XI International Symposium on Experimental Techniques XI

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2011 5 (2)

I LUSO-BRAZILIAN CONGRESS OF THE EXPERIMENTAL PATHOLOGY

XI International Symposium on Experimental Techniques Federal University of Pernambuco

CPE-1360 1

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sponse to Cyclosporin A Ivana Jarak, Ludgero C. Tavares, John G. Jones, Rui A. Carvalho Center for neuroscience and Cell Biology, Coimbra, Portugal Cyclosporin A (CsA) is an immunosuppressive drug frequently used for prevention of allograft rejection and the treatment of autoimmune diseases. Despite the desired effect on the immune system, long-term use of CsA is associated with adverse effects that have adverse impact on the quality of life and the survival of patients. Acute rotoxicity, and renal dysfunction. Although the underlying molecular mechanisms of CsA toxicity are not fully understood, they involve oxidative stress, apoptosis and

rapid and multicomponent analysis of all the important -

of Pernambuco, Brazil 2. Department of Pathology - Federal University of Pernambuco, Brazil development of laceration in the middle layer from intima -

secting hematoma were collected from cadavers were em-

where investigated sex, age and personal history. Results: The mean age was 71.8 years for male when compared with 56.5 years old in the female. Among the diseases are more common in men, the hypertension cases involving

of the male cases, moderate atheromatous lesions were toma was installed in the proximal segment (thoracic) at n

evaluate the hepatic response to CsA treatment and to asment of detrimental side effects of CsA treatment. Thus, 16 non-transplanted Wistar rats were treated for 2 weeks livers freeze-clamped for analysis. The same experiments were performed after one day CsA administration. Meta-

the aortic aneurism dissecans is noted in women with a -

lised liver tissue samples. 1H NMR spectra were acquired at 14.1 T Varian spectrometer equipped with a 3-mm in-

women. Keywords: Dissecans aortic aneurism; Macroscopic; Epidemiology

nent analysis (PCA), using a Spearman correlation matrix

CPE-1374 Nitric oxide is responsible for oxidative skin injury after 24h of UVB exposure V. A. Terra1, F. P. Souza-Neto1, L. N. Z. Ramalho2, R. C. Pereira1, T.N.X. Silva1, R. Cecchini1, A. L. Cecchini1 1. Laboratório de Fisiopatologia e Radicais Livres, Universidade Estadual de Londrina (UEL). Rodovia Celso Garcia Cid, PR445, km 380, Campus Universitário, 86051-990, Londrina, PR, Brasil. 2. Dept. Patologia e Medicina Legal, Universidade de São Paulo (FMRP-USP), Ribeirão Preto, São Paulo, Brasil

XLSTAT 2009 (Addinsoft). We found that although the

visual comparison of normalized spectra, which allows for tentative population distinction under given treatment regime (2 week CsA administration). PCA analysis of lipid extract NMR spectra indicate changes in energy (tendency toward glycogen depletion, increased acetate and alanine levels) and lipid (decreased levels of choline intermediary . CPE-1372 Macroscopic and epidemiological analysis of cases of dissecans aortic aneurysm 1 .; Araújo Duke, M.A 1.; Modesto Araújo,M 2.; Campos Maia, L 2.; Pontes Filho, N. T 1,2.; Melo-Junior, M. R 1,2. 1 - Immunopathology Laboratory Keizo Asami - Federal University

mammalian cells and plays a variety of regulatory roles jectives: This article provides evidence regarding the par-

radiation the skin was used to determine the redox capacity 9

Experimental Pathology and Health Sciences

munohistochemistry was also performed for proliferating cell nuclear antigen (PCNA), nitrotyrosine, and vascular endothelial growth factor (VEGF). Results: Considering

A highly sensitive NO detection system using chemilumi-

140.6) and the increase in the NO levels (41.0 x 106 ± 9.52

of NO in this organs. Keywords: Nitric oxide, chemiluminescence, biological systems

mice were treated with aminoguanidine (AG) lipid peroxi±142.7) and shows decrease in NO levels (19.5 x 106 ± 2.7 x 106), control (15.4 x 106 ± 1.3 x 106). PCNA and

CPE-1376 Epidemiological analysis of tetanus in accidental Brazil during the period 2008 to 2011

indicating an increase in proliferative potential when iNOS lated to lipid and protein oxidative cell injury. Through, it is evident that NO participation is essential to degenerative radiated skin. This work shows that the NO play an essential part in the modulation of proliferative and oxidative

1.Departamento de Enfermagem – UFPE/ Brazil 2.Departamento de Ciências Biológicas – UFPE/ Brazil Background: Tetanus is an acute infectious disease, not

ioral risk than as a disease, thus not presented in epidemic

Keywords Nitric oxide, lipid peroxidation, antioxidants, UVB skin irradiation, proliferation markers, nitrotyrosine tetanus reported in Brazil. Methodology: For this we used CPE-1374a Nitric oxide detection by luminol-H2O2-induced chemiluminescence in biological system and chemical NO- donors systems Terra, VA1; Panis, C1,2; Yamamoto, C1; Souza, FP1, Cecchni, AL1, Cecchini, R1. 1.Laboratory of Physiopathology and Free Radicals, Department of General Pathology, State University of Londrina, Londrina, Paraná, Brazil. 2.Stem Cells Laboratory, National Institute of Cancer, Rio de Janeiro, Brazil. ological tension of oxygen (O2) and its high reactivity

jective: In this study our purpose was to compare and tive of NO with the luminol – hydrogen peroxide (H2O2) -

race, area of residence, education and evolution) that are associated with incidence and mortality of tetanus. Results: During this period 1137 cases were reported, of which

clusion: According to the data, the male is the most afindividuals in adulthood, unlike women that there is a vaccine strategy, aimed at prevention neonatal tetanus, during a need to make an expansion of coverage, especially for those individuals most affected, in addition to having an improvement of access and assistance of health services. Keywords: Accidental tetanus. Epidemiology. Clostridium tetani. CPE-1376a nambuco, from 2009 to 2011

prevented fast NO oxidative degradation through removal analysis was in fact NO. We can conclude that this reaction gate the nature of reactive species enrolled in NO-chemiluminescent reaction, all evaluated NO-donnors systems

1.Departamento de Enfermagem – UFPE/ Brazil 2.Departamento de Ciências Biológicas – UFPE/ Brazil Background: Schistosomiasis is a parasitic infection caused Schistosoma mansoni, to which the symptoms will depend on the evolution of the parasite in the host, and man its main reservoir. The transmission of the human through infected snails. Infection may present as acute and the clinical picture progressively evolves to chronic infection

reaction and on the same concentrations. Results: The re10

2011 5 (2)

tion. In a period of three to seven weeks of exposure, the picture can emerge of acute schistosomiasis, also known

This study aimed to analyze the frequency of individuals It was made the use of Sistema Nacional de Agravos de

area of residence and course of infection, which are associated with incidence and mortality of schistosomiasis. Results: During this period, 1938 cases were reported, of -

for more effective programming strategies in relation to the control of transmission, as well as the performance of of individuals affected, with a focus on higher risk group. Keywords: Schistosomiasis, Public health, Epidemiology CPE-1379 Effect of essential oil of Mentha crispa on young worms of Schistosoma mansoni and histomorphometric analysis of liver tissue in experimental schistosomiasis Oliveira, A.P.1; Franco, E.S.1; Melo, R.G.1; Aires, A.L.2; querque, M.C.P.2; Maia, M.B.S1. 1. Department of Physiology and Pharmacology UFPE, Brazil 2. Laboratory of Immunopathology Keizo Asami - LIKA/ UFPE, Brazil 3. Faculty of Pharmacy, University of Porto, Portugal 4. Department of Pathology UFPE, Brazil 5. Department of Antibiotics UFPE, Brazil Background: The schistosomiasis mansoni is a grave tion is the main mechanism involved in the pathogenesis. Studied show resistance to treatment with praziquantel, in The aim of this study was to evaluate the antischistosomal activity of essential oil of Mentha crispa (EOMc) on S. mansoni infected mice. Methodology: Forty female Swiss mice, weighing around 20-25g, infected with 50 cercariae

tion in the diameter of the granulomas (349.29±7.96, 320.72±10.24 and 355.89±11.38µm) in the groups treated compared to control 393.86±6.81µm. Conclusions: Treatment with EOMc demonstrated schistosomicidal properties on young worms of S. mansoni, improvement of hepatoesplenomegaly and reduction of the diameter of the granulomas. Keywords: Schistosoma mansoni, Mentha crispa, essential oil CPE-1379a Evaluation of the expectorant activity of acid fumarprotocetrárico isolated from the lichen Cladonia verticillaris in swiss mice Alves, G.M.B.1; Franco, E.S.2; Oliveira, A.P.2; Cunha, H. P.2; Melo, R.G.2; Cordeiro, D.P.2; Pereira, E.C.G.3; Silva, N.H.1; Maia, M.B.S.2 1 Department of Biochemistry and Physiology – CCB/UFPE, Brazil 2 Department of Physiology and Pharmacology – CCS/UFPE, Brazil 3 Department of Geographical Sciences – CFCH/UFPE, Brazil cine since ancient times to treat many kinds of respira-

verticillaris in swiss mice. Methodology: We used 30 swiss -

after treatment the animals where anesthetized, tracheostomized and cannulated, and we carried out a cycle of ing a spectrophotometer at 535nm. Results: The results expressed as mean ± standard error of mean. And these

infection the animals were treated oral with 60, 80, 100 ten consecutive days. In the 47th day after infection the mice were euthanized for evaluate of the worm load, oo-

± 0.012 and 0.285 ± 0.003, respectively) than that shown

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expectorant activity in mice. Keywords: acid fumarprotocetraric, expectorant, lichens served in liver weight (7.22±0.28, 7.11±0.53 e 7.54±0.52)

CPE-1380 Analysis of clinical requirements associated with drug interactions of a public hospital in Pernambuco 11

Experimental Pathology and Health Sciences

1.ASCES University, Caruaru-PE 2.Microbiology Laboratory-UFPE-PE Background: The simultaneous use of drugs, common

age, occupation, location of the lesion and assessing the We conducted a descriptive analysis of the data and the

modify the activity and pharmacological response. In patients taking ten to twenty drugs for interactions incidence

the location of the lesion to the inner surface of the lower

includes the ongoing evaluation of the relationship for tion of the hypotheses suggested in the clinical diagnosis which the results outweigh the risks to which patients are

interaction with major related to drugs-patients and the administration. OBJECTIVES: Analyze the clinical requirements of the hospital identifying incidence of drug interactions. METHODOLOGY: A total of sixty prescriptions prescriptions contained ten or more medications that were analyzed individually considering the potential for drug-

at least two interactions occurring up to seventeen interactions in the same prescription. The drugs clonazepam, quent prescriptions in the hospital environment, especially where there is no clinical pharmacy service. These drug incus on therapies involving ten or more drugs which often

tween the most prevalent lesions with lesion location and tention of health services for patients with this oral condition. Keywords: Biopsy, Mouth Sores CPE-1381 Salivary glands in complementary treatment experimental hyperthyroidism Alves da Silva, M.A.1,2, Nery da Silva, E.1,2, Araújo Félix Junior, J.1,2, Campos Oliveira, M.1,3, Souza Freitas,V.1,3, 2 Farinha Henrique Marques, R.H.2 1. Health Department - State University of Feira de Santana, Brazil 2. Experimental Pathology Service, Faculty of Medicine, University of Coimbra, Portugal 3. Oral Cancer Center - State University of Feira de Santana, Brazil -

the effectiveness of treatment and may cause damage to patient health. Keywords: Drug Interactions, Drugs.

of levothyroxine for two weeks. The aim of this study is

CPE-1380a Analysis of the prevalence of oral clinical lesions in two cities of Pernambuco Bianco, L.R.¹, Neves, M.L.R.¹ ,Fiho, L.M.S.¹ 1.ASCES University, Caruaru-PE

in four groups: control group, kept with no manipulation (Ctrl); group II, which received an administration of 250

prevalence of oral lesions demonstrates the great diver-

even the geographic characteristics of each local. Knowing prevalent injuries and demographic characteristics of the population affected is important for prevention and malignant potential reinforces the importance of this ex-

lesions performed in referral centers in two cities of Per12

of acini parotid gland area on experimental hyperthyroid-

group III received levothyroxine and acupuncture treatture treatment. The points used for puncture were: ST09, ST36, HC4, RM23. After two weeks, all the animals were collected and analyzed using ImageJ®. The histopathological analysis shown no signs of glandular lesion. The more cases of smaller parotid gland acini on the acupuncture treated group. The group with experimentally induced hyperthiroidism, as the group with hyperthiroidism plus acupuncture treatment, presented more acini with major areas than the group kept with no manipulation. The results of the present study, although without statistical thiroidism, individually, affects the parotid salivary gland.

2011 5 (2)

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when compared with the control group. Molecular and analysis results. Keywords: Salivary Glands; Morphometrics, Hyperthyroidism CPE-1383 Determination of changes in production of polysaccharide capsule in pathogenic isolates of Klebsiella pneumoniae Gonçalves, G. G. A.1; Veras, D. L.1, 2; Lopes, A. C. S.3; Feitosa, A. P. S.1, 2; Freitas, C. F.1; Vaz, G. S.1; Padilha, R.2; Brayner, F. A.1, 2, 3; Alves, L. C.1, 2. 1. Departamento de Parasitologia do Centro de Pesquisas Aggeu Magalhães CPqAM / FIOCRUZ, Brazil 2. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, Brazil 3.Departamento de Medicina Tropical da Universidade Federal de Pernambuco-UFPE, Brazil and pneumonia. Most of these infections are associated with hospital environments, where it is still one of the

polysaccharide capsule (CPS) consists in one of the most important virulence factors of pathogenics isolates of K. pneumoniae, the aim of this study was to determine the presence of genes for the production of CPS in isolates of K. pneumoniae, as well as to determine the time-kill and ilar concentrations to those levels reached in the human plasma. Methodology: During this study a total of four

and aztreonam, respectively. The Transmission Electron es in the CPS production, after the treatment of two isolates with cefotaxime, ceftazidime and aztreonam in the Results: All isolates presented the gene for CPS production showed morphological changes and electrondense parti-

the symptoms more commons are fever, shivers, nightly sweat, loss of appetite and weight, caugh (over 3 weeks). odology: It was used the Sistema Nacional de Agravos de

race, age group, schooling, form and situation of close. Result: There was predominance of the cases among peoto (51,87) with age group among 20 to 39 years old (45,98)

gave up the treatment. The most incidence of the cases opment proportion to the cure regarding the desertion of the treatment is an indicative that the health actions have gies they refer to the promotion, control and the acting of the epidemiologic vigilance mains to promote the prevention, cure and reduction of the transmission risc. Keywords: tuberculosis, epidemiologic vigilance, epiCPE-1385 Effect of swimming in peripheral nerve regeneration after experimental neurotmesis Moraes, S.R.A.1; 1. Departament of Anatomy, Universidade Federal de Pernambuco, Brazil. 2. Faculty of Medicine, Universidade Federal do Ceará, Brazil. 3. Departamento f Anatomy, ICB III, Universidade de São Paulo, Brazil. Background: The injuries of the peripheral nerves have negative impact on the functionality of the individual, can help in the process of peripheral nerve regeneration. exercise in water on the peripheral nerve regeneration after injury neurotmesis. Methodology: Were used 14 male rats (Wistar), randomly separated in the Control Group

the CPS production. Conclusions: The results showed that

days of life, the GLN group was adapted to the aquatic

in reducing the CPS production. Keywords: Klebsiella pneumoniae; Polysaccharide

neurotmesis sciatic nerve surgery of the right foot, and the with Matrigel® and saline solution. 48 hours after the injury, the GLN group started swimming protocol lasting 30 minutes, 5 times a week, for 28 days, while the GLSN was

CPE-1384a during the period 2008 to 2010 Santos, C.R.C¹ ; França, M.J.D.M¹; Soares, L.H.S¹; Da Silva, P.K.F¹; Avelino, J.E¹; Leal, T.V¹; Lima, V.P1; Júnior,

60 days of injury, all animals had their right sciatic nerve collected for histomorphometric analysis. The statistical

1

1 Universidade Federal de Pernambuco, Brasil

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Experimental Pathology and Health Sciences

injury was lower than in the control group, no having difference when compared GLSN and GLN groups. There

Ethics Committee of the Health Sciences Center of the

GLN, compared to the GC and the GLSN. Conclusion: The swimming protocol adopted optimized the process complete lesion of the sciatic nerve, without compromising the regenerative process. Key-words: Neurotmesis; Nerve Regeneration; Swimming CPE-1387 Advances in the diagnosis of cervical cancer caused by HPV Nunes, M. K. S.1,2; Gomes, A. M. P.1,2; Silva, B. D. F.1; Machado, W. G. S.1; Santos, E. P.1; Souza, J. P1, 2; Santos, A. H.1; Cardoso, H.M1; Pereira, B. D. S.1; Costa, L. A.1; 1. Faculdade Santa Emília de Rodat, FASER, Graduandos em Biomedicina, João Pessoa, PB, Brasil. 2. Universidade Federal da Paraíba, João Pessoa, PB, Brasil. lem. However, improvements in diagnosis and prognosis discovery that human papillomavirus (HPV) infection is a crucial part of the causal pathway of the pathogenesis screening processes and has asking for more investigawith the screening improvement, advances were validated to increase the detection of early stages of neoplastic diseases. Early detection allows not only the surgical treat-

advances were achieved in three main areas: protein prowith mass spectrometry techniques and cutting-edge highperformance validation. These new technologies represent markers in order to incorporate in the future these applications in molecular diagnosis of cervical cancer. Keywords: Advances, diagnosis, cervical cancer

clusion: The main heart diseases were: atrial septal defect cords. It is recommended to health professionals to keep Keywords: Neonatal Nursing, Congenital Heart Disease, Newborn CPE-1391 Investigation of cytotoxicity of plant extracts in northeastern Brazil Vilar, G.N.1; Melo, A.F.C.1; Trindade, A.S.P.1, Brito, I.M.S.C.1; Costa, V.C.O.1; Silva, M.S.1; Tavares, J.F.1; Silva, T.M.S.2; Cavalcanti, F.A.3; Pessôa, H.L.F.1 1.Departamento de Biologia Molecular, Universidade Federal da Paraíba, Brazil 2.Departamento de Química, Universidade Federal Rural de Pernambuco, Brazil 3.Instituto de Ciências Biológicas e da Saúde, Universidade Federal da Alagoas, Brazil Solanum paniculatum L. and Croton grewioides Baill. are used in folk medicine for treatment of gastrointestinal diseases. The evaluation of potencial toxicity of natural prod-

a simple model o study protective and toxic effects of a vestigate the cytotoxicity of ethanolic extracts of the stem oides through hemolytic and hemagglutinating evaluation. Methodology: Blood sample (A, B and O) was mixed with lution of NaCl. The extracts were added to suspension of erythrocytes at various concentrations (1, 10, 100 and 1000 perature under 100 rpm. After this time they were centrifuged at 2500 rpm for 5 minutes and hemolysis was mea-

CPE-1390 Heart Diseases in the neonatal period in a hospital school of Recife - PE Silva, V. B.¹; Ferraz, I. C.¹; Silveira, M. M. B. M.¹; Siqueira, D. C. C.1; Silva, D. M.1; 1. Dep. de Enfermagem - Universidade Federal de Pernambuco (CAV) – Brasil the time the child reaches the age of 27 days, 23 hours

to induce or prevent de clot formation of erythrocytes of

investigate the congenital heart diseases in the neonatal period in a hospital school in the city of Recife, in the period January to June 2011. Methodology: A descriptive study was conducted exploratory in nature cross through

cytotoxicity e no effect on hemagglutination. Keywords: Plant extracts, hemolysis, hemagglutination

period January to June 2011. For collecting data was drawn 14

CPE-1393 Seroprevalence of HIV and syphilis among inmates from Caruaru, Pernambuco, Brazil -

2011 5 (2)

cena, W.A.T.1; Lima, P.C.S.1; Silva, L.S.F.2; Guedes, R.B.2, Brito Tiago, G.G.3 1 Faculdade Associação Caruaruense de Ensino Superior (ASCES), Caruaru, Brazil 2 Setor de Enfermagem do Presídio Juiz Plácido de Souza, Caruaru, Brazil 3 Laboratório do Hospital Casa de Saúde Bom Jesus, Caruaru, Brazil Background: The prison population is a high risk group for HIV and Syphilis, so screening test and counseling

and to identify risk factors for these infections among We conducted a cross-section in the period from May to July 2011, where 1,097 inmates at a prison in Caruaru were evaluated. We performed a structured interview

respectively, have already come into contact with these protective equipment. However two participants reported not using all this equipment. Conclusion: Although students have not found seropositive for HCV, many assessed through needlestick accidents with sharp material thus meriting a further analysis in this population. Keywords: Hepatitis C, dentist, prevalence CPE-1394 Screening for antimicrobial activity among bacteria isolated from amphibians (Anura) Duarte, S.S.; Monteiro, S.C.G.; Gadelha, C.A.A.; SantiGadelha, T.; Pessôa, H.L.F. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Brazil Background: A powerful approach to discover novel me-

(MHA-TP). Results: The prevalence of HIV infection was

10.1 years, with a minimum age of 18 and maximum of 94

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The association with HIV infection was statistically sig-

transmitted infection. Conclusion: The prison population is a group at high risk for these diseases and screening tests

ics. Methodology: Bacteria were grown in Mueller Hinton ture media were centrifuged at 6000 rpm for 15 min and the supernatants were frozen for 24h. The resulting lysates 7 isolates was carried out against 7 indicator strains: Bacilerichia coli ATCC 10536, Pseudomonas aeruginosa ATCC 9027, Pseudomonas aeruginosa ATCC 25619, Staphylococcus aureus ATCC 6538, Staphylococcus aureus ATCC growth was evaluated. The resistance of the isolates to amoxicillin, ampicillin, gentamicin, streptomycin and tet-

need to implement prevention programs so that they will not spread within this population. Keywords: HIV, Syphilis, Prison CPE-1393a Seroprevalence and risk factors for hepatitis C virus in students of dentistry in Caruaru-Pe K.B.1, Menezes, A.F.1 1.Faculdade Associação Caruaruense de Ensino Superior – ASCES Background: The working conditions of dentists do with

All the isolates were active against E. coli ATCC 2536, P. aeruginosa ATCC 25619 and S. aureus ATCC 25925. One

and P. aeruginosa ATCC 9027. Two isolates were resistant zones higher than 11 mm. All isolates were resistant to ampicillin and only two were resistant to gentamicin and streptomycin. Conclusion: The results indicate that the potential of these microorganisms to produce antimicrodrugs. Keywords: Amphibians, antimicrobial, bacteria

risk factors for HCV in students of dentistry in CaruaruPE. Methodology: Was conducted a cross-section in the in the last two periods of the dentistry course were evaluated. Was performed a structured interview and some data

were no students tested positive for anti-HCV. Character-

CPE-1399a Acute toxicity of essential oil from leaves of Rollinia leptopetala RE Fries in mice Moreira, M.M.B.1; Brito, M.T. 1; Mangueira, V.M. 1; Batista, T.M1; Viana, W.P.1; Pessoa, D.R1; Sousa, T.K.G.1; Tavares, J.F.1; Diniz, M.F.F.M.1; Castello Branco, M.V.S.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brazil respecting their toxicological risks. Rollinia leptopetala RE

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Experimental Pathology and Health Sciences

Keywords: Preterm, socioeconomic, maturity ate the safety of essential oil from leaves of Rollinia leptopetala (E.O.R) determining its potential toxicity. Methodology: For the acute study, the E.O.R. was administered determined for up to 14 days. Indexes of organs (liver, heart, kidneys, spleen and thymus) were evaluated to de-

effects, such as hypnosis, ptosis, sedation, constipation, after the i.p. administration and were more pronounced -

CPE-1401 Evaluation of antimicrobial activity and genotoxicity of plant extracts in northeastern Brazil Melo, A.F.C.1; Vilar, G.N.1; Brito, I.M.S.C.1; Saraiva, M.G.1, Costa, V.C.O.1; Silva, M.S.1; Tavares, J.F.1; Silva, T.M.S.2; Cavalcanti, F.A.3; Pessôa, H.L.F.1 1. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Brazil 2. Departamento de Química, Universidade Federal Rural de Pernanbuco, Brazil 3. Instituto de Ciências Biológicas e da Saúde, Universidade Federal da Alagoas, Brazil Introduction: Plants supply most of the active ingredients well documented that gene mutations act in the process of carcinogenesis and assays that detect genotoxic comTul., Solanum paniculatum L. and Croton grewioides Baill.

perimental animals. Conclusions: E.O.R. showed moderate acute toxicity when administered i.p. to mice. RepeatedKeywords: Rollinia leptopetala; acute toxicity; essential oil CPE-1400 Preterm birth and the possible implications of the socioeconomic background and the maternal maturity Pereira, A.F.¹; Alves, I.S.¹, Menezes, R.C.L.¹; Anjos, F.B.R¹,

tory Concentration (MIC). It was used Gram-negative and erichia coli ATCC 2536 and ATCC 10536, Pseudomonas aeruginosa ATCC 8027 and ATCC 25619, Staphylococcus (630 nm). The genotoxicity of three species extracts (1, 10, 100 and 1000 ug) was evaluated in Salmonella thiphymurium strains TA 97, TA 98, TA 100 and TA 102 through

1-Universidade Federal de Pernambuco – Brazil 2-Maternidade Professor Bandeira Filho – Brazil and socioeconomic level of the pregnant women attended in the Professor Bandeira Filho maternity (PBFm). Methodology: This research was transversal, trough semi-direct questionnaires applied in PBFm, in the years of 2006 and 2007. The analyzed data were: family income, education

strains tested and showed no genotoxic effect. Conclusion: toxic potencial. Keywords: Plant extracts, antimicrobial activity, genotoxicity CPE-1386 Research of helminths larvae in the main beaches from João Pessoa, Brazil De Sousa, T.A.1; Almeida, R.N.1; Leite, G.G.F1; Gomes, A.M.P.1 ; Santos, E.P.1; Cardoso, H.M.1;Santos, S.G.2 1. Santa Emília de Rodat College, FASER, Students of Biomedicine, João Pessoa, PB, Brazil. 2. Santa Emília de Rodat, College,FAZER. Sócrates Golzio Biomedicine professor of the clinical biochemistry and parasitology subject. João Pessoa,PB, Brazil. main cause of contamination the faeces of domestic ani-

disciplinary team may offer an alternative to correct these elements also seen in this population. 16

cara sp. and the Cutaneous migrans larvae, which are the main etiologic agents Ancylostoma sp. and the Strongiloi-

2011 5 (2)

performed mainly in the aspect to diagnose precisely and consequently to treat the association of pathologies. Keywords: Advances, diagnosis, Kaposi sarcoma so that four have formed a square with 10 m each side, and one in the center of the square, the depth in which

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CPE-1389 Liver and adipose tissue in experimental DMBA administration Patricia Jesus1, Pedro Sá2, Nuno P. F. Gonçalves1 2 Canelas1, Mariette M. Pereira1 1. Chemistry Department, University of Coimbra 2. Institute of Experimental Pathology, Faculty of Medicine - University of Coimbra -

de Gramame; Praia do Sol, and Seixas. From the larvae cluded that it is necessary preventive measures to avoid

some tissues and develop a carcinogenic action. The goal of this study is to quantify the presence of DMBA in liver and adipose tissue in the experimental short-term intoxica-

sand contamination. Keywords: Helminths; larvae; beaches CPE-1386a Kaposi sarcoma (SK) associated to the acquired im-

weeks; and the control group, kept on the standard conditions. At the end of the assay time, all animals were ethically killed and the complete necropsy performed. Frag-

Cardoso, H.M.1; Sousa, T.A.1; Santos, A.H.1; Silva, S.R.1; Gomes, A.M.P.1,2; Nunes, M.K.S 1,2; Silva, B.D.F.1;

for routine histopathological processing and examination, and for snap freezing in liquid nitrogen and preservation at -80ºC. A liquid-liquid extraction protocol was used to isolate the lipophilic portion of the liver and adipose tissue

de Carvalho, M.M.S.3. 1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil 2. Federal University of Paraiba, student of Technical Biodiagnostic, Brazil. 3. Biological Science Center, State University of Paraiba, Brazil Introduction: The SK is a proliferative neoplasm associated to immunosuppression conditions that affects the

The Lee index was calculated and showed only one animal with values under 0,30. This animal had the higher scores sue) and the higher scores concerning to edema on the

human herpes virus type 8 (HHV8). In patients with the cy in male individuals. In a clinical point of view, the SK identify how occurs the association of SK with AIDS, in of known literature, as well as a critical and comparative Results and Discussion: In the systematic research from innovations in the diagnosis, as one of the studies that suggests the roles of the cocktail ABCB1 and other drugs in phasized the aspect treatment where one of the studies has evaluated the gene segmentation associated to varied doses of gaciclovi and zidovudina getting results that the target

animals presented adipose tissue haemorrhage and conlesions, particularly of the liver, and the measured quantity of DMBA. The DMBA concentrations found on liver are related with the doses found on adipose tissue, with greater concentrations on the last one. The results of this the adipose tissue, with a correlation with the concentration on the liver. CPE-1395 Evaluation of awareness: Breast cancer and cervical cancer with teachers from private schools in the city of Patos-PB Fernanda D.¹, Pereira, Joana D. C.¹, Dutra, Maria M. G.¹,

AIDS mainly in the regions from the African continent

1. Graduating in Biomedicine- Faculdades Integradas de Patos – FIP. 2. Lecturer in Biomedicine- Faculdades Integradas de Patos – FIP.

present great incidence of the SK related to HIV present

cervix of the teachers of private schools in the city of 17

Experimental Pathology and Health Sciences

Patos. Methodology: We conducted a questionnaire with -

cited. Conclusions: The research pointed to a good level uting to ongoing guidance and conscious of the importance of prevention and early detection through dialogue with society. Keywords: Breast cancer, cervical cancer, Teachers, Private Schools CPE-1396 A quick method for diagnosis of mycotic infections of the eye by using lactophenol cotton blues stain: A case report Marsden, A1; Leite, E1; Magalhães, O.M1; Souza Motta, Massa, D; Gaioso, F2; Neto, G2. 1. Laboratório de Micologia Médica, Centro de Ciências BiológicasUniversidade Federal de Pernambuco. 2. Hospital das Clínicas, Universidade Federal de Pernambuco. illness where fungi are involved. There are; Mycotic Keratithat occurs due to a dissemination of the systemic infectorial examination for fungus infections. Methodology: After scrapping the cornea the material was prepared for direct smear. In the preparation technique, instead of using potassium hydroxide mount, we tried lactophenol cotton many fungal structures. The most of hiphae and mycelium and other fungi cells presented so typical and very well stained. Conclusion: According to our method, the use of result than potassium hydroxide in this case. In this case we isolate Fusarium equiseti and the patient was treated cure after two months of treatment. Keywords: Mycotic Keratitis. Fusariosis, Mycotic ulcers CPE-1402 Intestinal villi changes in experimental administration of semicarbazide 1 3 , Pedro Marques2 , Fernan1 do Capela e Silva3, Rodrigo Farinha1 1. Experimental Pathology Service, Faculty of Medicine, University of Coimbra 2. Faculty of Pharmacy, University of Coimbra 3. Mediterranean agronomical and environmental sciences Institute, University of Évora conducted this study to evaluate morphologic changes of the small intestine related to this disease. Thirty animals 18

the complete necropsy was performed. The small intestine was collected for histopathological processing and analysis. Pictures of routine stained slides were taken and the small intestinal villi length measured, using the software ImageJ. - VB) using the average and standard deviation, for the control group. The test groups were compared with the control one. In the control group the percentage of each class was: 2 VS; 16 S; 64 M; 16 B; 2 VB. In the group II the percentage of each class was: 87 VS; 13 S; 6 M; 1 B; 0 VB. In the group III the percentage of each class was: 93 VS; 30 S; 4 M; 0 B; 0 VB. collagen synthesis is necessary to maintain the intestinal villi length. CPE-1402a Intestinal connective compartment in experimental administration of a carcinogen 1 , Karen Cavalcanti2, Pedro Marques3, Rodri1 go Farinha1, Ivone Souza2 1. Experimental Pathology Service, Faculty of Medicine, University of Coimbra 2. University of Pernambuco - Recife 3. Faculty of Pharmacy, University of Coimbra cide mainly used on rice and cotton cultures as insecticide. Although its short persistence in the environment the pes-

and respiratory tracts. This study was conducted to evaluate morphologic changes of the small intestine related to in two groups, with 10 animals each one. The group I was a control group kept with no manipulation for 28 weeks. mals were maintained with no manipulation until the end in all animals. Pictures of routine stained slides were taken and the intestine connective compartment was measured, of intestinal wall thickness in the test group. Considering condition there is a fragility of the intestinal wall. CPE-1403 Histomorphometric study of effect of different doses of olanzapine on bone tissue of mice Brito, V.C.1; Melo, R.C.B.2; Amorim, M.J.A.A.L1; Oliveira, B.D.R.3 1. Animal Morphology and Physiology Department of Federal Rural University of Pernambuco, Brazil

2011 5 (2)

2. Department of Veterinary Medicine of Federal Rural University of Pernambuco, Brazil 3. Caruaruense Association of Higher Education, Brazil Background: Atypical antipsychotics (eg. Olanzapine) sult, serotonin receptor antagonism promotes reduction of hiperprolactinemia, the main factor associated with

adult mice, males were randomly divided into three groups according to the experimental dose used: Control Group

etine groups is also smaller than the saline groups with

on the development of skeletal muscle tissue. Keywords: Fluoxetine; Skeletal Muscle, Muscle Fiber CPE-1404 Alpha-Pinene: Antioxidant And Hemolytic Effects

was performed within 30 days with intraperitoneal application of the drug. At the end of the treatment, mice were weighed. Then the animals were anesthetized via intramuscular and performed the dissection of the lower

H. L. F.3; Diniz, M. F. F. M.1; Souza, M. F. V.1; 1Departamento de Ciências Farmacêuticas, CCS, UFPB/ Brazil. 2Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, CCS, UFPB/ Brazil. 3Departamento de Biologia Molecular, CCEN, UFPB/ Brazil.

the thickness of the proximal and distal epiphysis and diaphysis thickness were measured with the aid of a digital sent for histological processing. Results: Group 2 had a re-

fungi species, an example of them is the alpha-pinene potential oxidant, antioxidant and hemolytic using human erythrocytes as an experimental model. Methodology:

the control group. The length and thickness of the proximal and distal epiphyses showed no statistical differences. when comparing experimental groups to each other, group

animals. Keywords: Olanzapine, an atypical antipsychotic, bone tissue

was investigated in vitro using human erythrocytes under els were measured spectrophotometrically. And the hemolytic assay was performed with human erythrocytes type positive control was erythrocyte suspension and 100 µL

CPE-1403a phometric analysis of the skeletal muscle 2 ; Oliveira, B.D.R. 3; Moraes, Brito, V.C.1 4 S.R.A. 1- Animal Morphology and Physiology Department of Federal Rural University of Pernambuco, Brazil 2 – Physiotherapy Department of Federal University of Pernambuco, Brazil 3 - Caruaruense Association of Higher Education, Brazil 4 – Anatomy Department of Federal University of Pernambuco, Brazil Background: Pharmacological manipulation using drugs which interfere with the serotonergic system, including seAnalyzed the effects of neonatal serotonergic manipulaclei and the cross section area. Materials and methods: 24 male rats, Wistar, of 30 and 90 days old were used. They weight). The animals were divided into Saline Group (GS30 and GS-90) and Fluoxetine Group (GF-30 and GF-90).

the Ethical Committee for Research with Human Specieffect on any concentrations. When human erythrocytes -

have antioxidant potential. The monoterpene induced a exposed that alpha-pinene did not show oxidant or antioxidant action. Also, it was demonstrated low hemolytic activity. However more studies are needed to investigate the toxicity of this monoterpene. Keywords: Monoterpene; Alpha-pinene; Cytotoxicity CPE-1404a Beta-Pinene: Antioxidant And Hemolytic Effects H. L. F.3; Diniz, M. F. F. M.1; Souza, M. F. V.1; 1. Departamento de Ciências Farmacêuticas, CCS, UFPB/ Brazil. 2Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, CCS, UFPB/ Brazil. 3Departamento de Biologia Molecular, CCEN, UFPB/ Brazil. 19

Experimental Pathology and Health Sciences

(81.4±5.5, 53.4±11.5, 27.9±10.7, respectively) vs contential oxidant, antioxidant and hemolytic. Methodology:

phase. In the second phase also decreased the licking time in a dose dependent 50 (54.5±12.0), 100 (17.6±17.6) and 200 (0.0±0.0) seconds vs. control (141.5±34.4) and is similar to morphine (33.7±13.4). Conclusion: Therefore, it is

in vitro using human erythrocytes under oxidative stress -

-

And the hemolytic assay was performed with human eryth-

nisms involved. Keywords: Farnesol, antinociceptive activity, CNS

sis) and the positive control was erythrocyte suspension

CPE-1406 Is Uncaria tomentosa (cat’s claw) a safe natural product to population?

the Ethical Committee for Research with Human Specieffect on any concentrations. When human erythrocytes -

I. M.2. 1. Laboratório de Farmacologia e Toxicologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, FMVZ - USP, SP. 2. Instituto de Ciências Ambientais, Químicas e Farmacêuticas da Universidade Federal de São Paulo - UNIFESP - Campus Diadema, SP. is a vine that grows in the rainforests of tropical countries

have antioxidant potential. The monoterpene induced a immune stimulatory effect. Despite of many studies re-

adage: “if it is natural, it is not danger”, few studies were

however, hemolytic activity demonstrated in high concentrations. Keywords: Monoterpene; beta-pinene; Cytotoxicity

-

CPE-1405 Evaluation Of Antinociceptive Activity Of Farnesol: A Chemical Component Of Some Essential Oils Pereira, W.B.1, Benedito, R.B.1, Junior, W.M.D.O.1, Torres, P.A.1, Morais, L.C.S.L.D.1, Sousa, D.P.D.2, Diniz, M.F.F.M.1, Almeida, R.N.1 1. Laboratório de Tecnologia Farmacêutica - UFPB, Brasil 2. Departamento de Fisiologia – UFS, Brasil Background: Essential oils represent a promising source of cal activities, such as anxiolytic, anticonvulsant and antinociceptive. The farnesol, an isoprenoid alcohol, is present in study investigated the antinociceptive activity of farnesol (FAR) in animal models. Methodology: Male Swiss mice

for 28 days. Every other day food consumption (FC) and

weights, cellularity and histology). Results: Despite of no statistical differences in FC among the groups, it was veri-

