM43 (Bacillus sp., showed potencial for disease control. After in vitro compatibility tests, these strains were reevaluated separately and in pairs, in relation to PI, INC, SEV, RSD, disease index (IDO and area under disease progress curve (AACPD. All treatments differed significantly (P= 0.05 from the control reaching 93.6% of RSD, with emphasis for ENM13 and ENM9 with 7.5 and 7.25 days of PI, 0.22 and 0.22 of SEV, 2.59 and 2.59 of IDO, and 0.22 and 0.39 of AACPD, respectively. In vitro tests to determine the putative mechanisms of action involved in biocontrol were performed. ENM13 and ENM9 solubilized phosphate, ENM5 presented antibiose against A. avenae subsp. citrulli, and ENM43 produced HCN while ENM16 and ENM32 did not show any of the tested mechanisms. 47. Treatment of angular cheilitis. The significance of microbial analysis, antimicrobial treatment, and interfering factors. Science.gov (United States) Ohman, S C; Jontell, M 1988-10-01 This prospective study evaluated the significance of microbial analysis and antimicrobial treatment for the cure of angular cheilitis. Furthermore, various etiologic factors were investigated for their relative effect on the healing process. The study included 1) an open trial with 50 patients infected by Candida albicans and/or Staphylococcus aureus, and 2) an intraindividual comparison of eight patients with bilateral lesions infected by Candida albicans as the only detected pathogen. After a base-line examination the patients received ointments containing nystatin and/or fusidic acid, on the basis of the outcome of an initial microbial analysis. The patients were evaluated clinically, photographed, and examined for microorganisms at different time intervals. Ninety-six per cent of the patients who participated in the open trial had no sign of infection after 42 days of treatment. Lesions in the double-blind study, treated with nystatin, were healed after 28 days, whereas lesions that received placebo persisted throughout the treatment period. Increasing age, dry skin, and extended skinfolds at the corner of the mouth were factors closely related to the length of the healing process. 48. Mel-cepstrum-based steganalysis for VoIP steganography Science.gov (United States) Kraetzer, Christian; Dittmann, Jana 2007-02-01 Steganography and steganalysis in VoIP applications are important research topics as speech data is an appropriate cover to hide messages or comprehensive documents. In our paper we introduce a Mel-cepstrum based analysis known from speaker and speech recognition to perform a detection of embedded hidden messages. In particular we combine known and established audio steganalysis features with the features derived from Melcepstrum based analysis for an investigation on the improvement of the detection performance. Our main focus considers the application environment of VoIP-steganography scenarios. The evaluation of the enhanced feature space is performed for classical steganographic as well as for watermarking algorithms. With this strategy we show how general forensic approaches can detect information hiding techniques in the field of hidden communication as well as for DRM applications. For the later the detection of the presence of a potential watermark in a specific feature space can lead to new attacks or to a better design of the watermarking pattern. Following that the usefulness of Mel-cepstrum domain based features for detection is discussed in detail. 49. Physiological studies in aerobic batch cultivations of Saccharomyces cerevisiae strains harboring the MEL1 gene DEFF Research Database (Denmark) Østergaard, Simon; Roca, Christophe Francois Aime; Ronnow, B. 2000-01-01 Physiological studies of Saccharomyces cerevisiae strains harboring the MEL1 gene were carried out in aerobic batch cultivations on glucose-galactose mixtures and on the disaccharide melibiose, which is hydrolyzed by the enzyme melibiase (Mel1, EC 3.2.1.22) into a glucose and a galactose moiety. ... 50. Eye Blink Imitation Based on Mel Script%基于Mel Script的人眼éšæœºçœ¨åŠ¨è¡Œä¸ºä»¿çœŸç ”究 Institute of Scientific and Technical Information of China (English) æƒ æ™“é’Ÿ 2011-01-01 This paper discusses the method for imitating human eye random blink behavior with Maya Mel Script. The basic rules of human eye blink, eye rigging and wrapping technique in Maya, and the method of imitating eye blink with Mel script without keyframing are analyzed. The advantages and disadvantages of Blendshape and joint rigging in facial animation are discussed. The result shows that the imitation is working, and it saves the animator's time.%为说明如何用Maya Mel Script脚本è¯è¨ €å¯¹äººçœ¼éšæœºçœ¨åŠ¨è¡Œä¸ºè¿›è¡Œä»¿çœŸï¼›æ–‡ç« 分æžäº†äººçœ¼çœ¨åŠ¨çš„行为规律,说明了在Mayaä¸å¯¹çœ¼ç›çš„骨骼绑定和å°è£…æŠ €æœ¯,以åŠåˆ©ç”¨ç‹¬ç«‹äºŽåŠ¨ç”»æ—¶é—´çº¿å’Œå…³é”®å¸§ç¼–辑模å¼çš„Mel Script动画技术模拟人眼的éšæœºçœ¨åŠ¨è¡Œä¸º.并讨论了BlendshapeæŠ €æœ¯å’Œéª¨éª¼ç»‘定结åˆMel Script动画技术在动画效果上的优劣.结果è¯æ˜ŽåŸºäºŽMel Script的人眼éšæœºçœ¨åŠ¨è¡Œä¸ºä»¿çœŸæ˜¯å¯è¡Œçš„,å¯ä»¥èŠ‚çœåŠ¨ç”»åˆ¶ä½œæ—¶é—´. 51. An approach to Mel'nikov theory in celestial mechanics CERN Document Server Cicogna, G 1999-01-01 Using a completely analytic procedure - based on a suitable extension of a classical method - we discuss an approach to the Poincaré-Mel'nikov theory, which can be conveniently applied also to the case of non-hyperbolic critical points, and even if the critical point is located at the infinity. In this paper, we concentrate our attention on the latter case, and precisely on problems described by Kepler-like potentials in one or two degrees of freedom, in the presence of general time-dependent perturbations. We show that the appearance of chaos (possibly including Arnol'd diffusion) can be proved quite easily and in a direct way, without resorting to singular coordinate transformations, such as the McGehee or blowing-up transformations. Natural examples are provided by the classical Gyldén problem, originally proposed in celestial mechanics, but also of interest in different fields, and by the general 3-body problem in classical mechanics. 52. Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation. Directory of Open Access Journals (Sweden) Georgina Gómez-Saldivar 2016-06-01 Full Text Available Nucleoporins are the constituents of nuclear pore complexes (NPCs and are essential regulators of nucleocytoplasmic transport, gene expression and genome stability. The nucleoporin MEL-28/ELYS plays a critical role in post-mitotic NPC reassembly through recruitment of the NUP107-160 subcomplex, and is required for correct segregation of mitotic chromosomes. Here we present a systematic functional and structural analysis of MEL-28 in C. elegans early development and human ELYS in cultured cells. We have identified functional domains responsible for nuclear envelope and kinetochore localization, chromatin binding, mitotic spindle matrix association and chromosome segregation. Surprisingly, we found that perturbations to MEL-28's conserved AT-hook domain do not affect MEL-28 localization although they disrupt MEL-28 function and delay cell cycle progression in a DNA damage checkpoint-dependent manner. Our analyses also uncover a novel meiotic role of MEL-28. Together, these results show that MEL-28 has conserved structural domains that are essential for its fundamental roles in NPC assembly and chromosome segregation. 53. Mel-18 negatively regulates stem cell-like properties through downregulation of miR-21 in gastric cancer. Science.gov (United States) Wang, Xiao-Feng; Zhang, Xiao-Wei; Hua, Rui-Xi; Du, Yi-Qun; Huang, Ming-Zhu; Liu, Yong; Cheng, Yu Fang; Guo, Wei-Jian 2016-09-27 Mel-18, a polycomb group protein, has been reported to act as a tumor suppressor and be down-regulated in several human cancers including gastric cancer. It was also found that Mel-18 negatively regulates self-renewal of hematopoietic stem cells and breast cancer stem cells (CSCs). This study aimed to clarify its role in gastric CSCs and explore the mechanisms. We found that low-expression of Mel-18 was correlated with poor prognosis and negatively correlated with overexpression of stem cell markers Oct4, Sox2, and Gli1 in 101 gastric cancer tissues. Mel-18 was down-regulated in cultured spheroid cells, which possess CSCs, and overexpression of Mel-18 inhibits cells sphere-forming ability and tumor growth in vivo. Besides, Mel-18 was lower-expressed in ovary metastatic lesions compared with that in primary lesions of gastric cancer, and Mel-18 overexpression inhibited the migration ability of gastric cancer cells. Interestingly, overexpression of Mel-18 resulted in down-regulation of miR-21 in gastric cancer cells and the expression of Mel-18 was negatively correlated with the expression of miR-21 in gastric cancer tissues. Furthermore, miR-21 overexpression partially restored sphere-forming ability, migration potential and chemo-resistance in Mel-18 overexpressing gastric cancer cells. These results suggests Mel-18 negatively regulates stem cell-like properties through downregulation of miR-21 in gastric cancer cells. 54. Ultrasound guided percutaneous treatment for splenic abscesses: The significance in treatment of critically ill patients Institute of Scientific and Technical Information of China (English) Enver Zerem; Jacob Bergsland 2006-01-01 AIM: To analyze the results of ultrasound guided percutaneous needle aspiration (PNA) and percutaneous catheter drainage (PCD) in the treatment of splenic abscess.METHODS: Thirty-six patients (14 females and 22 males, with an average age of 54.1 ± 14.1 years) with splenic abscess were treated with ultrasound guided PNA and/or PCD. Patients with splenic abscess ＜ 50 mm in diameter were initially treated by PNA and those with abscess ≥ 50 mm and bilocular abscesses were initially treated by an 8-French catheter drainage. The clinical characteristics, underlying diseases, organism spectra,therapeutic methods, and morality rates were analyzed.RESULTS: Twenty-seven patients had unilocular and 9 bilocular abscess. PNA was performed in 19 patients (52.8%), and 8 of them (42.1%) required PCD because of recurrence of abscess. In 17 patients (47.2%), PCD was performed initially. PCD was performed twice in six patients and three times in two. PNA was definitive treatment for 10 and PCD for 21 patients. One patient with PCD was referred for splenectomy, with successful outcome. In all 4 deceased patients, malignancy was the underlying condition. Twenty-one patients (58.3%)underwent 33 surgical interventions on abdomen before treatment. Cultures were positive in 30 patients (83.3%).Gram-negative bacillus predominated (46.7%). There were no complications related to the procedure.CONCLUSION: Percutaneous treatmnet of splenic abscess is an effective alternative to surgery, allowing preservation of the spleen. This treatment is especially indicative for the patients in critical condition postoperatively. We recommend PNA as primary treatment for splenic abscesses ＜ 50 mm, and PCD for those ≥ 50 mm in diameter and for bilocular abscesses. 55. Nature's knockout: the Mel1b receptor is not necessary for reproductive and circadian responses to melatonin in Siberian hamsters. Science.gov (United States) Weaver, D R; Liu, C; Reppert, S M 1996-11-01 The pineal hormone melatonin regulates seasonal reproduction and influences the timing of circadian rhythms. The Mel1a and Mel1b receptors are the high-affinity melatonin receptors present in mammals. Unexpectedly, the Mel1b receptor gene of the Siberian hamster, Phodopus sungorus, cannot encode a functional receptor; two nonsense mutations are present within the coding region. Southern blot analysis indicates that this is a single copy gene. The Mel1b receptor gene is nonfunctional in outbred populations of P. sungorus and Phodopus campbelli. Siberian hamsters lacking a functional Mel1b receptor nevertheless show seasonal reproductive and circadian responses to melatonin, indicating that the Mel1b receptor is not necessary for these responses. These data support the hypothesis that the Mel1a receptor, which does encode a functional receptor in this species, mediates reproductive and circadian responses to melatonin. 