Plenary Sessions [PDF]

Plenary Sessions

1

Room “Juarez II” Sunday 17:00-18:00

Chair Monday 9:00-9:50

9:50-10:40

Chair Tuesday 9:00-9:50 9:50-10:40

Chair Wednesday 9:00-9:50 9:50-10:40

Chair Thursday 9:00-9:50

Chair 9:50-10:40 Chair

EVOLUTION OF AN ARSENAL: DIVERSIFICATION OF THE REPTILE VENOM SYSTEM B.G. Fry A. Alagón NOVEL BINDING PARTNERS AND NOVEL SUBSTRATES OF THE PIII SNAKE VENOM METALLOPROTEINSES: INSIGHTS INTO THE PATHOBIOLOGY OF THE HEMORRHAGIC TOXINS J. W. Fox, J. Kim, M. Kock, R.Wagener, A. Pinto, J. M. Gutierrez, T. Escalante and S.M.T. Serrano SNAKE VENOM METALLOPROTEINASES AND MECHANISMS INVOLVED IN HEMORRHAGIC ACTIVITY A. M. Moura-da-Silva J.M. Gutiérrez INSIGHTS INTO THE MECHANISMS OF LOCAL AND SYSTEMIC MYOTOXICITY INDUCED BY SNAKE VENOM PHOSPHOLIPASES A2 J. M. Gutiérrez CYANOBACTERIAL CYANOTOXINS: STRUCTURE, OCCURRENCE, MONITORING AND MANAGEMENT OPTIONS AND THE DEVELOPMENT OF HUMAN RISK ASSESSMENT MODELS W. W. Carmichael R. Stock ALKALOIDAL TOXINS FROM SPIDER VENOMS: ETHNOBIOLOGY AND NEUROTOXICITY M. S. Palma THE FIFTH GENERATION OF ANTI VENOMS: HUMAN RECOMBINANT ANTIBODY LIBRARIES AS THE SOURCE B. Becerril and L. Riaño-Umbarila G. Corzo Conus PEPTIDES: DRUG DEVELOPMENT, AND THE GENERATION OF BIODIVERSITY B. M. Olivera E. Heimer Intellectual Property Meeting J. Paniagua, A.M. Chudzinski-Tavassi and J.L. Solleiro J. Paniagua,

2

Simposia Sessions

12

Monday 22nd Room Symposium 1 “Juarez III” (Toxin Proteomics) 11:00-12:15 STUDIES ON THE IN VIVO/IN VITRO EFFECTS OF SNAKE VENOM METALLOPROTEINASES BY PROTEOMIC APPROACHES S.M.T. Serrano, A.F. Paes-Leme, A.K. Oliveira, M.T. Assakura, A.C.M. Camargo, J.W. Fox NOVEL TOXINS FROM SNAKE VENOMS R. M. Kini PROTEOMICS OF THE NEUROTOXIC FRACTIONS FROM THE SEA ANEMONES BUNODOSOMA CANGICUM AND BUNODOSOMA CAISSARUM VENOMS: NOVEL PEPTIDES BELONGING TO NEW CLASS OF TOXINS K. Konno, A.J. Zaharenko, W.A. Ferreira-Jr., J.S. Oliveira, M. Richardson, D.C. Pimenta, F.C.V. Portaro, J.C. Freitas Chair

Dr. S. Serrano

Room Symposium 2 “Juarez III” (Antiinflammatory and immunomodulatory effects of animal toxins) 12:15-13:30 ANTI-INFLAMMATORY, ANTI-TUMORAL AND ANALGESIC PROPERTIES OF CROTOXIN, THE MAIN NEUROTOXIC COMPONENT OF THE SOUTH AMERICAN RATTLESANKE Crotalus durissus terrificus Cury Y, BIOLOGICAL PROPERTIES OF TWO RECOMBINANT MUTANTS OF Loxosceles boneti SPHINGOMYELINASE D R. Sánchez-López, B. Ramos-Cerrillo, A. Olvera, L. Olguín-Pérez, G. Saab, A. Pérez-Torres and A. Alagón COBRA VENOM FACTOR: FROM A QUIRK OF NATURE TO A NEW CLASS OF ANTI-INFLAMMATORY AGENTS C.W. Vogel and D. C. Fritzinger Chair

Dr. Y. Cury

13

Thursday 25th Room Symposium 5 “Juarez III” (Microbial and Plant Insecticidal Toxins) 11:00-12:15 MECHANISM OF ACTION OF CRY PROTEINS FROM BACILLUS THURINGIENSIS N. Jimenez-Juárez, I. Gómez, I. Arenas, L. Pardo, C. Muñoz-Garay, S.S. Gill2, B. Tabashnik3, M. Soberón and A. Bravo. DNA SHUFFLING APPLIED TO CREATE NEW Bt TOXINS Grossi-de-sa, M. F, Silva, M.C.M., Ramos, H.B, Firmino, A. A. P., Dias, S. C., Oliveira, G.R. BACTERIAL AND PLANT UREASES AS MULTIFUNCTIONAL TOXIC PROTEINS C. R. Carlini Chair

Dr. C. Carlini

Room Symposium 6 “Juarez III” (Marine Toxins) 12:15-13:30 HYDROXYPROLINE IN CONOTOXINS: IMPLICATIONS IN FOLDING AND ACTIVITY E. Lopez Vera, G. Bulaj, B.M. Olivera BIOCHEMICAL AND PHARMACOLOGICAL STUDIES OF THE “FIRE CORAL” MILLEPORA COMPLANATA A. Rojas PHARMACOLOGICAL CHARACTERIZATION OF SEA ANEMONE TOXINS COLLECTED FROM CUBAN SHORES. A. Garateix, E. Salceda, O. López, A. Rodríguez, E. Soto Chair

Dr. E. Heimer

27

Oral Sessions

34

Monday 22nd Room “Juarez II” 15:30-15:50

15:50-16:10

16:10-16:30 16:30-16:50

Chair

SELECTIVE ANTINEOPLASIC ACTIVITY FROM Tityus discrepans SCORPION VENOM PEPTIDES BY APOPTOSIS VIA FasL EXPRESSION G. D'Suze, A. Rosales, V. Salazar, C. Sevcik, J. Brazón CROTALUS VENOM AS SOURCE OF TEMPLATES FOR THERAPEUTIC AND DIAGNOSTIC CANCER TOOL M.A. Soares; P.B. Pujatti; L.R.V. Antonelli; P. A. Auler; G.D. Cassali; C.L. Fortes Dias; C.D.C. Olortegui; C. Simal; R.G. Santos1 BIOLOGICAL ACTIVITIES OF VITALIUS DUBIUS VENOM AND SEMIPURIFIED FRACTIONS T.A.A. Rocha-e-Silva, F. R. Báu, E. Antunes, L. Rodrigues-Simioni, S. Hyslop TEACHING TOXINOLOGY IN BRAZIL – CEVAP’S MODEL Ferreira Junior, RS, Barraviera, B. Dr. G. D'Suze