Conclusion: At the present moment, this preliminary study rameters evaluated. However, it is important to highlight -

after treatment with farnesol, the animals were treated with

injected under the right paw surface, 30 min after the drugs administration. And it was recorded the lick time (s) of the terphase), the lick time was evaluated for 15 min (second phase). Results: Animals treated with three doses of FAR (0.0±0.0) (0.0±0,0), respectively, vs control (18.1±4.6). It showed very similar results with morphine (0.0 ± 0.0). Animals treated with FAR showed reduced paw licking time 20

evaluate the safety of this plant to population. Keywords: Uncaria tomentosa, toxicity, immunotoxic CPE-1407 Risk factors associated with the health of pregnant women attended in a maternity hospital in Recife Lima, B.L.F. 1; Ferreira S.R.R. 1; Sousa, G.M.G 1; Anjos, F.B.R. 1 1. Federal University of Pernambuco, Brazil. pregnancy. Among the factors that interfere with the normal evolution of the pregnancy include: age, parity, envi-

2011 5 (2)

volved in maternal health. Methodology: The methodology was a cross-sectional with semi-direct questionnaires applied in the Maternity Professor Bandeira Filho, during the years of 2006 to 2007. The following data was collected: family income, education, food consumed and men-

-

the interview, signed a consent form, aware of its contents. Results: The level of education of the pregnant women did not informed. The monthly income of interviewees -

with other groups. Conclusion: These results suggest that DEP and the treatment with Methanol that represent the less drastic extraction of organic compounds and metals in Golgi. Keywords: Explant, Trachea, Pollution

CPE-1408a als and fats in general. Conclusions: All these data indicate aiming at prevention and health promotion. To this end, order to raise awareness of pregnant women and so reduce some risk factors associated with the health of this group. Keywords: risk, health, pregnant CPE-1408 Toxicity of fractions of particulate matter from diesel exhaust on tracheal explants Choqueta de Toledo3, Milton de Arruda Martins3, Adriano Alencar4, Thaís Mauad1, Luiz Fernando Ferraz Silva¹, Paulo Hilário Nascimento Saldiva1 and Mariangela Macchione1 1Laboratório de Poluição Atmosférica Experimental do Departamento de Patologia da Faculdade de Medicina da Universidade de São Paulo. [email protected] 2Laboratório de Terapeutica Experimental da Clínica Médica da Faculdade de Medicina da Universidade de São Paulo. 3Biotério Central da Faculdade de Medicina da Universidade de São Paulo. 4Laboratório de Micro-reologia e Fisiologia Molecular do Instituto de Física da Universidade de São Paulo. Background: Diesel exhaust particles (DEP) have wide

niloticus from Guarapiranga Reservoir, São PauloBrazil Michelly R. R. Silva4, Eduardo O. Villares4, Dolores H. R.F. Rivero4; Maria J. T. Ranzani-Paiva5; Mariângela Macchione¹ 1- Faculdade de Medicina – Universidade de São Paulo - São Paulo – SP. 2- Universidade Estadual Paulista – Campus Litoral Paulista – São Vicente, SP. 3- LABOMAR - Universidade Federal do Ceará – Fortaleza, Ceará 4- Universidade Paulista – UNIP – Campus Paraíso – São Paulo, SP. 5- Instituto de Pesca – São Paulo, SP. programs as a tool to estimate the degradation of aquatic indicator of physiological effects of water contamination lected with heparinized syringes. The frequency of micronuclei and percentage of differential leukocytes was wald-Giens. Liver were removed for elemental analysis in energy dispersive X-ray spectrometer (EDX). The Cu, Zn,

evaluate the toxicity in tracheal explants of DEP: a) pure als), c) Hexane (remove organic compounds of low polarity) and d) Methanol (remove organic compounds of

-

moved and kept in plates (DMEM-F12 and supplements) elements, Fe and Se, were lower in liver of GR. Concluelements in environmental toxicology, such as Hg, Cd and sections and stained: periodic acid Schiff (PAS) to stain neutral mucus in red and Alcian Blue (AB) to stain acidic

essential elements such as Fe and Se. Fe is a constituent of several enzymes related to oxidative processes and cell

were evaluated: acid mucus (AB+), neutral mucus (PAS+), activity of glutathione peroxidase, an enzyme constituent of the antioxidant defense system found in the liver. Ac21

Experimental Pathology and Health Sciences

tration of elements were sensitive to diagnose differences

and Methods: Larvae of A. aegypti were exposed to the

environmental quality. Keywords: Pollution, Biomarkers, Bioacumulation

ried out in duplicate for each concentration. Larvae were

CPE-1409 Protective effect of Indican from leaves of Indigofera suffruticosa on liver of mice bearing sarcoma 180 Nascimento, D.K.D.1; Lima, I.R.1; Oliveira, Y.D.F1; Silva, I.B.1;Vieira, J.R.C.1; Leite, S.P.1 1. Departamento de Histologia e Embriologia- CCB/UFPE, Brazil Background: Indigofera suffruticosa is a plant of the

velopmental stages of larvae were determined at the start of the experiment (0 h) and 24, 48, and 72 h thereafter, and

dican isolated from I. suffruticosa showed that this com-

The aqueous extract of I. suffruticosa in concentration of

are important tools to detect changes histophysiological histologically and morphometrically hepatic sinusoid capfrom leaves of I. suffruticosa. Materials and Methods:

a stereomicroscope (1.2 x). Morphologic Study: Control graphed using a digital video camera (Leica) connected to

A. aegypti that remained in L2 instar and showed disruption on the peritrophic envelope (PE) structure, consequently resulting in a discontinued underlying epithelium, increased gut lumen, and segments with hypertrophic aspects in comparison with control larvae. Conclusion: The developmental delay is directly dependent of morphological changes that occur when the larvae are growing in con-

days old, weighing from 30 to 40g were divided in three 180 and G2 without sarcoma 180. G1 and G2 - Controls with saline; G3 - treated with Indican. It was injected into the right axillary 0.3 mL (around 3x106 cells) the ascitic form of Sarcoma 180 in groups G1 and G3. After 48 hours of tumor implantation, the treatment was initiated of the histological preparations and the photomicrographs were realized using the optical microscope with camera attached. Morphometric evaluation of the diameter of hepatic sinusoid capillaries was performed using the software sults: In G3 group the diameter of the hepatic sinusoid compound Indican from leaves of I. suffruticosa showed a low order of toxicity on hepatic sinusoid capillaries of tive effect. Keywords: Indigofera suffruticosa; sarcoma 180; hepatic sinusoid capilaries CPE-1409a Morphological observation of Aedes aegypti larvae treated with extract of Indigofera suffruticosa Nascimento, D.K.D.1; Lima, I.R.1; Oliveira, Y.D.F.1; Silva, I.B.1; Vieira, J.R.C.1; Leite, S.P.1 1. Departamento de Histologia e Embriologia- CCB/UFPE, Brazil Background: The mosquito Aedes aegypti is a vector that promotes the dissemination of four serotypes of den-

Keywords: Indigofera suffruticosa; Aedes aegypti; larvae CPE-1411 Nephrogenic Systemic Fibrosis Silva, S.R.1; Pereira, B.D.S.1; Santos, A.H.1; Nunes, M. K. S 1,2; Silva, B.D.F.1; Cardoso, H.M1; Machado, W.G.S.1; Costa, L.A .1; Carvalho,V.A1,3. 1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil 2. Federal University of Paraiba, student of Nursing, Brazil. 3. Santa Emília de Rodat, College. Valderi Alves de Carvalho Biomedicine professor . João Pessoa, PB, Brazil. and rare disease which mainly affects the skin of renal

muscle contractures around joints. The distal areas are face is rarely never compromised. It may affect the skeletal muscles and internal organs such as diaphragm, lungs, and on a systematic review of the medical literature. Method-

with FSN. However, patients affected with the disease have mation, including increased levels of C -reactive protein, serum ferritin, erythrocyte sedimentation rate, and a reused in MRI that seems to only affect patients with advanced renal dysfunction. Some studies have shown an in-

-

-

morphological changes on larvae of A. aegypti treated with aqueous extract of leaves of I. suffruticosa. Materials

tive treatment or prevention. However, recent studies have

22

2011 5 (2)

shown strong association with gadolinium. Experts rec-

and NSF, for security reasons. kidney failure CPE-1411a The treatment of the chronic Chagas cardiopathy through stem cells transplant from the bone marrow Silva, S.R.1; Pereira, B.D.S.1; Santos, A.H.1; Nunes, M. K. S 1,2; Santos, E.P.1; Silva, B.D.F.1; Cardoso, H.M1; Machado, W.G.S.1; Costa, L.A .1; Carvalho,V.A1,3. 1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil 2. Federal University of Paraiba, student of Nursing, Brazil. 3. Santa Emília de Rodat, College. Valderi Alves de Carvalho Biomedicine professor . João Pessoa, PB, Brazil. opathy which affects millions of people in Latin America. Studies with experimental models of IC due the DC indicate that the transplant of mononuclear cells from the tential of these cells in specialized types, like cardiomyomyocardial in patients with heart failure from Chagas etiology and its response as therapy, in a literature review in an exploratory and analytical way. Methodology: The pres-

these ones, three have involved the TCMO in patients with chronic Chagas cardiopathy and one has involved the same transplant in animals with Chagas cardiopathy. There were not any complications directly related to the proce-

Gadelha, C. A. A.1; Santi-Gadelha, T. 1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil 2. Student of the Graduate Program in Nutrition Science – UFPB, Brazil 3. Student of the Graduate Program in Biochemistry – UFC, Brazil 4. Student of the Graduate Program Bioactive Natural and Synthetic Products – UFPB, Brazil Introduction: Lectins are a group of (glyco)proteins that

of this study is to assess the oxidant and antioxidant acWe used Triton X-100 to promote hemolysis and exposure a spectrophotometer at a wavelength of 630nm and 540 serving whether AEL protects the erythrocytes against the done with a Pasteur pipette, due to the need for oxygen

Phenylhydrazines. In erythrocytes treated with AEL, the AEL showing that acts as antioxidant compound decreas-

-

lectin showed little relation to oxidant Phenylhydrazines tion of this agent. Keywords: Lectin; Abelmoschus esculentus; Oxidation

markers or neuro-hormonal. Other study involving ani-

CPE-1412a Isolation and Characterization of a Lectin from Seeds of Mucuna pruriens

cate that the therapy with stem cells has a promising future, the initial results of the response to the therapy with

1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil 2. Student of the Graduate Program in Biochemistry – UFC, Brazil 3. Student of the Graduate Program in Nutrition Science – UFPB, Brazil

peutic effectiveness of the mentioned procedure. Keywords: Chagas cardiopathy, stem cells transplant, cell therapy CPE-1412 Abelmoschus esculentus lectin reduces oxidant action of phenylhydrazine Gomes, V. M.1; E-Lacerda, R. R.1; Caluete, M. E. E.2; Leite, J. F. M. 3; Pessoa, H. L. F.1; Gonçalves, G. F.4;

-

a lectin from the seeds of Mucuna pruriens. Methodolwhich were tested for the presence of hemagglutinating 23

Experimental Pathology and Health Sciences

chromatographed on a column of molecular exclusion Sephadex G-100. All proteins were eluted protein disity and resistance to changes in pH was determined using

Keywords: Sterculia foetida; Pharmacology; Protein CPE-1412c seeds of Clitoria fairchildiana

resistance to proteolytic enzymes was determined using chelating agent was determined using EDTA. Beta-mercaptoethanol and urea were used to determine the effect of reducing agents, as well as the metaperiodato sodium was used to verify the action of oxidizing agent. In all experiments, the hemagglutinating activity was determined

1. Laboratory of Biologically Actives Molecules - Federal University of Ceará, Brazil 2. Laboratory of Biochemistry, Genetics and Radiobiology - Federal University of Paraíba, Brazil University of Ceará, Brazil

chromatography. Its molecular mass was estimated at 116

-

change its structure, as well as any reducing agent. The from seeds of Clitoria fairchildiana. Methodology: Paw hemagglutinating activity. Conclusion: It was isolated and partially characterized a lectin from the seeds of Mucuna pruriens approximately 116 kDa. More studies are under-

-

Keywords: Lectin; Mucuna pruriens; characterization CPE-1412b Prospecting Biochemistry and Pharmacology of Protein Fractions of Sterculia foetida

were calculated using the trapezoid method and expressed -

1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil 2. Student of the Graduate Program in Biochemistry – UFC, Brazil 3. Student of the Graduate Program in Nutrition Science – UFPB, Brazil Background: Currently the plants are still an unknown and -

spectrophotometry (A600nm). Peritonitis model: Lectin

and differential leukocyte counts. Results: The lectin-like Conclusion: The lectin-like from C. fairchildiana presents

Sterculia foetida. Methodology: The seed meal has prochildiana of type A, B and O, using the chemical Hemolyzing Trius, causing you to read the results in a spectrophotometer glutelin showed hemagglutinating activity. All fractions

aeruginosa, Escherichia coli and Staphylococcus aureu. By SDS-PAGE showed the variety of protein fractions

CPE-1412 Abelmoschus esculentus lectin reduces oxidant action of phenylhydrazine Gomes, V. M.1; E-Lacerda, R. R.1; Caluete, M. E. E.2; Leite, J. F. M. 3; Pessoa, H. L. F.1; Gonçalves, G. F.4; Gadelha, C. A. A.1; Santi-Gadelha, T. 1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil 2. Program in Nutrition Science – UFPB, Brazil 3. Program in Biochemistry – UFC, Brazil 4. Program Bioactive Natural and Synthetic Products – UFPB, Brazil Introduction: Lectins are a group of (glyco)proteins that

in seeds of Sterculia foetida. All protein fractions of the logical use of its constituents. 24

of this study is to assess the oxidant and antioxidant ac-

2011 5 (2)

We used Triton X-100 to promote hemolysis and exposure a spectrophotometer at a wavelength of 630nm and 540 serving whether AEL protects the erythrocytes against the done with a Pasteur pipette, due to the need for oxygen

ity. Conclusion: It was isolated and partially characterized a lectin from the seeds of Mucuna pruriens approximately logical function. Keywords: Lectin; Mucuna pruriens; characterization CPE-1412b Prospecting Biochemistry and Pharmacology of Protein Fractions of Sterculia foetida Gadelha, C. A. A.¹; Santi-Gadelha, T.¹ 1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil 2. Program in Biochemistry – UFC, Brazil 3. Program in Nutrition Science – UFPB, Brazil Background: Currently the plants are still an unknown and

Phenylhydrazines. In erythrocytes treated with AEL, the

-

AEL showing that acts as antioxidant compound decreasSterculia foetida. Methodology: The seed meal has prolectin showed little relation to oxidant Phenylhydrazines tion of this agent. Keywords: Lectin; Abelmoschus esculentus; Oxidation

of type A, B and O, using the chemical Hemolyzing Tri-

CPE-1412a Isolation and Characterization of a Lectin from Seeds of Mucuna pruriens

us, causing you to read the results in a spectrophotometer

-

glutelin showed hemagglutinating activity. All fractions 1. Laboratory of Biochemistry, Genetics and Radiobiology – BioGer/UFPB, Brazil 2. Program in Biochemistry – UFC, Brazil 3. Program in Nutrition Science – UFPB, Brazil -

aeruginosa, Escherichia coli and Staphylococcus aureu. By SDS-PAGE showed the variety of protein fractions in seeds of Sterculia foetida. All protein fractions of the

lectin from the seeds of Mucuna pruriens. Methodology: were tested for the presence of hemagglutinating activity tographed on a column of molecular exclusion Sephadex G-100. All proteins were eluted protein displayed in their

logical use of its constituents. Keywords: Sterculia foetida; Pharmacology; Protein CPE-1412c seeds of Clitoria fairchildiana

pH, respectively. The presence of resistance to proteolytic melain and papain. The effect of chelating agent was determined using EDTA. Beta-mercaptoethanol and urea were used to determine the effect of reducing agents, as well as the metaperiodato sodium was used to verify the action of oxidizing agent. In all experiments, the hem-

1. Laboratory of Biologically Actives Molecules - Federal University of Ceará, Brazil 2. Laboratory of Biochemistry, Genetics and Radiobiology - Federal University of Paraíba, Brazil University of Ceará, Brazil

hemagglutinating. Results: A lectin was isolated from the Its molecular mass was estimated at 116 kDa. This protein well as any reducing agent. The presence of EDTA and

ity in the lectin-like from seeds of Clitoria fairchildiana. 25

Experimental Pathology and Health Sciences

Methodology: Paw edema model: C. fairchildiana (0.015, ic volume (mL) in a certain time interval after the stimulus -

CPE-1413a Behavioral assessment in wistar rats exposed to chronic treatment with different doses of ethanolic Ramalho, J.A.1; Lira, A.B1.; Dias, G.E.N.1; Alves,M.F.1;

-

L.S.N.1; Diniz, M.F.F.M.1; Medeiros, I.A1. 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brazil

for total and differential leukocyte counts. Results: The lecclinical toxicological study the ethanolic extract of Dioclea Conclusion: The lectin-like from C. fairchildiana presents -

the animals were realized fortnightly, through the open ticipated in the test for 3 minutes, for the evaluation of its

childiana CPE-1413 Assessment biochemical parameters of wistar rats towards chronic treatment with different doses of etha-

sexes the results were compared with the control group. In

Ramalho, J.A.1; Lira, A.B1; Oliveira,K.M.D1; Dias, malho, L.S.N.1; Medeiros,I.A1; Diniz,M.F.F.M.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brazil

-

lance (ANVISA), the ethanolic extract of Dioclea grandi-

extract. Conclusions: Throughout the experiment showed these effects.

days. In the end of the study, the animals were euthanized

-

evation of aspartate aminotraferase and lactic dehydrogenase in rats treated at all doses. Conclusions: Throughout the experiment, showed signs of chronic toxicity, mainly 26

CPE-1414 Morphometric analysis of the prostate in experimental DMBA administration Gustavo Barandas1, Eduardo Costa2, Felipe Pereira2, Rodrigo Farinha1 1. Experimental Pathology Service, Faculty of Medicine, University of Coimbra 2. Pharmacy Faculty, University of Coimbra nosuppressive action. This compound is found in cigarette engines. The present study aims to evaluate the action of DMBA on prostate using morphological parameters and morphometric analysis. quarantine period of one week. After that, they were ran-

2011 5 (2)

food handlers in a state nursery in the city of João Pessoa tum. At the end of the assay time, all animals were ethically killed and the complete necropsy performed. Fragments of prostate were collected for routine histopathological processing and examination. Pictures of routine staining slides were taken and the measurement of its follicles areas performed using the software ImageJ. The frequencies dis-

samples, including: 38 children and 25 adults. During the phase consisted of fecal examinations of the samples, using the methods of Hoffman and Faust. Later, it was performed the treatment of infected individuals. Results: Af-

These results allow us to think that any change induced CPE-1414a Morphometric analysis of the thyroid in experimental DMBA administration Gustavo Barandas1, Magali Jorge2, Felipe Pereira2, Silvério

ter the analysis of the results it was noticed a high percentage of infected individuals for more than one species of parasite, particularly those from fecal-oral transmission. Keywords: multiparasitism, prevalência, transmission

1

1. Experimental Pathology Service, Faculty of Medicine, University of Coimbra 2. Pharmacy Faculty, University of Coimbra mune system and has a carcinogenic action. The human exposure to DMBA is linked to several life events, such as smoking and exposure to atmospheres with exhaust gases

CPE-1415a Evaluation Of Antinociceptive Activity Of The Monoterpene Linalool Oxide In Mice Arruda, P.A.1, Souto-Maior, F. N.1,2, Salgado, P.R.R.1, W.B.1, Morais, L.C.S.L.1, Almeida, R.N.1 1. Laboratório de Tecnologia Farmacêutica - UFPB, Brasil 2. Universidade Federal de Campina Grande, Brasil Background: Linalool oxide (OXL) is a monocyclic alcohol

aims to evaluate the action of DMBA on thyroid using morphological parameters and morphometric analysis. reactions such as degradation product of linalool (Demytquarantine period of one week. After that, they were rantum. At the end of the assay time, all animals were ethically killed and the complete necropsy performed. Fragments of thyroid were collected for routine histopathological processing and examination. Pictures of routine staining slides were taken and the measurement of its follicles areas performed using the software ImageJ. The frequencies characterized. gration of the graph with more cases of larger follicles in the test group. These results allow us to conclude that thyroid is affected

experimental models. Methodology: Male Swiss mice, 25 to 35 grams, approximately 3 months, were divided in 5 min. after administration, the animals received 20 µL of a surface. Then, it was recorded the lick time (s) of the inthis same parameter was evaluated for 15 min (second phase). In the acetic acid induced writhing test ,thirty min after the administration, the animals received acetic acid

at doses of 100 and 150 compared to the control group. In at three doses (50, 100 and 150) compared to the control

follicles areas. decrease in latency at doses of 100 and 150 compared to CPE-1415 Prevallence Of Multiple Parasites In Children And Food Handlers In A State Nursery In The City Of João Pessoa - Pb Torres, P.A.1, Moura, J.A.P.1, Antunes, P.S.1, Martins, I.S.1, Rodrigues, D.A.1, Cardoso, B.C.1, Vieira, L. M.1, Rocha, 1. Universidade Federal da Paraíba, Brasil 2. Departamento de Ciências Farmacêuticas – UFPB, Brasil Background: The enteroparasite infections are still responmine the prevalence of multiple parasites in children and

compared to the control group. Conclusions: These results showed antinociceptive activity demonstrated in methodologies in vivo for analgesia. It is necessary others studies fects. Keywords: CNS, Linalool Oxide, Antinociceptive CPE-1416 Ibuprofen nanoparticles for oral delivery: characterization and in vivo toxicity evaluation 1, Fernandes, Figueiredo, I.V.2,5, Veiga, F.2,5 C.3, Candeias, S.4, Reis, C.3 1. Institute of Experimental Pathology, Faculty of Medicine - Uni27

Experimental Pathology and Health Sciences

versity of Coimbra, Portugal 2. Faculty of Pharmacy, University of Coimbra, Portugal 3. CBIOS – Laboratory of Nanoscience and Biomedical Nanotechnology (LNBN), Universidade Lusófona de Humanidades e Tecnologias, Portugal 4. CBIOS – Nutrition and Analitical Methods Unit (UNMA), Universidade Lusófona de Humanidades e Tecnologias, Portugal 5. Center for Pharmaceutical Studies (CEF), Faculty of Pharmacy – University of Coimbra, Portugal tory drug (NSAID) widely used as analgesic and antipyret-

prepared according to the traditional Peruvian medicine. and TLC analyses. We used the carrageenan-induced paw edema test to assess the effect of the PACs rich fraction in test was used to evaluate in vivo central analgesic activity and writhing test was used to evaluate in vivo peripheral -

spectives in pharmacology, it is intended, using this area, fen as a model drug. Methodology: Polylactic acid (PLA)

-

ments were collected from various organs and tissues. All

for positive control group of edema reduction. The results pain reduction. Although, results show that this fraction ing that the analgesic activity is related to the nociception tory process. CPE-1416b activities of Agrimonia eupatoria L. on in vivo models. Santos T.1,2*, Ferreira J.1,2, Francisco V.1,2, Costa G.1,2,

281.1 ± 66.7 nm and zeta potential was -4.3 mV. The not agglomerated. In respect to the analysis of histological results, there was a reduction of gastric toxicity when successful in terms of the development and characterization of the formulation. Moreover, there was a reduction

M.T1,2, Castel-Branco M.M.1,2, Figueiredo I.V.1,2 1 Faculty of Pharmacy, University of Coimbra, Portugal. 2 Center for Pharmaceutical Studies (CEF), University of Coimbra, Portugal. 3 Faculty of Medicine, University of Coimbra, Portugal. Introduction: Agrimonia eupatoria L. is used for the treatous studies indicate that the aqueous extract of Agrimonia presents a prevalence of several phenolic compounds and its ethyl-acetate fraction shows anti-oxidant activity and

now in progress, will test this strategy using other polyous extract of Agrimonia and its ethyl acetate fraction in animal models and to control their toxicity. Methods: The

Keywords: Nanotechnology, Polymer, toxicology CPE-1416a ties of Uncaria tomentosa (Willd.) DC proanthocyanidins rich fraction on in vivo models Ferreira J.1,2, Santos T.1,2, Francisco V.1,2, Costa G.1,2, M.T.1,2, Castel-Branco, M.M.1,2, Figueiredo I.V.1,2 1 Faculty of Pharmacy, Universty of Coimbra, Portugal. 2 Center for Pharmaceutical Studies (CEF), University of Coimbra, Portugal. 3 Faculty of Medicine, University of Coimbra, Portugal.

The hot-plate test and writhing test were used to study, respectively, the central and peripheral analgesic activities and the formalin test to complement these results. The tory activity. In all three tests we used test groups with two concentrations (doses) of the sample. For the toxicological assessment, we used the kidneys and livers of rats administrated with the samples. Results: The results for central analgesia indicates no activity. With the writhing test, we

oedema reduction. Finally, the histologic analysis does Discussion: These results prove the analgesic and antimatory and analgesic activities of a Proanthocyanidins rich 28

consequence of the activity, through several mechanisms, of the phenolic compounds in Agrimonia. Also, there are

2011 5 (2)

-

no toxic effects of Agrimonia on liver or kidneys. analgesic CPE-1419 Histopathological description of 132 rat tumours DMBA-induced 1 Calado, A1,2 1. Institute of Experimental Pathology, Faculty of Medicine - University of Coimbra, Portugal 2. Universitary School Vasco da Gama, Coimbra, Portugal

study was the assessment of systemic skeletal changes, nous healing. Methodology: A total of 18 Wistar male -

-

mary tumours in rats. There is a large consensus on histhe assayed period. Biochemical plasma analysis revealed This study relates the tumour malignancy potencial with tumors DMBA. Methodology: 48 Sprague-Dawley virgin

glucose, urea, alanine transaminase and ionized calcium, while ionized phosphor values diminished from 15 days

Kg) at 50 days and ethically euthanized after 26 weeks. A rats developed 132 mammary tumours, all collected and processed for routine haematoxylin-eosin histopathology. enomas and 127 carcinomas, 124 were invasive and 3 were terns present was: 42 tumours with single pattern and 57 had 2 patterns, 30 had 3 patterns and 1 had 5 patterns. Two groups, (1) 42 single pattern tumours and (2) 90 mixed pattern tumours, were considered for prevalence malignancy potential on characteristics like histopathological pattern, haemorrhage, tumour grading and stromal reaction. Reand papilar carcinoma in single patterns tumours and a cinoma in mixed pattern tumours. Single pattern tumours Necrosis, haemorrhage and stromal reaction were more were of low and medium grade, respectively. Conclusions: Mixed pattern tumours are more malignant than single pattern ones. On future work is important to validate the Keywords: DMBA, mammary tumours, histopathological evaluation CPE-1419a Assessment of skeletal systemic changes during local bone tissue regeneration Gomes PS1, Colaço B2, Pires2 MJ, Santos C3, Calado A4,5, Carmezim MJ3, Fernandes MH1 1 – Laboratório de Farmacologia e Biocompatibilidade Celular – Faculdade de Medicina Dentária, Universidade do Porto, Portugal 2 – CECAV. Universidade de Trás-os-Montes e Alto Douro. Portugal. 3 – Instituto Politécnico de Setúbal, EST, Estefanilha, Setúbal, Portugal. 4- Escola Universitária Vasco da Gama, Coimbra. Portugal. 5- Institute of Experimental Pathology, Faculty of Medicine - University of Coimbra, Portugal generation remains an important challenge. Nonetheless

showed an inverse correlation with plasmatic ALP activity. Biomechanical values diminished from 15 days (femurs and following increased at the 90 days time point (femurs -

Keywords: Bone tissue; Bone regeneration; biomechanics. CPE-1420 Protective Effects Of The Dipeptidyl Peptidase Iv Inhibitor Sitagliptin In The Blood-Retinal Barrier In A Type 2 Diabetes Animal Model Fernandes R 1, Gonçalves A 1, Leal E 2,3, Paiva A 4, Teixei1,3 ra-de-Lemos E 1,5, Teixeira F 1, Reis F 1 , 1

1. Pharmacology and Experimental Therapeutics, Institute of Biomedical Research in Light and Image (IBILI), Faculty of Medicine, University of Coimbra, Coimbra, Portugall; 2. Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; 3. Centre of Ophthalmology and Vision Sciences, IBILI, Faculty of Medicine, University of Coimbra, Coimbra, Portugall; 4. Histocompatibility Center of Coimbra, Coimbra University Hospital, Portugal; 5. ESAV, Polytechnic Institute of Viseu, Viseu, Portugal attenuate some of the complications of this disease. The

(ZDF) rats. Methods: ZDF rats at 20 weeks of age were treated with 29

Experimental Pathology and Health Sciences

Conclusions: The two analyzed materials have very distincNonetheless, in clinical practice these two materials are used in similar applications. The morphostructural propcells present in peripheral circulation and endothelial proistry.

dardized research is recommended to explore the full poKeywords: bone graft, SEM, porosity

CPE-1423 Dental erosion with lactic acid: in vitro studies levels of CD34+ cells in the peripheral circulation and

EPC to the retinal vessels. Conclusions: Sitagliptin prevented nitrosative stress, inrat retinas. Keywords: sitagliptin, blood brain barrier, diabetes

1. Experimental Pathology Service, University of Coimbra, Portugal 2. Pharmacology and Experimental Therapeutics, Institute of Biomedical Research in Light and Image (IBILI), University of Coimbra, Portugal teria, namely the Streptococcus mutans, plays a very important role on the appearance of carious lesions on the enamel surface. The initial stage caries is typically chardemineralization of the dental surface. On the other hand, and knowing that after intense physical exercise lactic acid teeth and other surrounding structures, we set out the hy-

CPE-1423 Morphostructural properties of two bone substitutes: differences between a xenograft and a synthetic material Guerra F.3 1. Experimental Patology Service, University of Coimbra, Portugal 2. Chemical Engineering Department, University of Coimbra, Portugal 3. Dentistry Department, University of Coimbra, Portugal Background: Bone grafts are widely used in surgical procedures in Dentistry, particularly in Periodontology, Implantology, Endodontics and Oral Surgery. Although autoge-

alization in the form of dental caries and dental erosion. Methodology: We exposed sixty eight third molars to four solutions of lactic acid with distinct pH values (3.5; 4.5; 5.5 and 6.8) during different periods of time (24 hours, 3 days, 7 days and 14 days). After the exposure, we measured water). Results: Only the solutions with pH values of 3.5 do not cause any kind of demineralization. It is not veri-

synthetic materials. A more future-oriented approach has -

were exposed to the same solution and in the same condi-

-

investigation on the dissolution dynamics of hard tissues (enamel and dentin) is needed in order to understand the

to analyze differences in what concerns morphostructural

Keywords: dental erosion, lactic acid, demineralization

CPE-1424 Metabolomic evaluation in a model for the Study of mastigatory muscles Rodrigo Farinha1, Marcolino Gomes2, Ludgero Tavares3, granules are more irregular, with sharp-edge tips while 30

1 Experimental Pathology Institute, Faculty of Medicine, University of Coimbra 2 Dental Medicine Area, Faculty of Medicine, University of Coimbra 3 Department of Biochemistry, Faculty of Sciences and Technology and Center for Neurosciences, University of Coimbra -

2011 5 (2)

tion, frequently associated with occlusal disarrangements. The role of the different muscles and the degree of involvement of each one in working and non-working sides, in this pathology, is not completely understood. derstanding of the changes in the masseter muscle in an experimental model of malocclusion.

to control, using paired Student “t” test. Conclusion: EOLs reduced stomach fundus smooth muscle contracted

groups: the control group, with the animals kept in the standard conditions and the test group, with a change of

studies pharmacological mechanisms. Keywords: L. sidoides; contractility; stomach.

composite on the right upper teeth. After 2 weeks, all animals, were ethically killed and the complete necropsy performed. Fractions of masseter muscle were collected for histopathological analysis and for high resolution magic angle spinning NMR spectroscopy (HRMAS) in a 14.1 Tesla Varian spectrometer. The morphometric analysis was performed using ImageJ software to measure the area

CPE-1427 Evaluation Of Toxicity Of The Hydroalcoholic Extract From The Stem Bark Of Pradosia Huberi On Male Rats Vital Organs

-

F.L.P.1; Rocha, A.O.B1; Medeiros, A.A.N.2 ; Medeiros, F.A.2; Diniz, M.F.F.M.3; Sá, R.C.S.4 1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB; 4. DFP/CCS/UFPB

phometric analysis presented a higher average area of the medicine to treat gastritis and gastric ulcer. The presence to the right muscle, where this area is smaller on test group. statistical analysis demonstrated that aminoacids glutamate and aspartate as well as lipids were incremented in the right masseter of test group while choline was decreased.

investigate the toxicity of the hydroalcoholic extract of P. Adult Wistar rats were divided into control and three treat-

glutamate and aspartate as well as lipids. This analysis algavage and once daily, for 63 days. The animals were killed rotomy, the following organs were removed and weighed:

3D projection of principal components. masseter muscles on working and non-working sides, which allows the separation of the cases. Although with no

of the masseter muscle on this experimental model, with weight of the kidney of the animals treated with PhHE transverse cuts. Keywords: Metabolomics, Morphometrics, Mastigatory muscle, Occlusal interference, Temporomandibular dysfunction CPE-1426 Effect of Lippia sidoides essential oil on the stomach smooth muscle contraction Arruda, I.T.S.1 ; Medeiros, M. A. M. B.1 ; Nascimento, ; Souza, E.P.1 1. Laboratório de Bioquímica Molecular- DBM/UFPB, Brasil. 2. Laboratório de Tecnologia Farmacêutica – LTF/UFPB, Brasil. 3. Universidade Federal do Ceará – UFC, Brasil. Introduction: Lippia sidoides, a medicinal plant popularly known as rosemary pepper, presents thymol as the main component of essential oil. Thymol affects smooth muscle fects of essential oil of L. sidoides (EOLs) on the contractility of the stomach fundus. Methods: Stomach fundus of the rats were dissected, sectioned and suspended in a tissue cumulative doses of essential oil of L. sidoides (EOLs) dissolved in DMSO in concentrations of 1, 3, 10, 30, 100

when compared to the control group (right 0.35 ± 0.01, in the relative weight of these two groups, suggesting the

tion to the control group (398.1 ± 5.6 g). Conclusions: These results suggest that the hydroalcoholic extract of P. of rats. Keywords: Pradosia huberi; toxicity; vital organs. CPE-1427a Stem Bark Of Pradosia Huberi On The HematologiF.L.P.1; Rocha, A.O.B1; Xavier, A.L.1; Pita, J.C.L.R.1; Medeiros, A.A.N.2 ; Diniz, M.F.F.M.3; Sá, R.C.S.4 1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB; 4. DFP/CCS/UFPB azonian species, is popularly known as “casca-doce” and used in the folk medicine for the treatment of gastritis.

ing vehicle. Data (mean ± standard error of mean) were -

Evaluate hematological parameters of male Wistar rats under the effect of different doses of hydroalcoholic extract 31

Experimental Pathology and Health Sciences

which the animals received water and PhHE (T1 - 1.22, (EDTA). The following hematological parameters were

CPE-1428a Evaluation Of The Fertility Of Male Wistar Rats Treated With The Hydroalcoholic Extract Of Pradosia Huberi P.S.1; Rocha, A.O.B1; Medeiros, A.A.N.2 ; Medeiros, F.A.2; Diniz, M.F.F.M.3; Sá, R.C.S.4 1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB; 4. DFP/CCS/UFPB nities in the treatment of gastritis. Bioactive compounds

(41.30 ± 1.53), T1 (42.69 ± 1.40), T2 (42.32 ± 1.36), T3 (42.83 ± 1.15). The WBC and platelets also showed no

jectives: To investigate the effects of the PhHE on the fertility of male rats mated with untreated females, which later had pregnancy interrupted on 15th gestational day. Methodology: Male rats were divided into control and

(7.21 ± 0.66), T1 (6.27 ± 0.5), T2 (5.57 ± 0.59), T3 (6.90 T1 (626.20 ± 55.39), T2 (703.80 ± 43.60), T3 (701.27 ± 24.45). Conclusions: The administration of the PhHE

days. Each rat was mated with two virgin females from the 57th to 63th day of treatment. The presence of sperm in vaginal smear was considered indicative of insemination (1th day of gestation). Pregnant females were monitored

Keywords: Pradosia huberi; rats; hematological parameters.

monitored throughout the gestational period (21 days) and the other had pregnancy interrupted on the 15th day. The occurrence of external malformations and the following reproductive index were analyzed: mating, gestation, preimplantation loss, implantation and resorption. Results: The administration of the extract did not interfere with

CPE-1428 Effect Of The Hydroalcoholic Extract Of Pradosia P.S.1; Rocha, A.O.B1; Xavier, A.L.1; Pita, J.C.L.R.1; Medeiros, A.A.N.2 ; Diniz, M.F.F.M.3; Sá, R.C.S.4 1. PgPNSB/CCS/UFPB; 2. IEPA; 3. DCF/CCS/UFPB; 4. DFP/CCS/UFPB

for presence of sperm in the vaginal smear (mating in-

of PhHE, respectively. The gestatation, resorption indexes the Amazon, used in folk medicine to treat gastritis. The ogy: Adult Wistar rats were divided into control and three -

These results indicate that the PhHE may impair fertility due to increased pre-implantation losses and reduced implantation, suggesting the occurrence of mutagenic effects on Wistar rats. Keywords: Pradosia huberi; fertility; rats. CPE-1430 Oesophagus morphometric analysis in experimental administration of semicarbazide Costa E1, Ramos A2, Capela-Silva F2, Marques P1,

for measurement of glucose, urea, acid uric, creatinine, transaminase and aspartate transaminase. Results: The (T3 - 2.7 ± 0.05). Although some parameters were high,

1.Faculdade de Farmácia da Universidade de Coimbra 2.Universidade de Évora 3.Faculdade de Medicina da Universidade de Coimbra, Serviço de Patologia Experimental pact on some populations with economic restrictions. This tor of lathyrism. The aim of this study is to evaluate the oesophagus morphologic changes in the administration

(1.3 ± 0.3). Conclusions: The administration of PhHE did of the serum parameters measured in these animals. Keywords: Pradosia huberi; rats; biochemical parameters. 32