56. Léon Lederman, Mel Schwartz and Jack Steinberger wre awarded the 1988 Nobel Physics Prize. CERN Multimedia Photographic Service 1988-01-01 Léon Lederman (left), Mel Schwartz (right) and Jack Steinberger were awarded the 1988 Nobel Physics Prize for their 1962 experiment at Brookhaven which showed that neutrinos come in more than one kind. 57. Thermal conductivity of pure silica MEL and MFI zeolite thin films Science.gov (United States) Coquil, Thomas; Lew, Christopher M.; Yan, Yushan; Pilon, Laurent 2010-08-01 This paper reports the room temperature cross-plane thermal conductivity of pure silica zeolite (PSZ) MEL and MFI thin films. PSZ MEL thin films were prepared by spin coating a suspension of MEL nanoparticles in 1-butanol solution onto silicon substrates followed by calcination and vapor-phase silylation with trimethylchlorosilane. The mass fraction of nanoparticles within the suspension varied from 16% to 55%. This was achieved by varying the crystallization time of the suspension. The thin films consisted of crystalline MEL nanoparticles embedded in a nonuniform and highly porous silica matrix. They featured porosity, relative crystallinity, and MEL nanoparticles size ranging from 40% to 59%, 23% to 47% and 55 nm to 80 nm, respectively. PSZ MFI thin films were made by in situ crystallization, were b-oriented, fully crystalline, and had a 33% porosity. Thermal conductivity of these PSZ thin films was measured at room temperature using the 3ω method. The cross-plane thermal conductivity of the MEL thin films remained nearly unchanged around 1.02±0.10 W m-1 K-1 despite increases in (i) relative crystallinity, (ii) MEL nanoparticle size, and (iii) yield caused by longer nanoparticle crystallization time. Indeed, the effects of these parameters on the thermal conductivity were compensated by the simultaneous increase in porosity. PSZ MFI thin films were found to have similar thermal conductivity as MEL thin films even though they had smaller porosity. Finally, the average thermal conductivity of the PSZ films was three to five times larger than that reported for amorphous solgel mesoporous silica thin films with similar porosity and dielectric constant. 58. CaMELS: In silico prediction of calmodulin binding proteins and their binding sites. Science.gov (United States) Abbasi, Wajid Arshad; Asif, Amina; Andleeb, Saiqa; Minhas, Fayyaz Ul Amir Afsar 2017-09-01 Due to Ca(2+) -dependent binding and the sequence diversity of Calmodulin (CaM) binding proteins, identifying CaM interactions and binding sites in the wet-lab is tedious and costly. Therefore, computational methods for this purpose are crucial to the design of such wet-lab experiments. We present an algorithm suite called CaMELS (CalModulin intEraction Learning System) for predicting proteins that interact with CaM as well as their binding sites using sequence information alone. CaMELS offers state of the art accuracy for both CaM interaction and binding site prediction and can aid biologists in studying CaM binding proteins. For CaM interaction prediction, CaMELS uses protein sequence features coupled with a large-margin classifier. CaMELS models the binding site prediction problem using multiple instance machine learning with a custom optimization algorithm which allows more effective learning over imprecisely annotated CaM-binding sites during training. CaMELS has been extensively benchmarked using a variety of data sets, mutagenic studies, proteome-wide Gene Ontology enrichment analyses and protein structures. Our experiments indicate that CaMELS outperforms simple motif-based search and other existing methods for interaction and binding site prediction. We have also found that the whole sequence of a protein, rather than just its binding site, is important for predicting its interaction with CaM. Using the machine learning model in CaMELS, we have identified important features of protein sequences for CaM interaction prediction as well as characteristic amino acid sub-sequences and their relative position for identifying CaM binding sites. Python code for training and evaluating CaMELS together with a webserver implementation is available at the URL: http://faculty.pieas.edu.pk/fayyaz/software.html#camels. © 2017 Wiley Periodicals, Inc. 59. The Involvement of a Concerned Significant Other in Gambling Disorder Treatment Outcome. Science.gov (United States) Jiménez-Murcia, Susana; Tremblay, Joël; Stinchfield, Randy; Granero, Roser; Fernández-Aranda, Fernando; Mestre-Bach, Gemma; Steward, Trevor; Del Pino-Gutiérrez, Amparo; Baño, Marta; Moragas, Laura; AymamÃ, Neus; Gómez-Peña, Mónica; Tárrega, Salomé; Valenciano-Mendoza, Eduardo; Giroux, Isabelle; Sancho, Marta; Sánchez, Isabel; MallorquÃ-Bagué, Núria; González, Vega; MartÃn-Romera, Virginia; Menchón, José M 2016-11-17 Interpersonal distress is a common feature in gambling disorder and adding a concerned significant other (CSO) to the recovery process could be an effective tool for improving treatment outcome. However, little empirical evidence is available regarding the effectiveness of including a CSO to interventions. We aimed to compare treatment outcomes (i.e. compliance with therapy guidelines, dropout from treatment, and relapse during treatment) in a CBT program involving a CSO to CBT treatment as usual (TAU) without a CSO. The sample comprised male gambling disorder patients (N = 675). The manualized CBT intervention consisted of 16 weekly outpatient group sessions and a 3-month follow-up period. Patient CSOs attended a predetermined number of sessions with the patient and were provided with resources to acquire a better understanding of the disorder, to manage risk situations, and to aid patients in adhering to treatment guidelines. Patients with a CSO had significant higher treatment attendance and reduced dropout compared to patients receiving TAU. Moreover, patients whose spouse was involved in the treatment program were less likely to relapse and adhered to the treatment guidelines more than those with a non-spousal CSO. Our results suggest that incorporating interpersonal support to gambling disorder interventions could potentially improve treatment outcomes. 60. Significant anti-tumor effect of bevacizumab in treatment of pineal gland glioblastoma multiforme. Science.gov (United States) Mansour, Joshua; Fields, Braxton; Macomson, Samuel; Rixe, Olivier 2014-12-01 Glioblastoma multiforme (GBM) is the most aggressive subtype of malignant gliomas. Current standard treatment for GBM involves a combination of cytoreduction through surgical resection, followed by radiation with concomitant and adjuvant chemotherapy (temozolomide). The role of bevacizumab in the treatment of GBM continues to be a topic of ongoing research and debate. Despite aggressive treatment, these tumors remain undoubtedly fatal, especially in the elderly. Furthermore, tumors present in the pineal gland are extremely rare, accounting for only 0.1-0.4 % of all adult brain tumors, with this location adding to the complexity of treatment. We present a case of GBM, at the rare location of pineal gland, in an elderly patient who was refractory to initial standard of care treatment with radiation and concomitant and adjuvant temozolomide, but who developed a significant response to

anti-angiogenic therapy using bevacizumab. « 1 2 3 4 5 » « 2 3 4 5 6 » 61. Classificação do mel em floral ou mel de melato Classification of honey as floral or honeydew honey Directory of Open Access Journals (Sweden) G. Campos 2003-04-01 Full Text Available O mel é um produto natural de abelhas fabricado a partir do néctar das flores (mel floral e de secreções de partes vivas das plantas, ou de excreções de insetos sugadores de partes vivas das plantas (mel de melato. O melato é uma fonte alternativa de alimento bastante utilizada pela abelha. Em Santa Catarina, o mel de melato é muito conhecido, sendo produzido nos meses de janeiro a abril, em ciclos bianuais. Foram colhidas aleatoriamente 25 amostras de mel, no perÃodo de janeiro/93 a junho/96, de diferentes apiários e datas de colheita. Foram determinados os teores de umidade, pH, cinzas e açúcares redutores. Os resultados foram utilizados na aplicação da equaçà £o de Kirkwood para classificar o mel como floral ou de melato. As amostras também foram analisadas quanto à rotação óptica para classificação de acordo com White. Das 25 amostras analisadas, 11 foram classificadas como mel de melato de acordo com Kirkwood e White. O estudo estatÃstico, feito pela aplicação do teste exato de Fisher, indicou que, apesar da divergência em 4 amostras, estes métodos são equivalentes e ambos podem ser usados.Honey is a natural product made by bees using flower's nectar (floral honey or excretion of alive parts of plants or either excretion produced by sucking insects from alive parts of plants (honeydew honey. Honeydew is an alternative food source used by bees. In Santa Catarina state, honeydew honey is produced from january to april, each two years. In order to identify the presence of honeydew honey, 25 samples were sampled randomly from january/93 to june/96. They were colected from different apiaries in different periods of time and tested for moisture, pH, ash, reducing sugars, and the results were used in the application of Kirkwood equation to classify honey as floral or honeydew. They were analised for polarimetry, to classify them according to Kirkwood and White. The statistical study was done by the application of exact 62. The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations. Science.gov (United States) Walker, T; Johnson, P H; Moreira, L A; Iturbe-Ormaetxe, I; Frentiu, F D; McMeniman, C J; Leong, Y S; Dong, Y; Axford, J; Kriesner, P; Lloyd, A L; Ritchie, S A; O'Neill, S L; Hoffmann, A A 2011-08-24 Dengue fever is the most important mosquito-borne viral disease of humans with more than 50 million cases estimated annually in more than 100 countries. Disturbingly, the geographic range of dengue is currently expanding and the severity of outbreaks is increasing. Control options for dengue are very limited and currently focus on reducing population abundance of the major mosquito vector, Aedes aegypti. These strategies are failing to reduce dengue incidence in tropical communities and there is an urgent need for effective alternatives. It has been proposed that endosymbiotic bacterial Wolbachia infections of insects might be used in novel strategies for dengue control. For example, the wMelPop-CLA Wolbachia strain reduces the lifespan of adult A. aegypti mosquitoes in stably transinfected lines. This life-shortening phenotype was predicted to reduce the potential for dengue transmission. The recent discovery that several Wolbachia infections, including wMelPop-CLA, can also directly influence the susceptibility of insects to infection with a range of insect and human pathogens has markedly changed the potential for Wolbachia infections to control human diseases. Here we describe the successful transinfection of A. aegypti with the avirulent wMel strain of Wolbachia, which induces the reproductive phenotype cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, providing optimal phenotypic effects for invasion. Under semi-field conditions, the wMel strain increased from an initial starting frequency of 0.65 to near fixation within a few generations, invading A. aegypti populations at an accelerated rate relative to trials with the wMelPop-CLA strain. We also show that wMel and wMelPop-CLA strains block transmission of dengue serotype 2 (DENV-2) in A. aegypti, forming the basis of a practical approach to dengue suppression. 