Room “Juarez III” 15:30-15:50 SNAKE VENOMICS OF CENTRAL AMERICAN SPECIES FROM THE ATROPOIDES AND BOTHRIECHIS GENERA J.J. Calvete, J. Escolano, Y. Angulo, B. Lomonte, J.M. Gutiérrez, L. Sanz 15:50-16:10 SNAKE VENOMICS OF THE SOUTH AND CENTRAL AMERICAN BUSHMASTERS. COMPARISON OF THE TOXIN COMPOSITION OF LACHESIS MUTA GATHERED FROM PROTEOMIC VERSUS TRANSCRIPTOMIC ANALYSIS L. Sanz, J. Escolano, M. Ferreti, M.J. Biscoglio, Y. Angulo, B. Lomonte, J.M. Gutiérrez, J.J. Calvete 16:10-16:30 SYNTHESIS, STORAGE AND DEPLOYMENT OF BIOLOGICAL WEAPONS BY VENOMOUS SNAKES S. P. Mackessy and L. M. Baxter 16:30-16:50 VENOM COMPOSITIONAL TRENDS AMONG RATTLESNAKES S. P. Mackessy Chair

Dr. J. Perez

35

Tuesday 23rd Room “Juarez II” 15:30-15:50 15:50-16:10 16:10-16:30

16:30-16:50

Chair

TRPV1 IS A KEY DETERMINANT IN MARINE INTOXICATIONS E. Cuypers, A. Yanagihara, J. D. Rainier, E. Karlsson, K. Shiomi, J. Tytgat LOW MOLECULAR WEIGHT COMPONENTS FROM SEA ANEMONE VENOMS: A NEGLECTED FIELD OF INVESTIGATION A. J. Zaharenko, W. A. Ferreira Jr., R. K. Aoki, K. Konno, J. C. de Freitas. DIFFERENTIAL FUNCTIONAL ACTIVITY AND ICHTHYOTOXICITY OF STICHOLYSINS, TWO PORE-FORMING PROTEINS PRODUCED BY THE SEA ANEMONE STICHODACTYLA HELIANTHUS D. Martinez, J. L. Ponce de Leon, C. Soto, E. Perez, F. Pazos, M. E. Lanio, Y. Cruz, R. Silveira, A. Vidal y andC. Alvarez BIODISTRIBUTION AND PHARMACOLOGICAL EFFECTS OF SCORPAENA PLUMIERI VENOM J. Soprani, S.G. Figueiredo, C. Simal, R.G. Santos Dr. J. Tytgat

Room “Juarez III” 15:30-15:50 CRYSTAL STRUCTURE OF A NOVEL MYOTOXIC ARG49 PHOSPHOLIPASE A2 HOMOLOGUE (ZHAOERMIATOXIN) FROM ZHAOERMIA MANGSHANENSIS VIPER VENOM M. T. Murakami, U. Kuch, J. R. Olivieri, D. Mebs and R. K. Arni 15:50-16:10 BIOLOGICAL AND STRUCTURAL CHARACTERIZATION OF NEW PLA2 FROM THE Bothrops marajoensis SNAKE VENOM L. A. Ponce-Soto, J. C. Novello and S. Marangoni 16:10-16:30 INSIGHTS INTO THE ROLE OF OLIGOMERIC STATE ON THE BIOLOGICAL ACTIVITIES OF CROTOXIN: CRYSTAL STRUCTURE OF A TETRAMERIC PHOSPHOLIPASE A2 FORMED BY TWO ISOFORMS OF CROTOXIN B FROM CROTALUS DURISSUS TERRIFICUS D.P Marchi-Salvador, L.C. Corrêa, A.J. Magro, C.Z. Oliveira, A.M. Soares, M.R.M Fontes 16:30-16:50 BAP1 A METALLOPROTEINASE ISOLATED FROM Bothrops asper SNAKE VENOM INDUCES JOINT HYPERNOCICEPTION THROUGH TNF-α AND PGE2-DEPENDENT MECHANISMS T. CFP, Fernandes CM, Leite ACRM, Gutiérrez JM, Rocha FA Chair

Dr. D. Mebs

44

Thursday 25th Room “Juarez II” 15:30-15:50 15:50-16:10

16:10-16:30

16:30-16:50

16:50-17:10

Chair Room “Juarez III” 15:30-15:50

15:50-16:10

16:10-16:30 16:30-16:50 16:50-17:10

Chairs

VENOMICS, THE VENOMOUS SYSTEMS GENOME PROJECT André Ménez, Reto Stöcklin, Dietrich Mebs VENTURING WITH THE MOLECULAR DIVERSITY OF THE VENOM OF CONE SNAILS: FROM SMALL CONOPEPTIDES TO CONOPROTEINS Carolina Moller, Sanaz Dovell, Aldo Franco, Herminsul Cano, Kymberly Sparling, Frank Marí HIGH-AFFINITY POTENTIATION AND LOW-AFFINITY INHIBITION OF NICOTINIC ACETYLCHOLINE RECEPTORS BY TWO α-CONOTOXINS FROM CONUS SPURIUS M.B. Aguilar, E. López-Vera, E. Schiavon, C. Marinzi, E. Ortiz, R. Restano Cassulini, C.V.F. Batista, L.D. Possani, E.P. Heimer de la Cotera, F. Peri, B. Becerril, E. Wanke THE PREPORE-OLIGOMER IS AN OBLIGATE INTERMEDIATE IN THE CELL DEATH INDUCED BY Bacillus thuringiensis Cry1Ab TOXIN INSECT LARVAE Nuria Jiménez-Juárez, Carlos Muñoz-Garay, Isabel Gómez, Sarjeet S. Gill, Mario Soberón, and Alejandra Bravo. IMMUNOCHEMICAL CHARACTERIZATION OF M. laticollaris CORAL SNAKE VENOM Carbajal-Saucedo A., Benard-Valle M., López-Vera E, Mendoza-Hernández G, Alagón A Dr. M.B. Aguilar

COMPARATIVE ACTION OF SPECIFIC FAB AND FAB’2 ANTIBODIES ON THE PHARMACOKINETICS OF VIPER AND SCORPION VENOMS DURING ENVENOMATIONS C. Bon ON THE PRESENCE OF ANTIBODIES AGAINST BOVINE, EQUINE AND POULTRY IMMUNOGLOBULINS IN HUMAN IGG PREPARATIONS, AND ITS IMPLICATIONS ON ANTIVENOM PRODUCTION C. Sevcik, Patricia Díaz, G. D'Suze SCORPION VENOM COMPLEXITY FRACTAL ANALYSIS. ITS RELEVANCE FOR COMPARING VENOMS AND ANTIVENOM PRODUCTION C. Sevcik, G. D'Suze EGG YOLK ANTIBODY ANTI-AFRICAN COBRA VENOMS Thereza L. Kipnis , Cláudia L. Silva, Claudia M. C. Almeida, Humberto P. Couto, Rita. C. M. Escocard, David. Gitirana, Lynna P. Sentinelli, Wilmar Dias da Silva POLYVALENT F(ab´)2 ANTIBODIES ANTI-BITIS AND ANTI_NAJA SNAKE VENOMS Wilmar Dias da Silva, Cláudia L. Silva, Juliana A. Silva, Thereza L. Kipnis Drs. C. Sevcik and C. Bon