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2011 5 (2)

and the complete necropsy was performed. The fragments collected in the necropsy were studied under light microscopy. The medium third oesophagus transversal cuts slides lumen and the epithelial compartment areas. In the group I the epithelial compartment has a value of 228.27 ± 38.74 µm2 and the lumen 245.59 ± 28.78 µm2. In the group II the epithelial compartment has a value of 179.2 ± 42.69 µm2 and the lumen 118.25 ± 71.0 µm2. In the group III the epithelial compartment has a value of 189.90 ± 65.33 µm2 and the lumen 100.66 ± 39.0 µm2. These results allow us to conclude that the lumen area of oesophagus is

water and Tween 80, the experimental groups, IM-3. In Rota-rod test, the animals were treated and after 30, 60 and 120 minutes of administration were placed in the device. utes. In the elevated plus maze test, exploration capacity of entries and total time spent in open arms and closed for 5 minutes. Results: In the Rota-rod test, there was not vs. 179.3±0.7), 60 (50: 162.4±15.5; 100: 159.4±18.8; 200

changes were detected on the epithelium. Keywords: Semicarbazide; Lathyrism; Oesophagus. vs. 173.0±6.0) after treatment. In the elevated plus maze CPE-1431 of entries in open arms (3.2±0.6, 2.2±0.8 and 2.6±1.0, respectively vs. 2.5±0.8). The stay time in the open arms Carvalho, A.S.1; Martins, I.S.1; Antunes, P.S.1; Sales, I.R.P.1; Vasconcelos, W.P.1; Nascimento, R.F.1; Silva, R.O.1; Oliveira, K.H.S.P.1; Araújo, I.K.M.1.; Freitas,F.I.S1 1. Departamento de Ciências Farmacêuticas – Universidade Federal da Paraíba, Brasil Background: With regard to parasites, although high rates are most often detected in developing countries, little is

kg: 35.2±12.4 vs. 41.2±14.3). Conclusions: The IM-3 does anxiolytic drugs, according to the elevated plus maze test. Keywords: IM-3, derived imidazolidine, central nervous system

has own characteristics and transient standards of metaCidade Verde. Methodology: The records of 56 pregnant soa - PB were analyzed, and from them were registered the results of parasitological examinations performed during pregnancy. Results: Given what was analyzed was found

CPE-1432a Darier Disease (Dd) – Differential Diagnosis From The Pathological Exam Paulo, L.L.1; Silva-Netto, L.M.S.2; Sousa-Neto, F.F.2; Leite, ga, R.M.1; Mangueira, V.M.1 1. Universidade Federal da Paraíba, João Pessoa-PB, Brasil. 2. Faculdade de Ciências Médicas da Paraíba, João Pessoa-PB, Brasil. Introduction: DD is a rare autosomal dominant genodermatosis of chronic course that presents changes in the keratinization of the epidermis, mucous and phanero. It -

-

the DD of the others acantholytic dermatoses, from the pathological examination. Methods: It was made a clinical

infection in pregnant women, and this low prevalence is them during pregnancy. Keywords: Pregnants; parasites; immunology. CPE-1432 Evaluation Of Locomotor And Anxiolytic Activity Of Im-3 R.M.1; Monte, L.O.1; Souza, S.A.2; Almeida, R.N.1; Morais, L.C.S.L.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, PB, Brasil. 2. Departamento de Química, Universidade Federal da Paraíba, João Pessoa-PB, Brasil. Introduction: The fact of IM-3 has a similar hydantoins structure, known for its effects on the central nervous system (CNS), increase the intention to analyze the effects using rodents. Methodology: In the Rota-rod and elevated grams and divided into 8 groups. The administrations

and from this it was made the pathological exam. Results: Originally appeared in the anterior face chest with gradual worsens with sun exposure. The dermatological examination showed papules and plaques keratotic scaly, erythematous, in the anterior chest, cervical region, face and upper epidermis with focus of hyperkeratosis, dyskeratotic kerasion: From these results it can has a differential diagnosis, concluding that it was the DD. So, we can guide the patient favors the non-appearance of secondary infections. Keywords: DD, pathological exam, differential diagnosis CPE-1433 The Pharmaceutical Care Exist? A Timely Analysis Of An Integrated Unit Of Health In João Pessoa – Pb Galvão, J.G.F.M.1; Antunes, P.S.1; Martins, I.S.1; Sales, I.R.P.1; Vasconcelos, W.P.1; Nascimento, R.F.1; Silva, R.O.1; Oliveira, K.H.S.P.1; Araújo, I.K.M.1.; Freitas,F.I.S1 1. Departamento de Ciências Farmacêuticas – Universidade Federal 33

Experimental Pathology and Health Sciences

da Paraíba, Brasil Background: Pharmaceutical care is an interdisciplinary tem, involves the supply of drugs provided to the user to ensure the rational use of medicines. The pharmacist is 166.5±3.6 vs. 179.1±0.4) after treatment. Conclusions: at improving access and promoting rational use of drugs, for the full development of pharmaceutical care. But in our reality, the pharmacist is not inserted in the process of primary health care, not only causing losses to the comvided at the clinic. Methodology: For this study, we used in home visits occurred during the fourth stage of expeWe cut out the four questions on the form, according to health care unit. As the process of dispensing drugs only

that the presence of the pharmacist in the unit improve in some way, the services offered. Conclusions: Thus, it

activity to psycholeptics drugs, without compromising the motor coordination of animals in the Rota-rod test, deKeywords: HPA-05, derived imidazolidine, central nervous system CPE-143 Evaluation of antimicrobial and wound healing activities of the extract of Hymenaea stigonocarpa Mart. ex Hayne Lima, A F 1; Santos, F H P 1; Linhares, L A 2; Dimech, G S 2; Chaves, C 2; Nascimento, M S2; Maia, M. B. S. 2; Ximenes, E. A.2 1. Faculdade Integrada Tiradentes, Brazil, 2. Universidade Federal de Pernambuco, Brazil Introduction: The species Hymenaea stigonocarpa Mart. ex Hayne, Leguminosae-Caesalpinioideae commonly macology of the genus Hymenaea several researchers The aim of present study was to evaluate the in vivo an-

primary health attention and pharmaceutical care services 28 strains of Staphylococcus aureus with phenotypes of Keywords: Pharmacist; Primary Health Attention ; Users. PE-1434 Preliminary Psychopharmacological Study Of Imidazolidine Derived Hpa-05

used the wound mode in rats. Wounds were infected with a Staphylococcus aureus Methicillin resistant strain and treated, once a day, with the H. stigonocarpa hydroalco-

D.V.¹; Paulo, L.L.1; Souza, S.A2; Almeida, R.N.1; Morais, L.C.S.L.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, PB, Brasil. 2. Departamento de Química, Universidade Federal da Paraíba, João Pessoa-PB, Brasil. Backgroung: HPA-05 is an imidazolidine derivative, structurally similar to others of the same class, whose activities in the central nervous system was reported in previous

of animals treated with the H. stigonocarpa gel healed at the end of the experiment. Healing rate of treated with the H. stigonocarpa gel was higher than controls and this

of HPA-05 in animal models, using rodents. Methodology:

also tissue formation in more advanced stage of maturation than controls. Conclusion: More studies on the active ment of a new drug. Keywords: Hymenaea stigonocarpa, S. aureus, healing. CPE-1438 Effect of a low-protein diet during pregnancy on pro-

received distilled water and tween 80, the experimental ment, the animals were placed individually in the center of eters. In Rota-rod test, the animals were treated and after 30, 60 and 120 minutes of administration were placed in -

34

Xavier,T.F. 1, Silva, K.O.G. 2, Almeida, L.C.A.2, Canon, F3, Manhães-de-Castro,R.4,Toscano, A.E.5 1. Centro de Ciências Biológicas,UFPE,Recife/PE 2. Departamento de Fisioterapia,UFPE,Recife/PE 3. Université de Technologie de Compiègne/França 4. Departamento de Nutrição,UFPE,Recife/PE 5. Centro Acadêmico de Vitória/UFPE, Vitória de Santo Antão/PE Background: The protein-calorie malnutrition during the critical period of development can alter the function of all systems in the long run. In the muscular system, peri-

2011 5 (2)

(DBR). Methodology: Male Wistar rats were divided into -

Keywords: Nanuza plicata, cytotoxicity, antitumor. CPE-1440 Cytotoxic Activity of the Compound A398 In Tumor and Non-tumor Cell Lines Silveira, A. L.; Araújo, D.A.M. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brasil Background: The Podophyllotoxin is a lignan found in

for many years for its potent cytotoxic activity. Due to The images of muscle cells were recorded using computer image analysis system Leica QWin. The counting of muswas measured and compared using the Student t-test (Sig-

portance as a chemical model in the development of new fects of A398 (a new synthetic derivative of podophylof Industrial Property, INPI) in human colon tumor cells determination if this agent differentially could affect cytotoxic activities in hepatic tumor cell lines (HepG2) was also investigated. Methodology: We initially compared the

These changes can affect muscle function in the short and long term. CPE-1439 Evaluation of the cytotoxic effects of ethanol extract from leaves of Nanuza plicata Mangueira, V.M.1; Viana, W.P.1; Xavier, A.L.1; Pita, V. S.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brazil loziaceae. It is a species rarely reported in the literature tumor and toxicological activity in vitro of ethanol extract of Nanuza plicata (NPE) in K562 tumor cells and mice erythrocytes, respectively. Methodology: To in vitro antitu-

lines over a concentration range of 5 – 500µM using two tests, MTT reduction and neutral red assays. In HepG2 ence of dexamethasone (CYP3A inducer), ketoconazole tion). After 24 and 72 hours of treatment we do the cell

spectively, with 24h of treatment. This compound showed

of A398 in HepG2 cells. Pretreatment with dexamethasone decreased the cytotoxicity of A398 (IC50>250µM). Conclusions: A398 was more cytotoxicity in HT29 than in FGH and HepG2 cells. In addition, hepatocyte protection the CYP3A isoforms and reactions of glucuronidation in Keywords: Cytotoxicity, cancer, metabolism

for 72 h. In 96-well plate with cells under 72 h of treatsupernatant was carefully removed and added 100 µL of read at 595 nm. To in vitro toxicological activity Swiss mice -

pernatants was read at 540 nm to determine the concenNPE showed an in vitro antitumor activity with IC50 value

that NPE has in vitro antitumor activity and low toxicity against erythrocytes, cells commonly affected in the treatments with anticancer agents.

CPE-1441 Involvement Of Potassium And Calcium Channels In Tocolytic Effect Of Galetin 3,6-Dimethyl Ether On Rat Uterus G.A.1; Travassos, R.A.1; Souza, I.L.L.1; Queiroga,F.R.1; Lira, D.P.1; Santos, B.V.O.2; Silva, B.A. 2 1 Laboratório de Tecnologia Farmacêutica, 2 Departamento de Ciências Farmacêuticas, UFPB, João Pessoa/ PB. ceae) is popularly known in northeast Brazil as “juremaspasmolytic effect on rat aorta and guinea-pig trachea through modulation of K+ channels that, indirectly, can ation. FGAL showed spasmolytic activity on rat uterus tives: To investigate the mechanism of tocolytic action of

35

Experimental Pathology and Health Sciences

CO2 mixture and resting tension of 1 g. Isometric and isotonic contractions were registered. All the experimental FGAL relaxed the rat uterus pre-contracted with 60 mM ± 0.1, respectively). We decided to investigate if FGAL

J.I.V.1; Filho, G.G.A. 1; Alves, H.S. 2; Chaves, M.C.O. 1; Pessôa, H.L.F. 3 1. Universidade Federal da Paraíba, Laboratório de Tecnologia Farmacêutica Prof. Delby Fernandes de Medeiros, João Pessoa-PBBrazil, Cx. Postal 5009. 2. Universidade Federal de Campina Grande, CEP 58540-000, Sumé-PB, Brazil. 3. Universidade Federal da Paraíba, Laboratório de Bioquímica, Genética e Radiologia, Departamento de Biologia Molécular, João Pessoa-PB-Brazil.

cumulative curves of CaCl2 in the presence of FGAL (106 - 10-5 M), was shifted to the right with a decrease maxilites, aiming to highlight the presence of new compounds pation of channels of K+ in this effect. Conclusions: The relaxant effect of FGAL in rat uterus involves positive

and hemolytic activities of the ethyl acetate fraction and Methodology: The plant material of Piper montealegreanum was collected in the municipality of Belém, State

Keywords: Galetin, spasmolytic, uterus CPE-1442 Evaluation Of The Toxicity Of Pradosia Huberi Hydroalcoholic Extract On The Hypophysis-Gonadal Axis Of Pregnant Female Rats Rocha, A.O.B1; Souza, L.Q.S.1; Sousa, T. M1; Figueiredo, R. R.1; Potiguara, R. K. S1.; Pita, J. C. R1; Santos, E.C.S.1; Medeiros, A.A.N.2;Diniz, M.F.F.M.3; Sá, R.C.S.4 1. Program of Postgraduation in Natural and Syntactic Bioactive Products, Center of Health Sciences – LTF/UFPB, Brazil 2. Institute of Technological Research of Amapá - IEPA, Brazil 3. Department of Pharmaceutical Sciences, Center of Health Sciences - UFPB, Brazil 4. Department of Physiology and Pathology, Center of Health Sciences - UFPB, Brazil dicinal plant commonly found in the Amazonian forest and popularly used to treat gastritis and gastric ulcer. The jectives: In this study, the toxicological effect of the hywas investigated on the organs of the reproductive axis (hypophysis, ovaries and uterus) of pregnant female rats.

(LH-20) column chromatography and their structures 13C NMR-500 and 125 MHz, respectively, IR and ESIfrom the genera Bacillus, Escherichia, Pseudomonas and -

cytotoxic effect. Results: The ethyl acetate fraction showed resistant to ampicillin at lower concentrations showing no cytotoxic effect against human erythrocytes (A, B and O). ampicilin and were not cytotoxic as well. Conclusions: The -

four groups (containing 15 or 16 animals each), in which Keywords: Piper montealegreanum, antibacterial activity, cytotoxicity. of gestation (preimplantation phase). The animals were tion. After laparatomy, the following organs were removed ovaries and uterus. Results: The relative weight of the hy-

CPE-1444 Low frequency of intestinal parasites in inhabitants of Sousa city – Paraíba – Brazil Souza, L.Q. 1; Figueiredo, R. R.1; Monteiro, J.C. 1; Pereira, J.C. 1; Leite, F.V.P1; Pequeno, I.F.P. 1; Lima, C.M.B.L. 1; Cavalcanti, M.G.S. 1; Freitas, F.I.S. 1. 1. Department of Pharmaceutical Sciences, Center of Health Sciences - UFPB, Brazil

0,001) on the 8th day on gestation. The other parameters hypophysis, which could interfere with the normal functioning of the hypophysis-gonadal axis. Keywords: Pradosia huberi; reproductive toxicity; hypophysis-gonadal axis. CPE-1443 Antibacterial and hemolytic activities from Piper Montealegreanum Yuncker (Piperaceae) Pinto, D.S.1; Duarte, F.M.1; Delmondes, M.N.1; Costa 36

eradication of these parasites requires improvement of socio economic, sanitation and health education. Aim: Investigate the frequency of intestinal parasites of Sousa -

From 3921 tests performed, only 420 were positive for at -

2011 5 (2)

thes. The most frequent parasite was Endolimax nana , fol-

quiring toxicity studies. Croton grewioides species is not reported in the literature with regard to its pharmacologi-

The compiled data suggest that the studied community is in a context that favors the maintenance and spread of

acute toxicity of essential oil from leaves of C. grewioides (O.E.C.) in mice. Methodology: For the acute study, the O.E.C. was administered in mice (i.p.) at doses of 250, 375

sample, along with the use of a single diagnostic technique, greatly reduces the chances of detecting the parasites. Keys-word: intestinal parasites, low frequency, Sousa PE-1445a In vivo antitumor activity and toxicity of essential oil from leaves of Lippia microphylla Cham. Batista, T.M.1; Sousa, T.K.G.1; Viana, W. P.1; Pessoa, D.R.1; Xavier, A.L.1; Pita, J.C.L.R.1; Silva, T.G.2; Tavares, J.F.1; Silva, M.S.1; Castello-Branco, M.V.S.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brazil 2. Departamento de Antibióticos, Universidade Federal de Pernambuco, Brazil Background: Many anticancer drugs currently used in But natural products, as anticancer agents, can also cause damage to the organism, requiring toxicity studies. Lippia the in vivo antitumor activity and toxicity of the essential oil from leaves of Lippia microphylla (OEL). Methodology: Eight-day-old sarcoma 180 ascites tumor cells (25 x 7 days. To the negative control group was administered was evaluated the food and water consumption, as well the matological analysis, and then all animals were euthanized. Liver, kidneys, spleen, thymus and tumor were excised and weighed. Results: The in vivo antitumor study demonstratanalysis showed moderate gastrointestinal and hematological toxicity. Alterations in liver function was also evidenced

widely anticancer drugs used in clinical medicine. Conclu-

and mortality were determined for up to 14 days. Indexes of organs (liver, heart, kidneys, spleen and thymus) were progressively with increasing doses. Some adverse effects, such as sedation, anesthesia, decreased response to touch, administration and were more pronounced at higher doses in the indexes of heart, liver, kidneys, spleen and thymus

mals. Conclusion: O.E.C. showed moderate acute toxicity when administered i.p. to mice. The LD50 value is used conducted. Keywords: Croton grewioides; acute toxicity; essential oil. CPE-1448a Evaluation of the antitumor activity and toxicity of essential oil from leaves of Rollinia leptopetala RE Fries Mangueira, V.M.1; Brito, M.T.1; Batista, T.M.1; Moreira, M.M.B.1; Sousa, T.K.G.1; Moura, A.P.G.1; Lunguinho, D.M.1; Tavares, J.F.1; Diniz, M.F.F.M.1; Castello Branco, M.V.S.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba Background: Rollinia leptopetala R.E. Fries (Annonaceae), ate the in vivo antitumor activity and toxicity of essential oil from leaves of Rollinia leptopetala (E.O.R.). Methodology: An acute toxicity test (LD50) was performed to determine the doses of in vivo antitumor test. To in vivo antitumor activity, eight-day-old sarcoma 180 ascites tumor cells

vivo with moderate toxicity, which is not a limiting factor Keywords: Lippia microphylla; antitumor activity; toxicity. CPE-1446 Acute toxicity of essential oil from leaves of Croton grewioides Baill. (Euphorbiaceae) in mice Sousa, T.K.G.1; Batista, T.M.1; Moreira, M.M.B.1; soa, D.R.1; Lunguinho, D.M.1; Tavares, J.F.1; CastelloBranco, M. V.S.1 1. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brasil Background: Medicinal plants represent the largest source ral products can also cause damage to the organism, re-

were euthanized and tumors and organs were excised and and food and water consumption. Results: The LD50 tively. In O.E.R. treated groups, the toxicological analysis increase in AST level and decrease in MHC and MCV with 37

Experimental Pathology and Health Sciences

increase of neutrophils, when compared to the control group. The decrease in consumption of water and food

tumoral. However its effects on the immune system are powder of Pc seeds (Ps) and its main active principles caffeine (Caf) and catechin (Cat), in the cellular immune re-

activity in vivo and moderate toxicity in experimental models evaluated. Keywords: Rollinia leptopetala; antitumor activity; toxicity. CPE-1449 Pharmacological properties of Artichoke (Cynara scolymus) extracts using thin-layer chromatography bioautography assay (TLC) Mattos, M.I.S1; Hansen, D2; Haraguchi, M2; Sartori, 1. Departamento de Patologia Experimental e Comparada, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo – USP – Brazil 2. Centro de P&D de Sanidade Animal, Instituto Biológico – SP - Brazil 3. Núcleo de Fisiologia e Bioquímica de plantas, Instituto de Botânica – SP - Brazil methods, is an effective and inexpensive technique for the study of plant extracts. Artichoke extracts are used for the documented in a wide range of experimental and clinical the different extracts and fraction from leaves of the aresterase activities using TLC. Methodology: TLC of the methanol extract and the hexane and dichloromethane Nystatin, quercetin and physostigmine were used as standards for antifungal, antioxidant and anticholinesterase cladosporioides to verify the presence of fungitoxic zones; other two plates were sprayed with DPPH (2,2-diphenyl1-picryl hydrazyl) and acetylcholine enzyme (AChE) for antioxidant and anticholinesterase tests. The activity of romethane extract showed highest anticholinesterase activity and moderate antifungal activity. The hexane fraction ity. Conclusions: These results demonstrate that artichoke extracts have a marked antioxidant, antifungal and anticholinesterase activities and TLC is an easy and fast assay for these tests. Further research is needed for the safe use of these extracts. Keywords: Artichoke, Thin-layer chromatography. CPE-1449a Guarana, due to catechin, decreases the delayed-type hypersensitivity (DTH) response Caniceiro, B.D.1; Latorre, A.O.1; Carvalho, L.R.2; Naka1. School of Veterinary Medicine and Animal Science - University of Sao Paulo, Sao Paulo, Brazil 2. Botany Institute of Sao Paulo, Sao Paulo, Brazil Introduction: Seeking for immunomodulatory compounds with therapeutic potential is constant due to the huge vaimmunological responses. In this context, it is known that Paullinia cupana (Pc), popularly called guarana, is a 38

G (Ps), CF (Caf), CT (Cat) and FT (Caf + Cat). On Day taneous (sc) sensitization with 50 µl of 1x108 sheep red adjuvant solution). After 7 days, the diameter of the left hind footpad was measured (0 hour). The mice were then PBS only) into the left hind footpad. The increase in the footpad diameter was measured 24, 48 and 72 hours later. The swelling was calculated as the difference in diameter at 0 vs. 24, 48 or 72 hours and expressed in millimeters. Rethe CT group after 48 hours and in the mice from G, CT and FT groups after 72 hours. Conclusion: These results

Keywords: guaraná; catechin; DTH. CPE-1450 Importance Of Infection By T- Lymphotropic Virus - Virus Type I (Htlv-I) In Connection Vertical Transmission Machado, W.G.S.1; Santos, A.H.1; Pereira, B.D.S.1; Silva, S.R.1; Cardoso, H.M1; Gomes, A.M.P.1,2; Nunes, M. K. M.M.S.,4. 1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil 2. Federal University of Paraiba, Brazil. 3. Oral Biology Laboratory, Health Science Center, Federal University of Paraiba, Brazil 4. Biological Science Center, State University of Paraiba, Brazil Introduction - The human T-lymphotropic virus I (HTLV-

adults (ATL). He is also related to other non-malignant -

of this virus in pregnant women, specifying ways to diagnose and valuing the use of primary prevention measures to control the spread of the virus, especially for prophylaxis of vertical transmission. Methodology - This study The HTLV-I is a retrovirus with oncogenic potential that viduals, usually 20 to 30 years after infection. HTLV-I is not easily transmitted, as the cell-cell contact is necessary. In Brazil it is mandatory to carry out the tests to track the

2011 5 (2)

the prophylactic measures for vertical transmission are efKeywords: lymphotropic virus Virus type I; Vertical Transmission; leukemia / lymphoma. CPE-1452

study. We will analyze Giemsa and Papanicolaou-stained slides of patients diagnosed positive for malignant cells records of these patients, who underwent examinations

and History of Breast Cancer Santos, G.T.; Zavalhia, L.; Camillo, N.D.; Brackmann, R.; Prolla, J.C.; Bica, C. 1 – Post Graduation in Pathology - Universidade Federal de Ciências da Saúde de Porto Alegre, Brasil. 2 – Graduation in Medicine - Universidade Federal de Ciências da Saúde de Porto Alegre , Brasil. 3 – Laboratory of Pathology of Complexo Hospitalar Santa Casa de Porto Alegre/RS, Brasil. This is a historical cohort study, in which we investigated tistical analysis was performed using Stata. Version 9.0., Kaplan-Meier method to analyze survival. Results: We analyzed 145 patients with suspected malignant pleural effu-

of malignant pleural effusion and death. In patients with

we compared its expression in relation to progression to death, and we noted that there was a higher occurrence of death in the groups that did not express ER and PR and

related to the time of appearance of pleural effusion and the pattern of survival. Results and Conclusion: Among months. Our preliminary data indicate that, among the 27 mean survival was 3.1 months. Among the 27 slides anavival in this case was 3.5 months. Screening and analysis of the slides of these patients with malignant cells are in progress. Keywords: breast cancer, pleural effusion, cell morphology. CPE-1453 Evaluation Of The Expression Of C-Kit (Cd117) In Ependymomas And Oligodendrogliomas Childhood And Adults Zavalhia, L.S.1; Santos, G.T.1; Romitti, M.2; Meurer, R.T.3; 1 Federal University of Health Sciences of Porto Alegre (UFCSPA), Brasil 2 Federal University of Rio Grande do Sul of the Rio Grande do Sul (UFRGS), Brasil 3 Laboratory Research Pathology of Federal University of Health Sciences of Porto Alegre (UFCSPA), Brasil 4 Department of Pediatrics of the Irmandade Santa Casa de Misericórdia of Porto Alegre (ISCMPA), Brasil 5 Department of Clinical Medicine of the Irmandade Santa Casa de Misericórdia of Porto Alegre (ISCMPA), Brasil Introduction: Oligodendroglial and ependymal tumors are a rare group of neoplasms of the central nervous system with multiple clinical manifestations, affecting children and young people and adults, respectively. Currently, stud(CD117) can target the treatment to the use of selec-

with expression of this tumor marker. Conclusions: This evaluate the expression of c-kit (CD117) in ependymomas and oligodendrogliomas and to estimate its usefulness Keywords: breast cancer, pleural effusion, survival. Methods: Specimens of ependymomas and oligodendroCPE-1452a Effusion in Patients with History of Breast Cancer 1.UFSCPA – Graduation – Medicine; 2 UFCSPA – Post Graduation – Pathology; 3.Complexo Hospitalar Santa Casa - Porto Alegre/RS – Laboratory of Pathology. Introduction: Breast cancer has the highest rate of cancer mortality among women. Pleural effusion frequently appears during the course of the disease, and the emergence

immunoreactivity using anti-c-kit (CD117, DAKO, dilution 1:50). Were used as positive control gastrointestinal stromal tumors. Results: The sample consisted of 34 ependymomas e 21 oligodendrogliomas. The median age at diagnosis was 6.5 (1-65) years for ependimomas and was 38.7 years ± 16:52. for oligodendrogliomas. The analysis immunohistochemistry demonstrated protein expression for CD117. However, in oligodendrogliomas was showed

spread from the primary tumor, which reduces the life ex39

Experimental Pathology and Health Sciences

tial proportion of ependymomas and oligodendrogliomas Keywords: Ependymomas; oligodendrogliomas; ckit. CPE-1454 Anti-spasmolytic effect of essential oil of Lippia sidoides on isolated guinea pig ileum. Arruda, I.T.S.1 ; Medeiros, M. A. M. B.1 ; Nascimento, R. Silva, B.A.2 ; Souza, E.P.1 1. Laboratório de Bioquímica Molecular- DBM/UFPB, Brasil. 2. Laboratório de Tecnologia Farmacêutica – LTF/UFPB, Brasil. 3. Universidade Federal do Ceará – UFC, Brasil. Introduction: Thymol, the major constituent of essential oil of Lippia sidoides (EOLs) folkly known as “rosemary pepper” has anti-spasmolytic effect in some smooth musEOLs on guinea pig ileum smooth muscle. Materials and tive doses of EOLs dissolved in DMSO in concentrations

strongly interlinked. It was found that regions where there is increase in mitosis predisposes the formation of atypical

the typical areas. Keywords: glioblastoma, angiogenesis, mitoses. CPE-1456 Sexual Orientation,Prevention of STDs, and Contraception Among Adolescents: First Observations on a Public School in João Pessoa-PB Rodrigues, M. L.¹ ; Leal, E.B.N. ¹ ; Martins, L.L ¹; Estevam, 1. Molecular Biology laboratory, College “Santa Emilia de Rodat”, Brazil 2. Master of Bioactive Natural and Synthetic Products -UFPB, Brazil 3. Graduating Student of Biomedicine “Santa Emilia de Rodat”, Brazil 4. College of Biomedicine “Santa Emilia de Rodat”, Brazil 5. Coordinator of “Bemfam” São Luis-MA, Brazil to start sexual life earlier, which ultimately increases the chances of so-called “sexual accidents.” It is worthy to -

-

fect to the control group. Conclusion: EOLs reduced contraction of guinea pig ileum smooth muscle treated with ther studies on the relaxing pharmacological mechanism. Keywords: L. sidoides; contractility; ileum.

themselves from sexually transmitted diseases (STDs) and unwanted pregnancies. Methodology: We performed video lectures and a data collection through a questionnaire containing questions such as: (When was the 1st intercourse, what they thought of contraception such as condoms, pills, injections). Applied to 20 students in 9th grade of the Municipal School “Violeta Formiga” located in Mandacaru, João Pessoa- PB. Results: The 20 students

CPE-1455 of glioblastomas Pinto, A. P. S.1, Lima, A. F. L.1, Duarte, S. R.1, Mello, R. J. V.2, Montenegro, L. T.2, Pontes-Filho, N. T.2 1. Faculdade Integrada Tiradentes, Brazil 2. Universidade Federal de Pernambuco, Brazil as the main prerequisite for tumor growth and metastathat secrete pro-angiogenic factors altering the structure of the vessel, making it similar to a renal glomerulus. The study of indicators of anaplasia (mitosis, hypercellularity, necrosis in palisading) in region surrounding typical and importance of the normal or changed vascular structure

and after morphological analysis selected cases that presented atypical vasculature. In each case we analyzed the mitosis parameter in the typical vascular areas to compare 40

ceptive method against STDs and unwanted pregnancies, active.Conclusion: The data in this paper on Sexual Orientation suggest that students recognize the importance of contraception and prevention against STDs and unwanted pregnancies, since all students that are sexually active do sexually active agree with sexual protection. Keywords: Sexual Orientation; STD (Sexually Transmitted Diseases); Pregnancy. CPE-1456a Biochemical parameters in animals acutely treated with Zizyphus joazeiro Mart. leaves Estevam¹ E. C.; Vilarim3, R. M.; Silva4, A. R. M.; Souza4, J. M. F. F. M. ¹Laboratório de Tecnologia Farmacêutica – Universidade Federal da Paraíba/ UFPB, Brazil. ²Departamento de Biofísica e Radiobiologia – Universidade Federal de Pernambuco/ UFPE, Brazil. 3Departamento de Biologia Molecular – Universidade Federal da Paraíba/ UFPB, Brazil.

2011 5 (2)

4 Faculdade Santa Emília de Rodat/ FASER, Paraíba, Brazil. Background: Zizyphus joazeiro Mart. popularly known for skin and stomach diseases, coughing and as a hair study was evaluate the pre-clinical toxicity of Zizyphus

agents, suggesting that the Sp-EtOHE displays gastroproactivity. -

coholic extract of the leaves of Z. joazeiro (EHAB). One of serum glucose, urea, creatinine, uric acid, cholesterol, triglycerides, ALT and AST. The results are expressed as ed male group presented a decrease in levels of uric acid

alteration was detected in the females treated with extract. high dose of EHAB administered, indicating altered liver

vonoids. CPE-1459 Clinical manifestations of the disease from the mitochondrial respiratory chain Leite, G.G.F.1,3, Araújo, V.S.1, Neves, J.W.J. S. 2,3; De Sousa, T.A.¹, Santos, E.P.¹ Estevam E. , 1,3 1. Faculdade Santa Emília de Rodat, Brazil 2. Laboratório de Percepção, Neurociências e Comportamento – LPneC/UFPB, Brazil 3. Universidade Federal da Paraíba, Brazil Introduction: The diseases from mitochondrial respiratory eases which has had a growing recognition; these diseases have constituted a group of diseases of heterogeneous gation to show the main clinical manifestations of MRCD,

could have increased the renal excretion of urate, causing a decrease in the serum uric acid. Conclusions: The made in a cautious way. Keywords: Zizyphus joazeiro leaves, toxicity. CPE-1457a Gastropotective Effect Of Sidastrum Paniculatum Fryxell (Malvaceae) Leite, T.J.A.1; Sales, I.R.P.1; Montenegro, C.A.1; Lima, G.R.M.1; Jesus, N. Z. T.1; Vanderlei, M.F.1; Batista, L.M.1 1. Laboratório de Tecnologia Farmacêutica – Universidade Federal da Paraíba, Brasil Background: Sidastrum paniculatum Fryxell popularly known as “malva-roxa” and “malvavisco”, is a tree with

which met our goals. Discussion: The MRCD are multi logical systems and the fact that it is produced mainly in the mitochondria. Being the main clinical manifestations the neurological symptoms, such as a delay in the psychomotor development, seizures, encephalopathy, myoclonus, known are the non neurological manifestations, it may cause changes in the ocular system as ophthalmoplegia and pigmentary retinopathy, the cardiovascular system shows phic cardiomyopathy, in the endocrine system is frequent liver disease and weight loss. In most cases the diagnosis

gastroprotective effect of the crude ethanolic extract (SpMRC. The treatment is guided in order to minimize the Male Wistar rats (Ratus norvegicus) (180-250 g) or Swiss mice (Mus musculus) (25-35 g) were treated with Sp-EtO-

many clinical manifestations of the MRCD due universal

induced ulcer model in rats, Sp-EtOHE (125, 250 and 500

in mice, Sp- EtOHE also showed gastroprotective effect

diagnosis. Keywords: mitochondrial, disease, multi systemic. CPE-1459a Perspectives of minor beta thalassemia from a case report De Sousa, T.A.1; Leite, G.G.F.1,3; Araújo, V.S.1; Nunes, I.A.S.1; Dias, G.A.1; Santos, G.L.2, 1. Faculdade Santa Emília de Rodat, Brazil 41

Experimental Pathology and Health Sciences

2. Universidade Federal de Campina Grande, Brazil 3. Universidade Federal da Paraíba, Brazil

common the presence of pathogens in these foods, such Keywords: Parasites, lettuce, sedimentation.

minor (heterozygous) is a genetic alteration associated with -

on a clinical case. Methodology: This case report concerns tient, female, had a microcytic hypochromic anemia, with

-

CPE-1460 In vivo studies of Poliamidoamine (PAMAM) 99mTc(CO)3-dendrimer-FITC in murine breast tumor model as a nano-carrier of anti-tumor drugs Oddone, N.1,2; Tassano, M.2; Fernández, M.2; Kramer, Benech, J.C.1 1- Instituto de Investigaciones Biológicas- Ministerio de Educación y Cultura, Uruguay; 2- Centro de Investigaciones Nucleares- Universidad de la República, Uruguay; 3- Instituto de Higiene- Universidad de la República, Uruguay Background: Poliamidoamine (PAMAM) dendrimers implanted in syngenic mice and lung metastases developed in mice with primary tumor surgically excised. Methodol-

make appropriate tests for diagnosis. If the father is hetwere done. Activity was measured at 1 and 3 h and the -

lyzing cryostat sections from liver, lung and lung metasta-

tary CPE-1459b Search helminths and protozoa in samples from lettuce (Lactuca sativa) commercialized in the city from João Pessoa - PB Leite, G.G.F.1; De Sousa, T.A.1; Araújo, V.S.1; Souza, J.P.1; Gomes, A.M.P1; Cardoso, H.M.1; Carvalho, M.M.S.G2 1. Faculdade Santa Emília de Rodat, Brazil 2.Universidade Estadual da Paraíba- UFPB, Brazil Background: The survey of helminths and protozoa in let-

parasites and other pathogenic structures in food as a matter harmful to human health. And so, food marketed to

of helminths and protozoa in samples lettuce (Lactuca sativa) commercialized in markets, greengrocers and street Samples were collected randomly in fairs, markets and Each sample was washed with distilled water in a stainless steel container, after which the water transferred to a glass of sedimentation. The sediment was analyzed in triplicate after 24 hours, with Lugol in optical microscope. -

We conclude that it is very important proper washing of

42

99mTc(CO)3-dendrimer-FITC in mammary tumor mice -

0.05, 0.3 ± 0.2. Confocal microscopy analysis of liver and h of 99mTc(CO)3-dendrimer-FITC injection, showed cytumor surgically excised. Conclusions: These results indicate that Poliamidoamine PAMAM dendrimers, are good mor and its metastases. Keywords: Poliamidoamine (PAMAM) 99mTc(CO)3dendrimer-FITC; breast tumor mice; lung metastases. CPE-1461 Kidney structural changes in mice treated with DioAnalysis Soares, P.H.S.1; Silva, L.L.S.1; Lima, C.E.Q.2; Bittencourt, A.M.3; Silva, M.G.S.1; Silva, J.F.4; Silveira, A.B.2; Almeida, E.R.5; Silva, C.V.N.S.6; Mota, D.L.1 1. Departamento de Histologia e Embriologia – Universidade Federal de Pernambuco, Brazil 2. Departamento de Ciências Farmacêuticas – Universidade Federal de Pernambuco, Brazil 3. Departamento de Anatomia – Universidade Federal de Pernambuco, Brazil

2011 5 (2)

4. Departamento de Ciências Morfológicas – Universidade de Pernambuco, Brazil 5. Departamento de Antibióticos – Universidade Federal de Pernambuco, Brazil 6. Laboratório de Imunopatologia Keizo-Asami – LIKA/Universidade Federal de Pernambuco, Brazil Introduction: The use of plants in the called “folk medicine” is a characteristic of Brazilian cultural heritage. Among the numerous plants popularly used Dioclea granis used in the treatment of renal and prostate diseases.

32 g were used, 10 animals for experimental group (ExG) and 10 for control group (CG). Both groups were maintained in an animal house with average temperature of

for seven consecutive days. The extract dosage used for -

fests in pregnancy and that interferes with the emptying ing pregnancy, normally, shows constipation. The stools and rectum causing discomfort and malaise. Conclusion: One of the indicated strategy is to monitor food given, apies, which during pregnancy can compromise the formation of the fetus. Keywords: pregnant women; gastrointestinal tract; constipation. CPE-1463a Epidemiological survey of viral hepatitis in patients with HIV in Paraíba State Benício, T.C.M¹; Menezes, R.C ¹ ; Souza, J. P.¹ ; Santos, 1.Faculdade Santa Emília de Rodat, Brazil 2.Universidade Federal da Paraíba, Brazil

procedures of ExG. Kidneys were excised, medially secB, C, D, and, F, G. Other causes include medicines, metatis has some destruction degree of the hepatic cells. In -

-

was used. Results: A marked glomerular hypertrophy was Virus (HIV) the development speed of hepatitis is faster, while in ExG the value was of 450.44 µm2. Conclusions: Results showed histological changes in renal corpuscles of

years. In this study was made an epidemiological survey of

injury to renal function. -

Complex Clementino Fraga, in João Pessoa city, Brazil, for

etry CPE-1462 Pathophysiologic changes in the gastrointestinal tract cife Farias, L.L. ; Aires, T.R.B. ; Silva, A.O.R. ; Souza, I.A. ; Anjos, F.B.R. ; Ramos, R.C.

the patients had HIV. The highest incidence in the year was in August with 58 cases and less in May, with 7 cases.

undergoes changes during pregnancy. At this stage the gastrointestinal motility decreases, even with increased

of the hepatitis epidemiology, since in the last 20 years, epidemiological and natural history studies of this disease

this study was to identify the incidence of gastrointestiin maternity of Recife. Methodology: This research was transversal, trough semi-direct questionnaires applied in Professor Bandeira Filho maternity, in the years of 2006 and 2007. The analyzed data were: family income, education level, and clinical symptoms. The women were iden-

-

diagnosis test. CPE-1464 Action Of Wine On The Liver Of Rats Submitted To DMH Administration Jorge, M.1,3 ; Duarte, R.3,4; Costa, M.3,4 ; Costa, E.1,3 ; 1- Faculdade de Farmácia da Universidade de Coimbra, Portugal. 2 -Universidade Federal de Pernambuco, Brasil. 3 -Faculdade de Medicina da Universidade de Coimbra, Serviço de Patologia Experimental, Portugal. 4- Escola Universitária Vasco da Gama, Portugal.

