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Agarose Gel Electrophoresis - PowerPoint PPT Presentation By lalasa (/lalasa)

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Agarose Gel Electrophoresis (https://image2.slideserve.com/40827 50/agarose-gel-electrophoresisn.jpg) Gel Electrophoresis (https://image2.slideserve.com/4082750/slide2n.jpg) # . 200bp 50kb . , . .

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DNA Fingerprinting Supplies from Iowa State (/vaughn/dnaStudent fingerprintingPreparation DNA and Enzyme supplies-fromPreparation

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# , , , . # DNA RNA (agarose) , (polyacrylamide) . (https://image2.slideserve.com/4082750/slide3n.jpg)- [ sol-gel transition ]

# .

# (%) . DNA ladder (https://image2.slideserve.com/4082750/slide5n.jpg) http://www.axygenbio.com/ # Size marker size marker DNA . Features: Ready-to-use Includes bromphenol blue for ease of use Stable at room temperature (https://image2.slideserve.com/4082750/slide6n.jpg): (1) - (agarose) - 5x TAE buffer or TBE buffer - Ethidium bromide Stock 10mg/ml --> 0.5 µg/ml 25ml EtBr stock 2 µl .

(poly-glove) !

- Gel loading buffer(6X) - DNA Size marker - 100ml - , or (2-20 µl), yellowtip, (parafilm) 1 Gel tray - - UV transiluminator

# EtBr(: C21H20BrN3, :394.33) DNA . 2 DNA GC DNA RNA . DNA . . (2) (https://image2.slideserve.com/4082750/slide7n.jpg) ¬ 0.8% 100ml 25ml 1XTAE buffer 0.2g . ­ . ® 1-3 . Notice! ¯ 50-60 EtBr .

Notice! EtBr tip .

° Gel tray comb 30 gel . ± 0.5XTAE buffer . ² (well) tank “–” (DNA – + .) 0.5X TAE 3mm . (https://image2.slideserve.com/4082750/slide8n.jpg) ³ loading buffer 1 µL sample 5 µL (elution buffer , 1:4) well( well 20 µL, well 60 µL ) loading well DNA size marker 3 µL loading . ´ [black(-), red(+)] 30 100V . µ bromophenol blue 2/3 . UV transilluminator

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# , , pH - . Õ . , carageenan, , methylcellulose .

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# pcDNA 6B size: 5130bp (https://image2.slideserve.com/4082750/slide9n.jpg) # pcDNA 6B EGFP-EcR size: 7724bp

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