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Poster Sessions

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Monday 22nd 001 PRELIMINARY STUDY ON THE INCIDENCE OF SNAKEBITES IN BOLIVIA J.-P. Chippaux, J. R. Postigo, G. C. Onofre Arce 002 A MOUSE EAR MODEL TO STUDY THE ALTERATIONS INDUCED BY SNAKE VENOM TOXINS ON SKIN AND MICROVASCULATURE. Natalia Jiménez A, Alexandra Rucavado R1, José María Gutiérrez G. 003 EFFECTS OF THE NATRIURETIC PEPTIDE ISOLATED FROM Crotalus durissus cascavella IN RENAL PERFUSION SYSTEM Evangelista, J.S.A.M.; Alves,R.S; Evangelista, J.J.F.; Toyama, M.H; Evangelista, I.L.; Jorge, R.J.B; Jorge, A.R.C.; Maia, D. G.; Moraes, M.E.A., Monteiro, H.S.A 004 PLATELET ACTIVE COMPONENTS FROM Agkistrodon blomhoffii ussuriensis SNAKE VENOM V.L. Karbovskyy, A.N. Savchuk, G.L. Volkov, M.E. Fadem. 005 THE EFFECT OF THE FRACTION OF Coluber ravergieri PHOSPHORYLATION OF MITOCHONDRIA IN RAT LIVER Kazakov I., Abubakirova M.E.

ON

THE

OXIDATIVE

006 NEUROTOXIC COMPONENET OF THE VENOM OF Coluber ravergieri OF THE FAMILY COLUBRIDAE Kazakov I. 007 THE EFFECT OF FRACTION OF Coluber ravergieri ON BILAYER LIPID MEMBRANES Abubakirova M.E. 008 IMMUNOCHEMICAL AND BIOLOGICAL CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST BaP1, A METALLOPROTEINASE FROM Bothrops asper SNAKE VENOM. G.G. Assumpção, C.R.F. Silveira, I. Tanjoni, E.L. Faquim-Mauro, A.M. Moura-da-Silva, J.M. Gutiérrez, A. Rucavado, H.A. Takehara, I. Fernandes 009 HEMOSTATIC ACTIVITIES of CRUDE VENOM AND FRACTIONS from Bothrops colombiensis snakes M.E. Girón, B.Guerrero, A.M. Salazar, I. Aguilar, J.C. Pérez, E.E. Sánchez, C.L.Arocha-Piñango, A.RodríguezAcosta. 010 THE ISOLATION OF A FIBRINOLYTIC ENZYME FROM THE VENEZUELAN RATTLESNAKE Crotalus durissus cumanensis VENOM I. Aguilar, S.Lucena, A.M. Salazar, B. Guerrero, M.E. Girón, E.E. Sánchez, J.C.Pérez, Rodríguez-Acosta. A. 011

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INHIBITION OF PLATELET ADHESION INDUCED BY A DISINTEGRIN FROM THE VENEZUELAN SNAKE Crotalus durissus cumanensis VENOM B.Guerrero, M. Da Silva, I.Aguilar, S.Lucena, A.M.Salazar, M.E. Girón, E.E.Sánchez, M. García, A.RodríguezAcosta 012 HEMOSTATIC AND LETHAL INDIVIDUAL VENOM VARIABILITIES IN THE SOUTH AMERICAN RATTLESNAKE Crotalus durissus cumanensis A.M. Salazar, I. Aguilar, B. Guerrero, M.E. Girón, J.C. Pérez, E.E. Sánchez, A. Rodríguez-Acosta 013 PURIFICATION AND CHARACTERIZATION OF A HEMORRHAGIC FRACTION FROM THE VENOM OF LANSBERG'S HOGNOSE PIT VIPER (Porthidium lansbergii hutmanni) A. Estrella, M.E. Girón, I. Aguilar, E.E. Sánchez, J.C. Pérez, L.F. Navarrete, A.Rodríguez-Acosta 014 EFFECTS OF LANSBERG'S HOGNOSE PIT VIPERS (Porthidium Lansbergii hutmanni) VENOM ON RENAL ULTRASTRUCTURE IN EXPERIMENTAL MICE A.M. Vargas, H.J. Finol, H. Scannone, I. Fernández, G. Guerrero, M. Cepeda, M. E. Pineda, A. RodríguezAcosta. 015 BIOCHEMICAL AND TOXINOLOGICAL CHARACTERIZATION OF THE VENEZUELAN RATTLESNAKE Crotalus pifanorum VENOM I. Fernandez, A. M. Vargas, G. Guerrero, M. Cepeda, M. E. Pineda, A. Rodriguez-Acosta, H. Scannone. 016 HEMOSTATIC, HEMOLYTIC AND INFLAMMATORY EDEMA ACTIVITIES OF VENEZUELAN RATTLESNAKE Crotalus pifanorum VENOM. H. Scannone, I. Fernandez, G. Guerrero, M. Cepeda, M. E. Pineda, A. Rodríguez-Acosta, A. M. Vargas

THE

017 BIOLOGICAL AND STRUCTURAL CHARACTERIZATION OF TWO PLA2 ISOFORMS Cdc-9 AND Cdc-10 FROM Crotalus durissus cumanensis SNAKE VENOM Frey Francisco Romero-Vargas, Luis Alberto Ponce-Soto, Daniel Martins, José Camillo Novello and Sergio Marangoni 018 ABILITY OF HIGH- AND LOW-MOLECULAR WEIGHT FUCOIDAN TO PREVENT MUSCLE NECROSIS INDUCED BY MYOTOXIN II AND VENOM OF THE SNAKE Bothrops asper: Karol Azofeifa, Yamileth Angulo, Bruno Lomonte 019 COMPARATIVE CHARACTERIZATION OF THE THROMBIN-LIKE ENZYMES DERIVED FROM THE SNAKE VENOMS OF Agkistrodon halys halys AND Agkistrodon blomhoffi ussuriensis S.P. Gavrylyuk, V. Karbovskyy, Ie. Krasnobryzha 020 IDENTIFICATION OF CONTINUOUS INTERACTION SITES IN PLA2- PROTEIN COMPLEXES BY PEPTIDE ARRAYS Roberta M. M. Santos, Carlos Chávez-Olórtegui, Franck Molina, Claude Granier, Consuelo L. Fortes- Dias 021