43

Experimental Pathology and Health Sciences

are still under investigation. In this study we evaluate liver morphologic changes associated with red wine and dimetilhidrazine consumption (DMH), a well known carcinogen. -

to the surgical intervention, and no qualitative or quantitative differences among the groups. The thicknesses of studied. Immunoexpression of PGP9.5 demonstrates in-

-

week and a complete necropsy was performed. The slides ated for morphologic lesion detection. In the group I there was not found any morphologic changes. In the group II it was found: sinusoidal congestion, fo-

and in fecal exoneration, thus restoring the evacuatory act close to the functional standard; 3) produce denervation exclusively at the site of their construction; 4) generate adaptive intestinal response with the preservation of the histological structures in the operated intestinal intervalve valves; 6) transform the intestinal segment where they are constructed into a storage segment. The technique is easily performed and has important potential for clinical application. Keywords: Biological valves, Myenteric plexus, Colorectal surgery.

nuclei with increased nucleoli.

edema and sinusoid congestion. Nuclear and cytoplasmic diffused changes, such as pyknosis, increased nuclei, macrovesicular and microvesicular steatosis, and cytoplasmic According to these results we may conclude that red wine CPE-1465 Morphology and immunohistochemistry of the myenteric plexus of valves constructed in the colon of rats subjected to abdominoperineal amputation and perineal colostomy1 Deoti, B.1*; Campos,C.1; Costa-Silva I.C.1, Hayck, J.2 Lázaro da Silva, A.3 ; Arantes, R.M.E1. 1.Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte/MG, Brasil. 2. Physician, Resident in General Surgery at the Cristiano Machado Hospital. 3. Professor Emeritus at the Federal University of Minas Gerais (UFMG), Head of the General Surgery Service at the Cristiano Machado Hospital, FUPEC / FHEMIG.

their sphincter structure and the colon pull-through via perineal colostomy. Amputated group with surgical valve (AGWV), 24 animals underwent three seromiotomy procedures on the pulled-through colon. Results: On the 40th postoperative day the intestine was processed for light microscopy (HE), morphometric and immunohistochemical analysis (PGP 9.5). The HE microscopy reveals microscopic linear interruption of muscle layers and chronic interruption of innervation at valve sites. Areas adjacent to the valves showed well-preserved structural aspect. Gomori trichrome stain showed deposits of collagen near 44

CPE-1465b Experimental Trigeminal Neuralgia: Morphological Alterations And Pain Development Oliveira, A.P.1; Licio, C.S.A.2; Siqueira, J.M.3; Massenssini, A.R.3; Moraes, M.F.D.3; Tafuri, W.L.4; Arantes, R.M.E. 4; Almeida-Leite, C.M.1 1.Departamento de Morfologia/ICB/Universidade Federal de Minas Gerais, Brasil, 2. Departamento de Ciências Biológicas, ICEB/Universidade Federal de Ouro Preto, Brasil, 3. Departamento de Fisiologia e Biofísica, ICB/Universidade Federal de Minas Gerais, Brasil, 4. Departamento de Patologia Geral, ICB/Universidade Federal de Minas Gerais, Brasil. injuries to the nervous system. Trigeminal neuralgia is a neuropathic pain localized to small areas of the face. An experimental rat model of trigeminal neuropathic pain is neuropathic pain can develop from structural impairment aim of this work was to study the morphological alterations that underlie pain development in the rat experimental model of trigeminal neuropathic pain. Methodology: group) or a sham operation without nerve ligation (Sham ated at postoperative days 3, 6, 9, 12 and 15. Trigeminal nerves were dissected and processed for optic and electronic microscopy. Results: CCI group rats showed an early hyporesponsiveness and a late hyperresponsiveness, and lesioned axons, edema in nerve fascicles, and loss of tissue organization. Demyelination and degenerated mypain states in humans and experimental models, were also and detachment of myelin. Morphometric analysis showed greater celularity in CCI group nerves, which can occur as a result of glia proliferative response to nerve lesions. vealed severe structural impairment of nerve fascicles and

2011 5 (2)

trigeminal neuropathic pain development. KEYWORDS: neuropathic pain, trigeminal nerve, demyelination.

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APQ-00489-09) CPE-1465c Aspects Of Interaction In Vitro Between Microgliocytes And Leishmania Major Souza, C.C.1, Costa-Silva, I.C.1., Campos, C.F.1 , Cangussú, S.D.2,; Almeida-Leite, C.M3, Vieira, L.Q.4, Arantes, R.M.E1 1- Departamento de Patologia Geral, ICB - UFMG - Belo Horizonte/MG, Brasil. 2- Departamento de Ciências Biológicas, ICEB - UFOP - Ouro Preto/MG, Brasil. 3- Departamento de Morfologia, ICB - UFMG - Belo Horizonte/ MG, Brasil. 4- Departamento de Bioquímica e Imunologia, ICB – UFMG, Brasil. Background: Microgliocytes (MG) are central nervous system cells that share origin and function of macrophages odology: We investigated the in vitro interaction of MG of

rosy were selected, all of them were in treatment for less than 30 days and presented at least one cutaneous lesion. sensory analyzer was used. For morphological analysis, -

Package for Social Sciences (SPSS) 15.0 software. Results: The imunohistochemical staining showed decrease of in-

density was intense and homogeneous. Conclusions: The skin lesions is an investigative and important tool to understand the mechanisms of disease that involve peripheral -

culture for trials interaction of one hour (adhesion) and 48 h (infection) with L. Major. The participation of CR3 Financial Support: CNPq and FAPEMIG grants anti-CR3. Results: MO shows higher rate of adhesion than was higher. Addition of SD increased adhesion parameters

show different characteristic regarding the interaction with tion of SD. Addition of SD and anti-CR3 showed the direct participation of CR3 in the interaction. However, as it a participation of an alternative interaction and internalizaKeywords: Microgliocytes, central nervous system, macrophages. CPE-1465d Correlation Between Density Of Nerve Fibers In Skin Biopsy And Pattern Of Termic Sensibility In Patient Lesions With Leprosy Lyon, S.2, Villarroel, M.F.3, Arantes, R.M.E.1 1Departamento de Patologia Geral, UFMG, Belo Horizonte/ MG, Brasil 2Fundação Hospitalar do Estado de Minas Gerais, Belo Horizonte/MG, Brasil 3Departamento de Medicina Tropical, UFMG, Belo Horizonte/ MG, Brasil

CPE-1465e lated To Variation In Nociceptive Thresholds Of Mice Ear Leishmania Major-Induced Lesions Cangussú S.D.1; Souza CC2; Campos C.F.2 Vieira L.Q3; Afonso L.C.C1; Cunha FQ4; Arantes R.M.E. 2 1Departamento de Ciências Biológicas, ICEB, UFOP, Ouro Preto, MG, Brasil 2Departamento de Patologia Geral, ICB, UFMG, Belo Horizonte, MG, Brasil 3Departmento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brasil 4Faculdade de Medicina de Ribeirão Preto, USP, São Paulo, Brasil healing lesions and Th1 response. Th1 and Th2 cells seel, we investigated variation in nociception along 12 weeks (wk) post infection and its relationship with structure of morphological analysis indicate variation in the aspects of nociception in wk 2, related to increased levels of IL-6, in ear. Exogenous IL-10 was demonstrated to prevent the hypernociception in this infection model. In support to

The evolution of disease cause severe damage and perma-

endogenous IL-10 in hypernociception. Moreover, in this 45

Experimental Pathology and Health Sciences

(SRE) in the spleen and liver. The erythrocytes decrease the survival time of these cells, through their removal from -

-

the mechanisms of hyponociception may envolve downregulation of cytokines, preservation of nerve endings structure, differences in neurotransmitter release or some

in patients with self immune diseases. The evolution is ex-

MIG grants KEYWORDS: cytokines, nociception, L. major infection

taneously, after months or years of evolution. The incicommon in males, however, is often in females during the

CPE-1466 implants in Ehrlich tumor-bearing mice Da Silva, G.R.1; Pereira, A.F.1; Costa, V.M.1; Santos, M.C.M.1; Pinto, F.C.H.1 1. School of Pharmacy, Federal University of São João Del Rei, Divinópolis, Brazil

AHAI remain unknown. Some hypotheses are: the depresalteration among the T cells facilitators and suppressors, changes of the antigen from surface of the erythrocytes

release delivery systems of therapeutic agents incorporated that, it is so important, that the professional from the to provide the patient quality assistance. mice was investigated. Methodology: Implants were preimplants without MTX were also produced. In vivo release taneous tissue of mice. A total of 20 Swiss female mice, two months old were divided into two groups of 10 anicells (0.05 mL) on the footpad. At 5 days post-inoculation, the animals of the A and B groups received PCL implants paw thickness, using a digital pachymeter. At 12 days post-

CPE-1468 Current understanding of the multi form glioblastoma (GBM) and new therapeutical potentials Santos, A.H.1; Pereira, B.D.S.1; Silva, S.R.1; Cardoso, H.M.1; Gomes, A.M.P.1,2; Nunes, M. K. S 1,2; Silva, B.D.F.1; Machado, W.G.S.1; Santos, E.P.1; Souza, J. P1,2; 1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil 2. Federal University of Paraiba, student of Technical Biodiagnostic, Brazil. 3. Biological Science Center, State University of Paraiba, Brazil 4. Oral Biology Laboratory, Health Science Center, Federal University of Paraiba, Brazil astrocytic tumor with higher degree of malignancy, cor-

cutaneous tissue of mice. Animals of the B group showed

of the World Health Organization (WHO). It is a primary tumor from the central nervous system more common in

drastic decrease of cellularity in the B group. Conclusion:

Related To Variation In Nociceptive Thresholds Of Mice Ear Leishmania Major-Induced Lesions high capacity of

and polymorphic cells. Its growth is often in the fronKeywords: methotrexate-loaded poly(caprolactone) implants; Ehrlich tumor; local drug delivery system. CPE-1467a Clinical and therapeutical aspects of the self immune hemolytic anemia (AHAI) Benício, T.C.M ¹ ; Santos, E.P ¹ ; Diniz, D.A.M¹ ; Souza,

vide a current understanding of GBM and discuss the new future therapeutical target potentials. Methodology: Through a systematic known literature review, as well as a critical analysis and comparative of works, articles from start to elucidate a multi form network of genetic changes

1.Faculdade Santa Emília de Rodat, Brazil 2. Universidade Federal da Paraíba, Brazil The self immune hemolytic anemia is a disorder from idiopathic source or secondary to other self immune diseases -

46

migration and invasion, opening the door to new discovershown that genetic loss, due changes in the genomic level, erozygosis of the chromosome 10, the most frequent al-

2011 5 (2)

-

3.Santa Emilia de Rodat Faculty, Brazil Introducion: Falciform anemia is a genetic disease of

(EGFR) in chromosome 7. Perhaps the greater mystery in the comprehension of the oncogenomic of GBM is to decide how and when these genetic changes and the signaling cascade interact. Conclusion: Clearly, many of these There is a real hope for patients who suffer with this illness in the future. Keywords: multi form glioblastoma; astrocytic tumor. CPE-1469b Hereditary Hemochromatosis: “Rust in the body”

-

of the molecule changing its charge and electrophoretic cidate the importance of genetic counseling in hereditary occurrence of sickle cell anemia, for individuals previously the gene, are assisted and know the risks of recurrence for -

1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil 2. Molecular Biology laboratory, Santa Emilia de Rodat Faculty, Brazil 3. Master of Bioactive Natural Products and Synthetic, State University of Paraiba, Brazil 40 to 160 micrograms, there are those who accumulate in large quantity than necessary, carriers with hereditary hemochromatosis (primary), an autosomal recessive disease arm of chromosome 6, which secretes a protein trasmem-

tients with anemia or sickle cell trait. Results: According to the Ministry of Health estimates that there are more clinic complications and other relatively common. Overall ity to infections. The little foot exam is used for detecgene therapies are limited. Conclusion: Genetic counseling

mochromatosis through a literature review at documents less frequent S65C. Results: The mutation from most clinically relevant is it G -> A at nucleotide 845, resulting (C282Y) and decreasing the density of the protein HFE dine to aspartic acid (H63D) compromises the interaction position 65, due to the transversion of adenine nucleotide A -> T, at position 193 of the HFE gene. Conclusion: The mutations genetic screening allows the knowledge and awareness of genetic risk of disease development, and early diagnosis of individuals at an early stage. There is

sickle cell disease or trait, requiring implementation of a program of early diagnosis and genetic orientation, psychological and social support for these individuals. Keywords: genetic counseling, sickle cell anemia, public health. CPE-1482 Morphometric changes in hearts of rats under malnutrition and chronic exercise during the period of lactation Alves, R. M. R.¹; Guendler, J. A.¹; Pontes Filho, N. T.¹; Melo Júnior, M. R.¹; Araújo Filho, J. L .S.¹ ; França, T. J. B. 1.Department of Pathology, Federal University of Pernambuco (UFPE), Brazil 2.Department of Anatomy , Federal University of Pernambuco (UFPE), Brazil Background: Exercise and undernutrition can cause different effects in heart structure and function. However hearts morphometric changes are not totally explained, mainly in developmental period, when some damages induce the ate the morphometric changes in heart of young malnour-

iron, where accumulates in the liver, pancreas and heart, can lead to death from cirrhosis, hepatocellular carcinoma,

males Wistar rats in lactation period were divided in four

Keywords: Iron, Mutation, Hemochromatosis

exercise (NE); undernourished control (DC) and under-

CPE-1469c The importance of genetic counseling in sickle cell anemia Leal, E. B. N.¹; Rodrigues, M. L.¹; Martins, L.L ¹ Estevam,

gressive program of daily sessions of free swimming carried through 8th to 23rd days of life. After, infusion was

1. Molecular Biology laboratory, Santa Emilia de Rodat Faculty, Brazil 2. Master of Bioactive Natural Products and Synthetic, State University of Paraiba, Brazil 47

Experimental Pathology and Health Sciences

in the nourished than in malnourished, no difference rewas higher for NC and NE than for DC and DE groups.

Brazil 2-Departamento de Fisioterapia – CCS/UFPE, Brazil 3-Departamento de Patologia – CCS/UFPE, Brazil Background: Exercise and undernutrition can cause differ-

higher than the NC group. There were no differences in cleus were higher in nourished groups than malnourished and in NE than NC. Conclusions: Protein undernutrition associated with exercise during the lactation period did not gly, exercise and undernutrition caused changes in heart sults. We concluded that protein malnutrition leads to a CPE-1482a Analysis of neurons NADPH-diaphorase in the cerebral cortex of animals exposed to perinatal malnutrition and alcoholism 1. Institute of Integrative Medicine - Professor Fernando Figueira/ IMIP, Brazil, 2. Department of Pathology, Federal University of Pernambuco (UFPE), Brazil Background: The various effects of ethanol and malnutrition to the Central Nervous System such as microcephaly, mental retardation, motor dysfunction and cognitive impairment are well understood and widely recognized. However, the mechanisms that induce these changes still reason, the present study evaluated, through morphometric parameters, the effects of malnutrition and ethanol over length of the primary extensions of Neurons NADPHdiaphorase and the neuronal density in cortical layers of zone of 40 day-old Wistar male rats, generated and sucktein) and alcohol administration (commercial aguardente administered treatment (Nutrition (N), Nutrition Brandy (NB), Nutrition Ethanol (NE), Malnutrition (M), Malnutrition Brandy (MB), and Malnutrition Ethanol (ME). The -

morphometric changes are not totally explained, mainly in developmental period, when some damages induce the ogy: 42 males Wistar rats were tested during the lactation period. The animals were exposed two different nutritional with swimming: well-nourished control (NC); well-nour-

of daily sessions of free swimming carried through of 8th to 23rd day of life and they swam during 15, 20, 25, 30 and ment the somatic development parameters were evaluated: corporal weight, latero-lateral axis of skull, length of tail metrical analysis evaluated the cellular area and density of the neurons of motor cortex somatic development. Conclusions: Our results indicate that the proposed swimming parameters evaluated, as well as it did not interfere in the of the neuronal cellular area. Keywords: protein malnutrition, exercise and brain CPE-1483 Plants of the genus Bauhinia inhibit the activity of gelatinase B R.I.M.A1. 1 Universidade Federal de São João del-Rei - Campus Centro-Oeste Dona Lindu, Brazil. Background: The matrix metalloproteinases (MMPs) are endopeptidases with degrade components of the matrix extracellular in physiologic e pathologic process. MMP-9 related to studies involving tumor progression and metastasis. Plants of the genus Bauhinia, known as Pata-de-vaca tives: To assess the activity of crude extracts, hexane, leaf

C) of the genus Bauhinia, on the proteolytic activity of geneuronal density as well as the NADPH-diaphoreses neu-

of Bauhinia: 1A, 1B, 1C, 2A, 2B, 2C, 3A, 3B, 3C. Areas of and densitometry was performed using Scion Image. Phytochemical tests to detect the presence of secondary

during the perinatal development. Keywords: ethanol, malnutrition, cerebral cortex CPE-1482b Morphometric changes in hearts of rats under malnutrition and chronic exercise during the period of lactation Guendler, J. A. 1; Alves, R. M. R.1; Melo Júnior, M. R. 1; Araújo Filho, J. L .S. 1 ; França, T. J. B. M 1; Ferraz, K. M. 2; Pontes Filho, N. T. 1-3; Costa, M. L. C. R.3 1-Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, 48

statistical analysis, we found that all of Bauhinia extracts

C2, C3, B2, A2, B1, respectively. The phytochemical study of extracts was positive for steroids, triterpenoids, couma-

2011 5 (2)

-

Keywords: Gelatinase B, Bauhinia, Zymogram. CPE-1484 Evaluation of the immunomodulatory effect of monoterpenes in experimental arthritis Brasileiro-Silva, L.A.¹; Ramalho, T.R.¹; Moreira, M.M.B.2; Castello-Branco, M.V.S.2; De Sousa, P.D3; Piuvezam, M.R.1; Bezerra-Santos, C.R.1 1. Laboratory of Imunopharmacology, Federal University Paraíba, Brazil 2. Laboratory of Toxicology, Federal University of Paraíba, Brazil 3. Department of Physiology, Federal University of Sergipe, Brazil Background: Rheumatoid arthritis (RA) is a systemic au-

buco (UFPE), Brasil 2. Departamento de Bioquímica - UFPE, Brasil 3. Departamento de Física - UFPE, Brasil 4. Laboratório de Pesquisas em Virologia - Faculdade de Medicina de São José do Rio Preto (FAMERP), Brasil

posites (NcAuPANi) associated to Cratylia mollis lectin (CramoLL) for detection of dengue serotypes 1 and 2. -

were used as counter electrode and reference electrode, respectively. The electrochemical impedance spectrosare well known. Natural products therapies using mono-

for the characterization and interfacial monitoring at dif-

study was to analyze the potential immunomodulatory efmonoterpenes were tested for their toxicity using Artemia -

owing to the adsorption of NcAuPANi–CramoLL, and dengue serum. It demonstrated that CramoLL retained

opment the delayed-type hypersensitivity reaction (DTH) edema was performed in different periods of time after challenge procedures. Results: There was no toxic effect After OVA-sensitization and -challenge, mice developed a ed only with soil oil (vehicle). Oral treatment (p.o) with 3.78 ± 0.36, respectively) in comparison with non-treated (3.73 ± 0.74; 4.02 ± 0.8, respectively) also did not alter paw edema formation in comparison with non-treated mice (3.88 ±0.62)). In a separate experiment, mice were treated for 28 days after challenge and also did not reduce paw edema formation. Similarly, dexamethasone (p.o.) also did not reduce paw edema formation indicating that after inmation status unless used at long period of time. Concluthat these monoterpenes might present protective effects. Keywords: Monoterpenes, arthritis, mice

recognize glycoproteins present in dengue serotypes 1 and Keywords: biosensor; gold nanoparticles; lectins. CPE-1487 Masseter evaluation in experimental intoxication with Tioacetamide Joseíde Félix1,2, Joana Costa3, Felipe Pereira4, Rodrigo Farinha2, Maria Emilia Ramos5 1 Graduating in Dentistry- State University of Feira Santana 2 Experimental Pathology Service, Faculty of Medicine, University of Coimbra 3 Chemistry Department, Faculty of Sciences and Technology, University of Coimbra 4 Faculty of Pharmacy, University of Coimbra 5 Department of Health, State University of Feira de Santana, Feira de Santana, BA, Brazil or a consequence of the symptoms linked to temporotoxic is a source of sulfur in the industry, used for organic production, as well as in metallurgy and for pesticide synthesis. Tiocetamide, is an experimental inductor of hepatic disease in the rat, producing lesions from necrosis to hepatic carcinoma, depending on time and dose. The aim of cetamide administration on the masseter muscle. Twenty

CPE-1486 A new approach in the development of dengue biosensor by using hybrid nanocomposites and Cratylia mollis lectin

of the 4th week, and fragments of masseter muscle were collected for routine histopathological analysis, and frozen

1. Centro Acadêmico de Vitória - Universidade Federal de Pernam-

studies. Ten pictures were taken of each slide of masseter 49

Experimental Pathology and Health Sciences

ric analysis was performed with software ImageJ, for the

The analysis of the results of the morphologic analysis of masseter muscle on tiocetamide administration, shown no

Pediátrica – HUOC - BRAZIL. Background: SLE is an autoimmune multifactorial disease TNF may play an important role in the development and clinical manifestation. TNF promoter polymorphism has -

CPE-1488 Toxicological evaluation in mice with extract of Phyllanthus tenellus Roxb Silva, T.C.L.1; Santos, L.S.1; Ferraz, I.C.1; Silveira, M.M.B.M.1; Lorena Júnior, E.J.P. 1; Veras Filho, J.2; Higino, J.S.2; Araújo, E.C.1 ; Souza, I.A.2 ; Amorim, E.L.C.2 1. Dep. de Enfermagem - Universidade Federal de Pernambuco (CAV) – Brasil 2. Dep. de Farmácia - Universidade Federal de Pernambuco – Brasil

ology: We realized a case–control study contein 59 lupus cases and 76 lupus-free adults controls. Diagnosis of SLE Rheumatism Association criteria (ARA), and disease activat the Rheumatology Clinic of the Hospital das Clínicas and the differences were analyzed using the ”Odds Ratio” and “G” test, respectively; a p value of less than 0.05 was

calculated using the software BioEstat 5.0. Results: When -

groups, the results showed that the frequency of A allele

Methodology: We performed the second testing method repeated exposure of mice within 45 days evaluating the mice with daily for signs of toxicity. Results: The shortas nontoxic. However the species showed adverse effects

we analyzed whether genotypes are associated with clinical -

a depressant effects including: drowsiness, prostration, dehowever the group that received the higher dose experi-

CPE-1489a

respiratory rate, prostration, piloerection, swollen muzzle,

Crohn Disease

plant for use depending on its concentration. Conclusions: “natural” are safe is frequent, thinking that they present no risk of toxicity, can lead to serious consequences, side effects, interactions and poisonings unprecedented, making it necessary measures of education and effective informaplants and their derivatives. Keywords: P. tenellus, LD50, subchronic. CPE-1489 Polymorphism in region promoter of Tumor Necro(SLE) susceptibility Angelo, H.D.S.1,2; Fernandes, W.N.3; Silva, H.A 4; Asano, N.M.J 5; Lins, O.G.6; Maia, M.M.D1; Muniz, M.T.C2,7; Souza, P.R.E1,2. 1 - Laboratório de Genética-Bioquímica e Sequenciamento de DNA - UFRPE - BRAZIL; 2 –Instituto de Ciências Biológicas - UPE - BRAZIL; 3 – Programa de Pós-graduação em Patologia - UFPE - BRAZIL; 4 - Departamento de Genética – UFPE - BRAZIL 5 - Departamento de Medicina Clínica da UFPE - BRAZIL; 6 – Departamento de Neuropsiquiatria da UFPE – BRAZIL; 7 - Laboratório de Biologia Molecular, Centro de Oncohematologia 50

gelo, H.D.S.3,4 , Souza, P.R.E3,4 , Torres, D.O.C.5, Martinelli, V. 6 , Lima, F.E.6 ,Telles, A. M. S.7 , Melo-Junior, M. R.7,8. 1- Programa de Pós-graduação em Patologia - UFPE - Brazil 2- Centro de Ciências da Saúde – UFPE- Brazil 3- Laboratório de Genética-Bioquímica e Sequenciamento de DNA – UFRPE – Brazil 4- Instituto de Ciências Biológicas - UPE - Brazil 5- Pronto Socorro Cardiológico de Pernambuco – UPE-Brazil 6- Hospital das Clínicas – UFPE- Brazil 7- Departamento de Patologia- UFPE-Brazil 8- Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, Brazil and disease severity, prognosis and monitoring the outcome of therapy and avoid invasive procedures. late these measurements with the anatomical and clinical forms of the disease. July 2011, 49 patient outpatient services of Gastroenter-

2011 5 (2)

disease from clinical, radiological, endoscopic and histo-

man kala-azar among patients undergoing serological test -

Carlos Diniz located in the city of Patos - PB. Material and Methods: This is a documentary study, retrospective with quantitative approach in which records of 802 patients with suspected human leishmaniasis from various regions

-

in light of the literature concerning the matter. Throughout the study, the secrecy of the sample was preserved, according to Resolution No. 196-96, which regulates rethrough the records was: origin of patients, age, sex and results of serological tests. Results: It was found that out

from these exams.

clinical evolution and the chances of reicidiva. Keywords: Crohn disease; ultra sensitive C-reactive protein(US-CRP); erythrocyte sedimentation rate(ESR). CPE-1490 Serological surrvey of antibodies IgG anti-Toxoplasma gondii in cattle from Zona da Mata of Pernambuco state, Brazil Guerra, N. R.1; Alves, B. H. L. S.1; Monteiro M. F. M.1; Sandes, H. M. M.1; Santana, V. L. A.2; Souza, M. M. A.3; Santos, A. S.1; Silva C. M. S. L.1; Mota, R. A.1; Alves, L. C.1 1. Universidade Federal Rural de Pernambuco, Recife, Brasil 2. Fiscal Federal Agropecuária- MAPA 3. Fiscal de Defesa Agropecuária- ADAGRO Background: Toxoplasmosis is a zoonotic infection trans-

erly, has high rates of mortality. Thus, it is concluded that these results are relevant to the disease frequency in the study area, as well as comparative studies carried out later. Keywords: Leishmania - disease – Paraiba CPE-1519 Bioactivity of extracts obtained from aerial parts of Croton grewioides Fernandes, H. M.B.1; Pessoa, D.R. 1; Viana, W.P. 1; Medeiros, V.M. 1; Oliveira Filho, A.A1; Fernandes, H.B. 1; Tavares, J. F. 1; Silva, M. S. 1; Diniz, M. F. F. M. 1; Cas1. Laboratory of Pharmaceutical Technology, Federal University of Paraíba- Brazil Introduction: The genus Croton is one of the largest of

goondi oocysts in cat feces, and also through consumption of raw or undercooked meat with cysts and drinking unpasteurized milk. However, cattle are less suscep-

-

IgG anti-Toxoplasma gondii in cattle from Zona da Mata from 427 cattle herds located in 13 counties from Zona

evaluated the toxicity of hexane extract, chloroform, ethyl acetate and ethanol of Croton grewioides through the

sults showed the Chã de Alegria County with highest counties and the contamination of food and water with T. goondi oocysts from cat feces are discussed. Keywords: Toxoplasma gondii, Serological, Cattle. CPE-1507 Frequency Of Human Kala-Azar In The Interior Of Paraíba

for 24 h and then the survivors larvae were counted to delications were done for each concentration and the experishrimp test for hexane extract, chloroform, ethyl acetate -

Faculdades Integradas De Patos, Patos – Pb sites of the genus Leishmania. In the vector, the parasites on extracts of hexanico, acetate de etila and cloroformico, intense and widespread parasitism of cells of monocytic macrophage system, with massive hepatosplenomegaly. 51

Experimental Pathology and Health Sciences

Phytochemical studies with them are underway to isolate Keywords: Croton grewioides, Artemia salina, Bioactivity.

of the enzyme cyclooxygenase) or tempol (superoxide dismutase mimetic). Results: The HFD-group had a higher

CPE-1519a Wanderley University Hospital Fernandes, H.M.B.1, Oliveira Filho, A.A.1, Fernandes, H.B.2, Oliveira, T.L.1 1 - Health Sciences Center, Federal University of Paraíba - Brazil 2 - Biomedicine Course, Integrated Faculties of Patos - Brazil versity Hospital Lauro Wanderley. Material and Methods: This study is an epidemiological survey, developed from Hospital Lauro Wanderley. Records were used, containing results of parasitological examinations of 7844 patients, internal or who sought outpatient care. The survey was conducted from tests made in the period January to Despecies of parasites, as well as their frequency. Results: -

test). In the vascular reactivity evaluation, the HFD group

ute to endothelial dysfunction in high-fat diet-induced Keywords: Obesity; Endothelial Dysfunction; Metabolic Syndrome. CPE-1520a Association between diabetes mellitus and nephropathy: A retrospective study of patients treated at the University Hospital of Recife 1Silva, O. A.; 1Martins, J.C.M.; 1Silva, J.J.T.; 2Silva, M.R. 1. Departamento de Enfermagem da Universidade Federal de Pernambuco 2. Departamento de Enfermagem da Universidade de Pernambuco leading cause of chronic renal failure (CRF) in various greater insight into the association of these two patholo-

contained parasites. Among the positive results, helminths to clinical impairment of patients with CRF in Pernamventions, on the means of contamination, prevention and Keywords: Intestinal parasite, Helminths, University Hospital Lauro Wanderley CPE-1520 Tempol or indomethacin improves vascular dysfunction in obese rats 1Silva, O. A.; 1Queiroz, D. B.; 2Souza, V. N.; 2Fonseca, L. A.; 1Duarte, G.P. 1. Departamento de Fisiologia e Farmacologia da Universidade Federal de Pernambuco, 2. Departamento de Fisiologia e Farmacologia da Universidade Federal de Alagoas medical disorders that, when occurring together, increase

researched the medical records of 98 patients with CRF were divided into two groups: NDM Group, patients with diagnosis of CRF and MD Group, patients with diagnosis of CRF and DM. The data analyzed were related to epidemiological and clinical characteristics of the patients. the NDM group and 40 in DM group. In this, there was a mean age higher than that in the NDM group (60 vs. 51 years, respectively). The mortality rate was higher in DM). In the latter, the evolution to dialysis was faster. The

had lower urine output. Conclusions: It is shown that the

environmental factors, especially the consumption of distudy was to evaluate the effect of high fat diet (HFD) on vascular reactivity of young rats. Methodology: Eightweek-old male Wistar rats were divided into two groups assigned to receive, with free access, two kinds of diet: a

entire period of dietary intervention. At the end of the diet period (6 weeks), we evaluate the vascular relaxation 52

action on health and therapeutic adaptation to this type of patient. Keywords: Chronic Renal Failure, Diabetes mellitus, Morbidity, Mortality. CPE-15 Epidemiological Study Of People With Acute Barber Bug Fever On Northern Of Brazil Gomes, A. C. L.1; Da Silva, P. K. F. 1; Silva, P. K. F. 1; Soares, L. H. S. 1; França, M. J. D. M. 1; Costa, B. G. S. 1; Lorena Júnior , E. J. P. 1; Silva, K. D. M. 1; Melo, M. S. 1;

2011 5 (2)

Patriota ,H. S. 1; Melo, G. P. 1 1. Universidade Federal De Pernambuco- Ufpe/Cav -Brazil

Patriota ,H. S. 1; Da Silva, P. K. F. 1; Silva, P. K. F. 1 Moura, T. M. L. 1; Gomes, C. C. S. 1 ; Oliveira, R. A. A. 1; Bar1. Universidade Federal De Pernambuco- Ufpe/Cav -Brazil

cruzi, from the “sting” of triatomines, known in Brazil as immunity individuals or evolve to a chronic form. With -

-

premature realization of elective cholecystectomy laparoscopic. Even with the advance in laparoscopic procedure, the patients are not risk free of show post discharge altoms of a patient with choledocholithiasis, systematize and American Nursing Diagnosis Association). Methodology: It is a descriptive study of the kind case report, done in a

controlling BBF actions on the last years, emerging the need of rethink the used strategies in the disease control, adoptions of integrative politics and effective actions of epidemiological vigilance with special attention to the major risk group cited in this research. Keywords: Barber bug fever, Epidemiological, Northern Region.

to 15th of July of 2011, where a patient with secondary choledocholithiasis was accompanied to iatrogenic stenolecystitis. Results: During the period of data collect, were track the diagnostics according to the patient general state needs; (3) acute pain; (4) impaired tissue integrity; (5) risk

CPE-1522 Prenatal Care: A Tool For Early Detection Of Hepatitis B Virus In Pregnant Women Santos, LS1; Moreira, LR1; Souza, GML1; Souza, VP1; Bezerra, MRE1; Santos, RP1. 1. Universidade Federal de Pernambuco, Brasil.

portance the nursing assistance and systematization has

a virus directly cytopathic, causing different clinical responses that can vary since no of symptoms to fulminant hepatitis, chronic hepatitis, cirrhosis and hepatocellular carcinoma featuring the pregnant women that are a high risk group to transmission of this disease, the need for an -

CPE-1524 Pharmacognostic study and preliminary toxicological Pseudobombax marginatum (St.-Hil.) Robyns Veras Filho, J.1; Basto, S.R.L.2; Ferraz, I.C.3, Peixoto Sono, J.S.1; Silva, T.C.L.3 1. Dep. de Farmácia - Universidade Federal de Pernambuco – Brasil 2. Dep. de Biologia - Universidade Federal de Pernambuco (CAV) – Brasil 3. Dep. de Enfermagem - Universidade Federal de Pernambuco (CAV) – Brasil Background: Studies ethnopharmacological on Pseu-

knowledge and attitude of pregnant women in the city of B in pre-natal care. Methods: This study was carried out

to virus research. Conclusions: The implementation of educational practices to prenatal care on the transmission vention and early detection, controlling the spread of the disease and reducing mortality. keywords: Gestantes; Hepatite B; Cuidado Pré-natal CPE-1523 Secondary Choledocholithiasis To Stenosis Iatrogenic Of Biliary Ways: Understand The Pathology And Implementing The Nursing Assistance

necessary the general knowledge of the disease, keeping in Keywords: cholecystitis; cholecystectomy laparoscopic; choledocholithiasis.

to analyze the antioxidant activity and preliminary assess the toxicity against the larvae of Artemia salina Leach. Methods: Chromatographic methods were used according to Wagner and Bladt (1996) is aimed to highlight the methods of Folin-Ciocalteu (1927) to determine the phe-

53

Experimental Pathology and Health Sciences

anthocyanins, aglycones and glycosidic anthraquinones, phenolic acids), coumarins and terpenoids. The groups that stood out were the diversity of terpenoids and phe-

CPE-1526 Preliminary screening of biological and toxicological Ziziphus joazeiro Mart ;

1

cies. With regard to antioxidant activity, the sample showed

1. Dep. de Enfermagem - Universidade Federal de Pernambuco (CAV) – Brasil 2. Dep. de Farmácia - Universidade Federal de Pernambuco – Brasil Background: Ziziphus joazeiro Mart. popularly known their green leaves, even in periods of drought. The high content of saponins found, the species provides a deterfor cleaning teeth, skin whitening and skin diseases. It

and antioxidant. Keywords: P. marginatum, phytochemicals, Artemia salina. CPE-1525a Basic Techniques Of First Aid In The School Environment: A Short Course Given To County Teachers In The City Vitória De Santo Antão Galindo Neto, NM¹; Oliveira, RAA1; Muniz, MLC1, Santos I.T.S1, Costa, AB1, Santos ECB1, Silva ACA2, Santos LRC2 1, Nursing Department. Federal University of Pernambuco, Vitória Academic Center, Brazil. 2.Faculdade de Enfermagem Nossa Senhora das Graças-FENSG Universidade de Pernambuco- UPE Background: The school environment is prone to various kinds of accidents and the very nature of school age favors such occurrences. In emergency situations, the evaluation

ing preliminary test of toxicity. Methodology: To evaluNCCLS standards. We used strains of Gram-positive and

-

-

professionals to have access to the injured pupil, so the knowledge of right course of action on the part of such professionals has great relevance for the successful initial care, while assistance does not arrive or so that appropriaid techniques taught in the school environment for teach-

Keywords: Z. joazeiro, antimicrobial, Artemia salina.

which occurred during the Regional Meeting of SBPC in CPE-1531 Importance of vaccines against Human papillomavirus – HPV theoretical and practical approaches. The facilitators were teachers and students of the undergraduate nursing course as participants. We used audiovisual resources, data, mannequin cardiopulmonary resuscitation for simulation and tory, the teachers demonstrated that they had learnt the theoretical content and also gave satisfactory performances in hands-on sessions. Conclusions: Trainings for teachoptimize the chance of life and the reduction of sequelaes support during the training of education. Keywords: Health education, accidents in the school environment, teacher training 54

A.P.B.4; Tavares, I.F.5; Melo, L.N.T.6 1. Departamento de Ciências Farmacêuticas – UFPE/ Brazil 2. Departamento de Ciências Biológicas - UFPE/ Brazil 3. Departamento de Enfermagem - UFPE/ Brazil 4. Faculdade Integrada da Vitória de Santo Antão- FAINTVISA/ Brazil 5. Departamento de Ciências Biológicas, Universidade de Pernambuco – UPE/ Brazil 6. Departamento de Ciências Ambientais – UFPE/ Brazil the family Papillomaviridae, is a DNA virus from sexual transmission that is highly prevalent in man and woman and cause anogenital warts and cancer. Sexual contact is er ways such as the fetal-maternal, self-inoculation from -

2011 5 (2)

jectives: The aim of this study was to perform a survey of and the importance of vaccines against human papillomavirus - HPV in this context. Methodology: For that, updatprojects that address this issue. Results: Currently we have from VLPs derived from a single viral protein, L1, which is a major structural protein of the virus, which contains its immunodominant epitope. These vaccines protect against

so that the immune system recognizes it and avoids a viral vented from installing themself. Conclusions: According

clusion: The results demonstrate the need of an epidemiological control and greater vigilance to reduce the impact health. Keywords: Hepatitis B. Epidemiology. Public Health. CPE-1532a Epidemiological Situation Of Schistosomiasis Manson In Brazil During The Period 2008 To 2011 Costa, B.G.S.¹; Lorena Júnior, E.P.¹; Melo, M.S.¹; Silva, 1. Departamento de Enfermagem - UFPE/ Brazil 2. Departamento de Ciências Biológicas – UFPE/ Brazil 3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil 4. Faculdades Integradas da Vitória de Santo Antão - FAINTVISA/Brazil 5. Departamento de Enfermagem – Universidade de Pernambuco, UPE/ Brazil Background: Schistosomiasis is an infectious disease

in young patients associated with an effective screening family, gender Shistosoma. This represents an endemtoday. Keywords: Human Papillomavirus. Vaccines. Cervical carcinomas

transmission occurs through the long human with water containing cercariae and clinical outcome may vary from zil. Methodology: For this, a survey was conducted in the