63. Characterization of the melA Locus for α-Galactosidase in Lactobacillus plantarum Science.gov (United States) Silvestroni, Aurelio; Connes, Cristelle; Sesma, Fernando; Savoy de Giori, Graciela; Piard, Jean-Christophe 2002-01-01 Alpha-galactosides are abundant sugars in legumes such as soy. Because of the lack of α-galactosidase (α-Gal) in the digestive tract, humans are unable to digest these sugars, which consequently induce flatulence. To develop the consumption of the otherwise highly nutritional soy products, the use of exogenous α-Gal is promising. In this framework, we characterized the melA gene for α-Gal in Lactobacillus plantarum. The melA gene encodes a cytoplasmic 84-kDa protein whose enzymatically active form occurs as oligomers. The melA gene was cloned and expressed in Escherichia coli, yielding an active α-Gal. We show that melA is transcribed from its own promoter, yielding a monocistronic mRNA, and that it is regulated at the transcriptional level, i.e., it is induced by melibiose but is not totally repressed by glucose. Posttranscriptional regulation by the carbon source could also occur. Upstream of melA, a putative galactoside transporter, designated RafP, was identified that shows high homology to LacS, the unique transporter for both α- and βgalactosides in Streptococcus thermophilus. rafP is also expressed as a monocistronic mRNA. Downstream of melA, the lacL and lacM genes were identified that encode a heterodimeric β-galactosidase. A putative galM gene identified in the same cluster suggests the presence of a galactose operon. These results indicate that the genes involved in galactoside catabolism are clustered in L. plantarum ATCC 8014. This first genetic characterization of melA and of its putative associated transporter, rafP, in a lactobacillus opens doors to various applications both in the manufacture of soy-derived products and in probiotic and nutraceutical issues. PMID:12406739 64. Metagenomic analysis reveals significant changes of microbial compositions and protective functions during drinking water treatment. Science.gov (United States) Chao, Yuanqing; Ma, Liping; Yang, Ying; Ju, Feng; Zhang, Xu-Xiang; Wu, Wei-Min; Zhang, Tong 2013-12-19 The metagenomic approach was applied to characterize variations of microbial structure and functions in raw (RW) and treated water (TW) in a drinking water treatment plant (DWTP) at Pearl River Delta, China. Microbial structure was significantly influenced by the treatment processes, shifting from Gammaproteobacteria and Betaproteobacteria in RW to Alphaproteobacteria in TW. Further functional analysis indicated the basic metabolic functions of microorganisms in TW did not vary considerably. However, protective functions, i.e. glutathione synthesis genes in 'oxidative stress' and 'detoxification' subsystems, significantly increased, revealing the surviving bacteria may have higher chlorine resistance. Similar results were also found in glutathione metabolism pathway, which identified the major reaction for glutathione synthesis and supported more genes for glutathione metabolism existed in TW. This metagenomic study largely enhanced our knowledge about the influences of treatment processes, especially chlorination, on bacterial community structure and protective functions (e.g. glutathione metabolism) in ecosystems of DWTPs. 65. Complementary Therapies for Significant Dysfunction from Tinnitus: Treatment Review and Potential for Integrative Medicine Directory of Open Access Journals (Sweden) Ruth Q. Wolever 2015-01-01 Full Text Available Tinnitus is a prevalent and costly chronic condition; no universally effective treatment exists. Only 20% of patients who report tinnitus actually seek treatment, and when treated, most patients commonly receive sound-based and educational (SBE therapy. Additional treatment options are necessary, however, for nonauditory aspects of tinnitus (e.g., anxiety, depression, and significant interference with daily life and when SBE therapy is inefficacious or inappropriate. This paper provides a comprehensive review of (1 conventional tinnitus treatments and (2 promising complementary therapies that have demonstrated some benefit for severe dysfunction from tinnitus. While there has been no systematic study of the benefits of an Integrative Medicine approach for severe tinnitus, the current paper reviews emerging evidence suggesting that synergistic combinations of complementary therapies provided within a whole-person framework may augment SBE therapy and empower patients to exert control over their tinnitus symptoms without the use of medications, expensive devices, or extended programs. 66. Serum ionic fluoride concentrations are significantly decreased after treatment with alendronate in patients with osteoporosis. Science.gov (United States) Sato, Hironori; Tanno, Kozo; Muro-oka, Genyoh; Itai, Kazuyoshi 2011-11-20 We determined serum ionic fluoride (SIF) concentrations before and after treatment of osteoporosis with alendronate to clarify whether SIF concentrations directly reflect a change in bone metabolism. A total of 45 postmenopausal women with primary osteoporosis who were treated with alendronate over a 6-month period were enrolled (mean age, 64.2 years). SIF concentrations were measured by the flow injection method with an ion-selective electrode. Concentrations of bone turnover markers (serum bone alkaline phosphatase, serum osteocalcin, serum type I collagen cross-linked N-telopeptide and urinary deoxypryridinoline) and lumbar spine BMD (LsBMD) were also measured. SIF, bone turnover markers and LsBMD before and after treatment were compared. Concentrations of SIF as well as concentrations of all bone turnover markers were significantly decreased after treatment: means (standard deviations) before and after treatment were 0.62 (0.13) and 0.32 (0.09) μmol/l, respectively (P 50 days but were much higher in stage III patients with OTT < 50 than OTT> 50 days ( P < 0.001. It was also observed that patients who received BED 2.5 < 105 had lesser rectal ( P < 0.001 and bladder complications than BED 2.5 > 105. Higher rectal complication-free survival (CFS R rate, bladder complication-free survival (CFS B rate and all-type late complication-free survival rate were observed in patients who received BED 2.5 < 105 than BED 2.5 > 105. A balanced, optimal and justified radiotherapy treatment schedule to deliver higher BED 10 (>84.5 and lower BED 2.5 (< 105 in lesser OTT (< 50 days is essential in carcinoma cervix to expect a better treatment outcome in all respects. 116. [Fosfomycin--its significance for treatment of diseases due to multidrug-resistant bacteria]. Science.gov (United States) Stock, Ingo 2015-01-01 Fosfomycin is a bactericidal phosphonic acid derivative, which engages by inhibiting pyruvyltransferase at an early stage in the peptidoglycan synthesis. It shows a broad spectrum of activity that includes many multidrug-resistant gram-negative and gram-positive bacteria. Fosfomycin is active against most strains of Pseudomonas aeruginosa and several multidrugresistant Enterobacteriaceae, e.g., Escherichia coli strains expressing extended spectrum beta-lactamases (ESBL) and Klebsiella pneumoniae strains with decreased susceptibilities to carbapenems. Most methicillin-resistant Staphylococcus aureus (MRSA) strains as well as enterococci with and without vancomycin resistance are also sensitive to fosfomycin. During the last decade, a variety of studies showed that fosfomycin is not only suitable for treating uncomplicated urinary tract diseases, but also for the treatment of many other diseases caused by bacterial pathogens with and without multidrug resistance. However, large controlled studies demonstrating the efficacy of the drug to treat diseases caused by multidrug-resistant bacteria are still missing. Considering the low number of antibacterial agents with good activity against multidrug-resistant bacteria, fosfomycin should be evaluated as an important antibiotic for the treatment of several severe illnesses due to these pathogens. However, because some multidrug-resistant bacteria are also resistant to fosfomycin, this agent should only be applied if the pathogen is sensitive to this drug. In addition, because rapid development of resistance cannot be excluded if fosfomycin will be applied alone, this drug should only be given in combination with other effective drugs for the treatment of serious systemic diseases due to multidrug-resistant bacterial pathogens. 117. Corticortophin releasing factor 2 receptor agonist treatment significantly slows disease progression in mdx mice Directory of Open Access Journals (Sweden) Stevens Paula J 2007-07-01 Full Text Available Abstract Background Duchenne muscular dystrophy results from mutation of the dystrophin gene, causing skeletal and cardiac muscle loss of function. The mdx mouse model of Duchenne muscular dystrophy is widely utilized to evaluate the potential of therapeutic regimens to modulate the loss of skeletal muscle function associated with dystrophin mutation. Importantly, progressive loss of diaphragm function is the most consistent striated muscle effect observed in the mdx mouse model, which is the same as in patients suffering from Duchenne muscular dystrophy. Methods Using the mdx mouse model, we have evaluated the effect that corticotrophin releasing factor 2 receptor (CRF2R agonist treatment has on diaphragm function, morphology and gene expression. Results We have observed that treatment with the potent CRF2R-selective agonist PG-873637 prevents the progressive loss of diaphragm specific force observed during aging of mdx mice. In addition, the combination of PG-873637 with glucocorticoids not only prevents the loss of diaphragm specific force over time, but also results in recovery of specific force. Pathological analysis of CRF2R agonist-treated diaphragm muscle demonstrates that treatment reduces fibrosis, immune cell infiltration, and muscle architectural disruption. Gene expression analysis of CRF2R-treated diaphragm muscle showed multiple gene expression changes including globally decreased immune cell-related gene expression, decreased extracellular matrix gene expression, increased metabolism-related gene expression, and, surprisingly, modulation of circadian rhythm gene expression. Conclusion Together, these data demonstrate that CRF2R activation can prevent the progressive degeneration of

diaphragm muscle associated with dystrophin gene mutation. 118. The significance of HPV in the follow-up period after treatment for CIN. Science.gov (United States) Gallwas, J; Ditsch, N; Hillemanns, P; Friese, K; Thaler, C; Dannecker, C 2010-01-01 High-risk anogenital human papillomavirus (HPV) infections are causally related to cervical cancer. Successful treatment of cervical intraepithelial neoplasia (CIN) results in complete eradication of HPV in most cases. There is an increasing interest regarding the role of HPV testing in the follow-up period after treatment for CIN. This retrospective study includes 107 women who underwent conization for histologically verified CIN. All of them had HPV testing pre- and postoperatively. HPV testing was carried out using a hybrid capture assay (HC2). The mean follow-up period was 21.4 months (range 2-76 months). The data were analyzed with respect to success of conization, HPV persistence/recurrence and CIN recurrence. Sensitivity, specificity and negative predictive value (NPV) of HPV testing were assessed and compared to the cytological results. Preoperatively, 97 of 107 women were HPV positive. Ninety-seven conizations showed negative resection margins with 86 women becoming HPV negative. In the following months, nine of these HPV negative women became HPV positive again. Out of ten conizations with positive resection margins, six women became HPV negative. Recurrent CIN 2/3 lesions were observed in 11 women, nine of whom had persistent positive HPV testing throughout the entire study period. Regarding CIN recurrence HPV testing showed a sensitivity of 93%, a specificity of 85% and a NPV of 99%. The sensitivity of HPV testing concerning persistent or recurrent CIN as well as the NPV are high. The present data suggest that HPV testing should be integrated in a follow-up algorithm after treatment for CIN by conization. 119. Communication: Proper treatment of classically forbidden electronic transitions significantly improves detailed balance in surface hopping. Science.gov (United States) Sifain, Andrew E; Wang, Linjun; Prezhdo, Oleg V 2016-06-01 Surface hopping is the most popular method for nonadiabatic molecular dynamics. Many have reported that it does not rigorously attain detailed balance at thermal equilibrium, but does so approximately. We show that convergence to the Boltzmann populations is significantly improved when the nuclear velocity is reversed after a classically forbidden hop. The proposed prescription significantly reduces the total number of classically forbidden hops encountered along a trajectory, suggesting that some randomization in nuclear velocity is needed when classically forbidden hops constitute a large fraction of attempted hops. Our results are verified computationally using two- and three-level quantum subsystems, coupled to a classical bath undergoing Langevin dynamics. 