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ANTIHEMORRHAGIC ACTIVITY OF OPOSSUM (Didelphis marsupialis) SERUM FRACTION AGAINST VENEZUELAN SNAKE Porthidium lansbergii hutmanni VENOM M. E. Pineda, A. M. Vargas, I. Fernandez, A. Rodríguez-Acosta, H. Scannone. 022 EFFECT OF Bothrops asper VENOM IN LYMPHATIC VESSELS IN MOUSE MESENTERY Javier Mora, Rodrigo Mora, José María Gutiérrez 023 STRUCTURAL AND FUNCTIONAL DIFFERENCES BETWEEN P-I SNAKE VENOM METALLOPROTEINASES WITH DIFFERENT HEMORRHAGIC POTENCIES T.Escalante, M. Richardson, E.F. Sanchez, B. Lomonte, J.W. Fox, J.M. Gutiérrez 024 EVIDENCE OF RAPID PLASMA MEMBRANE DAMAGE EXTRACELLULAR CALCIUM IN THE CYTOTOXICITY INDUCED Y.Angulo, J.C., Villalobos, R. Mora, B. Lomonte, J.M. Gutiérrez

AND

DUAL

ROLE

FOR

025 STRUCTURE-FUNCTION CORRELATIONS OF THE RECOMBINANT SNAKE VENOM DISINTEGRIN JERDOSTATIN R.J. Carbajo, L. Sanz, S. Mosulén, A. Pineda-Lucena, J.J. Calvete, 026 BNP1, A NOVEL P-I METALLOPROTEINASE FROM Bothrops neuwiedi VENOM: BIOLOGICAL EFFECTS BENCHMARKING RELATIVELY TO JARARHAGIN, A P-III SVMP. C. Baldo, I. Tanjoni, I.R. León, I.F.C. Batista, M.S. Della-Casa, P.B. Clissa, R. Weinlich, M. Lopes-Ferreira, I. Lebrun, G.P. Amarante-Mendes, V.M. Rodrigues, J. Perales, R.H. Valente, A. M. Moura-da-Silva 027 ISOLATION OF A NEW BASIC PLA2 FROM Bothrops brazili PARTIAL BIOCHEMICAL AND BIOLOGICAL CHARACTERIZATION Salomón Huncahuire-Vega, Luis Alberto Ponce-Soto and Sergio Marangoni 028 PURIFICATION AND PARTIAL CHARACTERIZATION OF A SERINE PROTEASE WITH CHARACTER THROMBIN LIKE (TLBm) FROM Bothrops marajoensis SNAKE VENOM Vilca-Quispe, A.; Ponce-Soto, L.A.; Novello, J.C. and Marangoni, S. 029 EVOLUTION OF DISINTEGRIN GENE FAMILIES P. Juárez, I. Comas, F. González-Candelas, J.J. Calvete 030 PURIFICATION AND PARTIAL CHARACTERIZATION OF A HEMORRAGIC METALLOPROTEINASE BmajMP-I FROM Bothrops marajoensis SNAKE VENOM Frank Denis, Torres-Huaco, Luis Alberto, Ponce-Soto, José Camillo Novello, and Sergio Marangoni 031 BIOCHEMICAL CHARACTERIZATION OF A CYTOTOXIC L-AMINO ACID OXIDASE FROM Bothrops atrox SNAKE VENOM WITH PLATELET AGGREGATION INDUCING R. M. Alves, G. A. A. Passos, H. H. Paiva, E. P. M. Franqueiro, A. C. O. Cintra, J. J. Franco, A. L. Fuly, J. C. Rosa, M. D. Baruffi, S. V. Sampaio.

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032 NEUTRALIZATION OF TWO NORTH AMERICAN CORAL SNAKE VENOMS WITH UNITED STATES AND MEXICAN ANTIVENOMS Elda E. Sánchez, Juan C. Lopez-Johnston, Alexis Rodríguez-Acosta, John C. Pérez 033 EFFECTS OF PHOSPHOLIPASE A2 ISOLATED FROM Bothrops asper AND Crotalus durissus terrificus SNAKE VENOM ON EXPRESSION OF CYCLOOXYGENASES AND RELEASE OF PROSTAGLANDINS. V. Moreira; P. Souto; S.R., Zamunér, J.M. Gutiérrez; C.F.P., Teixeira 034 EFFECT OF LOW POWER LASER THERAPY IN THE MYONECROSIS INDUCED BY Bothrops jararacussu SNAKE VENOM. Barbosa, A. M.; Zamuner, S. R,; Villaverde, A. G. J. B; Guimarães-Souza, L.; Fernandes, C. M, Munin, E, Cogo, J. C. 035 EFFECT OF LOW LASER THERAPY ON EDEMA INDUCED BY Bothrops fonsecai SNAKE VENOM Guimarães-Souza, L, Barbosa, A. M, Cogo, J. C, Zamuner, S. R. 036 A NEW NON-HEMORRHAGIC METALLOPROTEASE FROM Bothrops jararacussu SNAKE VENOM Silvana Marcussi, Clayton Z. Oliveira, Maurício V. Mazzi, Danilo L. Menaldo, André L. Fuly, Angelo J. Magro, Marcos R. M. Fontes, José R. Giglio, Suely V. Sampaio, Andreimar M. Soares 037 EXPLORING THE VARIABILITY OF Bothrops jararaca VENOM BY PROTEOMIC ANALYSIS A. Zelanis; C. Gois; M.C. Menezes, A.C.M.Camargo, M.F.D. Furtado, P.L. Ho, S.M.T. Serrano 038 ACTIVATION OF LEUCOCYTE ROLLING BY THE NON-CATALYTIC DOMAINS OF HF3. M.C. Menezes, A.F. Paes-Leme, F.M. Bruni, R.L. Melo, C. Lima, M. Lopes-Ferreira, A.C.M. Camargo, J.W. Fox, S.M.T. Serrano 039 CROTAMINE INHIBITS PREFERENTIALY FAST TWITCHING MUSCLES BUT IS INACTIVE ON SODIUM CHANNELS C. T. Rizzi, J. L. Carvalho-de-Souza, E. Schiavon, A. C. Cassola, E. Wanke, L. R. P. Troncone. 040 INTERACTION OF P-I AND P-III SNAKE VENOM METALLOPROTEINASES WITH PLASMA AND EXTRACELLULAR MATRIX PROTEINS A.K. Oliveira, M.T. Assakura, A.C.M. Camargo, J.W. Fox2, S.M.T. Serrano 041 APOPTOSIS AND CELL CYCLE ARREST INDUCED BY SNAKE VENOM METALLOPROTEINASE BAP1 ON DIFFERENT CELL LINES O. Brenes, E. Muñóz, R. Roldan-Rodríguez, C. Díaz 042