CPE-1531a Analysis Of The Occurrence Of Hepatitis B In Pernamcountry in the period 2008 to 2011, taking into considerCosta, B.G.S.¹; Lorena Júnior, E.P.¹; Melo, M.S.¹; Silva, area of residence and evolution). Results: During the study 1. Departamento de Enfermagem - UFPE/ Brazil 2. Departamento de Ciências Biológicas – UFPE/ Brazil 3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil 4. Faculdade Integrada da Vitória de Santo Antão - FAINTVISA/ Brazil 5. Departamento de Ciências Biológicas - Universidade de Pernambuco/ Brazil 6. Departamento de Ciências Ambientais - UFPE/ Brazil 7. Departamento de Enfermagem – Universidade de Pernambuco/ Brazil

sion: The results show the necessity of a program aimed at the epidemiological control of disease, since it is an important endemic parasitic. Keywords: Schistosomiasis. Epidemiology. Shistosoma mansoni.

padnaviridae, genus Hepadnavirus. It represents a major

CPE-1533 Epidemiological Situation Of Yellow Fever In Brazil During The Period From 2007 To 2010

-

-

cases of viral hepatitis has increased in recent years causing great expense to care for infected patients. According to the World Health Organization (WHO), more than two

1. Departamento de Ciências Biológicas – UFPE/ Brazil 2. Departamento de Enfermagem - UFPE/ Brazil 3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil 4. Faculdade Integrada da Vitória de Santo Antão -FAINTVISA/Brazil 5. Departamento de Ciências Biológicas- Universidade de Pernambuco – UPE/ Brazil

ducted a survey at the Sistema de InfoNational Informa-

Flaviviridae, genus Flavivirus that infects the cells of our in the period 2008 to 2011, taking into consideration the of residence and clinical forms of the disease). Results: During the period of the study, 3497 cases were reported, 55

Experimental Pathology and Health Sciences

of yellow fever cases reported in Brazil. Methodology: We data collection, refering to the yellow fever cases reported during the period from 2007 to 2010, examining some education, evolution, and year). Results: During this peserved that the affected individuals are usually young men, carrying out agricultural activities, extractive, practitioners of eco-tourism areas of risk where step into forest areas without vaccination. Conclusions: Thus, there is a need to make an expansion of coverage, especially for those individuals most affected, such as strengthening of vector control actions in the municipalities located near areas of transmission. Keywords: Yellow fever. Aedes aegypti. Haemagogus. CPE-1533a

that integrate the environmental issue. Keywords: Trypanosoma Cruzy. Chagas Disease. Epidemiology CPE-1534 During The Period From 2006 To 2010 Almeida, M.C.¹; Costa, B.G.S.¹; Santos, C.R.C.¹; Gomes, C.C.S.¹; Lorena Júnior, E.J.P.¹ ; Silva, K.D.M.¹; Pereira, 1. Departamento de Enfermagem-UFPE/ Brazil 2. Departamento de Ciências Biológicas, Universidade de Pernambuco- UPE/ Brazil Background: Venomous animals are those that have glands in Brazil are spiders, snakes, scorpions, caterpillars and authorized, so the only guarantee that there are no accidents is the prevention of its occurrence. Accidents caused

During The Period From 2006 To 2010 1. Departamento de Ciências Biológicas – UFPE/ Brazil 2. Departamento de Enfermagem - UFPE/ Brazil 3. Departamento de Ciências Farmacêuticas – UFPE/ Brazil 4. Faculdade Integrada da Vitória de Santo Antão - FAINTVISA/Brazil 5. Departamento de Ciências Biológicas - Universidade de Pernambuco/ Brazil 6. Departamento de Ciências Ambientais - UFPE/ Brazil 7. Departamento de Enfermagem – Universidade de Pernambuco/ Brazil Background: Chagas disease is a consequence of hu-

fusion, vertical, oral, accidental and vector, the last one Methodology: We used the National Information System gas disease reported during the period 2006 to 2010, ex-

For this study we used the Sistema de Informação Nacio-

and lethality of the victims. Results: During this period, 325.028 cases were reported, there was a predominance

promotion of actions to control and health education as well as decrease the severity, frequency of sequelae and lethality through the appropriate use of serum therapy. Keywords: Venomous animals. Accidents . Public health. CPE-1535 Current Situation Of Leprosy In Pernambuco State Costa, B.G.S.¹; Almeida, M.C.¹; Gomes, C.C.S.¹; Lorena Júnior, E.J.P.¹ ; Silva, K.D.M.¹; Pereira, M.A.S.¹; Melo, 1. Departamento de Enfermagem-UFPE/ Brazil Leprosy is an infectious disease of slow evolution, and

During this period it was noted 671 reports of the injury, It mainly affects the skin and nerves, leaving the person search invigorate the need to carry out regional studies, so they can take measures aimed at controlling every aspect of the population, thus, to have an adequate development of actions to control Chagas disease, is extremely important that Primary Care Teams and the Family Health teams incorporate into their work process surveillance activities 56

weakness and paralysis in the areas where the affected study aimed to identify the prevalence of leprosy cases in -

2011 5 (2)

collection for the years 2008 to 2011. We analyzed some of residence, deaths, cure and treatment dropout). During the period 11,961 cases were reported, of which 103 died -

3- Student of Biomedicine Maurice of Nassau – Brazil Introduction: Several authors consider that the assessment of the adequacy of the sample is the most important isolated component in quality assurance in Pap smear (Papanicolaou smear) result. The Bethesda system in the original versions included three categories of adequacy: satisfactory, unsatisfactory and a category designated initially “less

need for regional studies, raising account some aspects that treatment, requiring a resolute surveillance that provides greater control of the disease or at least the early discovery rosy and giving an opportunity to cure the sick. Keywords: Leprosy. Hansen’s bacillus. Mycobacterium leprae CPE-1536a Epidemiologic Review about Leishmanoise Tegumentar Cases in Children from the City of Vitória de Santo Antão Melo,G.P.¹; Patriota,H.S.¹; Morais, L.L.M.¹; Silva, P.K.F.¹; da Silva, P.K.F.¹; Santos,L.S.¹; Ferraz, I.C.¹; Melo, M.M.B.¹; Simôes, P.T.¹; Melo, R.O.¹; Almeida, M.C.¹ 1. Department of Nursing. Universidade Federal de Pernambuco - UFPE, Brazil. Introduction: Because it is a phase where the organism is dominates in the line of reasoning, the child many times presents chances to acquire parasitic diseases compared to adults. In this context the Leishmanoise Tegumentar is gaining room over this social class, disease characterized pal vector the straw mosquito, from the gender Lutzomyia, appearance of papules may evolve to ulcers with granular -

cases of Leishmanoise Tegumentar in children from 0 to

category and are now designated as “satisfactory” or “under to determine the prevalence in relation to the sample (satisfactory or unsatisfactory) the results of cytopathology in municipal health service in Campina Grande – PB. Methodology: This was a descriptive exploratory study with a quantitative approach, the data were collected on sults were as follows: of the 300 study results showed that

to educational lectures for professionals who deal with the collection and staining of cytological material. Keywords: Papanicolaou smear, satisfactory, unsatisfactory CPE-1538 Anorectal Citology As Screening Method Of Anal Intraepithelial Neoplasia (Ain) In Hiv-Negatives Women Castor, L. G1.; Nunes, M. J. G2.; Castor, M. G. F. C3; Medeiros, A. L. P. B4.; Bruneska, D5.; Pontes-Filho, N. T6. 1. Laboratório de Imunopatologia Keizo Asami/LIKA/UFPE, Brasil. 2. Universidade Federal de Pernambuco/Programa de Pós-Graduação em Patologia, Brasil 3. Universidade Federal de Pernambuco/ Hospital de Câncer de Pernambuco, Brasil 4. Laboratório de Imunopatologia Keizo Asami-LIKA, Brasil 5. Universidade Federal de Pernambuco/Centro de Ciências Biológicas, Brasil 6. Universidade Federal de Pernambuco/Centro de Ciências da Saúde, Brasil man Papillomavirus (HPV) can lead to the development transition zone of the anal canal it may lead to pre-cancer -

is realized the importance of creating political integrative strategies as campaign, projects and programs aiming to get rid of this pathology from this infant age, present in the research as of most risk. Keywords: Epidemiology; Children Health; Leishmanoise Tegumentar

of anal cytology as screening method to detect anal intraepithelial neoplasia (AIN). Methodology: 48 patients, attended in Pelvis Service from Cancer Hospital of Peranal canal and transition zone. After detection of clinical lesions, anorectal cells were collected with gynecological

CPE-1537 Quality Of Cytopathology In Campina Grande –Pb Oliveira, Stéphanny Sallomé Sousa1; Araújo, Thereza

-

Giovanni Tavares3. 1- Academic Biomedicine Maurice of Nassau – Brazil 2- Lecturer School of Nursing Paulista – Brazil

and good preservation of the samples. These samples

57

Experimental Pathology and Health Sciences

this context it is important to search for new therapies. cacy of the method. Additional studies with larger sample and comparison with other methods may determine the methanol in the same proportion to the extract methanoKeyords: Anal Citology, AIN, Human papillomavirus rotaevaporation. The dry extracts were re-suspended with CPE-1539 Antimicrobial activity of endophytic fungus Trichoderma harzianum isolated from Hancornia speciosa Gomes Chagas, M.B.O.1; Santos. I.P. 1; Silva, A.S. 1; Silva, C.M.A. M.S. 1; Lima, V.L.M. 2 1. Department of Mycology - UFPE, Brazil 2. Department of Biochemistry - UFPE, Brazil Background: Hancornia speciosa Gomes, known as manginterior of plants and among these, fungi, have attracted

mL. Were then added 30 µL of the extracts on disks (5 mm in diameter) sterile paper and transferred to Petri dishes, in triplicate, containing agar-Müeller-Hinton (MHA) me-

and is considered positive when the halos were more than 10 mm in diameter. Results: The ethanolic extract did not other microorganisms showed activity, varying the average

phytic fungus Trichoderma harzianum isolated from the (PDA) medium contained in Petri dishes, in triplicate, and ouraud (SAB) medium. After this period, were fragmented

halos of 16 mm to 31 mm in diameter. Conclusions: The extract methanolic showed more active against the test mipromising for future studies in order to evaluate its use in infection control. Keywords: H.speciosa; nosocomial infections. CPE-1540 Síndromes Hipertensivas Induzidas Pela Gestação E Repercussões Perinatais: Um Estudo De Revisão

triplicate containing agar - Müeller-Hinton (MHA) medi-

12.5 mm to 33 mm in diameter. Conclusions: The endo-

1. Acadêmica do curso de Bacharelado em Enfermagem pela Faculdade Maurício de Nassau. 2. Curso de Bacharelado em enfermagem da Faculdade Maurício de Nassau. Recife-PE, Brasil. Introdução: entre as doenças maternas que ocorrem no período gravídico, a hipertensão induzida pela gravidez é considerada uma das que mais efeitos nocivos provocam no organismo materno, fetal e neonatal, presente em 10 a tensivas induzidas pela gestação, seus fatores predisponen-

genic microorganisms. In future research, the results of

revisão de literatura de estudo transversal, descritivo. Para tanto, foram consultados materiais impressos, como liv-

-

estudo, onde foram selecionados 30 artigos com período

H.speciosa;

ternos predisponentes para as síndromes hipertensivas gestacionais e o desenvolvimento da mesma, repercutindo

moniae, Pseudomonas aeruginosa and the fungi Candida SAB medium. After 48 hours were taken the readings of halos were more than 10 mm in diameter. Results: All iso-

Keywords: Antimicrobial T.harzianum.

activity;

CPE-1539a Antimicrobial activity of extracts of stem bark from Hancornia speciosa against the most common microorganisms in nosocomial infections Chagas, M.B.O.1; Santos. I.P. 1; Silva, A.S. 1; Silva, C.M.A. M.S. 1; Lima, V.L.M. 2 1. Department of Mycology - UFPE, Brazil 2. Department of Biochemistry - UFPE, Brazil Background: The incidence of nosocomial infections is a 58

mostrou-se como principal fator de risco para seu desenvolvimento e neonatos pequenos para a idade gestacional foi a principal repercussão perinatal encontrada durante a sez de estudos referentes a essa patologia torna difícil o acesso aos conhecimentos que poderiam prevenir tais -

2011 5 (2)

mento adequado. Palavras-chave: hipertensão gestacional; eclâmpsia; pré-eclâmpsia. CPE-1545 Analysis Of Secretory Activity In Rats Under The Effect Of Derivatives Phthalimides Silva, T.L1;Jimenez, G.C1 ; Neto, J.F.S1; Souza, D.M.B1; Antunes, R.V2. 1. Departamento de Morfologia e Fisiologia Animal – UFRPE, Brasil. 2. Departamento de Química – UFRPE, Brasil. Introduction: Phthalimides represent an important group -

the intraperitoneal cavity. Animals in four groups received a type-oxadiazole Phthalimides (A, B, C, D), and another group served as control. At the end of nine days all animals were eviscerated after anesthetic depth with Xylazine spectively (intramuscular). Then the liquid was removed histopathological evaluation was performed on fragments of the tumor, liver, spleen, lung and kidney. Result: It was found that only Phthalimides A and B showed a tumor tistically in the control group. Histopathologic evaluation ter structural preservation compared to the control group.

effects of derivatives of oxadiazole-Phthalimides on the secretory activity in rat ileum, particularly the participation of chloride channels. Methodology: oxidiazole-ftalimídimechanisms of tumor development. Keywords: Tumor, inhibition, Phthalimides. 24 hours of fasting, were anesthetized with ketamine + -

CPE-1549 on growth of solid Ehrlich tumor Pinto, F. C. H.1; Souza, D. A. S.1; Costa, P. M.1; Da Silva,

ileocecal six loops were formed, each received one type of solution pH 7.2 in a volume of 20µL per compartment

R. D.2; Almeida, L. F.2; Tavares, S.2; Pinto, A. R.2 1. Federal University of Sao Joao Del Rei, Divinopolis, Brazil 2. Presidente Antonio Carlos University, Barbacena, Brazil Background: Aloe vera, also known as the true or medici-

animal left as control (Ringer pH 7.2). After a period of and magazines in your content evaluated, measuring up chloride ions. Results: All derivatives produced ftalimidicos secretion characteristic shape. Derivatives ftalimídicos A, B and C strongly promoted vascular congestion,

ever, there are evidences that Aloe vera extracts present genic effects of Aloe vera ethanolic extract on the solid Ehrlich tumor was evaluated. Methodology: A total of 18 Swiss female mice, two months old, were divided into three groups of 6 animals each: A, B and C groups. The

of chloride ions in concentrations similar, suggesting an of enterocytes. Conclusion:-oxadiazole derivatives evaluated ftalimidicos seem to affect paracellular transport of Aloe Vera ethanolic extract. Additionally, at the thirty the arachidonic acid cascade. Keywords: Phthalimides, secretion, rats. CPE-1545a Tumor Inhibition In Swiss Albino Mice Mus Musculus, Submitted To Derivatives Ftalamicos Silva, T.L1;Jimenez, G.C1 ; Neto, J.F.S1; Souza, D.M.B1; Antunes, R.V.2 1. Departamento de Morfologia e Fisiologia Animal – UFRPE, Brasil. 2. Departamento de Química – UFRPE, Brasil. Introduction: Cancer is a very common type of pathology improvement aimed at early diagnosis and effective treatment, quality of life. The phthalimides represent a new

Ehrlich tumor cells (0.05 mL) on the footpad. The tumoral ing a digital pachymeter. At the sixty day, the animals were -

differences among tumors of the groups. The metastasis in popliteal nodes of the tumor occurred in all animals. Conclusion: Aloe vera ethanolic extract potentiated and accelerated the tumoral growth. In such case, future studthe activity of the tumor. Keywords: Ehrlich tumor, Aloe vera, histopathology. CPE-1550

with four different types of synthetic derivatives of oxa-

mammary neoplasms in cats E.L.4; Peixoto, C.A.4; Neves, C.O.5; Melo-Júnior, M.R. 5; Porto, A.L.F.6 59

Experimental Pathology and Health Sciences

1. Centre for Animal Experimentation, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Brazil. 2. Veterinary Hospital, Universidade Federal Rural de Pernambuco, Brazil. 3. Laboratory for Clinical Research in Dermatozoonoses, Instituto de Pesquisa Clínica Carlos Chagas, Fundação Oswaldo Cruz, Brazil. 4. Laboratory of Ultrastructure, Department of Entomology, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Brazil. 5. Departament of Pathology, Universidade Federal de Pernambuco, Brazil. 6. Department of Morphology and Physiology, Universidade Federal Rural de Pernambuco, Brazil. Background: The vast majority of mammary tumors in cats These tumors have poor prognosis, since such tumors are -

the ovariectomized animals presented higher serum levels

-

ment. Conclusions: alpha-tocopherol supplementation

point of view histochemical and antigenic spontaneously occurring mammary tumors in cats. Methodology: Thirtyhistological sections were stained with Masson Trichrome and Periodic Acid Schiff (PAS) to evaluate the deposition of collagen and glycosaminoglycans associated with tumors, respectively. To perform the immunohistochemistry assays were used anti-Ki-67, anti-PCNA and anti-Bcl-2.

Keywords: alpha-tocopherol; ovariectomized rats; osteopenia CPE-1554 one derivative in carrageenan-induced acute injury in mice lung

of interstitial collagen and PAS + cells in mucinous cara higher markup to decreasing reactivity with the PCNA marking is only for PCNA and Ki-67. Conclusions: The data suggest that malignant neoplasms in cats have intense proliferative activity, indicating a poor prognosis. Keywords: Mammary neoplasia, Felis catus, Immunohistochemistry.

E.L.1; Donato, M.A.1; Silva, T.G.3; Lima, M.C.A.3; Pitta, I.R.3; Galdino, S.L.3; Peixoto, C.A.1,2 1. Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ, Brazil 2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/ MCT, Brazil 3. Universidade Federal de Pernambuco/Departamento de Antibióticos - Brazil Background: Peroxisome proliferator-activated receptors

CPE-1551 The Development Of Osteopenia In Adult Ovariectomized Rats Schiavotelo, Nathalia L.1; Camargo, Vânia B.1; Santos, Amanda M.1; Gonçalves, Francisca de A. M.2; Ruffoni, 1. Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, Brasil 2. Centro de Estudos Ambientais, Universidade Estadual Paulista, Brasil 3. Departamento de Ciências Fisiológicas, Universidade Federal de São Carlos, Brasil Background: Several studies have aimed to identify aljectives: This study was designed to determine the effect of alpha-tocopherol supplementation in the experimental development of osteopenia in adult ovariectomized rats. Methodology: 36 Wistar rats aged 120 days were

Two weeks after ovariectomy, treatment was initiated with days, or only the vehicle (sterile peanut oil, 0.2 ml). Ninety days after treatment the serum levels of calcium, phosphorus and alkaline phosphatase were measured; the mineral 60

injetion at the level of the left sixth intercostal space. Saline injected into the pleural cavity. At 4 h after the injection pors. Lung fragments were processed for light microscopy and immunohistochemical assays. Results: As expected, no saline-treated animals. Immunohistochemical analyses did cal analysis of the CAR group showed increased cellularity, emphysema. The CAR group presented intense staining for cyclooxygenase (COX-2) compared with control mice. Increased COX-2 expression is associated with increased levels of downstream enzymes required for prostanoid program Gimp 2.6 software. Conclusions: According to to clarify the role of RA-4 on the molecular mechanisms

2011 5 (2)

-

Six healthy volunteers each received a single oral dose of 0.5 mg digoxin, from Glaxo Wellcome S.A, Brazil. Blood

CPE – 1555 Antitumor effects of 8-methoxypsoralen in an in vitro model of glioblastoma

1.50, 2.00, 3.00h, 4.00, 8.00, 12:00, 24:00, 48:00, 72:00,

tory.

M.L.V.2; El-Bachá, R.S.1 1. Laboratory of Neurochemistry and Celular Biology, Health Sciences Institute - UFBA - Brazil 2. Gonçalo Moniz Research Center - Fiocruz - Bahia - Brazil

-

1.819, 1.137, 0.774, 0.640, 0.357, 0.268, 0.263, 0.058, 0,

time, nevertheless the impact of technological advances new therapeutic approaches, treatments and drugs are roliferative and apoptosis-inducing activities of the furanocoumarin-derivate 8-methoxypsoralen (8-MOP), a drug used for skin diseases treatment. Methodology: Rat glioma trocytes were used in tests with increasing concentrations and colony formation assay. Cell morphology was accessed used for elucidation of the kind of cell death. Results: -

(52.24), 17.7800 and 17.1480 (92,53), 10.1066 and 10.6012 (91,13), 0.1518 and 0,1493 (98,23) respectively. Concluin Linux software. Keywords: Digoxin, Linux, pharmacokinetics. CPE-1556a Comparative evaluation of haemoglobin using N/10 hydrochloric acid versus automated method Bezerra da Silva, J.F.1; Oliveira, TN1; Souza Filho1, S.D.; Alves, A.Q.2; Alves, A.J.1 1. Departamento de Ciências Farmacêuticas. Universidade Federal de Pernambuco, Brazil, 2. Faculdade Pernambucana de Saúde, Brazil -

diameter of the colonies. In addition, 8-MOP increased MTT assay, in GL-15 cells. Conclusions: 8-MOP presented antiproliferative and apoptosis-inducing activities in glioma cells. Additionally, it did not affect normal astrocytes to the extent that affected tumor cells. Then, 8-MOP is a good prototype of new anti-glioma drug as well as a pos-

not have such equipment; they are expensive and require -

-

Keywords: Cancer; psoralen; glioma CPE-1556 Pharmacokinetics of a Single Dose of Digoxin in Healthy Volunteers using Linux Systems Oliveira, T.N.1; ALVES, A.J.1; ALVES, C.J.4; ALVES, A.Q.2; GÓES, A.J.S.1 Carvalho Neto, J.L.3; Aquino, T.M. 1 1. Department of Pharmaceutical Sciences, Federal University of Pernambuco Stat, Brazil, 2. Faculty of Health of Pernambuco Stat, Brazil, 3 Hospital Mermorial Guararapes, Brazil. 4 Maxiclinic Medical, Brazil

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cardiotonic glycoside worldwide for the treatment of congestive heart failure and atrial arithmias. Even though it is known as a drug with a narrow therapeutic index. Routine two software. Methodology: The protocol complied with the provisions of the Declaration of Helsinki (1964) and

compared to automated method. We consider this as clinias the mean difference ±2 SD and the calculated lower and dl respectively. Both these suggest that the methods agree

the Ethics Committee from Hospital Memorial Guara61

Experimental Pathology and Health Sciences

Keywords: haemoglobin, hydrochloric acid CPE-1557 Prediction Of 3d Structure Of Protein Messenger Of Ovarian Immunosuppressants Ascaris Suum ga, C.G.O1; Pontes-Filho, N T3; Souza, V.M.O1. 1. Laboratório de Imunopatologia Keizo Asami - LIKA/UFPE, Brazil. 2. Department of Biochemistry, UFPE, Brazil. 3. Department of Pathology, LIKA/UFPE, Brazil. Background: Extract of Ascaris suum adult worms has proteins with regulatory activities that can modulate the immune response to antigens homologous or heterolo-

have assessed the longevity of this enhancement and whether it is dependent on the administration route. For S. mansoni), on the 60th day had their estrus synchronized infected mothers (Control). For analyzing of the administration route three groups were added: animals controls ing weaning in two doses: 0,12µg(SEA.1) or 1,2µg(SEA.2). The third group received oral SEA, after weaning (21 days) at the dose of 6 µg(SEA-oral).On the 45th day, all ani21th, 28th, 35th and 68th days post-immunization. It was

of this extracts with immunoregulatory action in a model the study was the prediction of protein structural Messenger immunosuppressive Ovarian (PMIO) of A. suum and residual analysis of phosphorylation targets. Methodology:

group was more evident from the 28th day. However, for the animals which had received milk from infected, fourfold IgG1 level was detected yet on the 8th day and it kept until the 28th day. For the animals sensitized with SEA,

National Center for Biotechnology Information (NCBI), was analyzed for the phosphorylation sites (program NetPhos - prediction of phosphorylation on serine, threonine

animals. Regarding the titles of IgG2a, the increment was

(NetPhosk) then the structural prediction was made in

route, in the lactation phase, induced an early production

dues in most favoured regions (MFR), additional allowed regions (AAR), generously allowed regions (GAR) and disallowed regions (DR). The model was also analyzed with

the IgG1 synthesis. Keywords: Schistosomiasis experimental; Breastfeeding; SEA.

quality compared with protein structures deposited in the Protein Data Bank (PDB). Results: Found 11 residues of serine undergo phosphorylation (positions 29, 67, 77, 93, 124, 191, 286, 288, 290, 301, 397), 8 threonine (128, 178, 189, 200, 216, 246, 282 and 363) and 10 tyrosine (35, 61, 175, 186, 197, 222, 231, 269, 339 and 431). NetPhosk indicated the involvement of protein kinase C activation in PMIO acting on the serine residue at position 124. The 3D model generated showed high stereochemical quality with overall quality has resulted in Z-score (-2.92) in accordance netic Resonance. Conclusions: Study of the structure and the mechanisms involved in activation of PMIO result in knowledge for future immunotherapy strategies related to autoimmune diseases. Keywords: Ascaris suum; Protein; Immunomodulation. CPE-1557a Kinetics Of The Anti-Ovalbumin Antibodies Production In Adult Mice Sensitized With Antigens Of Schistosoma Mansoni In The Breastfeeding querque, M.C.P.A1; Costa, V.M.A.1; Souza, V.M.O1. 1. Laboratório de Imunopatologia Keizo Asami - LIKA/UFPE, Brazil. 2. Biotherium of Centro de Pesquisa Aggeu Magalhães, Fiocruz, Brazil. Schistosoma mansoni infected pregnant women are common in endemic areas. Expositions to parasitic component in sponse to heterologous antigen. Experimental studies in 62

CPE-1559 New Candidates for anti-Schistosoma mansoni: Evaluation of Biological Activity Santiago, EF1; Leite, ACL1; Moreira, DRM1; Oliveira, SA2; Barros, AF 2; Silva, AL3 1.Laboratório de Planejamento e Química Medicinal - UFPE, Brazil 2. Laboratório de Imunopatologia – FIOCRUZ, Brazil 3.Laboratório de Planejamento e Síntese de fármacos - UFPE, Brazil. of Africa have showed resistance to praziquantel, this is still the drug of choice worldwide. In the new scenario, it is necessary to study new drugs with activity schistosozone. Methodology: We used 50 mice of the strain Swiss the portal system for the recovery of worms. The parasites

After a period of two hours of adaptation, three phenyl-

motor activity and mortality. Resultados: The molecule -

2011 5 (2)

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2. Academic Center of Vitória - Federal University of Pernambuco, Brazil Background: The comet assay is a simple, sensitive and quantitative method for measuring DNA single and strand ics and process of DNA repair. Agricultural workers are prone to occupational exposure to a variety of pesticides which represents a potential risk to human health, includ-

Keywords: schistosomiasis, thiosemicarbazone, study “in vitro” CPE-1561 Vegetables Show DNA Protector Effect Even Contaminated With Pesticides: A Study By Micronucleus Test Silva, E. J.1; Dias, S. A.2; Freitas, E. M. S.2; Adam, M. L.2; And Chagas, C. A.1 1. Postgraduate Program in Human Health and Environment, Academic Center of Vitória - Federal University of Pernambuco, Brazil 2. Academic Center of Vitória - Federal University of Pernambuco, Brazil -

sonal Protection Equipments (PPE) in the damage detectogy: The study included 54 individuals, 31 farmers and 23 non-exposed controls. The farmers were evaluated in two day-use of PPE during the same activities. DNA dam-

toxic damage, H2O2 was used as positive control. Regroup (ID-0.14±0.13; FD-10.38±10.58) and control (ID-

nation. Methodology: Eighteen Wistar rats were divided Group 1 was water control, Group 2 received 1 daily dose -

- ITEP - to identify what pesticides were present in these to carry out the Micronucleus Test. Results: Many different then were found in concentrations lower than the Accepton micronucleated leukocytes when compared to Group 2 (p > 0.05 - Kruskal-Wallis Test). Antioxidants, naturally tween Groups 1 and 2. A non dose-dependence on that an2 and 3. Conclusions: Despite the presence of pesticides, genic effect in the experimental design carried out. Keywords: Agrochemicals; DNA Damage; Antimutagenicity. CPE-1561a Genotoxicity In Agriculture Workers Exposed To Pesticides: Effect Of Individual Habits And Use Of Personal Protection Equipments In Dna Damage And Chagas, C. A.¹ 1. Postgraduate Program in Human Health and Environment, Academic Center of Vitória - Federal University of Pernambuco, Brazil

(0.06 ± 0.06) and FD (4.37 ± 4.12) in the Post-PPE when ney test). Such factors as Smoking, alcohol intake, time of exposure, age and sex had no effect on the ID and FD in all experimental groups (P>0.05). Conclusion: Our results of DNA damage in farmers. The use of PPE reduced the exposure to pesticides and consequently the damage was repaired. Keywords: Agrochemicals; Comet Assay; Biomonitoring CPE-1563 Histopathologic Characterization Of The Neuromuscular Mutation Fraqueza (Frq) Induced By Enu In Mice Policarpo, A.C.F.1,2; Silva Silva, D.A.1; Massironi, S.M.G.3; Guénet, J.L.4; Arantes, R.M.E.5; Godard, A.L.B.1 1.Laboratório de Genética Animal e Humana, Instituto de Ciências Biológicas - Universidade Federal de Minas Gerais, Brasil. 2. Fundação Comunitária de Ensino Superior de Itabira – FUNCESI, Brasil. 3. Departamento de Imunologia, Universidade de São Paulo, Brasil. 4. Unité de Génétique dês Mammifères, Institut Pasteur - França. 5. Laboratório de Neuro-Imuno Patologia Experimental, Universidade Federal de Minas Gerais, Brasil. Mouse mutagenesis is a key tool for studying gene funcmouse colonies. The fraqueza mice mutant phenotype mutation is deleterious and inherited as an autosomal D1Mit320 (18.64 cM). Fraqueza mice present progressive loss of motor coordination and muscle strength at two weeks postnatal, associated with writhing and contraction at 12 weeks postnatal. From the molecular and phenotypic the gene Dystonin (Dst, Bpag1) as a candidate for the mu63

Experimental Pathology and Health Sciences

1. Health Sciences Center, Federal University of Paraíba - Brazil at characterizing the mutant phenotype fraqueza through techniques of complete necropsy, histological evaluation of spinal cord and nervous and muscle tissues, and clinical characterization of the phenotype. Morphometric analysis evidenced smaller mean muscular citoplasmatic area in mutant mice compared to their paired controls evaluated at 3, 7 and 11 weeks postnatal. Analysis of histological sections of muscles presented slight muscle atrophy in fraqueza mice, with increase in endomysial conjunctive tissue and slight vascular neoformation. There is also a slight increase in nerves and spinal ganglia cellularity and decrease in terations. The results may indicate ultrastructural injury neurodegenerative diseases and we intend to validate this model for the development of therapies for human dis-

tives: This study aimed to assess the incidence of intestinal parasites in patients treated at the Polyclinic of Cajazeiras, PB. Methodology: The parasitological tests of the samples Municipal. The 517 samples were analyzed in the period mentation technique, in order to determine the frequency of protozoan cysts and helminth eggs and larvae. Results: tive. As the protozoa and the species most frequently was

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Keywords: animal models, mutagenesis, neurodegenative disease, histopatology.

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CPE-1565 Incidence Of Intestinal Parasites In The Sandy Beaches Of Paraíba Oliveira Filho, A. A.1; Fernandes, H. M. B.1; Alcântara, N. D. F.1; Freitas, F. I. S.1 1. Health Sciences Center, Federal University of Paraíba - Brazil Background: The infectious and parasitic diseases are still gions and poorer populations, however, despite the great technological advances of the high standard of education, good nutrition and good health, even developed countries

dence of intestinal parasites, especially protozoa cysts that sures such as improved sanitation and hygiene education continued as well as new studies parasitological, after the implementation of these measures, if necessary, and are

few studies that address the levels of infection of intesti-

the month of May 2010, totaling 112 samples. The parasitological tests used in this study were analyzed accordthe spontaneous sedimentation technique and the method

Keywords: Parasitological tests, Polyclinic of Cajazeiras, Intestinal parasites CPE-1567 Histopatological Changes Induced By Bothrops Leucurus Venom On Mice Skeletal Muscle After Experimental Poisoning 1. University of Pernambuco, Brazil. 2. Federal University of Pernambuco, Brazil. 3.San Francisco University, Brazil. 4.State University of Campinas, Brazil -

the presence of at least one species of intestinal parasite. Samples of wet sand had a higher rate of positivity for the helminths. Conclusions: The high rate of parasitic forms research reveals the need for improvements, through the cases, amputations. In the present study we examined the prevention and health education, as an infection with intestinal parasites can cause damage not only to individuals

the mice gastrocnemius muscle. The anesthetized mice received the intramuscular injection of venom (100 µL, 80

of the population. Keywords: Parasitological tests, Paraiba Coast, Intestinal parasites.

group) on the dorsal-lateral region of the right paw. After 3hs, 6hs, 1 day, 3 days, 7 days and 21 days after inocula-

CPE-1565a Evaluation Of The Incidence Intestinal Parasites In Patients Treated At The Polyclinic Of Cajazeiras, Pb Oliveira Filho, A. A.1; Moura, D. V.1; Fernandes, H. M. B.1; Freitas, F. I. S.1 64

light microscope and transmission electron microscope.

2011 5 (2)

The ultrastructural analysis showed the disorganization of the sarcomeres and mitochondrial damage on the muscles within 24 h post-venom injection. The presence of

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third day after venom injection. At 21th day we noted the restoration of muscle cytoarchitecture except for the presence of interstitial edema. Our results proved the myotoxic action of B. leucurus venom and that the satellite cells participated to the muscle regeneration processes. Keywords: Bothrops leucurus, Muscle Skeletal, Myotoxicity

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CPE-1569

Keywords: L-fucose, vessel, prostate.

Chromogenic In Situ Hybridization In Breast Cancer Samples Silva Filho, J.L.Q.1; Cartaxo, M.F.S.1; Lima, L.R.A.1; Cavalcanti, C.L.B1; Ramalho, E.A.V.F.1; Beltrão, E.I.C.1,2 1. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, Brazil; 2. Departamento de Bioquímica, CCB/UFPE, Brazil. Background: Breast cancer is the second most common

CPE – 1571 Luminescent Probes for Breast Tissues: the Use of Europium (III) Cryptate Conjugated to Lectins in Diagnosis Brandão, J.M.1; Bandeira, A.R.G.1, 2; Vasconcelos, J.L.A.1; Cavalcanti, C.L.B.1; Júnior, S.A.2; Beltrão, E.I.C.1, 3 1 Grupo de Pesquisa Biomarcadores no Câncer - LIKA/UFPE, Brasil. 2 Departamento de Química Fundamental – DQF/UFPE, Brasil 3 Departamento de Bioquímica – CCB/UFPE, Brasil

cer in women. In this context, the study of some markers may help the understanding disease progression, as well as planning the most appropriate therapy. c-myc gene is involved in cell differentiation, apoptosis, angiogenesis and immortalization playing central role in the tumorigen-

pathological parameters in CDI samples. Methodology: tion of the c-myc was detected in 2 out of 10 cases. These two cases were of women under 50-year-old and tumours fonodal invasion with histological grade III. Among negative patients 5 were older than 50-year-old, 7 had tumours

for diagnosis of cancer has conducted research in pursuit of this goal. Histochemical studies have shown that quanface glycoconjugates during neoplastic processes. Lectin histochemistry is a tool that can auxiliaries in the diagnosis of these pathologies, regarding changes in their saccharide content. Europium (III) cryptate, macromolecules with lustudy proposed the use of the conjugate lectins-europium cocode of glycoconjugates on the cell surface of trans-

and only one histological grade I, 2 were grade II and 5 were grade III. Conclusions: The present results show that than 50 years with high histological grade and linfonodal invasion. Indicating that this oncogene is associated with aggressive tumors. Keywords: Hibridization in situ; c-myc; breast cancer. CPE-1570 Expression of L-fucose in blood vessels as biomarker for human prostate diseases Vasconcelos, J L A1, Brandão, J M1; Cavalcanti, C L B1; Lima, A L R1; Beltrão, E I C1,2. 1. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, Brazil 2. Department of Biochemistry – CCB/UFPE, Brazil Background: The prostate gland is the male reproductive

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Results: Normal tissues showed no emission in all samples. FIB samples showed staining only for Con A-Eu(III)crip Eu(III)crip (average emission of Con A, WGA and PNA Samples luminescence intensity showed different patterns clusions: This work suggests the use of lectins-Eu(III)crip

Fucosylation is a mechanism which functions to insert the seen as one important factor in the development of varisies diagnosed with Benign Prostatic Hyperplasia (BPH -

sis as an auxiliary tool. Keywords: europium cryptate, lectin, breast tissues. CPE-1573 Mycobacterium leprae DNA detection in nasal secretion of patients with leprosy from the Center of National Reference in Sanitary Dermathology Dona 65

Experimental Pathology and Health Sciences

Libânia (CDERM) – Fortaleza, Ceará Marques, LCM 1; Lima, LNGC 1; Assis, LC 1; Dantas, JCP 1; Kerr, LRS 1; Frota CC¹. 1. Laboratory of Mycobacteria, Medicine School, Federal University of Ceará, Brazil rium leprae, which dissemination occurs through the nasal secretion and affects mainly the skin and nerves. Ceará ranks as tge 10th in overall detection rate in the country.