120. Diagnosis and treatment of spontaneous coronary artery pseudoaneurysm: Rare anomaly with potentially significant clinical implications. Science.gov (United States) Kar, Subrata; Webel, Richard R 2017-03-04 Spontaneous coronary artery pseudoaneurysm (PSA, false aneurysm) is an extremely rare occurrence with the precise incidence unknown. It is defined as an outwardly bulging monolayer or double layer within the coronary artery that lacks all 3 layers (intima, media, and adventitia) of the arterial wall. Coronary PSA commonly occurs from arterial dissection or perforation induced by catheter intervention, infection, pregnancy, or trauma. Traumatic dissection or perforation of the coronary artery after a percutaneous coronary intervention (PCI) remains the most common cause. Such cases may progress to myocardial ischemia, acute myocardial infarction, or acute coronary artery rupture causing death from cardiac tamponade. Intravascular ultrasound or cardiac computed tomography may aid in the diagnosis. Treatment options include PCI with a covered stent, bare or drug-eluting stent, coil embolization, coronary artery bypass graft with isolation of the PSA, or conservative management with vigilant clinical follow-up. In this review, we sought to describe the diagnosis, etiology, treatment, and the limited literature on spontaneous coronary artery PSA. © 2017 Wiley Periodicals, Inc. « 4 5 6 7 8 » « 5 6 7 8 9 » 121. Significance of Kampo, Japanese Traditional Medicine, in the Treatment of Obesity: Basic and Clinical Evidence Directory of Open Access Journals (Sweden) Jun-ichi Yamakawa 2013-01-01 Full Text Available The cause of obesity includes genetic and environmental factors, including cytokines derived from adipocytes (adipo-cytokines. Although drug therapy is available for obesity, it is highly risky. Our main focus in this review is on the traditional form of Japanese medicine, Kampo, in the treated of obesity. Two Kampo formulas, that is, bofutsushosan (防風通è–æ•£ and boiogito (防己黄耆湯, are covered by the national health insurance in Japan for the treatment of obesity. Various issues related to their action mechanisms remain unsolved. Considering these, we described the results of basic experiments and presented clinical evidence and case reports on osteoarthritis as examples of clinical application of their two Kampo medicine. Traditional medicine is used not only for treatment but also for prevention. In clinical practice, it is of great importance to prove the efficacy of combinations of traditional medicine and Western medicine and the utility of traditional medicine in the attenuation of adverse effects of Western medicine. 122. Significance of serum 25-hydroxyvitamin D3 and interleukin-6 levels in immunoglobulin treatment of Kawasaki disease in children. Science.gov (United States) An, Xinjiang; Fu, Mingyu; Tian, Jing; Xue, Ying; Xu, Hui 2016-09-01 The aim of the study was to investigate the significance of the level of serum 25-hydroxyvitamin D3 [25-(OH)D3] and interleukin (IL)-6 in serum prior to and after immunoglobulin treatment in children suffering from Kawasaki disease in order to provide a reference for the successful treatment of Kawasaki disease in children. From February, 2013 to February, 2015, 45 patients with Kawasaki disease were enrolled in the observation group. The normal control group comprised 43 healthy volunteers and the feverish control group 46 patients with respiratory infection and fever. Venous blood was collected from each case before and after immunoglobulin treatment and the level of 25-(OH)D3 and IL-6 in the serum were measured using fluorescent quantitative PCR, enzyme-linked immunosorbent assay and western blotting. Before treatment, the level of 25-(OH)D3 in the feverish control group was significantly lower than that of the normal control group, while the level of 25-(OH)D3 in the observation group was significantly higher than that of the normal control group. The level of 25-(OH)D3 in the feverish control group was lower than the IL-6 level in the normal children, but the difference was not statistically significant (P>0.05). The level 25-(OH)D3 in the observation group was significantly higher than the IL-6 level in the normal control group. The serum content of 25-(OH)D3 was significantly higher after the treatment compared to before treatment levels and after treatment IL-6 level was only slightly lower. It was observed that the 25-(OH)D3 level in the observation group was significantly increased after immunoglobulin treatment and this was positively correlated with the effects of the treatment. The IL-6 level had no significant changes after treatment and had little correlation with the treatment effect. The results suggested that 25-(OH)D3 may be involved in the occurrence of Kawasaki disease in children and in the aggravation of the disease to some extent. 123. Clinically Significant Change to Establish Benchmarks in Residential Drug and Alcohol Treatment Services Science.gov (United States) Billingham, Daniel D.; Kelly, Peter J.; Deane, Frank P.; Crowe, Trevor P.; Buckingham, Mark S.; Craig, Fiona L. 2012-01-01 There is increasing emphasis on the use routine outcome assessment measures to inform quality assurance initiatives. The calculation of reliable and clinically significant change indices is one strategy that organizations could use to develop both internal and externally focused benchmarking processes. The current study aimed to develop reliable… 124. The wMel Strain of Wolbachia Reduces Transmission of Chikungunya Virus in Aedes aegypti. Directory of Open Access Journals (Sweden) Matthew T Aliota 2016-04-01 Full Text Available New approaches to preventing chikungunya virus (CHIKV are needed because current methods are limited to controlling mosquito populations, and they have not prevented the invasion of this virus into new locales, nor have they been sufficient to control the virus upon arrival. A promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium Wolbachia into Aedes aegypti mosquitoes. This primarily has been proposed as a tool to control dengue virus (DENV transmission; however, evidence suggests Wolbachia infections confer protection for Ae. aegypti against CHIKV. Although this approach holds much promise for limiting virus transmission, at present our understanding of the ability of CHIKV to infect, disseminate, and be transmitted by wMel-infected Ae. aegypti currently being used at Wolbachia release sites is limited.Using Ae. aegypti infected with the wMel strain of Wolbachia that are being released in Medellin, Colombia, we report that these mosquitoes have reduced vector competence for CHIKV, even with extremely high viral titers in the bloodmeal. In addition, we examined the dynamics of CHIKV infection over the course of four to seven days post feeding. Wolbachia-infected mosquitoes remained non-infective over the duration of seven days, i.e., no infectious virus was detected in the saliva when exposed to bloodmeals of moderate viremia, but CHIKV-exposed, wild type mosquitoes did have viral loads in the saliva consistent with what has been reported elsewhere. Finally, the presence of wMel infection had no impact on the lifespan of mosquitoes as compared to wild type mosquitoes following CHIKV infection.These results could have an impact on vector control strategies in areas where Ae. aegypti are transmitting both DENV and CHIKV; i.e., they argue for further exploration, both in the laboratory and the field, on the feasibility of expanding this technology beyond DENV. 125. Membrane bioreactor wastewater treatment plants reveal diverse yeast and protist communities of potential significance in biofouling. Science.gov (United States) Liébana, Raquel; Arregui, LucÃa; Belda, Ignacio; Gamella, Luis; Santos, Antonio; Marquina, Domingo; Serrano, Susana 2015-01-01 The yeast community was studied in a municipal full-scale membrane bioreactor wastewater treatment plant (MBR-WWTP). The unexpectedly high diversity of yeasts indicated that the activated sludge formed a suitable environment for them to proliferate, with cellular concentrations of 2.2 ± 0.8 × 10(3) CFU ml(-1). Sixteen species of seven genera were present in the biological reactor, with Ascomycetes being the most prevalent group (93%). Most isolates were able to grow in a synthetic wastewater medium, adhere to polyethylene surfaces, and develop biofilms of variable complexity. The relationship between yeast populations and the protists in the MBR-WWTP was also studied, revealing that some protist species preyed on and ingested yeasts. These results suggest that yeast populations may play a role in the food web of a WWTP and, to some extent, contribute to membrane biofouling in MBR systems. 126. No gender differences in prognosis and preventive treatment in patients with AMI without significant stenoses DEFF Research Database (Denmark) Hansen, Kim Wadt; Hvelplund, Anders; Abildstrøm, Steen Zabell 2012-01-01 Objective: To investigate possible gender differences in patients with acute myocardial infarction (AMI) and without significant stenoses on coronary angiography (CAG) regarding prognosis and use of secondary preventive medication. Design: Nationwide register-based cohort study. Patients: By comp......Objective: To investigate possible gender differences in patients with acute myocardial infarction (AMI) and without significant stenoses on coronary angiography (CAG) regarding prognosis and use of secondary preventive medication. Design: Nationwide register-based cohort study. Patients...... a prescription for a lipid-lowering drug, beta-blocker, clopidogrel, or aspirin within 60 days of discharge. Results: During follow-up, 97 women and 60 men died, resulting in a crude female/male hazard ratio (HR) of 1.51 (95% CI 1.09-2.08). After adjustment for age, time-period, and comorbidity, the gender... 127. Mel Frequency Cepstral Coefficients: An Evaluation of Robustness of MP3 Encoded Music DEFF Research Database (Denmark) Sigurdsson, Sigurdur; Petersen, Kaare Brandt; Lehn-Schiøler, Tue 2006-01-01 In large MP3 databases, files are typically generated with different parameter settings, i.e., bit rate and sampling rates. This is of concern for MIR applications, as encoding difference can potentially confound meta-data estimation and similarity evaluation. In this paper we will discuss...... the influence of MP3 coding for the Mel frequency cepstral coeficients (MFCCs). The main result is that the widely used subset of the MFCCs is robust at bit rates equal or higher than 128 kbits/s, for the implementations we have investigated. However, for lower bit rates, e.g., 64 kbits/s, the implementation... 128. Analysis and prediction of acoustic speech features from mel-frequency cepstral coefficients in distributed speech recognition architectures. Science.gov (United States) Darch, Jonathan; Milner, Ben; Vaseghi, Saeed 2008-12-01 The aim of this work is to develop methods that enable acoustic speech features to be predicted from mel-frequency cepstral coefficient (MFCC) vectors as may be encountered in distributed speech recognition architectures. The work begins with a detailed analysis of the multiple correlation between acoustic speech features and MFCC vectors. This confirms the existence of correlation, which is found to be higher when measured within specific phonemes rather than globally across all speech sounds. The correlation analysis leads to the development of a statistical method of predicting acoustic speech features from MFCC vectors that utilizes a network of hidden Markov models (HMMs) to localize prediction to specific phonemes. Within each HMM, the joint density of acoustic features and MFCC vectors is modeled and used to make a maximum a posteriori prediction. Experimental results are presented across a range of conditions, such as with speaker-dependent, gender-dependent, and gender-independent constraints, and these show that acoustic speech features can be predicted from MFCC vectors with good accuracy. A comparison is also made against an alternative scheme that substitutes the higher-order MFCCs with acoustic features for transmission. This delivers accurate acoustic features but at the expense of a significant reduction in speech recognition accuracy. 129. Increasing significance of advanced physical/chemical processes in the development and application of sustainable wastewater treatment systems Institute of Scientific and Technical Information of China (English) Wim RULKENS 2008-01-01 The awareness of the problem of the scarcity of water of high quality has strongly changed the approach of wastewater treatment. Currently, there is an increasing need for the beneficial reuse of treated wastewater and to recover valuable products and energy from the wastewater. Because microbiological treatment methods are, only to a limited part, able to satisfy these needs, the role and significance of physical/chemical pro-cesses in wastewater treatment are gaining more and more interest. The specific future role and aim of the various physical/chemical treatment processes can be categorized in five groups: improvement of the perform-ance of microbiological treatment processes, achieve-ment of the high quality required for reuse of the effluent, recovery of valuable components and energy from the wastewater for beneficial reuse, desalination of brackish water and seawater, and treatment of con-centrated liquid or solid waste residues produced in a wastewater treatment process. Development of more environmentally sustainable wastewater treatment chains in which physical/chemical processes play a cru-cial role, also requires application of process control and modeling strategies. This is briefly introduced by the elaboration of treatment scenarios for three specific wastewaters. 