Preparing Buffers and Buffer Capacity - Westminster College

Loading...
PREPARING BUFFERS AND BUFFER CAPACITY LAB From Juniata College, Science in Motion

Westminster College

INTRODUCTION A buffer solution is one that is resistant to change in pH when small amounts of strong acid or base are added. For example, when 0.01 mole of strong acid or base are added to distilled water, the pH drops to 2 with the acid and rises to 12 with the base. If the same amount of acid or base is added to an acetic acid – sodium acetate buffer, the pH may only change a fraction of a unit. Buffers are important in many areas of chemistry. When the pH must be controlled during the course of a reaction, the solutions are often buffered. This is often the case in biochemistry when enzymes or proteins are being studied. Our blood is buffered to a pH of 7.4. Variations of a few tenths of a pH unit can cause illness or death. Acidosis is the condition when pH drops too low. Alkalosis results when the pH is higher than normal. Two species are required in a buffer solution. One is capable of reacting with –OH +

and the other will react with H O . The two species must not react with each other. Many 3

buffers are prepared by combining a weak acid and its conjugate (acetic acid and sodium acetate) or a weak base and its conjugate (ammonia and ammonium chloride). In general, the pH range in which a buffer solution is effective is +/- one pH unit on either side of the pKa. The Henderson–Hasselbalch provides the information needed to prepare a buffer. Ph = PkA + log

[conjugatebase] [ weakacid ]

There is a limit to the amount of acid or base that can be added to a buffer solution before one of the components is used up. This limit is called the buffer capacity and is defined as the moles of acid or base necessary to change the pH of one liter of solution by one unit. Buffer Capacity = (number of moles of -OH or H3O+ added) (pH change)(volume of buffer in L) In this experiment, the Henderson-Hasselbalch equation will be used to determine the amount of acetic acid and sodium acetate required to prepare a series of buffer solutions. Once the buffer solutions have been prepared, their buffer capacity will be determined.

Westminster College SIM

Page 1

Preparing Buffers and Buffer Capacity

PURPOSE The purpose of this experiment is to prepare buffer solutions and to determine their buffer capacity.

EQUIPMENT/MATERIALS acetic acid (0.10, 0.30, 0.50M) sodium acetate buret clamp standard buffer solution (pH 4 & 7) 100 mL graduated cylinder

NaOH 2 burets pH probe, LabQuest magnetic stirrer (if available) 250 mL beaker

SAFETY • Always wear an apron and goggles in the lab.

• Report any spills so they may be cleaned up.

Westminster College SIM

Page 2

Preparing Buffers and Buffer Capacity

PROCEDURE 1. Before preparing the buffer solutions, you must determine the amount of acetic acid -5

and sodium acetate required. The Ka of acetic acid is 1.8 X 10 . You will be using one of the provided acetic acid solutions (0.1M, 0.30 M, or 0.50M). Your instructor will assign one of these solutions for your group.

a. Calculate the number of moles of acetic acid present in 100 Ml of your assigned acetic acid solution.

b. If equal moles of sodium acetate and acetic acid are required to make an effective buffer, how many moles of sodium acetate need to be added to your acetic acid solutions?

c. Calculate the number of grams of sodium acetate needed using its molar mass.

Westminster College SIM

Page 3

Preparing Buffers and Buffer Capacity

2. Check your calculations with your instructor before proceeding.

a. In a graduated cylinder measure 100 mL of your assigned acetic acid solution and transfer it to a 250 mL beaker. b. Weigh out the correct mass of sodium acetate and dissolve it in the acetic acid solution to make your buffer.

3. Attach pH probe to LabQuest. 4. Measure the pH of the buffer solution that your group prepared. Record the value in your data table. 5. Set up two burets, buret clamp, and ring stand (see Figure 1). Rinse and fill one buret with 0.100 M NaOH solution. Rinse and fill the second buret with the buffer solution that you prepared. Make sure that the tips of the burets are filled and that the level of the liquids is at or below the 0.00 mL line. 6. Connect the pH Sensor to LabQuest. Suspend the pH Sensor in the buffer solution, as shown in Figure 1. Make sure that the sensor is not struck by the stirring bar. 7. Transfer 10.00 mL of the buffer solution to a 250 mL beaker and add some distilled water. If a magnetic stirrer is to be used, keep the tip of the electrode above the stir bar. 8. Begin adding the NaOH to the buffer solution in small increments, i.e. 0.05-0.1 mL. After each addition, record the total volume of NaOH added and the pH of the solution in the data table. 9. Continue adding the NaOH solution until the pH has risen at least one pH unit. 10. Repeat as time allows.

Figure 1 Westminster College SIM

Page 4

Preparing Buffers and Buffer Capacity

DATA Buffer Prepared: _____________________________ Concentration: _______________________________ pH of buffer solution __________________________

Trial 1

Trial 2

Trial 3

Initial pH of Buffer mL of Buffer mL of NaOH Final pH

Westminster College SIM

Page 5

Preparing Buffers and Buffer Capacity

CALCULATIONS 1. Summarize the data for your titrations in the table below.

Trial 1

Trial 2

Trial 3

Vol of buffer, mL Vol of buffer, L Change of pH Vol 0.100 M NaOH Moles of NaOH Buffer Capacity

2. Show the calculations for one of the trials above.

3. What was the average buffer capacity for the buffer that your group prepared?

Westminster College SIM

Page 6

Preparing Buffers and Buffer Capacity

QUESTIONS 1. How does the concentration of the buffer affect the buffer capacity?

2. What differences would be observed if HCl were used in place of NaOH?

3. Write equations to show how a buffer works.

Westminster College SIM

Page 7

Loading...

Preparing Buffers and Buffer Capacity - Westminster College

PREPARING BUFFERS AND BUFFER CAPACITY LAB From Juniata College, Science in Motion Westminster College INTRODUCTION A buffer solution is one that is ...

213KB Sizes 0 Downloads 0 Views

Recommend Documents

BUFFERS AND BUFFER CAPACITY
BUFFERS AND BUFFER CAPACITY. By Dr. Jan Pláteník. Principle of buffering. A buffer solution is a solution that resists

Mobile Phase Buffers, Part II — Buffer Selection and Capacity
Aug 22, 2014 - buffering capacity to maintain the target. pH. Buffer capacity or buffer index8 is a quantitative measure

Buffer Preparation and Capacity - Fairmont State College
is a mixture of a weak acid and its conjugate base. The buffering capacity of a solution is determined using the Henders

Buffer Capacity
Thus the buffer is effective as long as it does not run out of one of its components. Page 4. +. ▫ Quantitative measur

Buffer Capacity
In this equation, H = 10-pH, Ka and Kw are the acid equilibrium constant and autoprotolysis of water, respectively, and

Determination of pH and Buffer Capacity
a source for the buffers in one of their experiments. However they do not know which source will best resist a change in

Buffer Capacity, Ecosystem Feedbacks, and Seawater - MDPI
Sep 6, 2013 - As buffer capacity decreases, diel variation in seawater chemistry increases. However, a variety of ecosys

Commodity Fetishism - Westminster College
Oct 9, 2007 - “Commodity Fetishism” in the Era of the Celebrity: A Marxist Critique of the American ... In American

desert solitaire - Westminster College
Eye of the Whale, Upheaval Dome, and. Paul Bunyan's Potty to name a few. The adventures near Moab are numerous and varie

onion rings - Westminster College
cell membrane which allows dissolved materials to enter and leave the cell, and cytoplasm, the fluid that fills each cel