-

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in nasal secretion from individuals with leprosy. Methwho were cared in the Center of National Reference in cases from the total of participants. In general, men had a

women the highest positivity was from 31 to 40 years old

developing leprosy than women. Conclusion: The sensitivity of the nested PCR for the RLEP2 region is very high

histochemistry using haematoxylin and eosin in skin diagnosis. Keywords: chemiluminescence, histochemistry, lectins, carbohydrates, cutaneous tumors. CPE-1576 Experimental invasive pulmonary aspergillosis: histopathological analysis of lungs from immunocompetent and neutropenic animals infected by Aspergillus fumigatus strains with different patterns of production of elastase Silva, R.L.H.1; Rosa-Milani E.2; Brunaldi, M.O.1; Maffei, C.M.L.2 1. Departament of Pathology and Forensic Medicine. Faculty of Medicine of Ribeirão Preto - University of São Paulo. 2. Departament of Cellular and Molecular Biology and Pathogenic Bioagents. Faculty of Medicine of Ribeirão Preto - University of São Paulo. Background: Invasive Pulmonary Aspergillosis is an opwhich affects mainly immunocompromised patients. Neutropenia is a prerequisite for hematogenous dissemination

Keywords: nasal mucus, leprosy, PCR CPE-1575 Chemiluminescent Quantitative Lectin Histochemistry Determination Of Cutaneous Tumors Glycophenotype Lima, L.R.A.1, Bezerra, M.F.1, Silva, L.P.B.G.1, Almeida, S.M.V.1, Cavalcanti, C.L.B.1, Beltrão, E.I.C.1, Carvalho Júnior, L.B.1 1. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, Brasil Background: Chemiluminescence assay is a promising and effective technique for quantitative analysis of proteins,

associated with invasiveness is the production of elastase the lungs of immunocompetent and neutropenic animals and strong (1753) production of elastase. Methodology: ally with monoclonal anti Gr-1 (induction of neutrophil after different periods (24, 48 and 72 hours). Immunosections that were stained with Hematoxylin and Eosin

in their glycosylation patterns when compared to their normal counterparts. Lectin histochemistry is an effec-

(W). Results: Histopathologic analysis of lungs stained -

and its functional consequences regarding glycophenotype work aimed to develop a chemiluminescent lectin histo-

24 and 48 hours post-infection in neutropenic animals

in cutaneous tumors. Methodology: Con A and PNA were Sephadex G-25 chromatography. Lectin-AE conjugates were assayed for haemaglutinatin activity and chemilumiwith conjugates, separately, and chemiluminescent reac-

66

sue fungal load. During this period there was no evidence These results indicate that neutropenia is an essential factor in the invasiveness of the fungus and suggest that the virulence of A. fumigatus is multifactorial. Financial Support: FAPESP and FAEPA Keywords: Experimental invasive pulmonary asper-

2011 5 (2)

gillosis, neutropenia; elastase. CPE-1577 Establishment and Partial Characterization of a Cell Line from a Human Glioblastoma Lima, R.M.F1; Sampaio, G.P.2.; De Oliveira, D.M1.; ElBachá, R.S.1 1. Laboratory of Neurochemistry and Cell Biology - Federal University of Bahia, Brazil 2. Laboratory of Immunology and Molecular Biology - Federal University of Bahia, Brazil -

The cell line ESP10 was derived from a sample of tumor

protein (GFAP), oligodendrocyte transcription factor 2 (olig-2), proliferating cell nuclear antigen (PCNA) and huhistochemistry, immunocytochemistry and cell cytometry. cellular pleomorphism. DTCP average was 30 hours. The SIONS: This new cell line of GBM showed a predomi-

aggressiveness and invasiveness. Characterization studies Keywords: human glioblastoma, characterization, cell line CPE-1579 New acridine derivative conjugated to lectin as chemiluminescent probe for detection of tumor biomarkers Almeida, S.M.V.1; Lima, L.R.A.1; Bezerra, M.F.1; Silva, L.P.B.G.1; Cavalcanti, C.L.B.1; Lima, M. C. A.3; Galdino, S.L.3; Pitta, I.R.3; Beltrão, E.I.C.1,2; Carvalho Júnior, L.B.1,2 1. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Brasil. 2. Departamento de Bioquímica, Centro de Ciências Biológicas (CCB) – UFPE, Brasil 3. Departamento de Antibióticos, Centro de Ciências Biológicas (CCB) – UFPE, Brasil. Background: There is an increasing interest and demand

with conjugate, separately, and chemiluminescent reaction accomplished. Results: All analyzed tumour types, KA

Even showing a weaker chemiluminescent emission than

served with DMAE. These results indicate the use of IPKey-words: chemiluminescent, acridine derivative, skin cancer CPE-1580 mavirus In Anal Canal Samples Of Hiv-Negatives Women Nunes, M. J. G1., Ekert M.H.F2., Castor, L. G3.; Castor, M. G. F, C4.; Bruneska, D5.; Pontes-Filho, N. T6. 1. Programa de Pós-Graduação em Patologia/UFPE/CCS, Brazil 2. Programa de Pós-Graduação em Biologia Aplicada à Saúde/ UFPE/LIKA, Brazil 3. Laboratório de Imunopatologia Keizo Asami/LIKA, Brazil 4. Hospital de Câncer de Pernambuco, Brazil 5. Departamento de Bioquímica/UFPE/CCB, Razil 6. Departamento de Patologia/UFPE/CCS, Brazil Background: Human papillomavirus (HPV) comprises more than 100 viral types, some of which infect the anogenital tract and lead to the development of Anal Intraepithelial Neoplasia (AIN). The persistence of HPV infection is considered a risk factor for development of squamous sample using polymerase Chain Reaction (PCR) and microarray. Methodology: The study was conducted with 26 patients, attended in Pelvis Service from Cancer Hospital cells samples were collected from anal canal. Total DNA was extracted for HPV detection through of PCR, using ray using PapilloCheck® HPV-screen DNA-chip to identify 06 genotypes low-risk and 18 genotypes high-risk. Results: The molecular diagnosis through PCR detected the PapilloCheck® analysis revealed HPV6 (low-risk) as the

ated the potential application of a new acridine derivative (IP-81) conjugated to Concanavalin A (Con A) lectin as G-25 chromatography. Con A-IP81 conjugate was assayed for hemagglutination activity and chemiluminescence (ex-

with different HPV genotypes. Among negative samples Conclusions: The results showed that PapilloCheck® diagnostic was effective to identify HPV in anal samples, and 67

Experimental Pathology and Health Sciences

progress to cancer. Key-words: Anal Intraepithelial Neoplasia (AIN), human papillomavirus, genotyping CPE-1580a Histomorphologic Analysis Of The Heart Oh Rats Submitted Malnutrition To Different Ages Da Costa, C.P.4; Pontes-Filho, N.T.3 1. Master student from Programa de Pós-Graduação em Patologia/ UFPE/CCS 2. Programa de Pós-Graduação em Farmacologia/UFPR, Brazil 3. Departamento de Patologia – CCS/UFPE, Brazil 4. Departamento de Fisiologia e Farmacologia, UFPE, Brazil Background: The normal function of an organ or of a system depends of adequate nutrients intake. As several uate the morphological and histological changes of heart

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of 30 minutes per 3 weeks). At the age of 35-45 days, aniwas colleted, weighed, measured and processed for histology. The sections, stained with haematoxylin and eosin, were captured (Motic) and analyzed (Image J) in order to calculate mean diameter (100x) of gastrocnemius muscle mean±standard deviation. Body weights (g) in the day of for ArS, 126.53±17.25 for WE and 143.95±12.74 for WS. respectively: 0.4881±0.0766, 0.0041±0.0002, 1.93±0.16 for ArE; 0.6859±0.1087, 0.00468±0.0004, 2.13±0.15 for ArS; 0.6110±0.1396, 0.0044±0.0006, 2.28±0.36 for WE; 0.6357±0.0731, 0.0042±0.0003, 2.03±0.12 for WS. Mean Diameter (µm) was 30855±2481 for ArE, 35784±3037 for ArS, 31670±2656 for WE and 28442±6140 for WS. Conclusions: According to these results, physical exercise of L-Arginine leads to muscle hypertrophy, independently muscle weight and length. Keywords: L-Arginine, exercise; skeletal muscle

period. The histogical analysis was performed in transversal sections of ventricles stained with Hematoxylin-Eosin (HE) and Picrosirius Red. The morphological data was

the cardiomyocytes from 3 and 5 months age rats from MG group. However, the changes were more intense in 5

CPE-1581a Effects Of Low Frequency Tens With Electrodes On Acupoints On Cold-Induced Pain Melo, M.P.M¹.; Montenegro, E. J. N¹ 1.Federal University of Pernambuco, Brazil ences negatively the activities of daily living of an individual. Nowadays, many therapies are used to relieve the pain, especially transcutaneous electrical nerve stimulation uate the effect of acupunture-like TENS (low frequency) located on acupoints TA 5 and CS 6 and the effect of

sis areas replacing the injured heart tissue, indicating repair process. Conclusion: The results revealed that multifactorial malnutrition interfered in cardiac organogenesis during post-weaning development, leading to histomorphological mance in adulthood. Key-words: malnutrition, heart, rat CPE-1581 Effects of amino acid L-Arginine and physical exercise on skeletal muscle of young rats Melo, M.P.P¹.; Vasconcelos, A.C.S.¹; Monteiro, H.M.C¹.; L.M.S.S¹. 1. Federal University of Pernambuco, Brazil Background: Physical exercise can promote many adaptations in skeletal muscles. In addition, the amino acid L-Arginine is related to improvement in exercise performance of L-Arginine and physical exercise during development. Methodology: 24 Wistar males rats were divided into four groups according to orogastric administration of L-Argi-

68

genders, randomly allocated, were divided into 2 groups: TENS with electrodes on acupoints TA 5 and CS 6 (A group) and the sham TENS (P group). In group P, the electrodes were placed on points not related to the induced with cold water (2-40C) during 20 minutes in order to inment (Electroanalgesy) during 20 minutes. The latency and after the treatment. These parameters were statistically sults were expressed as mean±standard error. The latency ± 4.72 during, 36.29 ± 3.12 after treatment. The latency ± 6.41 during, 44.92 ± 7.93 after treatment. Conclusions: According to these results TENS on acupoints TA 5 e threshold, during and after the treatment, comparing them therapy in treatment of algesic processes. Keywords: acupunture-like TENS, placebo, acupoints. CPE-1582 Association between the type of cervical injury and

2011 5 (2)

Human Papillomavirus genotype in Olinda, Brazil jo, R.F.F.4; Pontes-Filho5, N.T.; Lima-Filho, J.L.4; Bruneska, D.4. 1. Programa de Pós-Graduação em Biologia Aplicada à Saúde/ LIKA/UFPE 2. Programa de Pós-Graduação em Patologia/CCS/UFPE 3. Programa de Pós-Graduação em Biologia Aplicada à Saúde/ LIKA/UFPE 4. Departamento de Bioquímica/CCB/UFPE 5. Departamento de Patologia/CCS/UFPE Introduction: According to the World Health Organization, HPV infection is the major risk factor for develop-

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area. The data were compared using the Mann-Whitney

mammary gland, the cellular proliferation was increased

of cases. Currently the Pap test (Pap smear) is considered effective in early diagnosis and prevention of invasive term has slight proliferative effect on the epithelial cells als and Methods: The cross-sectional study was conducted with 241 women, ranging from 18 to 60 years, attended in underwent the examination and those cytology specimens Results: Through the Pap test it was shown that only 7 tween the type of cervical injury and genotype showed that HPV16 was the most prevalent when associated with low-risk genotypes 6, 11 and 35 were associated with lowgrade lesions, also in young patients. On the other hand genotypes 52, 66 and 82, considered high risk, were associated with atypical cells, conferring risk of evolution to cervical injury. HPV52 was the most prevalent in samples

Keywords: tibolone, mammary gland, histomorphometry Sources of research support: OFFICILAB, CAPES, CPE-1583a Mammary Gland Histomorphometry of Rats Treated With Estradiol Valerate And Norethisterone Enanthate Guzmán-Silva, MA 1; Henriques, HN 1; Couto BB 1; Azeredo VB 2; Pantaleão, JAS 3. 1 Pathology Department, 2 Nutrition and Dietetics Department, 3 Maternal and Child Department, Fluminense Federal University, Niteroi/RJ, Brazil. -

Conclusions: The high prevalence of the HPV16 in young in their responses to diverse ratios of these two steroids. prevention strategies to avoid cervical cancer, thus improving on treatments. Keywords: Papillomavirus, cervical injury, genotyping. CPE-1583 Histomorphometric Changes In Mammary Glands Of Female Rats Treated With Tibolone Guzmán-Silva, MA 1; Henriques, HN 1; Carvalho, ACB 1; Pantaleão, JAS 2. 1 Pathology Department, 2 Maternal and Child Department, Fluminense Federal University, Niteroi/RJ, Brazil. hormone replacement therapy and the exact mechanisms involving sexual steroids in the mammary cancer risk are not well-known. Aim: Analyze the mammary parenchyma of ovariectotime. Methods: Bilateral ovariectomy was performed on 14 rats weighing 250g. After 30 days, they were randomly divided

cervical displacement. Histological sections of thoracic matoxylin-eosin and the histomorphometric analysis was performed on images of each histological section merged with a 100-dot grid to estimate the area fractions for each -

tomorphometry. Methods: Fifteen Wistar female rats, aged eight weeks and weighing in average 250g were randomly divided in three of Mesigyna® (norethisterone enanthate and estradiol of sterile water. Treatments were performed on days 0, 7, 14, 21, and 28 of the experiment. Thoracic mammary logical sections were stained with haematoxylin-eosin and histomorphometric analyses were performed on images of each histological section merged with a 100-dot grid. Points coinciding with the desired structure were counted allowing to estimate the area fractions for each rat as acini fraction in the mammary gland (DAFM), epithe-

Results: The thoracic mammary gland showed an increase 69

Experimental Pathology and Health Sciences

the administration of norethisterone enanthate and estraferentiation and enlargement of the lumen, which leads to Keywords: estrogen, progestogen, mammary gland, histomorphometry. Sources of research support: OFFICILAB, CAPES, CPE-1585 Swainsonine measurement in milk of cows poisoned

the lectin histochemistry analysis of a nervous system and was milled, mixed with commercial food and served to the lar and epithelial cells gave positive reactions to Triticum vulgaris, succinylated Triticum vulgaris, Lens culinares, Concanavalia ensiformis, Pisum sativum, Ricinus communis, Arachis hypogaea and Phaseolus vulgaris erythroagglutinin. The positive reactions to these lectins indicates acetyl-neuraminic acid. Conclusions: It is concluded that I.

Mendonça, F.S.3 1. Faculdade de Medicina Veterinária, Universidade de Cuiabá, Brazil 2. Poisonous Plant Research Laboratory, United States Department of Agriculture, USA 3. Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Brazil study aimed to quantify swainsonine in milk of cattle poiMethodology: One adult cow naturally poisoned and two lactating adult cows, and their suckling calves of 2 months were used in the experiments. The detection and quantitation of the swainsonine in milk were accomplished using reversed phase highperformance liquid chromatography. Results: The lesions on poisoned animals were consistent ease. The average concentration of swainsonine in milk

Keywords: Plant poisoning; storage disease; lectin histochemistry CPE-1589 Investigation of DNA from Mycobacterium leprae present at collections of water in the municipalities of Juazeiro and Crato, Northeast Brazil Macedo, M. L. B.¹; Holanda, M.V.¹; Oliveira, T. E. S.¹; 1. Laboratory of Mycobacteria, School of Medicine, Federal University of Ceará, Brazil 2. Department of Geology, Federal University of Ceará, Brazil Introduction: Leprosy is a chronic infectious disease genetic factors, nutritional status, BCG vaccination and source of infection, such as water, soil and animals. The -

swainsonine in the milk. However, under these experimental conditions, the concentration of this alkaloid is not

sosomal storage disease in calves. keywords: cattle, poisonous plant, lysosomal storage disease, alkaloids

M.leprae present at samples of water reservoirs in endemic areas in the municipalities of Juazeiro do Norte and Crato, from nine water tanks, totaling 45 samples. The samples were collected from ponds, streams, rivers and recreational resorts. DNA of M. leprae was extracted with PowerWater formed using “in-house technical”. The product consisted

CPE-1585a Lectin-histochemichal analysis of lysosomal storage disease induced by Ipomoea verbascoidea (Convolvulaceae) in goats Norte and two from Crato. The low positivity may have Correa, F3; Colodel, E.M.4 1. Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Brazil 2. Faculdade de Medicina Veterinária, Universidade Federal do Rio Grande do Sul, Brazil 3. Hospital Veterinário, CSTR, Universidade Federal de Campina Grande, Brazil 4. Departamento de Clínica Médica Veterinária, Universidade Federal de Mato Grosso, Brazil Background: Lysosomal storage diseases result from an jectives: The aim of this paper is to report the results of 70

Conclusion: This study indicates the presence of nucleic acids from M. leprae in these collections of water. Therefore, these reservoirs may represent a source of infection in community. Keywords: environmental water; Mycobacterium leprae; DNA CPE-1604 Immunohistochemistry analysis of the Ciclooxygenase-1 in wound healing submitted to different treatments Martins Neto, V.1; Lopes, C.F.B. 3; Santos, H.B. 2; Thomé, 1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ,

2011 5 (2)

Brazil 2. Laboratório de Processamento de Tecidos – LAPROTEC, CCO/UFSJ, Brazil 3. Curso de Bioquímica – CCO/UFSJ, Brazil Background: The cyclooxygenase (COX-1 and COX-2) acts in the prostaglandins synthesis, molecules that play skin repair. Conventional treatment with nonsteroidal anti-

convulsion.Results: The doses chosen regarding the Artemisia vulgaris had a latency dilated after the administration of pentylenetetrazol when compared to the diazepam higher than the standard.Conclusion: The methanolic extract suggests an anti-convulsive action due to the fact

action of medicinal plants, commonly used to treat skin ferent treatments, associating the expression of cyclooxygenase with the cicatrization process. Methodology: After injury, a total of seven animals per group were treated throughout nine days with water (G1), plant extracts of Plantago sp (G2) and Siparuma sp (G3), and collagenase ointment (G4). Samples of tissue from the wound area ted to immunohistochemistry for COX-1. Analyses were performed using an optical microscope and the Axio Vision software 4.8 Rel. Results: The immunolocalization of COX-1 was predominant in the cytoplasm of epithelial cells in G1, G2 and G3. In these groups there was diffuse

searches of new anti-convulsive drugs. Keywords: Artemisia vulgaris; Anti-convulsing.

two cell layers. In G4 the immunostaining occurred in con-

System. Continuing Education (CE) function is essential

the groups treated. Conclusions: The results suggest that the treatments did not differ in stimulating the synthesis of Cox-1 in the skin repair. Therefore, it is necessary to continue the investigation of the expression of Cox-1 and Keywords: Cox-1; cicatrization; immunohistochemistry. CPE-1605 Anticonvulsant Evaluation of Artemisia vulgaris using diazepam as standard and pentylenetetrazol and convulsing drugs such as lidocaine

CPE-1606 Family Risk Scale as a tool to train and upgrade of the CHAs of the Family’s Health Strategy in the Wasteland rural city Silva, T.H.L.1; Medeiros, J.H.1; Silva, D.M.1; Silva, D.C.A.1; R.T.C.A1; Silva, L.G.A.1 ; Santana, P.R.1 1. Federal Universtiy of Pernambuco, Brazil / Victoria Academic Center- UFPE / CAV, Brazil 2.Federal Universtiy of Pernambuco, Brazil Background: The Family Health Strategy (FHS) was de-

trainees, to encourage them to work through update tools and methodologies that meet these principles. The Community Health Agents (CHA) is part of the FHS, with an important role in upgrading local health indicators. The records of `Form A` (record of the family and their peculiarities). This scale is a guiding instrument for prioritithese datas is part of the CHA`s function, even the data sional to update Form A. Methodology: It was requested of Form A. Was chosen randomly one of the CHA and

M. E. R.¹ ; Silva, A. R. ¹; Soares, P. H. S.¹ ; Alves, J. O.¹ ; Farias, F. C. V. M.¹ ; Almeida, E. R.¹ 1.Laboratory of Psychopharmacology and psychoactive drugs and their toxicology - Federal University of Pernambuco, Brazil Introduction: The genus Artemisia (Asteraceae, Anthemid-

high economic value in some regions, such as food, ornamental plant, as fodder in the African steppes; in alternative medicine it has analgesic, antispasmodic, anticonvulsant, tonic, sedative, digestive, anthelmintic and regulating menstruation. Goal: This study aims to evaluate the anti-convulsing potential of the Artemisia vulgaris through a methanolic exSwiss mice were used. They were divided in three groups for each compulsive drug, each group had six animals usvulsing factor an extract of the dosage was given to its group and after a 60 minutes period the animals received the effects of the convulsive drugs chosen (pentylenetet-

constant updating of Form A. The information generated The reference choosen in this work was the FRS proposed patory, demonstrated commitment to handling the FRS.

essential to seek innovative ways to respond the challenges of an EC. Keywords: Continuing Education, Public Health, Family Health Program. CPE-1606a Recurrent urinary tract infection due to inappropriate use of antibiotic by health professionals: clinical case report Medeiros, R.T.C.A.¹; Soares, N.S.¹; Damasceno, T.Q.¹; Silva, D.M.1; Silva, D.C.A.1; Lira, M.C.C.1 1. Federal Universtiy of Pernambuco, Brazil/ Centro Acadêmico de Vitória – UFPE/CAV, Brasil 71

Experimental Pathology and Health Sciences

in the urinary system. It affects individuals of any age,

patients admitted to a university hospital in Recife. Methodology: A convenience sample was composed of patients admitted to a university hospital in Recife - PE. It is a case study, carried out from simple interview and consultation 33, graduated in pharmacy, was diagnosed with recurrent -

units of Escherichia coli sensitive only to ertapenem and ceftriaxone. The patient had severe side effects to ertapenem, ceftriaxone was administered for 14 days. Conclusions:

not only among laymen. Keywords: Anti-Bacterial Agents, Recurrence, Urinary Tract Infections

shows higher sensitivity, our results demonstrates that primers targeting gyrA region are also very sensitive. Conclusion: These results show that single-step PCR targeting gyrA gene is a potential alternative to detect M. leprae RNA in environmental samples. Keywords: environmental water; gyrA gene; Mycobacterium leprae CPE-1616 Inhibitory effects of natural plant of Family Anacardiaceae on gelatinolitic activity of MMP-9 R.I.M.A.1 1. Laboratório de Biologia Celular e Mutagênese. CCO/UFSJ, Brazil 2. Curso de Medicina. UFMG, Brazil 3. Molecular Oncology Research - Barretos Cancer Hospita , Brazil Background: In the cancer, many of the target genes that and late stages of disease and are required for metastasis and angiogenesis such as matrix metalloproteases (MMPs) that promote angiogenesis and invasive growth of cancer fractionated extracts of a plant of the Family Anacardiaceae. Methodology: The ethyl acetate fraction, partition from the crude extract, was eluted in the chromatographic column and had their points grouped according to the into solutions with the different extracts. To stimulate the proteolytic activity of gelatinases, a portion of the gel was

CPE-1614 Comparison of two different PCR methods based on RLEP and gyrA sequences for detection of Mycobacterium leprae in water environmental samples Oliveira, T. E. S.¹; Holanda, M.V.¹; Macedo, M. L. B.¹;

-

1. Laboratory of Mycobacteria, School of Medicine, Federal University of Ceará, Brazil 2. Department of Geology, Federal University of Ceará, Brazil

-

samples with low amount of DNA. This region is found repeated 28 times in the genome. However, the high sensisequences in environmental water samples. Methodology: from Juazeiro do Norte and Crato municipalites, Northkept at 4C until processed. Five different samples were collected from each point of collection, resulting in total 45 samples. Total RNA was extracted using PowerWater RNA

-

Keywords: gelatinases; plant extract, Anacardiaceae CPE-1618 Evaluation of antitumor activity in vivo Barbatic acid isolated from Cladonia salzmanii (lichen) Neto, M.P.C.1,2; Rocha, T.A.1; Andrade, C.A.S.1,3; Santos, N.P.1,2; Silva, N.H.2; Falcão, E.P.S.1,2 1. Centro Acadêmico de Vitória- CAV/UFPE, Brazil 2. Laboratório de Química de Produtos Naturais, Departamento de Bioquímica – UFPE, Brazil 3. Laboratório de Tecnologia em Biomateriais, Centro Acadêmico de Vitória – CAV/UFPE, Brazil Pharmaceutical agents from natural products have occupied a prominent position in the treatment of pathol-

RLEP and gyrA gene sequences respectively, were visual-

pounds produced is considered exclusive of mycota li-

72

2011 5 (2)

pancreatic hormones, anterior pituitary and adrenal is considered pathology of a chronic nature and rolling, setting used as the experimental Sarcoma 180 tumor. The animals and dental treatment. Periodontal disease is a chronic vical dislocation and organs and tumors were removed and

prevalence levels, and the second leading cause of dental disease in the human population around the world. A

compared with the literature, in focus the fumarprotocetrárico acid isolated which showed an LD50 of around an activator receptor it phosphorylated insulin, causing insulin can no longer activate these receptors, leading to the -

Keywords: Antitumor activity, Barbatic acid, lichen CPE-1620 Alpha-Tocopherol Supplementation Changes The Camargo, Vânia B.; Santos, Amanda M.; Schiavotelo, Na-

in healing, vascular disease, dyslipidemia, among others. Such complications have systemic repercussions, and how

Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, Brasil -

of systemic health.

risk due to its capacity to decrease LDL oxidation, favor arterial vasodilatation and modulate the activity of diverse

-

CPE-1624a Chemiluminescent Detection Of Galectin-3 In Tumoral Tissue From Breast Filho Magalhães, S. D. 1; Lima R. E. 1; Neto Silva, S. T. 1; Silva, R. B. 1; Patu,V.J.R.M. 1 1. Faculdade ASCES mune system and cancer. Immunohistochemical meth-

treated not ovariectomized (T-NO), untreated ovariectoly, every two days, or only the vehicle (sterile peanut oil, 0.2 rum concentration of Triglycerides (TG), Total Cholesterol (TC) and HDL-c using commercial kits. Results: It was

method. gated to acridinium ester (AE) and the complex formed tected. and normal tissues than in IDC). These values showed to these values were dramatically reduced when the tissue

year and at least 72 h, respectively. clusions: alpha-tocopherol supplementation can prevent

in tissue. Breast tissues were used as a model.

drop in HDL-c and rise in not-HDL-c). Keywords: alpha-tocopherol; ovariectomized rats; CPE-1624 Periodontal Disease As A Risk Factor For Diabetes Mellitus Filho Magalhães, S.D.1; Araújo, R.V. S1 1. Faculdade ASCES, Caruaru, Brasil;

CPE-1631 Clinical study, diagnosis and epidemiology of onychomycosis cases reported in the Department of Medical Mycology UFPE Targino Silva, S.1; Lacerda Filho, A.M.1 1. Laboratório de Micologia Médica do Departamento de Micologia da Univesidade Federal de Pernambuco – UFPE, Brazil Background: Onychomycosis is a fungal infection of the nail caused dermatophytes, yeasts or non-dermatophytic. They appear in high frequency, although its clinical and 73

Experimental Pathology and Health Sciences

-

TCNM.

inappropriately. It is crucial the acquisition of clinical samCPE-1634 appropriate procedures for collecting and epidemiological survey of patients treated at the Department of Medical servation of clinical features of lesions that present in the -

Vegetables Show DNA Protector Effect Even Contaminated With Pesticides: A Study By Comet Assay Dias, S. A.2; Silva, E. J.1; Freitas, E. M. S.2; Adam, M. L.2; And Chagas, C. A.1 1. Postgraduate program in Human Health and Environment, Academic Center of Vitória - Federal University of Pernambuco, Brazil 2. Academic Center of Vitória - Federal University of Pernambuco, Brazil

mainly species of Candida; Filamentous fungi were isolated in 22 cases, with emphasis on species of Fusarium and Trichophyton. Of the patients 60 were women and 11 men, with ages ranging from 15 to 80 years. Conclusions:

teen Wistar rats were divided in three groups and were -

treatments for onychomycosis, since this is a disease of Keywords: Diagnosis; Fungi; Onychomycosis.

-

CPE-1633 Histochemistry Analyses of Sialic Acid Residues In Non-Melanome Tumours Using SNA Ferreira, S.A.1; Beltrão, E.I.C.1,2 1. Keizo Asami Immunopathology Laboratory (LIKA), Federal University of Pernambuco (UFPE), Brazil. 2. Biochemistry Department, Biological Science Centre, Federal University of Pernambuco, Brazil. (NMCT) are the most incidence malignant neoplasia in ent types of tumours associated to stages of progression and development of tumour cell. OBJECTIVES: This socellular carcinoma (BCC) and spinocellular carcinoma -

out the Comet Assay. Results: Many different pesticides Daily Intake – ADI. Both parameters of analysis “Index of Damage” - ID - and “Frequence of Damage” - FD showed that the Group 1 had a higher damage level than cant difference was found in other comparisons. The difpromoted an antigenotoxic effect, protecting the animals clusions: Despite the presence of pesticides, the ingestion experimental design carried out. Keywords: Agrochemicals; DNA Damage; Antigenotoxicity. CPE-1634a Mutagenicity In Agriculture Workers Exposed To

Biotin-Streptavidin-Peroxidase system was used to lectin ing reagent. Haematoxylin was used for counter staining and results evaluated using light microscopy. Cell staining was evaluated according Dornelas (2009): (+) positive for cells; (-) negative. Three random areas of tissues were ana-

BCC and SCC presented distinct patterns of sialic acid residues expression in their cell surface glycoconjugates. Results indicate that SNA can differentially recognize auxiliary tool in histochemistry. Key-words: Sambucus nigra agglutinin, sialic acid, 74

Of Personal Protection Equipments In Micronuclei Frequency Dias, S. A.1; Silva, E. J.2; Anjos, M. D. A1; Lima, C. D. S¹; Freita, E. M. S.1; Adam, M. L.1; And Chagas, C. A.2 1. Academic Center of Vitória - Federal University of Pernambuco, Brazil 2. Postgraduate Program in Human Health and Environment, Academic Center of Vitória - Federal University of Pernambuco, Brazil tigate the impact of exposure to genotoxins. Agricultural workers are prone to occupational exposure to a variety of pesticides which represents a potential risk to human

2011 5 (2)

the wound healing submitted to different treatments.

the use of Personal Protection Equipments (PPE) in micronuclei frequency were also evaluated in these workers. Methodology: The study included 54 individuals, 31 farmers and 23 non-exposed controls. The farmers were evaluated in two moments: during pesticide application -

R.I.M.A.1 1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ, Brazil 2. Laboratório de Processamento de Tecidos – LAPROTEC, CCO/UFSJ, Brazil Background: Mast cells have a central role in immediatetype allergy and also related to regulating the cutaneous wound healing. The medicinal plants are used in the treat-

and stained with Giemsa. The frequency of micronucleated cells was performed in 1000 leukocytes per individual. of micronucleated cells when compared to control group (36.77±14.42 verses 11.60±7.55). The Post-PPE showed Whitney) in when compared with the exposed group. Such factors as alcohol intake, time of exposure, age and sex had no effect on the frequency of micronucleated cells in all experimental groups (P>0.05). However, smoking to an increase of DNA damage in farmers. The use of PPE reduced the exposure to pesticides and consequently the damage was repaired. Smoking was the only paramenhance the DNA damage. Keywords: Agrochemicals; Cytogenetic Damage; Biomonitoring CPE-1635 Adrenal Microanatomy Alteration Of Pinealectomized Rats At The Age Of 39 Days Dias, S. M. S.1; Sousa, I. D. D.1; Santos, K. R. P.1 1. Universidade Federal de Pernambuco, Brasil

to different treatment. Methodology: After injury, a total of seven animals per group were treated throughout nine days with water (G1), plant extracts of Plantago sp (G2) and Siparuna sp (G3), and collagenase (G4). Samples logical sections were acquired using an optical microscope and the Axio Vision software 4.8 Rel. Statistical analysis were expressed as means ± SEM. Results: In relation to epithelialization until nine day, the G1, G2 and G3 groups presented skin repair, while in G4 was not occurred. The mast cells with normal and degranulated morphology were recorded surrounding the wounds in all treatments. The quantitative analyses of the mast cells population showed the follow values: G1 (range 0.0 – 46.0; mean 17.7 ± 1.3), G2 (range 0.0 – 45.0; mean 18.5 ± 1.3), G3 (range 3.0 – 42.0; mean 22.0 ± 1.5) and G4 (range 0.0 – 43.0; mean and the other treated with collagenase. Conclusions: The

the pineal gland on the adrenal gland. Studies reported the presence of melatonin receptors in the adrenal gland,

healing skin. Keywords: mast cells, Plantago and Siparuna extracts, skin repair.

in the adrenal glands through the pinealectomy such as: meaningful increase of the vascularisation, decrease of the mone (ACTH), hypertrophy of the cortex layer, among

CPE-1637 Cytotoxicity of the extract of the leaves of microemulsion Schinopsis brasiliensis Engl. front macrophages cells Castro, R.H.A.1; Batista, D.C.A.1; Coutinho, F.N.1; Saraiva, A.M.1; Silveira, W.L.3; Silva, T.G.2; Egito, E.S. T.3; Psiciottano, M.N.C.1 1. Laboratory of Microbiological Analysis, Depto. Pharmaceutical Sciences, UFPE, Brazil 2. Laboratory of bioassays for Research Department-Drug. Antibiotics - UFPE, Brazil 3. Dispersed Systems Laboratory. Faculty of Pharmacy - UFRN, Brazil

the adrenal microanatomy alterations of pinealectomized rats at the age of 39 days. Methodology: 20 Wistar rats were used. They were separated into two groups: pinealectomized Sham rats and pinealectomized rats. At the end of a-seven-month post surgery period the rats were anesthetized, euthanatized, and necropsied to remove the adrenal were processed, cut at 3 micro centimetres thick, undermicroscopy. Results: Results showed a meaningful increase of the adrenal gland vascularisation. The zona fasciculate

-

-

evaluated the cytotoxicity of microemulsion extraction

ciculate. Keywords: Adrenal. Morphology. Pineal Gland.

front macrophage lineage cells. Methodology: The mac-

CPE-1636 Quantitative analyses of mast cells population during

The cells were resuspended in RPMI medium supplement75

Experimental Pathology and Health Sciences

tant was discarded and then the methanol extract of leaves (MTT). Again taken the greenhouse for 2 hours (37 0C) and then the culture medium along with the excess MTT was aspirated and added dimethylsulfoxide wells. The opti-

low cytotoxicity against macrophage cells, encouraging result, although in vivo toxicological studies are needed to reach further conclusions. Keywords: Schinopsis brasiliensis Engl.; microemulsion, Cytotoxicity. CPE-1637a Cytotoxicity of the extract of the leaves of microemulsion Schinopsis brasiliensis Engl. front lineage leukemic cells (K563) Castro, R.H.A.1; Batista, D.C.A.1; Coutinho, F.N.1; Saraiva, A.M.1; Silveira, W.L.3; Silva, T.G.2; Egito, E.S. T.3; Pisciottano, M.N.C.1 1. Laboratory of Microbiological Analysis, Depto. Pharmaceutical Sciences, UFPE, Brazil 2. Laboratory of bioassays for Research Department - Drug. Antibiotics - UFPE, Brazil 3. Dispersed Systems Laboratory. Faculty of Pharmacy - UFRN, Brazil -

Brazil: an analysis of data obtained by PNDA (19982008) Nascimento, R.A.F.1; Almeida, R.N.1; Canadá, M.N.S.1; Carneiro, R.S.1; Santos, A.J.H.1; Sousa, A.B.1 1. Faculdade Santa Emília de Rodat Background: In Brazil, the National Household Sample jectives: This study aims to characterize the chronically ill from the National Household Survey (health supplement) of the years 1998, 2003 and 2008. Methodology: Data were individuals who reported having at least one chronic disease to PNDA. Data were collected in the system DAof patients with chronic diseases were selected the folcome per capita and coverage of health insurance. Results: The PNDA recorded that DC prevalence in the population resident (PR) in Brazil in 1998, 2003 and 2008 were

2008 the percentage of DC was reported higher among those who had an income of two minimum wages or more years a higher growth in the population of DC than in population of DC for the implementation of more effecKeywords: Chronic diseases; Prevalence.

study evaluated the cytotoxicity of microemulsion extracEngl. front lineage leukemic cells (K563). Methodology: Cells (K563) were plated in suspension (density of 0.3 x 25 and 50 µg.mL-1, the positive control (etoposide, 1.25 to 25 µg.mL-1) and negative control (dimethyl sulfoxide) pernatant discarded. Each cavity received 200µL of MTT

was discarded, and the pellet was resuspended in 150µL and forward the lineage leukemia (K563) of 29.7 and 17.04 µg.mL-1, respectively. The control reached an IC50 of 4.4 µg.mL-1. Conclusions: We found less cytotoxic action Expenetration of the micelles of the microemulsion-lineage totoxicity against leukemic strain tested, requiring larger studies to further conclusions we draw. Keywords: Schinopsis brasiliensis; microemulsion, Cytotoxicity (K563). CPE-1638d Characterization of patients with chronic diseases in 76

CPE-1639 Prophylactic Effect Of Administration Of The Ascaris Summ Antigen Extracts On Experimental Liver Injury Mediated Immune Cells N.T2; Souza, V.M.O1. 1. Department of Immunology, Laboratório de Imunopatologia Keizo Asami - LIKA/UFPE, Brazil. 2. Department of Pathology, LIKA/UFPE, Brazil. cells are essential in the pathogenesis of tissue injury, as Ascaris suum adult worms (Asc) has proteins with regulatory activities that can modulate the immune response to antigens homologous or heterologous in the host. However, there are few studies of the immunoregulatory action of this extract in a model of autoimmune hypersensitivity -

allocated in a control group (which received PBS i.p. and 30 minutes later, PBS i.v.), an AIH (received 300µL of PBS i.v.). Blood were collected 8, 24 hours and 7 days after administration of treatment to measure transaminases (ALT of animals through ELISA. Results: Transaminases levels

2011 5 (2)

AST) in relation to AIH+Asc. The total Ig levels were sigmorphometrically quantify the immunostain of galectin-3 not meaningful. After 7 days of administration of ConA,

and invasive ductal carcinoma (IDC) in humans. Immunohistochemistry was developed using monoclonal anti-

in the control group. Conclusions: The results showed that hepatocellular damage. Keywords: Ascaris suum; Immunomodulation; Autoimmune Hepatitis. CPE-1649 Men can become infertile after infection with Chlamydia trachomatis? A focus on semen parameters. Florencio de Oliveira, T.H.1; Souza Feitosa, A.R.1; Torres Silva, M.J.1; Oliveira Moraes, R.F.1; Vázquez, C.S.2 1. Faculdade ASCES, Brazil 2. Department of Functional Biology and Physical Anthropology – University of Valencia, Spain

(DAB) and hydrogen peroxide. Image analysis was carried out using a workstation consisting of a standard light microscope equipped with a digitalizing camera connected to a desktop personal computer. Image storage and retrieval was managed using the OPTIMAS® software system. Results:The immunohistochemical staining studies dem-

ing to have children, can not generate them, and that the

-

the factors harmful to reproduction is the pathology of in-

Keywords: Immunohistochemistry, tumors, breast

and quality sperm, causing a change in semen parameters, -

CPE-1682 Synthesis, Structural Characterization and Evaluation Antinociceptive Activity of the 2 [(benzylidene)-amino] -4,5,6,7-tetra-4H-benzo [b]thiophene-3-carbonitrile

with alterations present in the semen seminal aspects that serve as a diagnosis for male infertility. Methodology: The study was conducted through literature review searches Results: According to the articles analyzed, infection with Chlamydia trachomatis is the leading cause of epididymitis, and other infections that directly affect the cells tion of the seminiferous canals, resulting in the call trace

