Nisin Resistance in Listeria monocytogenes ATCC 700302 Is a ... [PDF]

In addition to a lower ratio of C15 to C17 fatty acids than in the wild-type strain (A. S. Mazzotta and T. J. Montville,

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Appl Environ Microbiol. 1998 Jan; 64(1): 231–237.

PMCID: PMC124699

Nisin Resistance in Listeria monocytogenes ATCC 700302 Is a Complex Phenotype† Allison D. Crandall‡ and Thomas J. Montville* Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901-8520 * Corresponding author. Mailing address: Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers, The State University of New Jersey, 65 Dudley Rd., New Brunswick, NJ 08901-8520. Phone: (732) 932-9611, ext. 201. Fax: (732) 932-6776. E-mail: [email protected]. ‡ Present address: Department of Food Science, Cornell University, Ithaca, NY 14853. Received 1997 Jul 24; Accepted 1997 Oct 24. Copyright © 1998, American Society for Microbiology This article has been cited by other articles in PMC.

ABSTRACT

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MATERIALS AND METHODS

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Bacterial cultures, growth media, and nisin stock preparations.

Identification and quantification of membrane phospholipids.

Lysozyme sensitivity.

MIC determinations.

Transmission EM.

Determination of the PMF, pH gradient, and Δ.

Divalent-cation requirement of Nisr L. monocytogenes.

Determination of bacteriocin cross-resistance.

RESULTS

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Phospholipid composition.

Lysozyme sensitivity.

MIC determinations.

Transmission EM.

PMF measurements.

Divalent-cation requirement of Nisr L. monocytogenes.

Determination of bacteriocin cross-resistance.

DISCUSSION

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ACKNOWLEDGMENTS

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FOOTNOTES

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† Manuscript D-10974-2-97 of the New Jersey Agricultural Experiment Station.

REFERENCES

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

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