130. Immune disorders in sepsis and their treatment as a significant problem of modern intensive care. Science.gov (United States) Åysenko, Lidia; LeÅ›nik, Patrycja; Nelke, Kamil; Gerber, Hanna 2017-08-22 Despite the great advances in the treatment of sepsis over the past 20 years, sepsis remains the main cause of death in intensive care units. In the context of new possibilities of treating sepsis, a comprehensive response of the immune system to the infection, immunosuppression, in particular, has in recent years gained considerable interest. There is vast evidence pointing to the correlation between comorbid immunosuppression and an increased risk of recurrent infections and death. Immune disorders may impact the clinical course of sepsis. This applies in particular to patients with deteriorated clinical response to infections. They usually suffer from comorbidities and conditions accompanied by immunosuppression. Sepsis disrupts innate and adaptive immunity. The key to diagnose the immune disorders in sepsis and undertake targeted immunomodulatory therapy is to define the right biomarkers and laboratory methods, which permit prompt "bedside" diagnosis. Flow cytometry is a laboratory tool that meets these criteria. Two therapeutic methods are currently being suggested to restore the immune homeostasis of sepsis patients. Excessive inflammatory response may be controlled through extracorporeal blood purification techniques, in large part derived from renal replacement therapy. These are such techniques as high-volume haemofiltration, cascade haemofiltration, plasma exchange, coupled plasma filtration and adsorption, high-absorption membranes, high cut-off membranes. The main task of theses techniques is the selective elimination of middle molecular weight molecules, such as cytokines. Pharmacotherapy with the use of such immunostimulants as interleukin 7, granulocyte-macrophage colony-stimulating factor, interferon gamma, PD-1, PD-L1 and CTLA-4 antagonists, intravenous immunoglobulins may help fight immunosuppressive immune disorders. 131. Significant improvement of pig cloning efficiency by treatment with LBH589 after somatic cell nuclear transfer. Science.gov (United States) Jin, Jun-Xue; Li, Suo; Gao, Qing-Shan; Hong, Yu; Jin, Long; Zhu, Hai-Ying; Yan, Chang-Guo; Kang, Jin-Dan; Yin, Xi-Jun 2013-10-01 The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) associates with epigenetic aberrancy, including the abnormal acetylation of histones. Altering the epigenetic status by histone deacetylase inhibitors (HDACi) enhances the developmental potential of SCNT embryos. In the current study, we examined the effects of LBH589 (panobinostat), a novel broad-spectrum HDACi, on the nuclear reprogramming and development of pig SCNT embryos in vitro. In experiment 1, we compared the in vitro developmental competence of nuclear transfer embryos treated with different concentrations of LBH589. Embryos treated with 50 nM LBH589 for 24 hours showed a significant increase in the rate of blastocyst formation compared with the control or embryos treated with 5 or 500 nM LBH589 (32.4% vs. 11.8%, 12.1%, and 10.0%, respectively, P < 0.05). In experiment 2, we examined the in vitro developmental competence of nuclear transfer embryos treated with 50 nM LBH589 for various intervals after activation and 6dimethylaminopurine. Embryos treated for 24 hours had higher rates of blastocyst formation than the other groups. In experiment 3, when the acetylation of H4K12 was examined in SCNT embryos treated for 6 hours with 50 nM LBH589 by immunohistochemistry, the staining intensities of these proteins in LBH589-treated SCNT embryos were significantly higher than in the control. In experiment 4, LBH589-treated nuclear transfer and control embryos were transferred into surrogate mothers, resulting in three (100%) and two (66.7%) pregnancies, respectively. In conclusion, LBH589 enhances the nuclear reprogramming and developmental potential of SCNT embryos by altering the epigenetic status and expression, and increasing blastocyst quality. 132. Significance of ultrasonography in selecting methods for the treatment of acute cholecystitis Directory of Open Access Journals (Sweden) Grzegorz Ćwik 2013-09-01 Full Text Available Surgical removal of the gallbladder is indicated in nearly all cases of complicated acute cholecystitis. In the 1990s, laparoscopic cholecystectomy became the method of choice in the treatment of cholecystolithiasis. Due to a large inflammatory reaction in the course of acute inflammation, a laparoscopic procedure is conducted in technically difficult conditions and entails the risk of complications. The aim of this paper was: 1 to analyze ultrasound images in acute cholecystitis; 2 to specify the most common causes of conversion from the laparoscopic method to open laparotomy; 3 to determine the degree to which the necessity for such a conversion may be predicted with the help of ultrasound examinations. Material and methods: In 1993–2011, in the Second Department and Clinic of General, Gastroenterological and Oncological Surgery of the Medical University in Lublin, 5,596 cholecystectomies were performed including 4,105 laparoscopic procedures that constituted 73.4% of all cholecystectomies. Five hundred and forty-two patients (13.2% were qualified for laparoscopic procedure despite manifesting typical symptoms of acute cholecystitis in ultrasound examination, which comprise: thickening of the gallbladder wall of > 3 mm, inflammatory infiltration in the Calot’s triangle region, gallbladder filled with stagnated or purulent contents and mural or intramural effusion. Results: In the group of operated patients, the conversion was necessary in 130 patients, i.e. in 24% of cases in comparison with 3.8% of patients with uncomplicated cholecystolithiasis (without the signs of inflammation. The conversion most frequently occurred when the assessment of the anatomical structures of the Calot’s triangle was rendered more difficult due to local inflammatory process, mural effusion and thickening of the gallbladder wall of >5 mm. The remaining changes occurred more rarely. Conclusions: Based on imaging scans, the most common causes of conversion 133. Role and significance of polyunsaturated fatty acids in nutrition in prevention and treatment of atherosclerosis Directory of Open Access Journals (Sweden) Ristić Vanja I. 2003-01-01 Full Text Available Introduction Hyperlipoproteinemia is a key factor in development of atherosclerosis, whereas regression of atherosclerosis mostly depends on decreasing the plasma level of total and LDL-cholesterol. Many studies have reported the hypocholesterolemic effect of linolenic acid. Types of polyunsaturated fatty acids (PUFA Linoleic and αlinolenic acids are essential fatty acids. The main sources of linoleic acid are vegetable seeds and of α-linolenic acid - green parts of plants. α-linolenic acid is converted to eicosapentaenoic and docosahexaenoic acid. Linoleic acid is converted into arachidonic acid competing with eicosapentaenoic acid in the starting point for synthesis of eicosanoids, which are strong regulators of cell functions and as such, very important in physiology and pathophysiology of cardiovascular system. Eicosanoids derived from eicosapentaenoic acid have different biological properties in regard to those derived from arachidonic acid, i.e. their global effects result in decreased vasoconstriction platelet aggregation and leukocyte toxicity. Role and significant of PUFA The n-6 to n-3 ratio of polyunsaturated fatty acids in the food is very important, and an optimal ratio 4 to 1 in diet is a major issue. Traditional western diets present absolute or relative deficiency of n-3 polyunsaturated fatty acids, and a ratio 15-20 to 1. In our diet fish and fish oil are sources of eicosapentaenoic and docosahexaenoic acid. Refined and processed vegetable oils change the nature of polyunsaturated fatty acids and obtained derivates have atherogenic properties. 134. The significance of recurrent lung opacities in neonates on surfactant treatment for respiratory distress syndrome Energy Technology Data Exchange (ETDEWEB) Odita, J.C. [Dept. of Radiology, Louisiana State Univ. Health Sciences Center, Shreveport (United States) 2001-02-01 Purpose. To determine the significance of recurrent opacities in chest radiographs of neonates on surfactant therapy for respiratory distress syndrome (RDS) after an initial period of improvement. Materials and methods. Serial pre- and post-surfactant chest radiographs on 94 preterm infants with RDS were analyzed and the pattern of chest radiographic response was classified as (a) clear, (b) recurrent opacities, and (c) no response. Their clinical characteristics were also recorded. Results. In 34 infants the RDS changes cleared within 3 days. 31 infants developed lung opacities within 10 days after an initial period of improvement. Twenty-nine infants failed to respond to the surfactant. The corresponding mean birth weights for the three groups were 1.74, 1.19, and 0.76 kg and the mean gestation ages 32.6, 27.7, and 25.4 weeks. The incidence of bronchopulmonary dysplasia (BPD) was highest among the slumping infants (72. % vs 50 % in no responders, P < 0.001) Conclusions. The pattern of chest radiographic response is primarily affected by gestation age and birth weight. Recurrent lung opacity after an initial positive response to surfactant therapy may be caused by such factors as edema from barotrauma and patent ductus arteriosus. Infants with intraventricular hemorrhage may demonstrate neurogenic edema. Other contributory factors include pneumonia and abnormal consumption of surfactant. Recurrent lung opacities after surfactant may be a predictor of chronic lung disease in the preterm infant. (orig.) 135. Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane. Science.gov (United States) Garcia-Celma, Juan J; Dueck, Benjamin; Stein, Martin; Schlueter, Michela; Meyer-Lipp, Kerstin; Leblanc, Gerard; Fendler, Klaus 2008-08-01 Rapid solution exchange on a solid-supported membrane (SSM) is investigated using fluidic structures and a solid-supported membrane of 1 mm diameter in wall jet geometry. The flow is analyzed with a new technique based on specific ion interactions with the surface combined with an electrical measurement. The critical parameters affecting the time course of the solution exchange and the transfer function describing the time resolution of the SSM system are determined. The experimental data indicate that solution transport represents an intermediate situation between the plug flow and the Hagen-Poiseuille laminar flow regime. However, to a good approximation the rise of the surface concentration can be described by Hagen-Poiseuille flow with ideal mixing at the surface of the SSM. Using an improved cuvette design, solution exchange as fast as 2 ms was achieved at the surface of a solid-supported membrane. As an application of the technique, the rate constant of a fast electrogenic reaction in the melibiose permease MelB, a bacterial ( Escherichia coli) sugar transporter, is determined. For comparison, the kinetics of a conformational transition of the same transporter was measured using stopped-flow tryptophan fluorescence spectroscopy. The relaxation time constant obtained for the charge displacement agrees with that determined in the stopped-flow experiments. This demonstrates that upon sugar binding MelB undergoes an electrogenic conformational transition with a rate constant of k approximately 250 s (-1). 136. Qualidade de melões ‘Louis‘ armazenados em quatro temperaturas Directory of Open Access Journals (Sweden) Cristiane Maria Ascari Morgado 2015-11-01 Full Text Available Este trabalho teve como objetivo estabelecer a melhor temperatura para a conservação de melões ‘Louis‘. Os frutos foram armazenados sob condiçà £o de ambiente (22±2°C e 80±5% UR, sob refrigeração (3±1°C e 80±5% UR; 6±1°C e 80±5% UR; e 9±1°C e 80±5% UR e avaliados quanto à coloração e firmeza da polpa, teores de sólidos solúveis, acidez titulável, atividade das enzimas peroxidase, polifenoloxidase, poligalacturonase e pectinametilesterase e perda de massa fresca. Os melões ‘Louis‘ armazenados a 22°C apresentaram maior redução nos teores de sólidos solúveis e acidez titulável, além de apresentarem maior atividade da enzima poligalacturonase, com conservação por até 18 dias. Os frutos armazenados a 6°C mantiveram a qualidade comercial durante 25 dias de armazenamento, apresentando os maiores teores de sólidos solúveis e menor atividade das enzimas peroxidase e poligalacturonase.