A.R.S,2; Pereira, C.K.S.2; Salvadori, M.G.S.S2; Assis, T.S.2; Almeida, R.N. 2; Moura, R.O 1; Mendonça-Junior, F.J.B.1. 1. Laboratório de Síntese e Vetorização de Moléculas, Universidade Estadual da Paraíba, Brazil; 2. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Brazil Background: The compounds thiophene derivates, in great variety of pharmaceutical properties, such as anti-

factor highlighted was the presence of cells involved in immune response, causing agglutination and changing the parameters related to motility. Conclusion: Assuming that

synthesis of new thiophene derivative to evaluate their activity anti-noceptive propriety. Methodology: The syn-

data in relation to semen parameters changed, it is clear . trachomatis, act directly on male fertility, since these aspects in question are of great importance in the success of fertilization. Keywords: Chlamydia trachomatis, infections, infertility CPE-1650 Immunohistochemistry Detection Of Galectin-3 In The Breast Tumoral Tissues Silva, R.B.1; Silva, E.M1; Silva, M.I.C.1; Negreiro, J.S1.; Morais,J.A.S.1; Silva,J.T.1; Silva, R.C.1; Filho, S.D.M.1; Patu, V.J.R.M1; Carvalho-Junior, L.B.2. 1. Faculdade ASCES Caruaru- PE, 2. Pós-Graduado em Ciências Biológicas, do Centro de Ciências Biológicas da Universidade Federal de Pernambuco Background: Immunohistochemistry helps pathologists in

25-35 grams, 3 months of age were divided into groups with eight animals. The control group received distilled water and tween 20, 4 experimental groups received the The compounds were characterized, their structures were inal constrictions when compared with the control group. 77

Experimental Pathology and Health Sciences

response to the administered dose. Conclusions: Was synthesized a new derivative 2-amino thiophene with Keywords: Thiophene Derivatives, anti-nociceptive, Gewald reaction CPE-1683 Behaviour of surgical site infection in splenectomy: Results of a two-year retrospective study tos I.T.S1, Xavier A.T1, Oliveira, RAA1; Muniz, MLC1, Costa, AB1 1. Universidade Federal de Pernambuco-Centro Acadêmico de Vitória, Brasil for the mortality of postsurgical patients, where the risk of sepsis is sixty times higher after a splenectomy procedure.

outside the hospital. Seeing the CAH as a rescuer, he or cy clinics and in medical and traumatic emergencies which which occurred during the “Rondon King of the Bay Project” in 2010. Methodology: The training took place in the city of Cedro-PE, with a total workload of 15 hours of input, from the 20th to the 23th of July 2010, through theoretical and practical lessons, using audio-visual material, projections, makeup simulation of complications, speof the undergraduate nursing Victoria Academic Center, were included in the programe. Results: Previous knowledge of the procedures was scarce and many profession-

years 2009 and 2010. Methodology: This is a retrospec-

results showed that the training was extremely satisfying and the CHAs demonstrated that they had assimilated the theoretical content with a good practical performance in the simulations as well. Conclusions: Training courses for

to 39 patient records. Results: A higher incidence of SSI -

Keywords: Health education, community health, basic life support.

and mostly hepatosplenic schistosomiasis were operated

CPE-1686 Liver changes in diabetic rats and alcoholics

not perform hair removal. There is no record of preoperathe surgeon. Conclusion: What we try to prove with the release of our results is that the ISC splenectomies per-

1. Universidade Federal do Vale do São Francisco, Brasil 2. Colegiado de Medicina - Universidade Federal do Vale do São Francisco, Brasil 3. Colegiado de Ciências Farmacêuticas – Universidade Federal do Vale do São Francisco, Brasil Background: Alcoholism is an important inducer of pathophysiology of liver tissue. These changes are initi-

showing that infection control is done with political decihospital infection. KEYWORDS: splenectomy, infection; wound infection CPE-1683a Initial Care In Urgencies And Medical And Traumatic Emergencies: Training Course Given To Community Health Agents In The Interior Of Pernambuco/ Brazil Galindo Neto, NM¹; Oliveira, RAA1; Muniz, MLC1, Sanquerque TT1, Silva ACA2, Santos LRC2 1Nursing Department. Federal University of Pernambuco, Vitória Academic Center, Brazil. 2 Faculdade de Enfermagem Nossa Senhora das Graças-FENSG Universidade de Pernambuco- UPE Background: The community health agents (CHA) are a als and need trainings on different areas. In emergency situations, the evaluation of the victim and her care should -

steatosis is not common. Steatosis is the most common histologic changes, consequent to the excessive deposition of triglycerides in the liver, occurring most commonly in alcoholic steatosis, which is more frequent amongalcoholic liver disease. Methodology: Were used twenty rats (Rattus

concentrations of alcohol solution. The animals had the were stained with hematoxylin and eosin. Results: Focal -

Keywords: diabetes; alcoholism; liver. 78

2011 5 (2)

CPE-1686a Histological analysis of kidneys of diabetic rats alcoholics

1. Universidade Federal do Vale do São Francisco, Brasil 2. Colegiado de Medicina - Universidade Federal do Vale do São Francisco, Brasil 3. Colegiado de Ciências Farmacêuticas – Universidade Federal do Vale do São Francisco, Brasil ure is the second leading cause of death in this disease. Another factor directly associated with kidney failure is the

divided in groups: untreated (control) and treated with animals were euthanized and the organs (livers and small intestines) were collected for histological processing and posterior morphologic analysis. Results: In the untreated

-

etiologies in pathological processes of renal tissue, this Methodology: In this experiment, were used twenty rats application of intra-peritoneal alloxan at a concentration

of FZ-4 was effective in protecting the intestinal mucosa and the hepatic parenchyma of the granulomatous reaction in mice infected with S. mansoni. Keywords: FZ-4; Schistosoma mansoni; Morphological analysis.

weeks with increasing concentrations of alcohol solution. The animals had their right kidney and other organs colpared histological sections stained with hematoxylin and eosin. Results: At histological analysis, there was extensive were potentiated in the group alcoholic. Conclusions: Lesions that affect the glomeruli are the most common re-

animals only, the severity was due to enhanced association of pathologies. Keywords: diabetes; alcoholism; kidney. CPE-1707 Effect of FZ4 in Schistosoma mansoni infected mice: morphological analysis of livers and small intestines Dias, M.R.P. 1; Bassoli, A.C.D.G.1, Silva, L.L.S.1; Silva, E.C.1; Pitta, I.R.2; Galdino, S.L.2; Medeiros, P.L.1,2 1. Departament of Histology and Embryology CCB - UFPE, Brazil. 2. Posgraduate Program in Therapeutic Innovation CCB - UFPE, Brazil. Background: According to World Health Organization (WHO), more than 200 million people in the world are in situation of infection risk. In Brazil, the illness already reached the majority of the states, mainly, of the North-

CPE - 1715 Use Of Ulex Europaeus Agglutinin I Conjugated To Acridinium Ester As A Chemiluminescent Tool For Of L-Fucose Recognition In Cutaneous Tumours Bezerra, M.F1, Lima, L.R.A1, Almeida, S.M.V1, Cavalcanti, C. L. B1, Junior, L.B.C1,2, Beltão, E.I.C1,2 1. Laboratório de Imunopatologia Keizo Asami (LIKA), Brazil; 2. Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Brazil. tions are related to tumour proliferation, metastasis and evasion of immune system. Lectin histochemistry is a technique used for the investigation of those glycophenotype changes. Chemiluminescence assay is a promising and effective technique for quantitative analysis of proteins, gated to Acridinium Ester (AE) to quantify the L-fucose maglutinatin activity and chemiluminescence (expressed as

gate, separately, and chemiluminescence assayed. Results:

-

promising tool for quantitative assessment of L-fucose tivity of conventional histochemistry using haematoxylin and eosin in skin diagnosis and provides a target for cancer therapy or diagnosis. Keywords: chemiluminescence, histochemistry, lectins.

morphology of livers and small intestines of the infected males, with 45 days of age and weighing of 25-30g had

CPE - 1722 VDRL test in the diagnosis of syphilis. Evaluation of results of the secretary of health Sapé – PB -

this disease. In searching for molecules with antiparasitic

79

Experimental Pathology and Health Sciences

1. College of Santa Emilia Rodat, Brazil. 2. State University of Paraiba, Brazil. Introduction: Syphilis is a systemic infectious disease with clinical and pathological manifestations. The diagnosis is tives: This study aims to analyze the results of tests non tory) of patients seen in primary care unit. Methodology:

lous district of the city. The most frequent clinical manifestations were keratoconjunctivitis and onychogryphosis

demonstrated an increase in the cases of CVL in the period and the most endemic area was concentrated in the medium and lower-medium social level district. The clinical analysis in infected dogs showed that the most frequent Keywords: anthropozoonosis; leishmaniasis, urban reservoir

uses the extracted tissue antigens such as cardiolipin, a liplecithin and cholesterol, form a serologically active antigen disease, can occur so false-positive results due to disease tions among others. (VAZ et al.1996; LIMA et al.1999, -

results demonstrate the presence of syphilis in our day and the importance of this test especially during pregnancy, in order to avoid contamination placenta. Conclusion: Al-

CPE - 1723 Analysis of the healing time of skin wound on mice submitted to treatment with plant extracts Pereira, M.N.1; Guimarães B.F.1; Longatti, T.R. 1; Oliveira, C.D. 3; Moreira, G.A. 1; Oliveira, R.J.4, Santos, H.B. 2; Ri1. Laboratório de Biologia Celular e Mutagênese - LabCcel/CCO/ UFSJ, Brazil 2. Laboratório de Processamento de Tecidos - LAPROTEC/ CCO/UFSJ, Brazil 3. Campus Centro Oeste - Universidade Federal de São João Del Rei, Brazil 4. Molecular Oncology Research - Barretos Cancer Hospital, Brazil. peutic value is associated with many cultures, especially in the medicinal effectiveness or the toxicity of analyzed ex-

with clinical assessment, early diagnosis, and therapeutic Keywords: Syphilis, VDRL CPE – 1722a Clinical and epidemiological study of dogs with Visceral Leishmaniasis in the city of João Pessoa, Paraíba, Brazil 1. State University of Paraiba, Brazil. 2. Zoonoses Control Centre, João Pessoa. Brasil Introduction: The Canine Visceral Leishmaniasis (CVL) is serious systemic zoonoses with chronic and slow progress agent is the protozoan Leishmania (Leishmania) chagasi, link in the transmission cycle of CVL, through of the Luttreated at Zoonoses Control Center in the city of João Pessoa-PB and to identify the most common clinical alterations in the symptomatic animals. Methods: The data were

the analysis of the time of healing in injuries induced and treated with vegetal extracts. Methodology. The Plantago topical treatment was with watery solution of two extracts (G2 and G3), controlled group (water) and as positive control, an ointment especially used in postoperative wounds. uted in 4 treatments with 14 animals each. Closing wounds time and injuries appearance were evaluated during 21 days. Results. The control group (water) and G2 presented healing times smaller. The group treated with the ointment presented the worst performance. Macroscopically, it was 10th and 15th day after injuries. Moreover, all the animals of these two groups had completed the healing of the Plantago (G2) presented minor time of healing in relation

in the period of 2005 to 2009. Twelve dogs seropositive the clinical characteristics analyzed. The technique applied

statistical difference when compared to the group treated with the ointment. Keywords: Wound healing. Extracts of plants. Mice CPE - 1725 Development of an electrochemical biosensor based on gold nanoparticle-polyaniline composite for tuberculosis detection Maurilia Palmeira da Costa1, César A. S. de Andrade1,

cases). The data suggest that the most endemic districts were in the south area, with Bairro dos Bancários, Valen80

1- Centro Acadêmico de Vitória, UFPE, Brasil; 2- Centro de Pesquisas Ageu Magalhães, Recife, Brasil; 3- Departamento de Bio-

2011 5 (2)

química, Centro de Ciências Biológicas, UFPE, Brasil.

CPE - 1726 Detection of viable Mycobacterium leprae in water samples from Juazeiro do Norte and Crato, Northeast Brazil Holanda, M.V.¹; Oliveira, T. E. S.¹; Macedo, M. L. B.¹;

The gold nanoparticles (AuNps) facilitate the transfer of sis (MTB) under the surface of gold electrode (Au) to analyze their interaction of AuPANi-MTB system and DNA spectroscopy (EIS) was performed in a frequency range of 100 KHz to 100MHz and cyclic voltammetry (CV) with a voltage of -0.2 to 0.7 V, in the presence of potassium ferricyanide 10mM as redox couple. The EIS and CV measurements demonstrated decreased the redox reactions of MTB system demonstrated an additional decrease in real

1. Laboratory of Mycobacteria, School of Medicine, Federal University of Ceará, Brazil 2. Department of Geology, Federal University of Ceará, Brazil Background: Leprosy is a chronic dermatological and

tal water samples in endemic areas of Juazeiro do Norte and Crato, Northeast Brazil. Methodology: Water samples from rivers, lakes and damns were collected in sterile dark

with AuPANI-MTB and MTB-AuPANI-TB2, a decrease -

in total 45 water samples. The water samples were processed for total RNA extraction using PowerWater RNA Isolation kit (MoBio). cDNA was synthesized using the OneStep RT-PCR kit (Qiagen) and primers targeting gyrA

Keywords; biosensor, tuberculosis, impedance, bioelectrochemistry CPE – 1725a Development of an electrochemical biosensor based on gold nanoparticle-polyaniline composite for tuberculosis detection Costa,M P1; Andrade, C A S1; Melo F L2; Oliveira, M D L3 1- Centro Acadêmico de Vitória, UFPE, Brasil; 2- Centro de Pesquisas Ageu Magalhães, Recife, Brasil; 3- Departamento de Bioquímica, Centro de Ciências Biológicas, UFPE, Brasil.

ervoir all samples were positive. Conclusion: The recovery of RNA sequences from an RNA template using RT-PCR implies that the source organisms were live at the time of ing a role in the continued transmission of the disease. Keywords: environmental water; Mycobacterium leprae; viable bacilli CPE - 1727

The gold nanoparticles (AuNps) facilitate the transfer of sis (MTB) under the surface of gold electrode (Au) to analyze their interaction of AuPANi-MTB system and DNA spectroscopy (EIS) was performed in a frequency range of 100 KHz to 100MHz and cyclic voltammetry (CV) with a voltage of -0.2 to 0.7 V, in the presence of potassium ferricyanide 10mM as redox couple. The EIS and CV measurements demonstrated decreased the redox reactions of MTB system demonstrated an additional decrease in real

abattoirs at Pernambuco Zona da Mata Norte Pereira, M.F.1 1- Departament of Veterinary Medicina of Federal Rural University of Pernambuco, Brazil that, in some way, prevent or constrain the use of the product or raw material for human consumption. Post-mortem human consumption, verifying the presence of changes in organs and tissues that may indicate the existence of dis-

with AuPANI-MTB and MTB-AuPANI-TB2, a decrease photographed and fragments were collected and placed in Keywords; biosensor, tuberculosis, impedance, bioelectrochemistry 81

Experimental Pathology and Health Sciences

and stained with hematoxylin and eosin (HE). Results: Approximately 60 animals were slaughtered (30 at Carpina animals. Among them, there are eight with swelling of clusions: In slaughterhouses visited, post-mortem changes were mainly found in lymph nodes and lungs. Keywords: Inspection, pathology, cattle. CPE – 1728a

volved different genetic, immunological and environmenfrom the Hospital das clínicas (HC) and the Barao de Lucena Hospital (HBL) with factors such as gender, age,

were selected. The inclusion criteria for CD patients were tion criteria of Lennard-Jones (1989). All patients were

Oral Ulcers Of Rats Treated With Bixa Orellana L. Extract (Annatto, 5,5% Bixin) Souza, M.F.A.M.1. ; Santos, J.A.A.1. ; Silva, E.L.V.1. ; Aguiar Jr, F.C.A.1. 1. Laboratory of Biotechnology and Pharmaceuticals, Academic Center of Vitoria – Federal University of Pernambuco, Brazil Background: The healing of oral ulcers is a complex cal events. Mast cells are connective tissue cells that act host defense and homeostasis. Annatto is an important

-

-

reveal that, in the study area, the CD is more common in men, working-age adults, mixed ethnicity, and presented the shape of stenotic disease. Keywords: Crohn’s disease, epidemiology.

cally induced in rats treated with of Bixa Orellana L extract

CPE - 1732 Evaluation

Of

The

Inclusion

Complex

Of

Models Laranjeira, L.P.M 1; Sitônio, M. M 1; Carvalho, C. R 1; cm diameter wounds were surgically made on the dorsal tongue surface of thirty male wistar rats. The tongue ulrats on the 6th hour, and 1st, 3th, 7th and 14th postoperative days were processed for histology. The histological

A.J.S1; Silva, T.G. 1. 1. Laboratório de Bioensaios para Pesquisa de Fármacos, Departamento de Antibióticos - Universidade Federal de Pernambuco, Brazil 2. Laboratório Tecnologia de Medicamentos, Departamento de Farmácia - Universidade Federal de Pernambuco, Brazil Background: There are in the market several drugs for mon in pharmacotherapy is the use of NSAIDs, which

mast cells in the treated animals was reduced on the1st lapachol, which possess therapeutic properties and toximast cells during the healing process of oral ulcers in rats. Keywords: Mast cells; tongue; Annatto. CPE - 1730 Epidemiological aspects of Crohn’s Disease in Hospitals from Pernambuco, Brazil Santos, L. P.1; Fernandes, W. N.2; Telles, A. M. S.4; Lima, F.E.B.A.5; Martinelli, V. 5; Toscano, M.6; Melo-Junior, M.R.3,4 1. Centro de Ciências da Saúde - UFPE, Brazil. 2. Programa de Pós-graduação em Patologia - UFPE, Brazil. 3. Laboratory of Immunopathology Keizo Asami - LIKA/ UFPE, Brazil. 4. Department of Pathology - UFPE, Brazil. 5. Hospital das Clínicas- UFPE, Brazil. 6. Serviço de coloproctologia do Hospital Barão de Lucena, Brazil. heterogeneous group of diseases wich the last common 82

realized paw edema carrageenan-induced and rheumatoid To edema formation in rats, a 0,1mL carrageenan soluthe right hind paw. The animals were pre-treated (p.o.) 60 measured with a plethysmometer immediately prior to the injection of carrageenan and thereafter at hourly intervals for 5 h. In the arthritis induction, the treatment was On the third day, 0.1 µL FCA was injected in the right hind (mL) during 14 days, using plethysmometer. Results: The the fourth hour (0.35±0.07), when compared with saline control (0.50±0.09). In the rheumatoid arthritis develop-

2011 5 (2)

tion, when compared with the control (1.00±0.03). Con-

ated from nested two-stage PCR of RLEP region and a

rRNA primers respectively. Discussion: The most sensimatory

repeated 28 times in the M. leprae genome. The primers

CPE - 1733

less sensitive. In addition, nested PCR increases the sen-

Leprosy in Fortaleza-Ce Assis, LC 1; Lima, LNGC 1; Marques, LCM 1; Dantas, JCP 1; Kerr, LRS 1; Frota CC¹. Laboratory of Mycobacteria, Medicine School, Federal University of Ceará, Brazil Introduction: Single nucleotide polymorphisms (SNPs)

of DNA from clinical samples. However, reactions using a hinder the interpretation of agarose gel. Conclusion: The of M. leprae from clinical samples with small amount of Keywords: Leprosy, PCR, molecular detection

leprae prevalent genotypes in patients with leprosy in Fortaleza-Ceará Methodology: Nasal samples from 34 leprosy teinase K. The SNPs were divided into two groups, called genotypes 1 or 2 and 3 or 4. Genotyping was performed

CPE - 1735 In silico analysis of the most expressed 14 contigs in a Leishmania chagasi cDNA library from the Northeast Genome Program Lins Marinho, L.A.¹; Oliveira, E.B.¹; Zacarias da Silva, Silva, E.C.¹ 1 – Tissue Culture Lab/Department of Histology and Embryology/CCB-UFPE, Brazil 2 – Genetics Department/CCB - UFPE, Brazil 3 – University Salgado de Oliveira – UNIVERSO, Brazil Background: Visceral leishmaniasis or kala-azar is a endemic zoonosis in the Americas, having Leishmania chagasi as its etiological agent. Its genome comprises ca. 8,500 genes spread among 35 chromosomes. The Northeast transcriptome of this protozoan. The consortium pro-

all samples were male. A similar rate of male and female was seen among the samples that did not amplify. On the other hand, three male had nasal samples with different genotypes. Conclusion: The genotype 1 or 2 of M. leprae was the most prevalent in our study population. Genotype lent in Ethiopia. Keywords: Genotyping, SNP, Mycobacterium leprae. CPE – 1733a Comparison of 16S rRNA and RLEP genomic releprae from nasal clinical samples Assis, LC 1; Marques, LCM 1; Lima, LNGC 1; Dantas, JCP 1; Kerr, LRS 1; Frota CC¹. Laboratory of Mycobacteria, Medicine School, Federal University of Ceará, Brazil petitive element) and 16S rRNA regions are used for molecular detection of M. leprae DNA. OBJECTIVE: To analyze the PCR positivity of the regions 16S rRNA and RLEP in the diagnosis of M. leprae from nasal cliniquence; while samples from 169 patients were analyzed -

most expressed contigs, depicting their functions and chromosome position. Methodology: Sequences from

ac.uk), using the online tools BLASTn e BLASTx. Putative sults: From the 14 contigs studied, one mapped on chromosome 8, another on chromosome 23 and all other on proteins from Leishmania and the other 12 were similar to

presence of rRNAs among the most frequent ESTs is exnot have as assigned function until now. This result opens lism and pathogenicity. Keywords: Leishmania; cDNA library; Genome CPE – 1750 Pernambuco in during the period 2009 to 2011

83

Experimental Pathology and Health Sciences

BGS4; Medeiros, JH5; Lorena Júnior, EJP6; Santos, LS7; of the holder, also makes it mister disclosure of Pap smears 1,2,3,4,5,6,7,8,9 e 10.Department of Nursing. Federal University of Pernambuco, Brazil. disease. Introduction: The exogenous intoxication is the manifes- Keywords: papillomavirus, neoplasms, cytology tation, through signs and symptoms of the harmful effects produced in an organism, a result of interaction with one CPE - 1753 Cytopathology Changes In Women That Made Use Of - Intrauterine Device (Biomaterial) - Ordonho, Kamyla Brasileiro De Lima.¹ ; Araujo, Thereza Cristhinna.¹; Travassos, Karine Dantas.1; Oliveira, Stepha1 - Academic Biomedicine Maurice of Nassau - Brazil 2 - Lecturer in Biomedicine Maurice of Nassau - Brazil

-

-

circumstances, and evolution of intoxication. Results: During this period 10,385 cases were reported, predomi- inexpensive changes in the diagnosis of morpho-cells in the

without sequelae. Conclusion: These indexes suggest the promotion of control measures and health education focusing on the group most at risk, as well as reducing the incidence of self-annihilation. Keywords: exogenous poisoning. Public health. Suicide

an exploratory study of quantitative approach to document the data collection was performed in the compulsory period of Campina Grande - PB. Results: The results were as follows. Of the 48 cytological study showed that the use of

CPE - 1752 Analysis Of The Prevalence Of Human Papillomavi- health policies directed to this area particularly with the presrus Women In The City Of Esperança – Pb ence of family health programs and monitoring of patients Travassos, Karine Dantas1; Oliveira, Stéphanny Sallomé Dias1; Araújo, Thereza Cristhinna1; Sousa, Giovanni Tavares2. 1 – Academic of Biomedicine Maurice of Nassau – Brazil 2 - Student of Biomedicine Maurice of Nassau – Brazil Introduction: The Human Papillomavirus is now considered as one of the diseases that most affect women world-

Keywords: IUD, Papanicolaou smear results CPE – 1755 Prevalence of intraepithelial high-grade injuries in the rectum and anal canal in patients with a history of squamous neoplasia of the lower genital tract

it has strong link with cervical cancer and is one of the R.I.M.A. 1 - 1. Universidade Federal de São João Del Rei. CCO/UFSJ, Brasil of cervical cancer has a strong association with the occurprevalence of women aged 20 to 50 years with results of rence of anal canal cancer. Its early detection allows the preology: To this end we conducted a research of descrip- virus (HPV) anal infection in patients with cervical intrative type with a quantitative approach with a retrospective epithelial neoplasia. documentary. Included were women aged 20 to 50 who had Pap smears, as soon was used as the research instru- four women with cervical intra-epithelial neoplasia, treated surgically or not, without chemotherapy or radiotherapy health department of the city of Esperança - PB (1116 laou. Koilocytosis indication was considered pathognomonic of HPV infection and lesions indicative of anal intraepithelial neoplasia grade I. Results:The average age of patients was 36 years, and aver67 patients had at least CIN I, the highest prevalence was of patients stated they do or have already made use of oral contraceptives. These aspects are risk factors for infection respectively. Conclusions: Considering the results empha- canal HPV infection and AIN grade 1: they have Koilocytosize the importance of preventive measures adopted and 84

2011 5 (2)

not found cellular changes. Conclusions:The high percentage of the sample with anal sions of cervical cancer population and precursor lesions anal cancer population. It means that need for Pap screen-

the transmission usually occurred when dogs eliminate oo-

cervical cancer, allowing early diagnosis of anal cancer preA total of 59 sera samples from dogs with no neurological Keywords: Intraephitelial injuries, Cervical intraepithelial neoplasia, anal canal off point. Results: The frequency of positive samples was CPE - 1760 Etiology Survey of SubcCinical Mastitis (SCM) in Brazil Bezerra, M. J. G.¹; Silva, L. B. G.4; Mota, R. A.4 1. Discente do Programa de Pós – Graduação em Ciência Veterinária da Universidade Federal Rural de Pernambuco (UFRPE), Brasil; 2. Discente de graduação em Medicina Veterinária da UFRPE, Brasil; 3. Discente do Programa de Pós – Graduação em Biociência Animal da UFRPE, Brasil; 4. Professor Associado do Departamento de Medicina Veterinária da UFRPE, Brasil

of positive samples were reagents in the titration 1:50 and 1:60 with no associated clinical disease signs. Conclusions: The Neospora caninum infection is present in dog population examined and the male dogs showed higher positivity than females. Keywords: neosporosis, diagnosis, canines CPE – 1760b Presence of Ureaplasma sp. in semen samples of sheep from the State of Pernambuco, Brazil Silva, J. G.1; Santos. A. S.1; Kim. P. C. P.1; Santos, S. B.1; Mota, R. A.1 -

effects on production, composition, quality of milk and

genital tract, and that can affect the reproductive perfor-

Methodology. Milk samples were collected from 52 goats

Methodology: A total 50 sheep of reproductive age and

-

The DNA was extracted from the semen samples using a tions. The PCR mixture (17,25 µL) contained 5µL of template DNA; 0,5 µL primers (30pmol each); 5 µL of water and 6,25µL of MasterMix commercial kit, according to the

and Bastonete of gram-negative associated with mastitis. spp. were more common pathogens associated with in mastitis in this study. These data indicate the importance of preventive hygienic of milk utensils and teat manageKeywords: Microbiology, Milk Goat, Mammary gland CPE – 1760a Antibodies research anti-Neospora Caninum in dogs diagnosed in the Veterinary Hospital of UFRPE, Recife, Brazil Freitas, R. S. V.1; Machado, A. C.2; Silva, J. G.1; Souza Neto, O. L.1; Alves, B. H. L. S.1; Melo, R. P. B.1; Santos, A. Mota, R. A.4 1. Discente de graduação em Medicina Veterinária da UFRPE, Brazil; 2. Discente do Programa de Pós – Graduação em Ciência Veterinária da Universidade Federal Rural de Pernambuco (UFRPE), Brazil 3. Discente do Programa de Pós – Doutorado da UFRPE, Brazil; 4. Professor Associado do Departamento de Medicina Veterinária da UFRPE, Brazil Background: Neosporosis is a parasitic disease character-

animals in the region. Keywords: Ureaplasma; semen; sheep herds CPE – 1764a Healthcare Nursing to the patient with pancreatic tumor that was submitted to gastroduodenopancreatectomy Medeiros, J.H.1; Silva, D.C.A.1; Silva, D.M.1; Silva, ra, J.M.D.1; Santana, P.R.1 1. Federal University of Pernambuco, Brazil Introducion: the tumor of the pancreas has high mortaland lack of aggressiveness of the treatment really effecquestion had their diagnosis carried out after indicate lo85

Experimental Pathology and Health Sciences

calized pains in the region and complain of tumefaction, CPE – 1765a -

agulase Negative Staphylococcus Isolated Of Diverse Regions Of Health Care Professional Of Hospital Universitary

rier of the same framing a SAE as regards post-operative. Metodology: Held at the surgical clinic North Wing of the

1. Bacteriology Laboratory / Department Of Tropical Medicine University Federal De Pernambuco, Brazil Introduction: The Staphylococcus coagulase negative skin and, although they were considered saprophytes for

nesis and physical examination of the patient, information achieved with their families and health record data, mak.Resultads: The appropriate care for a patient after surgery are essential for an adequate recovery, avoiding infections or any worsening in the clinical picture. For this to need

they prevail in numerous organic situations to cause serious infections. There are currently related to numerous nosocomial infections, and the main vehicle of transmission are the hands of health care professional (HCP), who

SCN isolated from various regions (nasopharynx, hands and cell phones) of HCP. Methods: The Staphylococcus SAE post surgical assistance for the recovery of the client. Keywords: Cancer, SAE, Care

samples of HCP (doctors, nurses and nursing assistants),

CPE - 1765 agulase Negative Staphylococcus Isolated Of Diverse Regions Of Health Care Professional Of Hospital Universitary 1. Bacteriology Laboratory / Department Of Tropical Medicine University Federal De Pernambuco, Brazil Introduction: The Staphylococcus coagulase negative

using discs of penicillin (PEN), oxacillin (OXA), cefoxitin (CFO), vancomycin (VAN), gentamicin (GEN), erythromycin (ERI), clindamycin (CLI), chloramphenicol (CLO),

to CIP. Conclusions: The results of this study support the literature, showing a higher incidence of resistance pro-

skin and, although they were considered saprophytes for they prevail in numerous organic situations to cause serious infections. There are currently related to numerous nosocomial infections, and the main vehicle of transmission are the hands of health care professional (HCP), who

SCN isolated from various regions (nasopharynx, hands and cell phones) of HCP. Methods: The Staphylococcus samples of HCP (doctors, nurses and nursing assistants),

using discs of penicillin (PEN), oxacillin (OXA), cefoxitin (CFO), vancomycin (VAN), gentamicin (GEN), erythromycin (ERI), clindamycin (CLI), chloramphenicol (CLO),

to CIP. Conclusions: The results of this study support the literature, showing a higher incidence of resistance pro-

for cell phone and hands of the them in order to avoid contamination of the same or patients. agulase negative health professionals 86

for cell phone and hands of the them in order to avoid contamination of the same or patients. agulase negative health professionals CPE - 1789 Acceptability of food made from yam (Dioscorea sp.) by students of a private school in the city from João Pessoa, PB De Lima, K.N.1; De Sousa, T.A.2; Santos, J.S.2; Oliveira, S.M.S.1 2. Faculdade Santa Emília de Rodat- Biomedicina, Brazil Introduction: In the Northeast, the yam is a source of nutrients and income for small and medium farmers, as part of food, mainly rural population, possessing a variety of nutritious and tasty dishes. The development of drates, proteins, vitamins and minerals and low in fat, is a good choice of snack for children of school age. The school is where children spend a period of time every day, making it an important site for the formation of eating important it is that the school canteens sell nutritious jectives: This study aims at encouraging the consumption of yam in populations of school children, aged 7 and 12 years, through culinary innovations, such as yam pie, so as

2011 5 (2)

to achieve a healthier diet and alternative and verify the evaluation of the sensory parameters (taste, odor, texture

CPE – 1790a Eritrócitos bovinos como modelo experimental para o uso de manitol no combate a edemas: teste comparativo com outras moléculas

and a hedonic scale. Results: According to the results of pie of yam in the school meals. The results of the facial sults it was concluded that the preparations with yam had

T.C.M.3; Carvalho, M.M.S.G.2; Santos, E.P. 1. Federal University of Paraíba, Brazil; 2. State University of Paraiba, Brazil; 3. Faculdade Santa Emilia Rodat, Brazil Bovine erythrocytes as an experimental model for the use other molecules Background: Given the importance of understanding the mechanisms of maintenance of the osmolarity for stu-

of nutrition. Keywords: Acceptability; yam; students CPE - 1790 Evaluation cytotoxic against Artemia Salina and antinociceptive activity of 2-amino-4,5,6,7-tetrahydrobenzo [b] thiophene-3-carbonitrile derivative A.R.S,2; Pereira, C.K.S.2; Salgado, P.R.R.S. 2; Assis, T.S.2; Almeida, R.N. 2; Moura, R.O 1; Mendonça-Junior, F.J.B.1*. 1. Laboratório de Síntese e Vetorização de Moléculas, Universidade Estadual da Paraíba, João Pessoa 58020-540, Brazil; 2. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Caixa Postal, 5009, João Pessoa 58051-970, Brazil Background: Thiophenes derivatives are endowed with anti-fungical and other. However, it is important to know its cytotoxicity. An alternative to conventional cytotoxicity tests are the tests with the microcrustacean Artemia salina,

passively altering the intracellular concentration and, consequently, the results of the experiment. The rule extends to tissue and systemic level. According to some studies, controversy regarding the use, for example, in countering the effects of acute stroke to reduce intracranial pressure, promoting an increase in osmotic pressure of the vessels, ciency of the osmotic mannitol as compared with other molecules in order to demonstrate the effect of tone on lar solutions (glucose, mannitol, urea) and ionic (KCl and tion:

assesses the antinociceptive and cytotoxic activity of a varying the speed of water entering or leaving the cell. Conclusions. In experimental terms, the molecular nature separated ten Artemia salina newly hatched and added to and 4.5 mL of synthetic saline water. The solution was diluted in the following concentrations standard (1000 mg acid was performed using Swiss male mice, 25-35 grams, 3 months of age, divided into groups with eight animals. The control group received distilled water and tween 20, 4

sults: The thiophene derivative showed high cytotoxicity

the molecular level. Keywords: Edema; osmolarity, mannitol CPE – 1793 Histopathologic Evaluation Of Skin Wound Healing Treated With Bixa Orellana L. Extract (Annatto, 2,5% Norbixin) In Rats Santos, J.A.A.1. ; Souza, M.F.A.M.1. ; Silva, E.L.V.1. ; Aguiar Jr, F.C.A.1. 1. Laboratory of Biotechnology and Pharmaceuticals, Academic Center of Vitoria – Federal University of Pernambuco, Brazil Background: Annatto is a red-yellow dye extracted from the mature and dry seed pericarp of Bixa orellana L. (Bixa-

tractions, results very close to those found in morphine. Conclusion: The compound tested showed high cytotoxic-

-

however the results antinociceptive were considered mean Key-Words: Thiophene Derivatives, Anti-nociceptive, Citotoxity activity

effective for quenching free radicals and act as a potential antioxidant. As annatto is large used as a coloring material, -

87

Experimental Pathology and Health Sciences

were investigated in surgically-induced skin wounds of rats

thelialization and angiogenesis during skin wound healing process in rats. Keywords: wound; alpha-pinene; rats

thickness skin wounds were surgically performed on the

21st postoperative days were processed for routine hisnewly formed capillaries, and collagen deposition. For stare-epithelization on the 1st, 3rd, 7th, 14th and 21st days postoperative. Conclusions: The topical use of the annatto Keywords: Bixa orellana L.; Wound healing; Rats

CPE - 1795 Prospects of using stem cells in Alzheimer’s disease Firmino, J.M.1; Diniz, D.A.M.1; Benício, T.C.M.1; Alves, M.A.S.5; Santos, J.C.S.6; Santos, E.P.1; Souza, J.P.1,4; Carvalho, M.M.S.G.6,7 1. Santa Emilia de Rodat Faculty, student of Biomedicine, Brazil 2. Federal University of Paraiba, student of Technical Biodiagnostic, Brazil. 3. Federal University of Paraiba, student of Nursing, Brazil. 4. Federal University of Paraiba, student of Química, Brazil. 5. Unida da Paraíba Faculty, student of in nutrition, Brazil. 6. State University of Paraiba, Brazil 7. Oral Biology Laboratory, Health Science Center, Federal University of Paraiba, Brazil form of dementia among the elderly, leading to severe and genetic risk factors, acquired and environmental hypotheses are reported as causes for the development of

CEP – 1793a Biological Effects Of Topical Treatment With AlphaPinene In The Healing Of Skin Wounds In Rats Santos, J.A.A.1. ; Cavalcanti, C.C.L. 2. ; Souza, M.F.A.M.1. ; Cavalcanti, T.R.L.2.; Lima, S.M. 2. ; Araújo, R.M.4.; Ximenes, R.M.3.; Martins, R.D.2.; Aguiar Jr, F.C.A.1. 1. Laboratory of Biotechnology and Pharmaceuticals, Academic Center of Vitoria – Federal University of Pernambuco, Brazil. 2. Laboratory of Physiology and Pharmacology, Academic Center of Vitoria – Federal University of Pernambuco, Brazil. 3. Departament of Physiology and Pharmacology – Federal University of Ceará, Brazil. 4. Federal University of Rio Grande do Norte, Brazil.

standing of AD and the prospects for treatment of the same through the therapeutic use of stem cells. Methodology: It consisted of a literature review using the key

nal cortex, hippocampus and neocortex. With the discov-

species of conifers. This compound is one of the chemo-

from stem cells which, in turn, derived from primordial

urundeuva popularly known as “aroeira-do-sertão”. Several medical agents used in wound care are derived from

-

pinene has reported several pharmacological actions, such treatment with alpha-pinene in surgically-induced skin wounds in an animal model. Methodology: A full-thickness dermal excision wound was created on the shaved dorsum of third-six male wistar rats. The animals were randomly separated in three groups with 12 rats each:

of life of patients with this disease. Keywords: Alzheimer’s disease, stem cell, neurogenesis

closure was monitored using the wound-healing microand newly formed capillaries. For statistical analysis, the

CPE - 1807 Repellent effect of Indigofera suffruticosa (Fabaceae) on Aedes aegypti (Diptera: Culicidae) reproduction Lima, I.R. 1 ; Vieira, J.R.C. 1; Leite, S.P. 1 1. Departamento de Histologia e Embriologia- CCB/UFPE, Brazil Background: Indigofera suffruticosa is a plant of the Fa-

2 on the 14th day after surgery. Re-epithelialization was accelerated in groups 1 and 2 on 7th postoperative day

commonly known as an IGR) are products or materials

treated animals from group 1 and 2 on 7th postoperative -

surfaces which discourages insects (and arthropods in

88

2011 5 (2)

quency sounds). The mosquito Aedes aegypti is a vector promotes the dissemination of four serotypes of dengue purpose of this study was to investigate the deterrent activity on oviposition of A. aegypti treated aqueous extract from leaves of I. suffruticosa. Materials and Methods: During 4 consecutive days, 90 female mosquitoes of A. aegypti were stored in polypropylene cages (30 × 30 × 30 cm) (Bugdorm-I, Mega View Science Education Services,

Campos, I.A.1, Carvalho, C.R. 1, Laranjeira, L.P.M. 1, Sitônio, M.M. 1, Silva, J.B.N.F. 1, Mota, F.V.B. 1, Mesquita, A.R.C. 2, Ximenes, E.C.P.A. 2, Silva, T.G. 1 1. Laboratório de Bioensaios para Pesquisa de Fármacos – Departamento de Antibióticos/UFPE, Brazil 2. Laboratório de Bioquímica e Fisiologia de Microorganismos, Departamento de Antibióticos/UFPE, Brazil associated with a failure in the immune system host reties against pyogenics cocci and enteric pathogenic yeast.