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SEX DIFFERENCES IN THE ANALGESIC EFFECT OF CROTALPHINE, A PEPTIDE OBTAINED FROM Crotalus durissus terrificus SNAKE VENOM (CDTV). Cury Y, Britto L, Picolo G, Anselmo-Franci J 043 CHARACTERIZATION OF A NEW POLYVALENT ANTIVENOM (Antivipmyn Africa®) FOR USE IN SUB-SAHARAN AFRICA. B. Ramos-Cerrillo, A. Casasola, A. de Roodt, J. Paniagua-Solís, A. Alagón and R.P. Stock. 044 NEUROMUSCULAR BLOCKADE AND PLA2 ACTIVITY OF Micrurus altirostris (URUGUAYAN CORAL SNAKE) VENOM Abreu, V.A., Leite, G.B., Borja-Oliveira, C.R., Hyslop, S., Furtado, M.F., Rodrigues-Simioni, L.. 045 ANTIGENIC KINSHIP AND PARASPECIFIC PROTECTION IN Naja GENUS, A. Casasola, B. Ramos-Cerrillo, A. Carbajal-Saucedo, A. Alagon1, R.P. Stock. 046 NEW CONCEPTS FOR DRUG DISCOVERY IN SNAKE VENOMS Jay W. Fox, Bojan Dragulev, Alyson Prorock, Yongde Bao, Antonio Camargo, and Solange M.T. Serrano 047 ABILITY OF A LOW CONCENTRATION OF HEPARIN TO NEUTRALIZE THE NEUROMUSCULAR AND MYOTOXIC ACTIVITIES OF Bothrops jararacussu VENOM AND BOTHROPSTOXIN-I Rostelato-Ferreira, S., Cintra, A.C.O., Cruz-Höfling, M.A., Rodrigues-Simioni, L., Oshima-Franco, Y. 048 LOW MOLECULAR WEIGHT FRACTION FROM Bothrops jararaca VENOM PROMOTED DISRUPTION OF SEMINIFEROUS EPITHELIUM MICE BY THE NITRIC OXIDE PRODUCTION PATHWAY. Silva C.A., Gilio J.M., Pannocchia M.A., Portaro F.C.V., Fernandes B.L. and Camargo A.C.M. 049 IMMUNE RESPONSE OF DOGS IMMUNIZED WITH INACTIVATED BOTHROPS VENOM A.R. de Roodt, D. Pazos, L.C. Lanari, M.E. Gonzalez, D.C. Guilleron, N.C. Liria, R.D. Laskowicz, V.M. Manzanelli. 050 TOXIC CHARACTERISTICS OF THE VENOM OF Bothrops alternatus FROM ARGENTINE L.C. Lanari, M.E. Gonzalez, N.C. Liria, R.D. Laskowicz, V.M. Manzanelli, P.P. Galarce, J.A. Dolab, D.I.J. Herman, A.R. de Roodt. 051 SOME TOXIC AND BIOCHEMICAL CHARACTERISTICS ACTIVITIES OF BOTHROPS AMMODYTOIDES VENOM A.R. de Roodt, L.C. Lanari, H. Clement, V.M. Manzanelli, A. Alagon 052 INOTROPIC EFFECTS OF NEUROTOXIN CROTAMINE ON SKELETAL MUSCLE Hernandez-Oliveira, S. S, Borja-Oliveira C.R., Leite, G.B, Sanchez, E. F., Rodrigues-Simioni

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053 PURIFICATION AND NEUROMUSCULAR ACTIVITY OF A PHOSPOLIPASE A2 (bP-12) FROM Bothrops pauloensis SNAKE VENOM Randazzo-Moura P., Leite G.B., Ponce-Soto L., Marangoni S., Rodrigues-Simioni L. 054 NEUROTOXIC AND MYOTOXIC ACTIVITIES OF Bp-13 FROM Bothrops pauloensis SNAKE VENOM IN MOUSE PHRENIC NERVE-DIAPHRAGM PREPARATIONS Sucasaca-Monzón, G., Rocha, T., Cruz-Höfling, M.A., Ponce-Soto, L.A., Marangoni, S., Rodrigues-Simioni, L 055 EFFECTS ON PLATELET FUNCTION OF TWO HOMOLOGOUS BUT FUNCTIONALLY DIVERGENT SNAKE VENOM METALLOPROTEASES Ventura, J.S., Ramos, O.H.P., Moura-da-Silva AM, Chudzinski-Tavassi, A.M. 056 PREDICTIVE PROTEOME OF MICRURUS FRONTALIS VERSUS Micrurus corallinus VENOM Ramos-Guimarães, P.; Oliveira-Carvalho, A.L.; Correa-Neto, C.; Aguiar, A. S.; Melgarejo, A.R.; Leão, L.I.; Junqueira-de-Azevedo, I. L. M.; Ho, P.L.; Soares, MR; Zingali, R.B. 057 BIOLOGICAL & BIOCHEMICAL CHARACTERIZATION OF VENOMS OF THE NEW WORLD VINE SNAKES: Oxybelis aeneus AND Oxybelis fulgidus William H. Heyborne and Stephen P. Mackessy 058 VENOM ONTOGENY IN THE MEXICAN LANCE-HEADED RATTLESNAKE (Crotalus polystictus) Jamie Leroy, Stephen P. Mackessy, Estrella Mociño, Kirk Setzer and Robert W. Bryson 059 ANALYSIS OF VENOM FROM THE COLUBRID SNAKE Alsophis portoricensis (PUERTO RICAN RACER) Caroline Weldon and Stephen P. Mackessy 060 HUMAN HETEROPHILIC ANTIBODIES AGAINST EQUINE IMMUNOGLOBULINS: ASSESSMENT OF THEIR ROLE IN THE EARLY ADVERSE REACTIONS TO ANTIVENOM ADMINISTRATION G. León, Á. Segura, M. Herrera, R. Otero, F.O.S. França, K.C. Barbaro, J.L.C. Cardoso, F.H. Wen, C.R. Medeiros, J.C. Lopes, C.M.S.A. Malaque, B. Lomonte and J.M. Gutiérrez. 061 DEVELOPMENT AND VALIDATION OF AN IMMUNOASSAY FOR DETECTION AND QUANTITATION OF RATTLESNAKE (Crotalus atrox) VENOM IN HUMAN PLASMA SAMPLES Chávez-Méndez, A., Olguín-Pérez, L., Olvera-Rodríguez, F., Buelna-Romero Alma, García- Ubbelohde, W., Boyer, L., Alcántara-Pineda, A., Paniagua-Solís, J., Alagón, A. 062 BIOLOGICAL AND STRUCTURAL CHARACTERIZATION OF NEW PLA2 FROM THE Bothrops marajoensis SNAKE VENOM Luis Alberto Ponce-Soto, José Camillo Novello and Sergio Marangoni

