Pewarnaan Gram Metode Preston dan Morrel - SlideShare [PDF]

Apr 29, 2011 - Pewarnaan Gram Metode Preston dan Morrel• Mengidentifikasi 2 kelompok bakteri, yaitu : Bakteri Gram Pos

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Pewarnaan Gram Metode Preston dan Morrel 6,311 views

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Nur Latifah , Student at FMIPA-Kimia ITS Teknik PewarnaanPewarnaan SederhanaPewarnaan Bertingkat / Differensial Follow Published on Apr 29, 2011

Presentasi yang dibuat untuk memenuhi tugas KKPI (SMAKBo), tetapi Insyaallah ... Published in: Education 0 Comments 1 Like Statistics Notes

Full Name Comment goes here. 12 hours ago Delete Reply Block Are you sure you want to Yes No Your message goes here Pewarnaan SederhanaMenggunakan 1 macam zat warna.Tujuan : o Melihat bentuk morfologi selContoh : o Pewarnaan Positif o ...

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Oktia Zha at JYP 1 year ago No Downloads Views Total views 6,311 On SlideShare 0 From Embeds 0 Number of Embeds Menggunakan minimal 3 macam zat warna, yaitu : Pewarna Primer Zat Pemucat Pewarna Tandingan ... 10 Actions Shares 0 Downloads 177 Comments 0 Likes 1 Embeds 0 No embeds No notes for slide

Pewarnaan Gram Metode Preston dan Morrel 1. 2. 3. 4.

1. What will we discuss. . ? Pewarnaan Gram Teknik Pewarnaan Pewarnaan Bakteri 2. Teknik PewarnaanPewarnaan SederhanaPewarnaan Bertingkat / Differensial 3. Pewarnaan SederhanaMenggunakan 1 macam zat warna.Tujuan : o Melihat bentuk morfologi selContoh : o Pewarnaan Positif o Pewarnaan Negatif 4. Menggunakan minimal 3 macam zat warna, yaitu : Pewarna Primer Zat Pemucat Pewarna Tandingan Tujuan : Pemisahan kelompok Penampakan StrukturContoh : Pewarnaan Gram Pewarnaan Bakteri Tahan Asam Pewarnaan Kapsul Pewarnaan Spora Pewarnaan Flagella Pewarnaan Inti / Bahan Inti 5. 5. Pewarnaan Gram MekanismeBagan Kerja Pewarnaan 6. 6. Pewarnaan Gram Metode Preston dan Morrel• Mengidentifikasi 2 kelompok bakteri, yaitu : Bakteri Gram Positif Bakteri Gram Negatif• Hal ini sesuai dengan nama Pewarnaan Gram MekanismeBagan Kerja Pewarnaan penemunya, yaitu Dr. Christian Gram. 7. 7. Prinsip Bakteri Gram Positif : • Mengikat persenyawaan iodine kompleks (kristal violet dan lugol) Bakteri Gram Negatif : • Melepaskan persenyawaan iodine kompleks saat diberi zat pemucat (aseton : iodine / 3:1) • Mengikat zat warna kedua (karbol fuchsin) 8. 8. TujuanMempelajari landasan teori dan kimiawi dari prosedur pewarnaan differensial.Mempelajari landasan kimiawi pewarnaan gram.Mempelajari prosedur yang dapat membedakan kelompok bakteri Gram positif dan Gram negatif 9. 9. Alat-alat yang digunakan : Bahan-bahan yang Kaca alas datar digunakan : Pencil glass Alkohol 70 % Spiritus Kapas Ose Suspensi bakteri 24 jam Tabung reaksi LF Sumbat Kristal violet Rak tabung reaksi Air suling Bak pewarna Lugol Labu semprot Aseton : Iodin (3:1) Piala gelas Karbol fuchsin Mikroskop Kertas saring Minyak imersi 10. 10. Bagan Kerja>> 0 >> 1 >> 2 >> 3 >> 4 >> 11. 11. Mekanisme Pewarnaan 12. 12. >> 0 >> 1 >> 2 >> 3 >> 4 >> 13. 13. >> 0 >> 1 >> 2 >> 3 >> 4 >> 14. 14. • Pada langkah pemucatan• Pada pemberian pereaksi• Kultur yang dipakai umurnya tidak lebih dari 24 jam Hal-hal yang Harus Diperhatikan ! ! 15. 15. Contoh Bakteri Bakteri Gram Positif : Bakteri Gram Negatif :Streptococcus sp. Escherichia coli Staphylococcus aureus

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