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Vitamin C REPORT (HTTPS://DOCSLIDE.COM.BR/REPORT-COPYRIGHT/VITAMIN-CDocuments (https://docslide.com.br/category/documents.html)
56F290D16D8DA/5681675E550346895DDC2F35) Please download to view (https://docslide.com.br/download/link/vitamin-c-56f290d16d8da) Core Practical Vitamin C is an antioxidant Which type of fruit juice provides the most vitamin C? Is drinking fruit juice as good as eating fruit (for antioxidants)? Fruit juice in cartons is long life (heat treated)
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Quantity of Vitamin C in food and drink determined by colour test Vitamin C decolourises blue dye DCPIP Vitamin C reduces the DCPIP DCPIP changes colour in presence of vitamin C from blue to colourless (or slightly pink) Syringe 1cm3 of DCPIP solution into a test tube Draw some vitamin C solution into the pipette â MAKE SURE YOU KNOW
drop Repeat until the solution goes colourless Take a note of how much you have added YOU SHOULD DO THIS A FEW
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MORE TIMES AND AVERAGE THE RESULTS Record the exact amount you added Repeat the procedure with the other
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HOW MUCH YOU HAVE IN THERE Add the 1% vitamin C sample to the test tube drop by drop Shake gently after each
The 1% solution contains 10mg of vitamin C per 1cm3 What is the mass of vitamin C required to decolourise 1cm3 of the DCPIP? (in mg per cm3) What is the question you are testing? List of apparatus Describe the method What are the independent (type of solution/vitamin C content) and dependent (volume of solution dropped in to decolourise) variables? What other variables might affect the results (temperature, other chemicals present, age of juice)? What is the control (1% vit C solution)? Why do the replicates (for reliability)? Discuss and support your conclusions with relevant biological knowledge and evidence
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from data Comment on any systematic or random errors What changes would you make and why - do more repeats? (reliability), use a colorimeter to measure colour change? (validity) , use a different control e.g. water (validity)? Juice tested Volume of juice required to decolourise 1 cm3 of 0.1% DCPIP solution/cm3 Average of juice required /cm3