137. Mel de abelhas sem ferrão: proposta de regulamentação Directory of Open Access Journals (Sweden) Ricardo Costa Rodrigues de Camargo Full Text Available Resumo Os méis das abelhas sem ferrão são produtos únicos da biodiversidade brasileira, presentes e valorizados pela cultura popular desde os povos originais das Américas. Por ser mais ácido e apresentar maior teor de umidade, seu sabor é peculiar e sua viscosidade é menor quando comparada ao mel proveniente da espécie exótica Apis mellifera. Devido a essas diferenças, esse produto necessita do estabelecimento de parâmetros fÃsico-quÃmicos especÃficos que sejam referência tanto para controle de qualidade como para sua comercialização. Com base em pesquisas realizadas pela Embrapa, pelo Instituto de Tecnologia de Alimentos de Campinas e pelo levantamento bibliográfico de diversos estudos cientÃficos realizados por outros institutos de ensino e pesquisa, esta comunicação tem como objetivo esclarecer as peculiaridades deste produto e apresentar uma proposta de Regulamento Técnico de Identidade e Padrão para o Mel das Abelhas Sem Ferrão, visando sua comercialização no Estado de São Paulo, contribuindo assim para a disponibilidade de um produto seguro e de qualidade para o consumidor final. 138. Mel-frequencies Stochastic Model for Gender Classification based on Pitch and Formant Directory of Open Access Journals (Sweden) Syifaun Nafisah 2016-02-01 Full Text Available Speech recognition applications are becoming more and more useful nowadays. Before this technology is applied, the first step is test the system to measure the reliability of system. The reliability of system can be measured using accuracy to recognize the speaker such as speaker identity or gender. This paper introduces the stochastic model based on mel-frequencies to identify the gender of speaker in a noisy environment. The Euclidean minimum distance and back propagation neural networks were used to create a model to recognize the gender from his/her speech signal based on formant and pitch of Mel-frequencies. The system uses threshold technique as identification tool. By using this threshold value, the proposed method can identifies the gender of speaker up to 94.11% and the average of processing duration is 15.47 msec. The implementation result shows a good performance of the proposed technique in gender classification based on speech signal in a noisy environment. 139. Qualidade dos produtos apÃcolas da Guiné Bissau: mel e própolis OpenAIRE Lopes, Mélissa Andréa 2014-01-01 A colmeia é uma fonte de produtos nutritivos e com elevadas potencialidades farmacológicas, como o mel, o pólen, a cera, a geleia real, a apitoxina ou o pão de abelha. Neste trabalho destacamos o mel e a própolis da Guiné- Bissau avaliando a sua qualidade. Na Guiné- Bissau, um paÃs subdesenvolvido situado na costa Ocidental de Ãfrica, os produtos provenientes da colmeia podem contribuir para o enriquecimento das condições nutricionais da população um problema que se vem mantendo ao longo d... 140. Análisis melódico de la ironÃa en el discurso televisivo OpenAIRE 2014-01-01 El principal objetivo de nuestro trabajo consistirá en describir los diferentes patrones melódicos que contribuyen a definir la ironÃa como fenómeno pragmático dentro del complejo funcionamiento de la entonación en el ámbito de la conversación coloquial. Tal objetivo se desarrollará a través de un estudio fonético-fonológico basado en el procedimiento experimental del modelo de Análisis Melódico del Habla (AMH) expuesto detalladamente en Cantero (2002). En definitiva, estudiaremos las diferen... « 5 6 7 8 9 » « 6 7 8 9 10 » 141. Avaliação dos NÃveis de Contaminação dos Antibióticos no mel Português OpenAIRE Correia, Daniela M.; Vilas-Boas, Miguel; Dias, L. G. 2006-01-01 A presença residual dos antibióticos no mel é um problema actual com implicações negativas, principalmente comerciais, desde que, de acordo com a legislação europeia, a ocorrência de antibióticos em. amostras do mel é proibida 142. Functional analysis of a recombinant PIII-SVMP, GST-acocostatin; an apoptotic inducer of HUVEC and HeLa, but not SK-Mel-28 cells. Science.gov (United States) Teklemariam, Takele; Seoane, Agustin I; Ramos, Carla J; Sanchez, Elda E; Lucena, Sara E; Perez, John C; Mandal, Stephanie A; Soto, Julio G 2011-04-01 Disintegrins and disintegrin-like peptides interact with integrins and interfere with cell-cell and cell-matrix interactions. A disintegrin-like snake venom gene, Acocostatin was cloned from the venom gland mRNA of Agkistrodon contortrix contortrix. Acocostatin belongs to the PIII-SVMP subfamily of disintegrin-like peptides. The recombinant acocostatin peptide was produced and purified as GST-fusion. The GST-acocostatin peptide, at 44 μg/mL, inhibited platelet aggregation by 30% in PRP and 18% in whole blood. In addition GST-acocostatin, at 220 μg/mL, inhibited SK-Mel-28 cell migration by 48%, but did not inhibit T24 cell migration. The GST-acocostatin peptide ability to induce apoptosis on HUVEC, HeLa, and SK-Mel-28 cells was determined using Annexin V-FITC and chromatin fragmentation assays after 24 h of treatment. At 5 μM GST-acocostatin peptide, 19.68%+/- 3.09 of treated HUVEC, and 35.86% +/- 2.05 of treated HeLa cells were in early apoptosis. The GST-acocostatin peptide also caused chromatin fragmentation of HUVEC and HeLa cells as determined by fluorescent microscopy and Hoechst staining. The GST-acocostatin peptide failed to induce apoptosis of SK-Mel-28 cells. We characterized the HUVEC, HeLa, and T24 integrin expression by flow cytometry, as the first step in determining GST-acocostatin binding specificity. Our results indicate that HUVEC express αv, αvβ3, αvβ5, α6, β1, and β3 integrin receptors. HeLa cells express α1, α2, α6, αv, αvβ5, and β1 integrin receptors. T24 cells express α1, α3, α6, Î ±v, αvβ3, αvβ5, β1, β3, and β6 integrin receptors. 143. Prognostic Significance of Initial Serum Albumin and 24 Hour Daily Protein Excretion before Treatment in Multiple Myeloma. Directory of Open Access Journals (Sweden) Jia-Hong Chen Full Text Available Renal failure is a common morbidity in multiple myeloma (MM. Although proteinuria has been increasingly reported in malignancies, it is not routinely used to refine risk estimates of survival outcomes in patients with MM. Here we aimed to investigate initial serum albumin and 24-hour daily protein excretion (24-h DPE before treatment as prognostic factors in patients with MM. We conducted a retrospective analysis of 102 patients with myeloma who were ineligible for haematopoietic stem cell transplantation between October 2000 and December 2012. Initial proteinuria was assessed before treatment by quantitative analysis of 24-hour urine samples. The demographic and laboratory characteristics, survival outcome, and significance of pre-treatment 24-h DPE and albumin in the new staging system of MM were analyzed. Pre-treatment proteinuria (>300 mg/day was present in 66 patients (64.7%. The optimal cut-off value of 24-h DPE before treatment was 500 mg/day. Analysis of the time-dependent area under the curve showed that the serum albumin and 24-h DPE before treatment were better than 24-h creatinine clearance rate and β2-microglobulin. A subgroup analysis showed that an initial excess proteinuria (24-h DPE ≥ 500 mg was associated with poor survival status (17.51 vs. 34.24 months, p = 0.002. Furthermore, initial serum albumin was an independent risk factor on multivariate analysis (300 mg/day) was present in 66 patients (64.7%). The optimal cut-off value of 24-h DPE before treatment was 500 mg/day. Analysis of the time-dependent area under the curve showed that the serum albumin and 24-h DPE before treatment were better than 24-h creatinine clearance rate and β2-microglobulin. A subgroup analysis showed that an initial excess proteinuria (24-h DPE ≥ 500 mg) was associated with poor survival status (17.51 vs. 34.24 months, p = 0.002). Furthermore, initial serum albumin was an independent risk factor on multivariate analysis (albumin and 24-h DPE before treatment showed significant prognostic factors in patients with MM, and the new A-DPE staging system may be utilized instead of the International Staging System. Its efficacy should be evaluated by further large prospective studies. 146. Significant social events and increasing use of life-sustaining treatment: trend analysis using extracorporeal membrane oxygenation as an example. Science.gov (United States) Chen, Yen-Yuan; Chen, Likwang; Huang, Tien-Shang; Ko, Wen-Je; Chu, Tzong-Shinn; Ni, Yen-Hsuan; Chang, Shan-Chwen 2014-03-04 Most studies have examined the outcomes of patients supported by extracorporeal membrane oxygenation as a life-sustaining treatment. It is unclear whether significant social events are associated with the use of life-sustaining treatment. This study aimed to compare the trend of extracorporeal membrane oxygenation use in Taiwan with that in the world, and to examine the influence of significant social events on the trend of extracorporeal membrane oxygenation use in Taiwan. Taiwan's extracorporeal membrane oxygenation uses from 2000 to 2009 were collected from National Health Insurance Research Dataset. The number of the worldwide extracorporeal membrane oxygenation cases was mainly estimated using Extracorporeal Life Support Registry Report International Summary July 2012. The trend of Taiwan's crude annual incidence rate of extracorporeal membrane oxygenation use was compared with that of the rest of the world. Each trend of extracorporeal membrane oxygenation use was examined using joinpoint regression. The measurement was the crude annual incidence rate of extracorporeal membrane oxygenation use. Each of the Taiwan's crude annual incidence rates was much higher than the worldwide one in the same year. Both the trends of Taiwan's and worldwide crude annual incidence rates have significantly increased since 2000. Joinpoint regression selected the model of the Taiwan's trend with one joinpoint in 2006 as the best-fitted model, implying that the significant social events in 2006 were significantly associated with the trend change of extracorporeal membrane oxygenation use following 2006. In addition, significantly social events highlighted by the media are more likely to be associated with the increase of extracorporeal membrane oxygenation use than being fully covered by National Health Insurance. Significant social events, such as a well-known person's successful extracorporeal membrane oxygenation use highlighted by the mass media, are associated with the use of 147. IAEA-MEL case studies. Pt.1. Source terms and transport processes Energy Technology Data Exchange (ETDEWEB) Baxter, M.S. [International Atomic Energy Agency, Monaco (Monaco). Marine Environmental Laboratory 1997-12-31 This IAEA review summarises the nuclide inventory and process-related aspects of investigations at sites which include disposals and dispersions of radioactive waste in the Arctic Seas, N.E. Atlantic Ocean, Far Eastern Seas and the Irish Sea, accidental inputs to the Norwegian Sea and Sea of Okhotsk, releases from weapons test environments in the Pacific Ocean and fallout from the Chernobyl accident, particularly in the Baltic and Black Seas. In many of these cases, monitoring, behavioural and modelling studies have been carried out. The Monaco laboratory is using natural and man-made radionuclides to trace particle flux and sedimentation, particularly