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Tuesday 23rd 063 PROTEASES AND INHIBITORS FROM THE Amblyomma cajennense (ACARI: IXODIDAE) TICK’S SALIVA RELATED TO THE BLOOD COAGULATION AND CELL VIABILITY Simons, S.M.; Ventura, J.S.; Oliveira, D.G.L.; De-Sá-Júnior, P.L.; Silva-Ricci, M.E.; Chudzinski-Tavassi, A.M. 064 BIOACTIVE PEPTIDE SCREENING AND BIOLOGICAL EFFECTS OF THE SKIN SECRETION OF Phyllomedusa hypochondrilais. Conceição K, Konno K, Melo RL, Lima C, Bruni, FM, Pareja-Santos A, Antoniazzi MM, Jared C, Gilio JM, Silva CA, Conceição IM, Sciani J, Lopes-Ferreira M, Borella MI, Prezoto B, Camargo ACM, Pimenta DC 065 ANTIMICROBIAL ACTIVITY OF THE AMPHIPATHIC PEPTIDES ISOLATED FROM THE MEXICAN FROG Hyla eximia Oscar Villa-Hernández, Lorena Hernández-Orihuela, Fernando Zamudio Zuñiga, Rubén Castro-Franco, and Cesar V. F. Batista 066 DEVELOPMENT AND VALIDATION OF TWO SOLID-PHASE ENZYME IMMUNOASSAYS (ELISA) FOR QUANTITATION OF HORSE F(ab’)2-BASED AND SHEEP Fab-BASED ANTIVENOMS IN HUMAN PLASMA SAMPLES Olguín-Pérez, L., Chávez-Méndez, A., Olvera-Rodríguez, F., Buelna-Romero Alma, García- Ubbelohde, W., Boyer, L., Alcántara-Pineda, A., Paniagua-Solís, J., Alagón, A. 067 STUDY OF THE IMMUNIZATION PROCESS USING TOTAL AND 60Co-IRRADIATED VENOM FROM AFRICANIZED HONEY BEES (Apis mellifera) IN NANOSTRUCTURED MESOPOROUS SILICA (SBA-15) AS ADJUVANT. Ferreira Junior, RS, Barraviera, B, Sant’Anna, OA 068 ANTINOCICEPTIVE EFFECTS OF CRUDE AND DETOXIFIED BEE VENOM Guermani A., Delporte C., Miranda H.F., Spencer P., Hyslop. 069 CRUDE AND DETOXIFIED Apis mellifera VENOM. EVALUATION OF ANTINOCICEPTIVE ACTIVITY OF ITS FRACTIONS Guermani A., Delporte C., Miranda H.F., Spencer P., Hyslop S. 070 ACTION OF LONOMIN V (Lonomia achelous) ON FIBRONECTIN FUNCTIONAL PROPERTIES S.Lucena, A.Gil1,A.M.Salazar, C.L.Arocha-Piñango, A.Rodríguez-Acosta, B.Guerrero. 071 INHIBITION OF PLATELET AGGREGATION INDUCED BY Lonomia obliqua VENOM Carrijo-Carvalho LC, Oliva DP, Ventura JS, Seibert CS, Chudzinski-Tavassi AM 072 Lonomia obliqua PROCOAGULANT PROTEINS COULD BE CLASSIFIED AS NEW COAGULATION ACTIVATORS

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Ana Marisa Chudzinski-Tavassi, Linda Christian Carrijo-Carvalho, Oscar Henrique Pereira Ramos, Miryam Paola Alvarez Flores 073 EFFECT OF THE PLA2 ISOLATED FROM SEA ANEMONE Bunodosoma caissarum ON RENAL PERFUSION. Martins, R.D; Alves, R.S; Silva Neto, A.G; Toyama, M.H; Martins, A.M.C; Barbosa,P.S.F; Fonteles, M.C; Monteiro, H.S.A. 074 IN VITRO ANTI-Giardia lamblia ACTIVITY OF THE SCYPHOZOAN Linuche unguiculata (SWARTZ) FROM THE MEXICAN CARIBBEAN SEA J. L. Morales-Landa, O. Zapata-Pérez, J. Sánchez-Rodríguez, L. Segura-Puertas and R. Cedillo-Rivera 075 CRUDE VENOM FROM Stichodactyla helianthus INDUCES HEMOLYSIS AND SLIGHT PEROXIDATIVE DAMAGE IN RAT AND HUMAN ERYTHOCYTES, ANTIMICROBIAL ACTIVITY IN Aeromona hydrophila AND CITOTOXIC ACTIVITY IN Oreochromis niloticus H.I. Monroy-Estrada, J. Sánchez-Rodríguez and L. Segura-Puertas. 076 ISOLATION AND ELECTROPHYSIOLOGICAL CHARACTERIZATION FROM TOXINS ON MARINE SNAILS Conus californicus Bernáldez Johanna, Licea Alexei, Arellano O. Rogelio 077 ISOLATION AND PURIFICATION OF NEUROTOXIC COMPOUNDS BLOCKING IONIC CHANNELS AND INHIBITORS OF ACEYILCHOLINESTERASE FROM SEA ANEMONE Lebrunia danae (DUCHASSAING & MICHELOTTI, 1860) IN THE MEXICAN CARIBBEAN SEA K. Cruz-Vázquez, R. Arellano-Ostoa, J. L. Morales-Landa and J. Sánchez-Rodríguez 078 THE CYANONET EXERCISE: FROM SITUATION ASSESSMENT TO RECOMMENDATIONS G. A. Codd, S. M. F. O. Azevedo, S. N. Bagchi, M. D. Burch, W. W. Carmichael, W. R. Harding, T. Jurczak, K. Kaya, H. C. Utkilen, F. M. Young 079 SYSTEMIC PATHOLOGICAL ALTERATIONS CAUSED BY THE VENOM OF THE “FIRE CORAL” Millepora complanata IN MICE J. A. García-Arredondo, A. Rojas, M. A. Gallegos, M. García-Servín, C. Ibarra-Alvarado and E. P. Heimer de la Cotera 080 PARTIAL CHARACTERIZATION OF A NEW BIOACTIVE PEPTIDE ISOLATED FROM THE VENOM DUCTS OF Conus austini D. Hernández, A. Rojas, A. Torres, C. Ibarra-Alvarado, M. B. Aguilar, A. Falcón and E. P. Heimer de la Cotera 081 ANALGESIC EFFECT INDUCED BY THE COMPOUND LAS390 PURIFIED FROM SEA ANEMONE Bunodosoma cangicum VENOM Ferreira Jr. WA, Zaharenko AJ, Gutierrez VP, Konno K, Freitas JC, Picolo G and Cury Y. 082

