Protein Structure Insights into the Bilayer Interactions of the Saposin ... [PDF]

5 hours ago - Members of the saposin-like protein superfamily have diverse biological functions all involving lipid interactions. Mechanisms by which ... PSIs display bilayer perturbation and membrane permeabilising activities, and their roles in vivo include endosomal sorting and plant pathogen resistance. Biochemical ...
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Idea Transcript

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StAP PSI H3 - EAPLCTACEMAVVWMQNQLKQ Saposin C H1 - YCEVCEFLVKEVTKLID Saposin C H2 - EKEILDAFDKMCSKLPK

EAPLCTACEMAVVWMQNQLKQ.

IVSME_ CKTIVSQY_ GE_ MIWD_ LLVSG (6990 M − 1cm − 1)

H1 :

H2 : H1H2 :

VR_ PD_ QVCSQAGLCF_ V (no absorptivity)

IVSME_ CKTIVSQY_ GE_ MIWD_ LLVSGVR_ PD_ QVCSQAGLCF_ V (6990 M − 1 cm − 1) X:

D_ GAQHVSSNIK_ TVVERE_ TE_ GSSVG (no absorptivity)

H3 :

E_ APLCTACE_ MAVVW_ MQNQLK_ Q (5500 M − 1 cm − 1)

H3 E64Q : QAPLCTACE_ MAVVW_ MQNQLK_ Q (5500 M − 1cm − 1) H3 E72Q : E_ APLCTACQMAVVW_ MQNQLK_ Q (5500 M − 1cm − 1) H3 K83Q : E_ APLCTACE_ MAVVW_ MQNQLQQ (5500 M − 1cm − 1 ) H4 :

ć

E_ GTKEK_ VLE_ Y_ VNQLCEKIP (1490 M − 1cm − 1 )

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