in S.E. Asia and the Mediterranean Sea. A perspective on the results of these studies has been maintained in a new and growing marine radioactivity database which will soon be available to Member States. Radiological conclusions can be compared to the recently published results of a coordinated research programme organised by IAEA-MEL on sources of radioactivity in the marine environment and their relative contributions to overall dose assessment from marine radioactivity (the MARDOS project). Finally, some future plans within the IAEA-MEL programme are outlined. For example, the Government of Japan is funding a strategically planned follow-up to the Geosecs programme to update information on open ocean radionuclide distributions. Additional IAEA funds have also been allocated for study of marine inputs of technologically enhanced natural radionuclides from the oil and phosphogypsum industries. The laboratory is also developing and applying in-situ monitoring techniques for continuous and ROV-mounted surveys of radionuclide inventories in water and sediment. The presentation will end with a call for participation in an IAEA Symposium on Marine Pollution, containing various sessions related to tracer and radiological aspects of marine radioactivity, to be held in Monaco in 1998 as part of the United Nations programme 148. AVALIAÇÃO DA QUALIDADE FÃSICO-QUÃMICA DO MEL PRODUZIDO NA CIDADE DE PELOTAS/RS Directory of Open Access Journals (Sweden) Willian RICHTER 2011-12-01 Full Text Available O trabalho teve por objetivo avaliar a qualidade fÃsico-quÃmica de dezenove amostras de méis produzidas na cidade de Pelotas/RS. Foram realizadas análises de umidade, cinzas, acidez total, açúcares redutores e não redutores, hidroximetilfurfural, condutividade elétrica, reações de Fiehe e Lund. Das dezenove amostras avaliadas, dezoito pela determinação da condutividade elétrica, foram classificadas como sendo mel de melato, mel de castanha ou mistura das mesmas. Em relaçà £o à s demais análises, uma amostra foi considerada adulterada por apresentar inconformidade em relação ao teor umidade, cinzas, açúcares redutores, reação de Lund e reação de Fiehe confirmada pela quantificação de hidroximetilfurfural. Assim, devido à possibilidade de fraude, deve haver maior fiscalização dos órgãos competentes, quanto à produção e comercialização de mel. O consumidor deverá atentar-se ao adquirir mel em feiras livres e estabelecimentos onde os produtos são comercializados sem certificação. 149. A Theoretically Consistent Method for Minimum Mean-Square Error Estimation of Mel-Frequency Cepstral Features DEFF Research Database (Denmark) Jensen, Jesper; Tan, Zheng-Hua 2014-01-01 the logarithmic which is usually used for MFCC computation. The proposed method shows estimation performance which is identical to or better than state-of-the-art methods. It further shows comparable ASR performance, where the advantage of being able to use mel-frequency speech features based on a power non......-linearity rather than a logarithmic is demonstrated.... 150. Producción y exportación de melón (Cucumis melo en Costa Rica Directory of Open Access Journals (Sweden) José Eladio Monge-Pérez 2014-02-01 Full Text Available Se estudió la producción y exportación de melón en Costa Rica. Se recopiló y analizó información obtenida a través de diferentes fuentes (FAO, BCCR, PROCOMER, MAG, CANAPEMS. Se estableció comunicación personal con gerentes de empresas exportadoras de melón y se revisaron diversas publicaciones sobre el tema. En este artÃculo se presentan datos sobre el área de siembra, el número de empresas exportadoras, la distribución geográfica del cultivo, el valor de las exportaciones, las exportaciones por paÃs de destino y por tipo de melón a lo largo de varios años. Finalmente, se analizan los desafÃos a nivel agronómico, económico, ambiental y logÃstico para el futuro de la producción de melón en Costa Rica. 151. Armazenamento sob condições ambiente e aceitabilidade do melão 'F1 Jangada' produzido em sistema hidropônico Storage under atmosphere conditions and acceptability of the melon 'F1 Jangada' croped in hydroponic system Directory of Open Access Journals (Sweden) Maria M. Rinaldi 2006-12-01 Full Text Available O objetivo deste trabalho foi determinar o perÃodo de armazenamento pós-colheita e a aceitabilidade pelo consumidor de melão hÃbrido 'F1 Jangada' (Cucumis melo L., produzido em sistema hidropônico, mantido em condições ambiente (22 ± 2 ºC e umidade relativa de 40 ± 5%. O experimento compreendeu o perÃodo de 21-6-2005 a 2-8-2005. Foi utilizado o esquema fatorial 5 x 2, em delineamento experimental inteiramente casualizado, com cinco perÃodos de armazenamento (0; 7; 21; 28 e 42 dias e dois tipos de substrato (areia e fibra de coco, com três repetições, em que cada repetição consistiu em cinco frutos de meloeiro. Foram avaliados o pH, a acidez titulável, os sólidos solúveis, a perda de massa fresca, a análise sensorial e a decisão de compra dos melões. Foram verificados efeitos do tipo de substrato e tempo de armazenamento sobre os valores de pH dos melões. A acidez titulável dos melões diminuiu significativamente nos primeiros sete dias de armazenamento, em ambos os substratos. Não foram verificados efeitos do tipo de substrato e tempo de armazenamento nos sólidos solúveis dos melões durante o armazenamento. Não houve diferença de perda de massa fresca dos frutos produzidos nos dois substratos, sendo de 7,1 ± 0,2%, durante os 42 dias de armazenamento. O tipo de substrato não interferiu na aparência geral, cor, textura e sabor dos melões. Aos 42 dias de armazenamento, os melões produzidos nos dois tipos de substrato apresentaram-se aceitáveis pelo consumidor. No entanto, os produzidos no substrato com areia apresentaram melhor aceitabilidade e decisão de compra ao longo do armazenamento.The objective of this work was to evaluate the storage period postharvest and acceptability by consumer of hybrid melon 'F1 Jangada' (Cucumis melo L., produced in hydroponic system, stored in atmosphere conditions (22 ± 2 ºC and 40 ± 5% relative humidity. The research was carried from June 21st to August 2nd, 2005. It was 152. The significance of swimming and corrective exercises in water in the treatment of postural deficits and scoliosis OpenAIRE Åubkowska, Wioletta; PaczyÅ„ska-JÄ™drycka, MaÅ‚gorzata; Eider, Jerzy 2014-01-01 More and more often specialized literature mentions the contemporarily relevant notion of the application of swimming as one of the therapeutic methods in modern medicine. The thesis reviews specialized literature and analyzes documents in order to demonstrate the significance of aquatic therapy and corrective swimming exercises as a corrective and therapeutic function in the treatment of postural deficits and scoliosis. As this article has a character of a review, its purpose is to ... 153. Significant treatment effect of add-on ketamine anesthesia in electroconvulsive therapy in depressive patients: A meta-analysis. Science.gov (United States) Li, Dian-Jeng; Wang, Fu-Chiang; Chu, Che-Sheng; Chen, Tien-Yu; Tang, Chia-Hung; Yang, Wei-Cheng; Chow, Philip Chik-Keung; Wu, Ching-Kuan; Tseng, Ping-Tao; Lin, Pao-Yen 2017-01-01 Add-on ketamine anesthesia in electroconvulsive therapy (ECT) has been studied in depressive patients in several clinical trials with inconclusive findings. Two most recent metaanalyses reported insignificant findings with regards to the treatment effect of add-on ketamine anesthesia in ECT in depressive patients. The aim of this study is to update the current evidence and investigate the role of add-on ketamine anesthesia in ECT in depressive patients via a systematic review and meta-analysis. We performed a thorough literature search of the PubMed and ScienceDirect databases, and extracted all relevant clinical variables to compare the antidepressive outcomes between add-on ketamine anesthesia and other anesthetics in ECT. Total 16 articles with 346 patients receiving add-on ketamine anesthesia in ECT and 329 controls were recruited. We found that the antidepressive treatment effect of add-on ketamine anesthesia in ECT in depressive patients was significantly higher than that of other anesthetics (p9.0 log10 CFU.mL-1 were maintained in the presence of honey. The titratable acidity produced by these cultures was of 1.44%. On the 46th day of storage, the number of L. acidophilus Sacco® viable cells in the presence of honey was significantly higher (p < 0.05 compared to the control. Considering the overall storage period, honey exerted significant positive effect (p < 0,05 only on Bifidobacterium cultures. The lowest growth and acidity on the 46th day was observed with Bf. Lactis Bb12, being 7,63 log10 CFU.g-1 and 0,61% of acidity in the presence of honey and 6.11 log CFU.mL-1 and 0,30% of acidity in the control. Differently, Bf. lactis Sacco® cultures reached counts of 9,11 log10 CFU 173. Flora asociada a la actividad melÃfera en apiarios al sur del departamento del Huila, macizo colombiano Directory of Open Access Journals (Sweden) Rudas Lleras AgustÃn 2006-12-01 Full Text Available Este trabajo hace parte del proyecto “Diversificación de zonas de ladera con productos de alto valor: mieles especielesâ€, desarrollado por el Instituto Humboldt, el Centro Internacional de Agricultura Tropical, la Universidad Nacional de Colombia y la Corporación Autónoma Regional del Alto Magdalena, que pretende fortalecer cadenas productivas alternativas para el sustento de los campesinos de zonas cafeteras del Macizo Colombiano. Se caracterizó la flora aledaña a cuatro apiarios localizados en los municipios La Argentina, Palestina y Pitalito del sur del departamento del Huila y se establecieron los elementos florÃsticos más importantes para la actividad melÃfera. El reconocimiento florÃstico se realizó en el radio de un kilómetro, alrededor de los apiarios y mediante colecciones botánicas en todas las coberturas vegetales y ambientes hallados. En total se visitaron 44 sitios entre septiembre y noviembre de 2005. Se colectaron 1.091 especÃmenes correspondientes a 657 especies y 112 familias. La familias con mayor número de especies fueron Asteraceae, seguida por Fabaceae s l y Poaceae; se encontró una riqueza alta a nivel de familia y especie, lo que se relacionó con la existencia de numerosos tipos y manejos de cobertura vegetal, usos del suelo y una frecuente intervención humana. Se encontraron especies de rara distribución en Colombia y otras poco colectadas, lo que soporta la idea de la baja exploración frente a la alta riqueza que actualmente presenta la flora del Macizo Colombiano. Se determinaron 135 de las especies como melÃferas, la mayorÃa pertenecientes a las familias Asteraceae, Fabaceae s l y Lamiaceae. Se hallaron 87 especies melÃferas en La Argentina, 57 en Palestina y 43 en Pitalito. Seis de las especies melÃferas encontradas se establecieron como las más importantes por presentar los mayores Ãndices de importancia ecológica (Saurauia aromatica, Cuphea 174. Qualidade de melão rendilhado (Cucumis melo L.) em função do sistema de cultivo OpenAIRE Vargas, Pablo Forlan [UNESP; Castoldi,Renata; Charlo, Hamilton César de Oliveira [UNESP; Braz, Leila Trevizan [UNESP 2008-01-01 Objetivou-se com este trabalho avaliar a qualidade de frutos de cinco cultivares de melão rendilhado (Cucumis melo L.), cultivados em casa de vegetação, em função do sistema de produção. O experimento foi instalado em casa de vegetação na UNESP-FCAV, Jaboticabal-SP, no perÃodo de novembro de 2005 à fevereiro de 2006. O experimento foi delineado em esquema fatorial 5 X 2, em blocos casualizados com quatro repetições. Os tratamentos resultaram da combinação de cinco hÃbridos de melà £o rendilhado... 175. Actividade antifúngica de extractos fenólicos do mel de Trás-os-Montes OpenAIRE Barros, Susana; Calhelha, Ricardo C.; Estevinho, Leticia M. 2005-01-01