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CYANOTOXINS BIOACUMULATION IN THE Hypophthalmicthys molitrix (SILVER CARP) OF THE PARANOÁ LAKE, DF, BRAZIL - A SANITARY RISK Oliveira, N. B. Schwartz C. A., Silva, L. P., Bloch Júnior, C., Pires Júnior, O. R. 083 PARTIAL PURIFICATION OF THE NEUROTOXINS FROM THE BOX JELLYFISH Carybdea marsupialis (LINNÉ, 1758) J. F. Lazcano-Pérez, J. Sánchez-Rodríguez and L. Segura-Puertas. 084 A RAPID, NON-DESTRUCTIVE, METHOD FOR SEPARATING THE ANIMAL FROM ITS SHELL: Conus cancellatus (Syn.: AUSTINI) AS AN EXAMPLE A. Falcón, M.B. Aguilar, E.P. Heimer 085 ISOLATION OF A FUNGUS RESISTANT TO CHROMIUM (VI) AND STUDY OF THE REMOTION OF THE METAL IN SOLUTION Diana Alvarado-Hernández, Juan F. Cárdenas-González, María de Guadalupe Moctezuma-Zárate e Ismael Acosta-Rodríguez 086 NEW PRODUCTS OF INDUSTRIAL INTEREST FROM MARINE MACRO AND MICROORGANISMS Roberto Nuñez, Maria Rodríguez, Eudays Ortíz, Erik Regalado, Julio Oramas, Olga Valdés, Gisela Marrero, Abilio Laguna. 087 MARINE ORGANISMS AS A SOURCE OF BIOACTIVE COMPOUNDS. Anoland Garateix, Roberto Menéndez, Miguel D. Fernández., Mirtha Llanio, Teidy García, Kenia Ruenes, Olga Valdés, Erik Regalado, Yasnay Hernández, Abilio Laguna 088 PURIFICATION AND CHARACTERIZATION OF BLACK BEAN LECTINS J.D. Salgado Telpalo, M. C. VALADEZ VEGA. 089 THE POLAR EXTRACT FROM Casearia sylvestris SW. IS THE MOST ACTIVE IN PREVENTING BOTHROPIC ENVENOMATION Oshima-Franco, Y, Cintra-Francischinelli, M, Gerenutti, M, Silva, M. G, Andréo-Filho, N, Leite, G. B, Dal Belo, C. A, Cintra, A. C. O, Cruz-Höfling, M. A, Rodrigues-Simioni, L. 5090 GENOTOXICIDAD Y CITOTOXICIDAD DE EXTRACTOS TOTALES DE LECTINAS OBTENIDOS DEL AMARANTO J. E. Maciel Rivera, M. C. Valadez Vega, R. Cariño Cortes 091 PHARMACOLOGICAL CHARACTERIZATION OF TRICOLORIN A ISOLATED FROM THE PLANT Ipomoea tricolor R. Hernández Matehuala, A. Vuelvas Solorzano, C. Ibarra-Alvarado, R. Pereda-Miranda, M. Fragoso-Serrano, and A. Rojas 092

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CLONING GENES FROM THE VENOMOUS GLAND OF THE Scolopendra viridis SAY González-Morales, L., Diego-Garcia E., Restano-Cassulini, R., Olamendi-Portugal, T., Zamudio, F.Z., Gutierrez, M.C. and Possani, L.D. 093 THE ROLE OF PRAZOSIN ON THE RENAL EFFECTS PROMOTED BY Tityus serrulatus VENOM. Monteiro, F.C.D.; Alves, R.S.; Martins, R.D.; Oliveira, I.M.S.; Aragão, F.O.F.; Jorge, A.R.C.; Havt, A.; Martins, A. M. C.; Toyama, M.H., Monteiro, H.S.A. 094 FROM CLONING TO THE PRODUCTION OF NEUTRALIZED SERA AGAINST SCORPION TOXINS OF FOREING COUNTRIES. Blanca I. Garcia-Gómez, Jorge Paniagua, Lourival Possani 095 AN EVOLUTIONARY GLANCE INTO A HYPERVARIABILITY CAUSING MECHANISMS A. Kozminsky-Atias, A. Bar-Shalom and N. Zilberberg

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096 ANTIBACTERIAL ACTIVITY OF SIX NOVEL PEPTIDES FROM SCORPION VENOM. A FLUORESCENT PROBE STUDY OF MICROBIAL MEMBRANE Na+ PERMEABILITY CHANGES. P. Díaz-Bello, G. D’Suze, V. Salazar, C. Sevcik, J. Fox 097 BIOCHEMICAL CHARACTERIZATION OF LOW MOLECULAR COMPONENTS FROM THE SCORPION VENOM OF Centruroides noxius HOFFMANN Hernández Aponte Cynthia A., Gurrola Briones Georgina, Diego-García Elia, Prestipino Gianfranco and Possani Lourival D. 098 DISCREPLASNIN A PLASMIN INHIBITOR PEPTIDE FROM Tityus discrepans SCORPION VENOM J. Brazón, B. Guerrero, G. D`Suze, C. Sevcik, C. Arocha-Piñango 099 ANTIFUNGAL ACTIVITY OF TsTX-I, A _-NEUROTOXIN FROM Tityus serrulatus SCORPION VENOM W.M. Santussi; S. Said, E. C. Arantes 100 ELETROCTROPHYSIOLOGICAL EFFECTS AND BIOCHEMICAL CHARACTERIZATION OF X-1, A NEW α-NEUROTOXIN FROM Tityus serrulatus VENOM C.T. Cologna; H.S.Selistre-de-Araújo; J.C. Rosa; W.A. Varanda; E. C. Arantes. 101 PROTEOMIC ANALYSIS AND TOXICITY OF Opisthacanthus cayaporum SCORPION VENOM: AMINO ACID SEQUENCE AND BIOLOGICAL ACTIVITY OF SOME PEPTIDES Elisabeth F. Schwartz; Thalita Camargos; César V. Batista; Fernando Zamudio; Luciano P. Silva; Carlos Bloch Jr.; Fábio Caixeta; Carlos A. Schwartz; Lourival D. Possani 102

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CAYAPORIN IS THE FIRST ANTIMICROBIAL PEPTIDE CHARACTERIZED FROM THE BRAZILIAN SCORPION Opisthacanthus cayaporum Silva, E.C.N.; Camargos, T.S.; Silva, L.P.; Bloch Jr., C.; Maranhão, A.Q.; Pereira, I.S.; Possani, L.D.; Schwartz, E.F. 103 CLONING AND PEPTIDE SEQUENCING OF A GAMMA-LIKE TOXIN FROM THE ARGENTINIAN SCORPION Tityus trivittatus. F. I. Coronas, E. Diego-Garcia, F. Zamudio, A. R. De Roodt, R. Restano-Cassulini, L. D. Possani 104 CHARACTERIZATION OF TWO NOVEL ERGTOXINS FROM THE VENOM OF THE SCORPION Centruroides elegans elegans T. Olamendi-Portugal, F. Zamudio, R. Restano-Cassulini, B. Becerril, L. D. Possani 105 SCORPINE AND βKTX-LIKE PEPTIDES FROM SCORPION VENOMS: GENES, PEPTIDES AND ACTIVITIES OF THE “ORPHAN” COMPONENTS E. Diego-Garcia, E. Schwartz, Y. Abdel-Mottaleb, R. C. Rodríguez de la Vega, A. R. De Roodt, Jan Tytgat and L. D. Possani 106 DIFFERENT IMMUNOCHEMICAL REACTIVITY OF FOUR ANTI-SCORPION ANTIVENOMS ON TELSON HOMOGENATES AND CRUDE VENOM OF Tityus trivittatus FROM ARGENTINE. M.E. Gonzalez, N.C. Liria, J. Estevez. A.R. de Roodt 107 RECOMBINANT EXPRESSION OF A FOUR DISULFIDE-BRIDGED SCORPION TOXIN THAT MODIFIES IONIC CURRENTS OF VOLTAGE-GATED SODIUM CHANNELS Estrada, G., Ortiz, E., Possani, L.D., Corzo, G. 108 Cn2, A POTENT TOXIN FROM Centruroides noxius HOFFMANN SCORPION VENOM: HETEROLOGOUS EXPRESSION AND FOLDING. Verónica Quintero-Hernández., Baltazar Becerril and Lourival D. Possani. 109 THERMODYNAMIC STABILITY VERSUS NEUTRALIZATION CAPACITY OF DIFFERENT FORMATS OF THE HUMAN RECOMBINANT ANTIBODY 6009F AGAINST TOXIN Cn2 AND WHOLE VENOM FROM THE SCORPION Centruroides noxius Víctor R. Juárez González, Lidia Riaño-Umbarila, Verónica Quintero-Hernández, Israel Alcántara-Recillas, Lourival D. Possani y Baltazar Becerril. 110 A SELECTIVE POTASSIUM CHANNEL BLOCKER OF Kv1.2 AND Kv1.3 FROM THE SOLUBLE VENOM OF THE SCORPION Centruroides suffusus suffusus Gerardo Corzo, Gerardo Pavel Espino, Ferenc Papp, Zoltan Varga, Rezso Gaspar, Gyorgy Panyi, Lourival D. Possani. 111 THE IMPORTANCE OF A PROLINE KINK IN AN ALPHA-HELICAL ANTIMICROBIAL PEPTIDE TOWARDS TOXICITY OF PATHOGENIC BACTERIA Elizabeth Aguilar, Mariel Adame1, Possani, L.D., Gerardo Corzo, Elba Villegas