O mel é considerado um produto alimentar benéfico e promotor de saúde, através da prevenção de doenças. Estas propriedades devem-se em parte a presença de compostos fenólicos (ácidos fenólicos e flavonóides) na sua composição. 176. Caracterização fÃsico-quÃmica de cervejas elaboradas com mel Directory of Open Access Journals (Sweden) Luciana Trevisan Brunelli 2014-03-01 Full Text Available O objetivo do presente trabalho foi produzir e caracterizar fÃsico-quimicamente cervejas elaboradas com mel. Os ensaios de produção de cerveja foram feitos com nove tratamentos, combinação de três concentrações de extrato original (11, 13 e 15 ºBrix e três porcentagens de mel na formulação do mosto (0%, 20% e 40%. O experimento foi inteiramente casualizado com duas repetições, perfazendo dezoito parcelas experimentais. A mosturação foi realizada pelo processo de infusão, sendo o mel adicionado na etapa de fervura. Depois de clarificado, o mosto teve seu teor de extrato corrigido com água, sendo inoculado com levedura de baixa fermentação. A fermentação ocorreu a 10 ºC. A cerveja foi engarrafada manualmente e armazenada em freezer à temperatura de 0 ºC por 15 dias, para sua maturação. As cervejas foram analisadas quanto ao teor alcoólico, extrato real, extrato aparente, cor, amargor, turbidez, pH, acidez total e gás carbônico, densidade de espuma e total de espuma. Os resultados das análises fÃsico-quÃmicas das cervejas foram submetidos à análise de variância e as médias foram comparadas pelo teste de Tukey, ao nÃvel de 5% de probabilidade. Todas as cervejas foram classificadas como claras. A presença de mel na formulação favoreceu a carbonatação, aumentou a densidade de espuma e total de espuma, mas, em contrapartida, as cervejas ficaram menos amargas e com menor acidez. 177. Significance of depression in obstructive sleep apnea patients and the relationship between the comorbidity and continuous positive airway pressure treatment Institute of Scientific and Technical Information of China (English) FENG Jing; CHEN Bao-yuan; Ambrose An-Po Chiang 2010-01-01 Obstructive sleep apnea (OSA), which is the most common sleep-related breathing disorder and characterized by recurrent collapse of the upper airway,causes repeated arousals from sleep, decreased oxygen saturation of the blood, and excessive daytime sleepiness.Patients with OSA are at increased risk of cardiovascular and cerebrovascular disease, type 2 diabetes, cognitive impairment, and depression.1,2 Currently, continuous positive airway pressure (CPAP) had been identified as the standard treatment for patients with OSA. CPAP shows significant improvements in objective and subjective sleepiness and several quality3 of life assessments, cognitive function and depression.3 However,reports of nonadherence to CPAP therapy range from 29% to 83%, and one of the reasons of non-adherence is the comorbidity of depression.4 178. Significance of serum fucose, sialic acid, haptoglobine and phospholipids levels in the evolution and treatment of breast cancer. Science.gov (United States) Kiricuta, I; Bojan, O; Comes, R; Cristian, R 1979-01-01 Serum fucose, sialic acid, haptoglobine and phospholipids were determined in 167 women with breast cancer stages I--III, 30 with benign lesions of the breast, 42 women in various physiological states of the mammary gland (pregnancy, early childbed and lactation) and compared with 30 healthy women as control. Serial determinations of these parameters during the radio-surgical treatment were done in 28 patients with breast cancer stage III. Fucose and phospholipids levels were significantly increased respectively decreased in the group of patients with breast cancers but unmodified in the others. Sialic acid and haptoglobine -- increased in patients with cancer -- were also elevated in patients with early childbed and benign affections of the breast. The surveillance of these four parameters during the radio-surgical treatment of breast cancer evidenced a good correlation between their modified levels and clinical state of the patients. The increase in fucose, sialic acid and haptoglobine respectively the decrease in phospholipids levels was associated with the clinical evidence of recurrences and metastases. 179. Text Dependent Speaker Identification Using a Bayesian network and Mel Frequency Cepstrum Coefficient Directory of Open Access Journals (Sweden) Mohd. Manjur Alam 2014-12-01 Full Text Available Speaker identification is a biometric technique. The objective of automatic speaker recognition is to extract, characterize and recognize the information about speaker identity. Speaker Recognition technology has recently been used in large number of commercial areas successfully such as in voice based biometrics; voice controlled appliances, security control for confidential information, remote access to computers and many more interesting areas. A speaker identification system has two phases which are the training phase and the testing phase. Feature extraction is the first step for each phase in speaker recognition. Many algorithms are suggested by the researchers for feature extraction. In this work, the Mel Frequency Cepstrum Coefficient (MFCC feature has been used for designing a text dependent speaker identification system. While, in the identification phase, the existing reference templates are compared with the unknown voice input. In this thesis, a Bayesian network is used as the training/recognition algorithm which makes the final decision about the specification of the speaker by comparing unknown features to all models in the database and selecting the best matching model. i, e. the highest scored model. The speaker who obtains the highest score is selected as the target speaker. 180. Stability of the wMel Wolbachia Infection following invasion into Aedes aegypti populations. Directory of Open Access Journals (Sweden) Ary A Hoffmann 2014-09-01 Full Text Available The wMel infection of Drosophila melanogaster was successfully transferred into Aedes aegypti mosquitoes where it has the potential to suppress dengue and other arboviruses. The infection was subsequently spread into two natural populations at Yorkeys Knob and Gordonvale near Cairns, Queensland in 2011. Here we report on the stability of the infection following introduction and we characterize factors influencing the ongoing dynamics of the infection in these two populations. While the Wolbachia infection always remained high and near fixation in both locations, there was a persistent low frequency of uninfected mosquitoes. These uninfected mosquitoes showed weak spatial structure at both release sites although there was some clustering around two areas in Gordonvale. Infected females from both locations showed perfect maternal transmission consistent with patterns previously established pre-release in laboratory tests. After >2 years under field conditions, the infection continued to show complete cytoplasmic incompatibility across multiple gonotrophic cycles but persistent deleterious fitness effects, suggesting that host effects were stable over time. These results point to the stability of Wolbachia infections and their impact on hosts following local invasion, and also highlight the continued persistence of uninfected individuals at a low frequency most likely due to immigration. « 7 8 9 10 11 » « 8 9 10 11 12 » 181. Removal of prioritary pesticides contamining r'mel ground water by using organic waste residues. Science.gov (United States) El Bakouri, H; Morillo, J; Usero, J; Ouassini, A 2007-01-01 This study evaluated pesticide contamination of R'mel ground water located in northwest Morocco. The study area is densely populated and thriving, with intensive agriculture. Various techniques, including stir bar sorptive extraction (SBSE) and gas chromatography with mass spectroscopy detection (GC-MS), were used for the quantitative determination of 13 pesticides including alachlor, aldrin, atrazine, chlorpyrifos, chlorfenvinphos, dieldrin, alpha-endosulfan, endrin, hexachlorobenzene, beta-HCH, gamma-HCH (lindane), simazine and trifluralin. The survey results showed that contamination by pesticide residues is widespread in the area. With the exception of atrazine, the average concentrations were all below the regulatory limits established by the European Union. The potential of ten natural organic substances to eliminate pesticides included in the European Water Framework Directive was evaluated. The absorbents with the highest removal efficiency were date and olives stones and, to a Lesser degree, Raphanus raphanistrum and Cistus ladaniferus. The adsorption tests gave very satisfying results and pointed to the possible application of these supports as ecoLogical remediation techniques to prevent pesticide pollution of aquatic ecosystems. 182. Food bike Rosa Mel: viabilidade do empreendimento por meio de Plano de Negócio Directory of Open Access Journals (Sweden) Danielle de Oliveira Barutti 2016-02-01 Full Text Available Este artigo objetiva propor a criação de um comércio itinerante de doces caseiros comercializando pão de mel, nos sabores brigadeiro, doce de leite e coco, trufas tradicionais, alfajor, café e água na modalidade Food Bike, na cidade de São Paulo. A modalidade Food Bike é o tipo de comércio itinerante em que se vendem comidas e bebidas, tendo como suporte uma bicicleta e não havendo necessidade de muito espaço para sua instalação. Utiliza-se como metodologia de análise, plano de negócio e pesquisa qualitativa exploratória para verificar o perfil do comércio itinerante e analisar o consumidor desse serviço. Foi concluÃdo que este tipo de empreendimento é inovador, atraente, de baixo custo inicial, rentável e com taxa de retorno em torno de onze meses. 183. Observation of effect of comprehensive community nursing On patients with hypertension and diabetes mel itus%高血压糖尿病患者的护ç†å¹²é¢„措施探讨 Institute of Scientific and Technical Information of China (English) 刘仕ç¼; åˆ˜æƒ 2014-01-01 目的：制订高血压糖尿病患者护ç†å¹²é¢„措施，探讨综åˆæŠ¤ç†å¹²é¢„对高血压糖尿病患者康å¤çš„å½±å“.æ–¹æ³•ï¼šé€ ‰æ‹©60例血压糖尿病患者，建立个人å¥åº·æ¡£æ¡ˆï¼Œç»™äºˆå¥åº·æ•™è‚²ã€é¥®é£ŸæŽ§åˆ¶ã€è¿åŠ¨ç–—法ç‰å¹²é¢„措施.比较干预å‰åŽæ‚£è€… éµåŒ»è¡Œä¸ºåŠè¡€ç³–æŒ‡æ ‡æŽ§åˆ¶æƒ…å†µ.结果：护ç†å¹²é¢„åŽï¼Œæ‚£è€…对高血压ã€ç³–å°¿ç—…ç›¸å…³çŸ ¥è¯†çš„掌æ¡ç¨‹åº¦æ˜Žæ˜¾ä¸Šå‡(P＜0．01)；干预åŽæ‚£è€…ç©ºè…¹è¡€ç³–å’Œç³–åŒ–è¡€çº¢è›‹è¾ƒå¹²é¢„å ‰æ˜Žæ˜¾ä¸‹é™ï¼Œ(P＜0．05).结论：对高血压糖尿病患者实施综åˆæŠ¤ç†å¹²é¢„治疗，效果良好.%Objective:To establish nursing intervention for patients with hypertension and diabetes mel itus and discuss influence of community nursing on rehabilitation of patients with hypertension and diabetes mel itus.Methods:Patients with confirmed diagnosis of hypertension and diabetes mel itus (60 cases)were selected,individual health records were established and heal h education was given to them,give health education,diet control,exercise therapy and other interventions.Compare compliance of patients before and after intervention and control of blood sugar level.Result:Nursing intervention,patients with hypertension,diabetes-related knowledge levels inG creased significantly (P