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112 IMMUNOASSAY FOR THE DETECTION AND QUANTITATION OF Centruroides SCORPION VENOM IN HUMAN PLASMA SAMPLES Chávez-Méndez, A., Olguín-Pérez, L., Olvera-Rodríguez, F., Buelna-Romero Alma, García- Ubbelohde, W., Boyer, L., Alcántara-Pineda, A., Paniagua-Solís, J., Alagón, A. 113 Tityus asthenes SCORPION STINGS IN MUTATÁ, COLOMBIA: CLINICAL, EPIDEMIOLOGICAL AND TOXINOLOGIC ASPECTS Juan P. Gómez1,2, Juan C. Quintana2, Patricia Arbeláez3, Jorge Fernández4, Juan F. Silva4, Jacqueline Barona2, Juan C. Gutiérrez5, Rafael Otero1 114 AUTORHYTHMIC ACTIVITY IN THE CHICK BIVENTER CERVICIS MUSCLE CAUSED BY VENOM OF THE AUSTRALIAN THERAPHOSID SPIDER Phlogius crassipes V. Herzig, W.C. Hodgson 115 INTERSEXUAL VARIATIONS IN THE VENOM OF THE AUSTRALIAN THERAPHOSID SPIDER Coremiocnemis tropix V. Herzig, W.C. Hodgson 116 STRUCTURAL AND ULTRASTRUCTURAL ANALYSIS OF THE VENOM GLANDS OF THE ARMED SPIDER Phoneutria nigriventer (KEYSERLING, 1891) Luciana M. Silva, Ana C. C. Botelho, Paulo F. P. Pimenta, Consuelo L. Fortes-Dias 117 PENILE ERECTION INDUCED BY A SPIDER VENOM TOXIN: INTRACAVERNOSAL INJECTION AND HISTOLOGICAL FINDINGS K. R. M. Leite, E. Andrade1, F. Villanova, P. A. Borra, A. T. Ramos, F. C. Magnoli, L. Magalhães, L. R. P. Troncone, M. Srougi 118 PEPTIDE PROFILING BY MATRIX-ASSISTED LASER DESORPTION-IONIZATION TIME-OFFLIGHT MASS SPECTROMETRY OF THE Acanthoscurria atrox TARANTULA VENOM: EVIDENCES FOR SEX-LINKED VENOM VARIATION Caroline B. F. Mourão; Luciano P. Silva; Carlos Bloch Jr.; Elisabeth F. Schwartz 119 EXPRESSION OF RECOMBINANT α-LATROTOXIN FROM Latrodectus mactans USING THE INSECT CELL BACULOVIRUS SYSTEM M. Rodríguez, A. Alagón and R. Stock 120 EXPRESSION OF RECOMBINANT Brachypelma vagans HYALURONIDASE IN THE INSECT CELL BACULOVIRUS SYSTEM H. Clement, R. Sánchez-López, M. Rodríguez, A. Olvera, G. Odell and A. Alagón 121 Phoneutria nigriventer VENOM IMPAIRS EXPRESSION OF JUNCTIONAL PROTEINS IN BLOOD BRAIN BARRIER THROUGH PHOSPHATASES DEACTIVATION PATHWAY

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MA Cruz-Höfling, C Rapôso, K Fontana, H Aoyama, CV Ferreira, RR Sousa 122 THE SELECTIVE NNOS INHIBITION ANTICIPATES AND AGGRAVATES THE CLINICAL SIGNS OF Phoneutria nigriventer ENVENOMING M.A. Cruz-Höfling, C. Rapôso, K. Fontana 123 NEUTRALIZATION OF RECOMBINANT SPHINGOMYELINASE D FROM Loxosceles boneti, WITH VARIOUS PREPARATIONS DERIVED FROM HORSE HYPERIMMUNE SERUM. Jiménez-Martínez L., Vázquez, H. and Alagón C. A 124 PRODUCTION OF A CHIMERIC Fab ANTI-SPHINGOMYELINASE D FROM Loxosceles SPIDER VENOM C.L. Moctezuma, V. Quintero, B. Becerril, A. Olvera, A. Alagón 125 LyeTx, A NEW TOXIN FROM Lycosa erythrognatha WITH ANTIMICROBIAL ACTIVITY. Santos, D.M., Moreira, D.F.F., Richardson, M., Bemquerer, M.P; Galuppo, C.D., Farias, L.M; Carvalho, M.A.R.; Soares, B.M., Kohler, L.M., Cisalpino, P., De Maria, M., Pimenta, A.M.C., De Lima, M.E, 126 DETECTION AND QUANTITATION OF LOXOSCELES SPHINGOMYELINASE D (SMD) BY ELISA METHOD A. Olvera, H.M. Cardoso, F. Olvera, L.G. Medina, A. Buelna, A. Alagón 127 Polybia paulista MASTOPARAN AND CYTOKINE EXPRESSION IN SKELETAL MUSCLE T. Rocha, L.L.S. Barros, K. Fontana, B.M. de Souza, M.S. Palma, M.A. Cruz-Hofling 128 CHARACTERIZATION OF CgNa, A TYPE I TOXIN FROM THE GIANT SEA ANEMONE CONDYLACTIS GIGANTEA E. Salceda1, A. Garateix2, H. Salazar1, O. López1, A. Ortega1, R. Vega1, E. Soto1 129 GENE STRUCTURE OF HETERODIMERIC PHOSPHOLIPASES A2 FROM THE VENOM OF THE SCORPION Anuroctonus phaiodactylus Norma A. Valdez-Cruz1, Lorenzo Segovia1, Miguel Corona2, Lourival D. Possani

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