3.5). Results: In the deterrent activity the aqueous extract the posture of eggs of A. aegypti (88 (13–210)), compared with the control in distilled water (343 (185–406)). Conclusion: This research demonstrated that the species I. suffruin the medical and pharmaceutical industries. Keywords: Aedes aegypti; Indigofera suffruticosa; repelent CPE – 1807a Behavioral evaluation in mice bearing sarcoma 180 treated with leaves of Indigofera suffruticosa Lima, I.R.1; Vieira, J.R.C. 1; Leite, S.P. 1 1. Departamento de Histologia e Embriologia- CCB/UFPE, Brasil Background: Indigofera suffruticosa is a plant of the aqueous extract and Indican isolated from I. suffruticosa showed that plant has antitumor activity. Behavioral responses in mice are important tools to detect toxic effects

the development of sepsis on animal models. Methodology: A total of 36 mice Mus musculus were divided into 3 groups. The animals received administrations (p.o) twice a gation and puncture (CLP) was performed on the 11º day. The sham group underwent the surgical procedure without induction of sepsis. Six animals of each group were neal cavity washed with PBS+EDTA (3 mM). Half of the animals from each group were left to assess the survival rate. The leucogram was realized with hematologic anascience). The statistical analysis was made using ANOVA

increase of survival rate and increased the leukocytes migration (6.32±0.47) comparing with groups (4.40±0.60) ZM (66.45±2.60) comparing with control (57.26±2.68) and sham (20.86 ±0.47). IL-10 levels increased in ZM (2574.04±81.12) comparing with control (1328.48±6.29) and sham (52.95±1.54). Conclusion: In agreement with

with the aqueous extract and Indican from leaves of I. sufmusculus), male, young adults, 60 days old, weighing from

intraperitonially). It was injected into the right axillary 0.3 mL (around 3x106 cells) of the ascitic form of Sarcoma

minutes to 4 hours after administration of the extract and cervical dislocation. Results: After the end of the toxicoaqueous extract and Indican from leaves of I. suffruticosa showed some stimulant and depressor actions in mice sion: It follows that the aqueous extract and the Indican from leaves of I. suffruticosa showed some alteration of Nervous System. Keywords: Indigofera suffruticosa; sarcoma 180; animal behavior. CPE – 1808 Zymomonas mobilis improves survival rate in CLPinduced lethal sepsis in mice

against pathogenic microorganisms. Keywords: Zymomonas mobilis; probiotics; sepsis CPE - 1810 Histological changes of the adrenal gland of pinealectomized female rats Sousa, I.D.D.¹; Dias, S.M.S.¹; Santos, K. R. P.¹ 1. Laboratório de Biotecnologia e Fármaco - UFPE/CAV, Brazil there are still divergences in these results, since there is no consensus on which cortical layer suffers such changes. tological changes the adrenal gland of 45-day-old pinealectomized rats. Methodology: We used 20 female rats of Wistar lineage. The animals were divided in two groups: pinealectomized rats and sham-pinealectomized rats (control). At the end of the experiment (7 months after surgery), the animals were anesthetized and euthanized, the mitted to the hematoxylin-eosin staining and examined under light microscopy. Results: The pinealectomized female rats showed increased vascularization of all cortical layers. Was detected a larger development of the zona fas89

Experimental Pathology and Health Sciences

to the same cortical zone in the sham-pinealectomized

Co. Ltda. Despite its increasing use, its side effects should

the pinealectomy, provokes changes in the histological structure of the adrenal gland in rats, specially in the zona

reports of sexual dysfunction in erection, ejaculation and effects of treatment with olanzapine in mice Mus muscu-

physiopathology. Key-words: Pinealectomy; Adrenal glands; histology. CPE – 1811 Action of the fractions of species of the genus Vernonia in the inhibition of gelatinase A Moreira, G.A¹; Matos, L.G.¹; Longatti, T.R. ; Oliveira, 1. Laboratório de Biologia Celular e Mutagênese - LabCcel/CCO/ UFSJ, Brazil 2. Molecular Oncology Research - Barretos Cancer Hospital, Brazil. ases (MMPs). These enzymes are known to degrading extracellular matrix components, and MMP-9 (gelatinase A) Vernonia in the action of the gelatinase A. Methodology:

Vernonia: hydroalcohol, hexane, chloroform and ethyl acsitometry was performed using Scion Image. Phytochemi-

morphometric analysis of this organ. Methodology: We ceived deionized water while the treatment groups were col methacrylate (Leica). Histological sections of 4 mm analyzed morphologically and morphometrically. Results: ences regarding the height of the villus epithelium of the seminal vesicles, when compared the control group and 20 mg treated group, where you realize that there was a reduction in seminal vesicle epithelium in the group treated with 20 mg olanzapine in the control group. Conclusion: the relevant parameter analyzed the seminal vesicle, this relevant information for patients who are dependent on their use. Keywords: Olanzapine; Seminal vesicle; mice CPE - 1814 Determination of the presence of polysaccharide capsule and resistance genes in pathogenic isolates of Klebsiella pneumoniae

in the extracts were performed according to Matos (1988).

alkaloids. The proteolitic activity of gelatinases was visual-

hexane fraction increased the activity of gelatinase A in droalcoholic fractions of extracts of specie of Vernonia -

1. Universidade Federal de Pernambuco - UFPE, Brazil 2. Departamento de Parasitologia; Centro de Pesquisas Aggeu Magalhães - CPqAM / FIOCRUZ, Brazil 3. Laboratório de Imunopatologia Keizo Asami - LIKA / UFPE, Brazil 4. Departamento de Medicina Tropical - Universidade Federal de Pernambuco- UFPE, Brazil cause urinary tract infections, septicemia, pneumonia and meningitis, especially in hospitals environments. The most

action of gelatinases. Keywords: Gelatinases, Zymography, plant extract CPE - 1813 Effects of Olanzapine on the use of the seminal vesicles of mice Mus musculus Silva, G. G. A.1; Amorim, M. J. A. A. L.2; Amorim Junior, A. A. 3; Silva Junior, V. A.;4 Santos, C. R. O.5 1. Federal Rural University of Pernambuco - Brazil 2. Federal Rural University of Pernambuco - Brazil 3. Federal University of Pernambuco - Brazil 4. Federal Rural University of Pernambuco - Brazil 5. Federal Rural University of Pernambuco - Brazil Background: Olanzapine is an atypical antipsychotic used to treat acute and maintenance therapy in the frames of schizophrenia and other psychoses, is currently marketed 90

of drugs for clinical treatment. Additionally, pathogenic isolates of K. pneumoniae usually produce a large amount of capsular polysaccharide (CPS), which confers resistance resistant isolates of K. pneumoniae with ESBL phenotype. Methodology: For this study, it was used four nosocomial isolates of K. pneumoniae, the K15C, K15R, K16R e

-

2011 5 (2)

-

have the gene for production of CPS, showing high viruisolates at hospital environment. Conclusions: The isolates tested are highly virulent due to the presence of CPS.

main agent of the disease is Fonsecaea pedrosoi, followed mated onset of the disease in approximately 163 000, con-

the infection treatment. ride capsule CPE - 1815 Association between multiple infections and vaccines for human papillomavirus in women with cervical lesions

ation in the Brazilian population. Methodology: We con-

A.1, Silva-Neto, Jc.1. 1 - Laboratory of Clinical Cytology of the Federal University of Pernambuco - Brazil. Introduction: It is known that HPV is associated with the prevalence of viruses HPV-6, -11, -16 and -18. However, other important oncogenic viruses, as well as associations of viruses in the same host can effectively participate in the development of high-grade lesions and invasive. pare with existing vaccines. -

-

Keywords: Chromoblastomycosis; subcutaneous mycosis CPE – 1816a Congenital malformations in the municipality Caruaru-PE 1. Faculdade ASCES, Brazil 2. Universidade Federal Rural de Pernambuco, Brazil 3. Laboratório de Genética e Genômica, USA Background: The Congenital Malformations are morpho-

the use of PCR-RFLP were detected HPV-DNA and the samples were genotyped for viral types 6, 11, 16, 18, 31, 33

-

I, Hae III, Rsa I, Pst I) in a sample collected with cervical -

-

-

maternity Jesus Nazareno in the municipality Caruaru-PE. Methodology: A sectional study was performed on new-

-

according to affected systems according to codes Q00.0

outside the range.

congenital malformations were compiled in spreadsheets, for the year and the period studied. Results: In the analysis -

ies to analyze the spectrum of current vaccines immunization aiming at the detection of cross-reactions. Keywords: vaccine, genotyping, multiple infections.

Analysis of congenital malformations showed a predominance of systems of musculoskeletal apparatus, followed

CPE - 1816 Chromoblastomycosis: a reality brazilian population Silva Xavier, G.M.¹; Silva Melo, W.E.¹; Ferreira Júnior, D.L.¹ 1. Faculdade ASCES, Brazil

-

congenital deformities of the foot) presented the highest tions of the musculoskeletal apparatus and genital system. The prevalence of malformations in the municipality of Caruaru and surrounding regions was lower, When com-

and verrucous plaques that may ulcerate. The fungus pen-

of information. Keywords: Congenital malformations; Newborns; Caruaru

ease is the most important part of the infectious diseases

CPE – 1816b Implementation of a laboratory protocol for diagno91

Experimental Pathology and Health Sciences

sis of mucopolysaccharidosis Silva Melo, W.¹; Xavier Silva, G.¹; Ferreira, C.S.¹; Esteves 1. Faculdade ASCES, Brazil 2. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, Brazil Background: Mucopolysaccharidosis (MPS) are rare gecosaminoglycans (GAG). When they are not degraded, variety of pathological complications, starting from the fetal period until the infant stage. The diagnosis of MPS ation. There are no epidemiological data on this disease in Brazil, which makes recognition of the disease among measuring enzyme in the investigation of MPS allows the

documented in the literature, misdiagnosis is common due

treatment. Keywords: Paracoccidioidomycosis; Tuberculosis; Diagnosis. CPE - 1833 Evaluation of the in vitro leishmanicidal activity of the ethanolic extract of Artemisia vulgaris Barreto,M.M.L.1; Soares,P.H.S.2; Catena,A.S.1; Soares,L.L.1; Almeida,E.R.2 ; Medeiros.P.L.1; Silva,E.C.1 1.Laboratório de Cultura de Tecidos/Depto. de Histologia e Embriologia - CCB/UFPE, Brazil 2.Depto. de Antibiótico - CCB/ UFPE, Brazil treatment of leishmaniasis arises due the toxicity and the emergence of resistant parasites to drugs already market-

of MPS. Methodology: The study was conducted through To investigate the antileishmanial activity of the ethanolic extract of Artemisia vulgaris. Methodology: Artemisia thin layer chromatography and research urinary GAG) and

fests and thus provide guidance in developing appropriate treatment protocols. The implementation of a proto-

of dry chopped leaves in 1L of ethanol. After 72 hours, conducted in thin layer chromatography. Promastigotes (Leishmania amazonensis and Leishmania major) were

Keywords: Mucopolysaccharidoses; Differential Diagnosis

(initial concentration), and the test was done in triplicate. The ethanol was used as negative control. The parasites

CPE – 1816c Paracoccidioidomycosis and tuberculosis: differential diagnosis Silva Melo, W.¹; Silva Xavier, G.M.¹; Oliveira, R.F.¹; Alves, P.A.¹; Santana, K.S.¹; Almeida Sanatana, W.¹ 1. Faculdade ASCES, Brazil losis (TB) may occur simultaneously or sequentially. The clinical presentation of disease can coexist, due to the overlapping of the epidemiological and clinical stories. Er-

48 and 72 h. Results: Essential oil (linalool, cineol, thu-

form of two diseases and forged a consensus in the dif-

of sesquiterpene (vulgarina), tannin, resin and inulin were nensis. Conclusion: the ethanolic extract of leaves of Artemisia vulgaris has leishmanicidal activity, which suggests Keywords: leishmanicidal activity, medicinal herbs, Artemisia vulgaris CPE – 1834 Evaluation of wound healing potential of royal jelly Costa, E.M.¹ Guimarães, B.J.E.1; Teixeira, R.R. 2; Pru-

Results: According to the data and articles analysed, TB and PMC can compromise a single individual, and this asthe presence of the fungus P. Brasiliensis (PMC) or of the -

92

R.I.M.A. 1 1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ, Brazil 2. Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Brazil. Background: Wound healing corresponds to the tissue rejectives: To evaluate the effects of topical and oral use of royal jelly in cutaneous wound healing. Methodology: 20 previously anesthetized mice Swiss were used. A circular

2011 5 (2)

mice with the use of scissors and forceps, and the wounds were treated and measured daily for 20 days. Groups of 4 mice were given the following treatments: control (C- non

effect on Mg and K serum levels during acute phase leptospirosis in hamsters. Keywords: Leptospirosis, Leptospira, Animal disease models CPE – 1837

control (C2). The wound tissue was processed for histological examination after 24 hours, 4 days, 7 days, 14 days, and 20 days. Later, the slides were stained with hematoxylin and eosin (HE) and taken to optical microscope analysis. Results: In the RJC and RJO groups, on the 20th day, for the other groups. As for edema formation, the RJO group showed less formation. However, on the 14th day, all animals showed very similar edematous aspect. From which still showed it was the RJO. Dermal appendages were seen earlier on animal of the VC group, with all of them showing appendages on the 7th day. With relation to re-epithelialization, the process occurred faster in groups day. Conclusion: The use of topical or oral royal jelly does not show any relevant interference in the healing process. Keywords: wound healing, royal jelly, wounds. CPE - 1836 Experimental leptospirosis in hamsters induce hypomagnesemia and hyperkalemia in acute-phase disease Santos, C. S. 1,2; Azevedo, E. C. 2; Soares, L. M. 2; Seixas,M. 1; Bandeira, M. 2; Amorim, D. M. 2; Chagas Jr, A.D. 1;Alexander McBride, A. J. 1; Cruz McBride, F. W. 2; Reis, M. G. 1,2; Athanazio, D. A. 1,2 1. Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador, Brazil. 2. Federal University of Bahia, Salvador, Brazil. Introduction: Patients with severe leptospirosis develop hypomagnesemia during acute phase disease as well as sodium and potassium wasting defects. The non-oliguric renal failure rapidly evolves to an oliguric hyperkalemic form that denotes poor outcome. The renal waste of Mg, Na,

HPV by electrochemical biosensors Zanforlin,D.M.L1,2, Campos-Ferreira,D.S1,2, Nascimento, G.A1,2, Arruda, M.S 1,2. Souza,E.V.M1,2, Oliveira,N.C.L 1,2.Castelletti,C.H.M1,2, Bruneska,D.1,2,3, Lima-Filho,J.L1,2,3 1. Federal University of Pernambuco, 2. Laboratory of Immunopathology Keizo Asami,LIKA, 3 Department of biochemistry of Federal University of Pernambuco Studies show that infections with human papillomavirus (HPV) of high-risk can lead to the development of tumors of the HPV is extremely important for a successful treat-

series of alignments among the HPV genomes to locate consensus sequences of this virus. Finally, it was made a -

found 26 consensus sequences. Among these, 7 sequences The study comparative with the PapilloCheck showed that tect 18 types of high-risk HPV. We could conclude that

detect HPV will have a simpler methodology than availKeywords: Biosensor, DNA, HPV

study the dynamics of Mg, Na and K serum levels during acute experimental leptospirosis. Methods: Hamsters were vals, for kidney lesions and evaluation of serum levels of NO, K, Mg and Na. The intervals were 4, 8, 16 and 28 days after infection. Hamsters were separated in four groups according to the treatment started on the tenth day: ampi-

from day 4 to day 16. In addition, all groups experienced a levels of Na remained unaffected among different interof serum levels of Na in the MB group at day 16. Conclusions: Hamsters reproduce the hypomagnesemia during

CPE – 1837a Development of DNA probes to detect the serotype DENV-4 using electrochemical biosensors Oliveira,N.C.L 1,2,Zanforlin,D.M.L1,2, CamposFerreira,D.S1,2 , Nascimento,G.A1,2 , Arruda, M.S 1,2. Souza,E.V.M1,2, Lima-Filho,J.L1,2,3 1. Federal University of Pernambuco, 2. Laboratory of Immunopathology Keizo Asami,LIKA, 3 Department of biochemistry of Federal University of Pernambuco the world with approximately 100 million people infectfamily Flaviviridae, with 4 distinct serotypes (DENV-1 to DENV-4). Currently in Brazil the most prevalent seThe reemergence of this serotype in Brazil along with the simultaneous circulation of other genotypes of dengue increases the appearance of severe manifestations of this disease, like dengue hemorrhagic fever (DHF).The early ease and to take preventive measures. Herein, we report

production. Conversely, hypokalemia was not reproduced in this model in which all groups developed increasing lev93

Experimental Pathology and Health Sciences

tional Center of Biotechnology Information (NCBI). The

-

of analyses of genome. The criteria for selection of the se-

portuguese), and the patients operated for esophageal

mismatches and hairpins.¬¬ As results, a total of 80 DNA sequences were analysed. Among these, 15 were chosen as -

Encephalopathy .Methodology: data collection took place through research of collecting critical information developments and medical prescriptions and nurses, and clini-

detection of dengue. Key-words: Dengue , DNA, electrochemical biosensors CPE – 1838 Prevalence of Human Papillomavirus 35 in highgrade intraepithelial lesions and invasive female genital tract F.1, Silva-Neto, Jc.1. 1 – Laboratório de Citologia Clínica da Universidade Federal de Pernambuco – Brasil.

Cesário (normal), primary mother , who had hypertension crisis; the child had delay in development of crying specialized hospital and staying therer during 34 days in agnosed with GERD associated á ECNP, evolving with Bronchopneumonia and Chronic Dyspnea, Pneumonia reranitidine, famotidine and nizatidine), evolving as well. Conclusion: Although the infant have evolved positively to prospective and longitudinal casuistic designs, with the operated with DRGE and ECNP.

of the etiology of cervical cancer and the types 16 and 18

ated; not progressive chronic encephalopathy

HPV 6, 11, 16 and 18. Some studies report cross-reactions with type 45, and others types 31 and 33. HPV-35 is not included in the vaccination plan and do not suffer from immunological cross-reactions. in women with high-grade cervical lesions and invasive. Methodology: We analyzed 89 samples of cervical lesions on the type of HSIL and 37 invasive lesions (squamous cell and adenocarcinoma). Through the use of PCR-RFLP

Results: Of 126 cases analyzed, HPV-35 was present in

CPE – 1840a Aroeira do Sertão (Myracrodruon urundeuva fr. All): assessment of the effects of Toxicology and healing chemotype of essential oil Alpha-pinene Silva, D. M. ¹; Silva, D. C. A. ¹; Cavalcanti, T. R. L ¹;Lima, S. T. H. L.¹; Medeiros, J. H.¹; Sousa, M. A.¹ 1. Federal University of Pernambuco, Brazil 2. Federal University of Rio Grande do Norte, Brazil Introduction: Aroeira(mastic) is a common name for sevwhich the Myracrodruon urundeuva fr. Allemão is popularly known in Brazil as aroeira-do-sertão. These species have many therapeutic uses, with actions such as ovarian,

Conclusion: The present study demonstrates that a sigHPV can is associated with type 35 which is not part of the spectrum vaccine immunization now. The analysis of spectrum are needed for the implementation of vaccination campaigns. Keywords: PCR-RFLP, HPV genotyping, cervical lesion

oils D-Limonene (LIM) and Alpha-pinene (PIN) extracted from the leaves of Myracrodruon urundeuva. Methodolsurgery. After the demarcation of the area, the skin was intotal resection. The animals were treated with PIN in the (CRP), used in dilution of essential oils. Prior to the sacri-

CPE - 1840 ciated With Not Progressive Chronic Encephalopathy Silva, D. M. ¹; Silva, D. C. A. ¹; Lira, M. C. C. ¹; Silva, L. G.

and total protein. Results: among the groups control with Cremophor and distilled water there was statistical differ-

J. H.¹; Sousa, M. A.¹ 1. Federal Universtiy of Pernambuco, Brazil sociated with symptoms of diseases and pulmonary lower airway such as aspiration pneumonia, asthma, chronic 94

sion: the use of essential oil for PIN in situ treatment of

2011 5 (2)

wounds, lifting discrete parameters reveals toxicity tested, the treatment. Keywords: aroeira-do-sertão; toxicity; healing. CPE – 1841a

Promastigotes (Leishmania amazonensis and Leishmania

Cases of Pulmonary Tuberculosis in Children: An Integrative Review Silva, D.C.A.1; Silva, D.M.1; Sousa, M.A.1; Silva, L.G.A.1; S.Q.1 1. Universidade Federal de Pernambuco / Victoria Academic Center- UFPE / CAV, Brazil

the plates at concentrations of 62, 125, 250, 500 and 1000 triplicate, and DMSO was used as negative control. After

of life, especially in patients with HIV. It constitutes, nevcomplicates the the clinical suspicious and delayed diag-

manicidal activity against the species L. major and L. ama-

pulmonary TB in children are asymptomatic, while others have cough for more than two weeks, fever, malaise and Keywords: Leishmania major, Leishmania amazonensis, copaiba oil integrative literature review as a method to this investigaish. Results: We selected 11 of 32 items found searching Thus, according to scoring system (MSB) of health Min-

and limited effectiveness of diagnostic tests of childhood TB, several scoring systems (score) were developed. However, these instruments have a wide variation in sensitivity, oped a scoring system for the diagnosis of pulmonary tu-

CPE – 1843a Segmentation of infections origin and topography in patients admitted to the Emergency Clinic of a large public hospital - Recife - PE 1. Microbiological Analysis Laboratory, Department. Pharmaceutical Sciences - Federal University of Pernambuco, Brazil 2. Hospital of the Restauração, Agamenon Magalhães Av S / N, Brazil Background: The emergence of new resistance and spread indications aggravating infectious from hospitals or comstudy evaluated the incidence of infections, segmenting

reporting of cases. Keywords: Pulmonary tuberculosis; childhood; diagnosis. CPE – 1842 Leishmanicidal activity of commercial oil Copaiba Barreto,M.M.L.1 ; Anjos, J.A.L2 ; Cassilhas, A.P.P3 ; Amorim, R.V.S1 ; Silva, L.L.S1; Medeiros, P.L1 ; Silva, E.C1 1. Laboratório de Cultura de Tecidos/Depto. de Histologia e Embriologia - CCB/UFPE, Brazil 2. Núcleo de formação docente (NFD)/Centro Acadêmico do Agreste (CAA)/UFPE/Brasil 3. University Salgado de Oliveira – UNIVERSO, Brazil Background: Cutaneous leishmaniasis is an anthropoLeishmania. Parasitic infections are part of our regional reality and therefore deserve special attention in the revarious species of the genus Copaifera (Leguminosae) and

Emergency Clinical Hospital of Restauração, which used tion of infection was made according to the topography, they were: respiratory, gastrointestinal, urinary, central nerthe community and the hospital. Results: With regard to

the work was done and need for future epidemiological cording to the prevalence in origin, there was a predominance of infections of Community origin. Conclusions: The results of the study justify the importance of continthe CCIH. Keywords: Nosocomial Infections, Antimicrobial; 95

Experimental Pathology and Health Sciences

Hospital of Restauração

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CPE – 1843b Determination of tannins and total polyphenols and toxicological study of the leaves of Schinopsis brasiliensis Engl. in the Artemia salina Leach Batista, D.C.A.1; Castro, R.H.A.1; Coutinho, F.N.1; Pessôa, ho, T.J.S.3; Amorim, E.L.C.3; Xavier, H.S.2; Pisciottano, M.N.C.1* 1. Microbiological Analysis Laboratory, Department. Pharmaceutical Sciences, UFPE, Brazil 2. Laboratory of Pharmacognosy, Department. Pharmaceutical Sciences, UFPE, Brazil 3. Natural Products Laboratory, Department. Pharmaceutical Sciences, UFPE, Brazil -

doses utilized in the acute toxicity. Calculation of the using computerized linear regression analysis, in GraphPad Prism, version 4.0. Results: All doses studied, analgesia was the more predominant effect that was more pronounced in animals treated intraperitoneally. The nerolidol presented toxic effects when administered i.p. The LD¬50

CNS with high toxicity when administered intraperitoneally and without apparent toxicity when administered orally Keywords: Nerolidol, toxicity, psychopharmacology screening CPE – 1851a

-

rivative Im-7

polyphenols and tannins and evaluate the toxicity against Artemia salina Leach methanol extract from leaves of S. levels of total phenols and residual methanol extract from

za S. A. 2; Athayde-Filho, P. F. 2; Almeida, R. N. 1; Morais, L. C. S. L. 1 1 .Laboratory of Pharmaceutical Technology, Health Sciences Center, Federal University of Paraíba 2. Chemistry Department, Federal University of Paraíba ment of partial and generalized tonic-clonic seizures.

cating that the extract showed moderate levels of toxicity, resulting in decreased movement of the larvae of Artemia

of tannins. Keywords: Schinopsis brasiliensis; Artemia salina; tannins CPE - 1851 Pharmacological behavioral screening and acute toxicity of Nerolidol in mice A. R. S.1; Salvadori, M. G. S. S.1; De Sousa, D. P.2; Morais, L.C.S.L.1; Almeida, R.N.1 1- Laboratory of Pharmaceutical Technology, Federal University of Paraíba , Brazil 2- Department of Physiology, Federal University of Sergipe, Brazil Background: Nerolidol is a sesquiterpene present in the

toilet soaps and other toiletries as well as in non-cosmetic jectives: The aim of this study was to carry out a phar-

anticonvulsant effect of the hydantoin derivative IM-7 (5-(4-isopropilfenil)-3-phenyl-imidazolidine-2,4-dione) in pentylenetetrazole (PTZ) and maximum electroshock (MES) induced seizures in mice. Methodology: Swiss al-

dard drugs for MES and PTZ tests, respectively). Thirty (phenytoin and diazepam) or sixty minutes (vehicle and IM-7) after treatment, animals were tested. In the PTZ seizure were recorded. For MES test, mice received an ear clip electrodes: seizure duration and mortality were the parameters registered. Results: Doses of 150 and 300

121.8 versus 93.3 ± 11.5 sec in control). In MES test, the zure (6.6 ± 1.5 versus 16.3 ± 0.7 sec in control) however,

kg. Conclusions: According to these results, high doses of hydantoin derivative IM-7 showed an anticonvulsant effect that was associated with increased mortality. Further

weighing 25-35 g, approximately 3 months of age were source of mortality. Keywords: IM-7; Anticonvulsant effect; Toxicity. administration (i.p.) in doses of 75, 100, 150, 175 and 200 CPE – 1851b 96

2011 5 (2)

Im-1, Im-3 And Im-7 za S. A. 2; Athayde-Filho, P. F. 2; Almeida, R. N. 1; Morais, L. C. S. L. 1 1. Laboratory of Pharmaceutical Technology, Health Sciences Center, Federal University of Paraíba 2. Chemistry Department, Federal University of Paraíba the presence of the imidazolidine ring in their structure, have shown several pharmacological activities such as anticonvulsant, miorrelaxant, anti-tumor and anti-arrhythcontaining strips of paper was done after 24 hours at room (5-(4-methylphenyl)-3-phenyl-imidazolidine-2,4-dione), IM-3 (5-(4-etilfenil)-3-phenyl-imidazolidine-2,4-dione) and IM-7 (5-(4-isopropilfenil)-3-phenyl-imidazolidine-2,4-

the isolates showed growth during the 06 months inventory, while the 12-month period, the rate of growth fell to Keywords: Conservation, adsorption, bacteria

vous system. Negative results permit to reject the tested hicle (distilled water and Tween 80) or different doses (50, changes were evaluated comparing treated groups with control group at 30, 60, 120, 180 and 240 minutes after administration. Results: All doses of IM-1 reduced amduration of analgesic effect was increased according with the doses (60, 120 and 180 minutes, respectively), and this

CEP – 1862a Toxicological Reproductive Study of usnic acid from Cladonia substellata in Famale Wistar Rats Silva CR1, Silva MM1, Rodrigues MCS1, Santos KRP1, Santos NPS1, 2, Aires CAM3, Silva RL4 1. Laboratory of Biotechnology and Pharmaceuticals / Universidade Federal de Pernambuco - Centro Acadêmico de Vitória, Brazil 2. Laboratório de Imunopatologia Keizo-Asami (LIKA), Universidade Federal de Pernambuco, Brazil 3. Institute of Biological Sciences, University of Pernambuco, Brazil 4. Faculdade Maurício de Nassau, Brazil -

-

-

tion, lower touch response and ataxia from 120 to 240 min-

were investigated after the treatment of pregnant female rats with the usnic acid. Methods: Twelve animals were

drugs. Further experiments are necessary to characterize this effect and try to draw a structure-activity relationship. Keywords: Hydantoin; Behavior; Depressant effect

via oral gavage, on days 6 at 10 of the gestation. Animals from the control group received distilled water through the same via. Th signs of toxicity. Maternal characteristics were assessed as -

CEP – 1862 Aires CAM1, Silva JD.2, Silva RL3, Silva CR.4 1. Institute of Biological Sciences, University of Pernambuco, Brazil 2. Brazilian Society of Clinical Analyses - PE, Brazil 3. Faculdade Maurício de Nassau, Brazil 4. Laboratory of Biotechnology and Pharmaceuticals - Centro Acadêmico de Vitória - Universidade Federal de Pernambuco, Brazil -

day 20 of gestation and fetuses were removed. The fetal -

er treated females showed a re

characterization, conservation and use of microorganisms are virtually essential for the development of processes for

22 isolates from different species or genus were grown in 24 hours prior to growth and 24 hours at room tempera-

control group. Conclusions: In conclusion, the evidence points to an oral reproductive toxicity of usnic acid. News cal studies. Keywords: Lichen, usnic acid, toxicological reproductive CEP – 1866 97

Experimental Pathology and Health Sciences

Effects of plant extracts in the skin wound healing process Franco, C. S. 1; Thomé, R.G. 2; Prudente, H. M. 1; Santos, 1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ, Brazil 2. Laboratório de Processamento de Tecidos – LAPROTEC, CCO/UFSJ, Brazil Background: Currently, a great diversity of plant extracts

were performed using scanning and transmission electron tion indicator) releases of treated-cells was measured during two hours. All the extracts tested were active against

resulted in synergistic effects in eight ratios tested, while extracts under experimental conditions is rarely demonextracts in the healing process. Methodology: An incision was made in male mice (Swiss) 4-6 weeks of age and

-

niques, and were stained with hematoxylin and eosin. For analysis of wound healing, were considered the presence

extract on the cell surface. The protein release pattern was L. ferrea and P. moniliformis anti-S. aureus actions. These

quantify the edema, was used a score: zero (physiological), one (tenuous), two (moderate) and three (severe). The

Keywords: S. aureus; Anadenanthera colubrina , Libidibia ferrea and Pityrocarpa moniliformis

zero (physiological), one (tenuous), two (moderate) three (severe) to four (very severe). Results: Regarding neoepi-

CEP – 1869 lagen in cutaneous scars in mice

for G1 animals presented closed wound while G4 showed no re-epithelialization. Considering the skin appendages in showed cutaneous structures while in G4 this appendages -

1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ, Brazil Background: The ulcer healing is a multifactorial phenomlular and molecular events in complex ways. The use of recent years with the search for active ingredients that play effective role in this phenomenon. For this reason, Brazil,

results compared to other treatments in the skin wound healing process. Keywords: Wound healing skin, Plantago sp and Siparuna sp extracts, medicinal plants CPE – 1868 Caatinga plants as weapons against Staphylococcus aureus: insights into action mechanisms and evaluation of synergetic effects with erythromycin Cunha, CRA 1, Paiva, M 3, Araújo, JM 1, Figueiredo, RCB 2, Silva, MV 1, Correia, MTS 1. 1 Universidade Federal de Pernambuco, Brazil 2 Centro de Pesquisas Aggeu Magalhães, Brazil. 3 Centro de Tecnologias Estratégicas do Nordeste, Brazil terium, developing increasing patterns of resistance which ered the most important human pathogen in the twentyaureus action of hydroalcoholic extracts from fruits of

induced in mice and treated with plant extracts. Methodolof Swiss line in which were caused cutaneous lesions. The daily treatment were applied topically and consisted of lyophilized extracts of two species of the Plantago (G1) and Siparuna (G3) in aqueous solution, one group received the treatment, and another group received only water. The daily application of the treatments were performed on top the dorsal region of each animal. After 9 days of therapy the animals were anesthetized and killed. Tissues were collected for histological analysis, with the purpose of quantiof the slides were acquired and the amount of collagen was analyzed with the use of a microscope (Axio Scope. A1 – Zeiss) coupled to a camera (AxioCam MRc5 - Zeiss) and a software (Axiovision 4.8.1). The amount of collagen was measured in comparison with the total area, expressed in percentage.

Conclusions: Plantago sp and Siparuna sp did not intertion. The synergetic effects of extracts and erythromycin

98

process. Keywords: wound healing, collagen CEP - 1871

2011 5 (2)

Evaluation of collagen production during cutaneous wounds healing treated with royal jelly: experimental study Guimarães, B.J.E. 2; Teixeira, R.R. 2; Prudente, H.M. 1, Carvalho, M.M. 1; Ferreira, M. 1; Oliveira, R. J.3; Espin1. Midwest Campus, Federal University of São João Del Rei, Brazil. 2. Instituto de Genética e Bioquímica, Federal University of Uberlândia, Brazil 3. Molecular Oncology Research - Barretos Cancer Hospital, Brazil. Background: Wound healing is a dynamic process which relates a series of cellular and molecular events which aim

with signalizing pathway in cell growth and studies currently suggested that Na,K-ATPase mediated cell signaling squamous cell carcinoma of oral cavity through immunohistochemistry assay and their relationship with tumour positive. The immunostained was depending of histo-

work is to evaluate the production of collagen in cutaneous wounds in mice treated with topical and oral royal jelly (RJ). Methodology: Mice were anesthetized to allow removal of cutaneous tissue. Groups with 4 animals were -

differentiated and poorly differentiated, respectively. Conclusion: Well differentiated tumour have high expression -

no treatment to wound (C); and oral suspension treat-

with prognosis in oral cavity cancer.

RJ (C2). Treatment was carried out daily. The wound tissue was processed for histological examination after 24 hours, 4 days, 7 days, 14 days, and 20 days. The slides were stained es of the slides were acquired and the amount of collagen was analyzed with the use of a microscope coupled to a camera and a computer with the software Axiovision 4.8.1. taken on each slide and the amount of collagen was ex-

CPE - 1877 Antigelatinolitic Activity of Plant Extracts Used in Traditional Medicine 1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ, Brazil. 2. Molecular Oncology Research – Barretos Cancer Hospital , Brazil Background: For the metastasis of cancer cells to occur, MMPs, especially gelatinases (MMP-2 and 9), are impor-

compared to the control group (C2). The amount of collagen produced within this period corresponds to the colapproximately. Conclusions: In general, the treatment with ertheless, results suggest that oral treatment with RJ poten-

crude extracts of plants on the activity of gelatinases and -

inducing of wound. Keywords: wound healing, collagen, royal jelly

-

CPE - 1876 subunit in squamous cell carcinoma of oral cavity cancer Silva, A. G.1; Costa, M.Z.O.4, Chaves, A.L.F. 4; Muniz, L. 1. Laboratório de Biologia Celular e Mutagênese, CCO/UFSJ, Brazil 2. Laboratório de Processamento de Tecidos – LAPROTEC, CCO/UFSJ, Brazil 3. Laboratório de Bioquímica Celular, CCO/UFSJ, Brazil 4. Grupo de Estudos e Pesquisas em Cancer de Cabeça e Pescoço UFSJ. Brazil Background: Squamous cell carcinoma is the most comdifferentiated, moderately differentiated or poorly differentiated. However, studies regarding prognosis for this -

Scion Image. Phytochemical tests to detect the presence teroids, triterpens, coumarins, saponins and alkaloids were performed according to Matos (1988). Results: The MMPtract. The extract of the species of the genus showed that found in greater quantity in Astronium and Tapirira. The

research group is currently concentrating on carrying out

99

Experimental Pathology and Health Sciences

Keywords: Cerrado, Plants extracts, gelatinases CPE - 1878 -

used to extract DNA and amplify the L1 conserved region -

rlich tumor and popliteal lymph nodes in mice Ferreira, A.S.1; Souto, C.M. 1; Pinto, F.C.H. 1; Alves, S.L.G. HPV16 sequence was used as positive control and ul1. Laboratório de Biologia Celular e Mutagênese – UFSJ, Brazil 2. Laboratório de Patologia Comparada - LPC, - ICB/UFMG, Brazil 3. Laboratório de Síntese Orgânica – CCO/UFSJ, Brazil Background: Several synthetic molecules are used in anitures: a steroidal nucleus, an unsaturated lactone (jointly growth of Ehrlich Tumor and popliteal lymph nodes in mice after administration of synthetic cardiac glycosides (DGB6). Methodology: Swiss mice were divided into three imals received daily treatments. The animals in groups G1 respectively and the control group (C) received 0.01 ml of saline throughout the experiment. Tumor growth was asof a caliper every two days until the end of the experipopliteal lymph nodes were removed for histopathological the weights paw tumor, with the contralateral paw. Results: groups. In the microscopic evaluation of tumors, G2 showed greater area of necrosis than other groups. There tween the treated and untreated groups, visualized delicate stroma and large cellular and nuclear pleomorphism. The histopathological analysis indicated the presence of metastases in the popliteal lymph nodes in all groups. ConcluDGB6 did not alter tumor growth, the morphology of the tumor mass and metastatic potential. Keywords: Ehrlich tumor, cardiac glycosides CPE – 1879 Retrospective evaluation of the incidence of HPV inby nested PCR Silva, A.M.S.1; Limeira, C.2; Lima Filho, J.L.1,3; Magalhães, V.4; Bruneska, D.1,3; Araújo, R.F.F.1,3 1. Laboratório de Imunopatologia Keizo Asami-LIKA, Universidade Federal de Pernambuco, Brasil; 2. Hospital Oswaldo Cruz – Universidade de Pernambuco, Brasil; 3. Departamento de Bioquímica – Universidade Federal de Pernambuco, Brasil; 4. Departamento de Medicina Tropical – Universidade Federal de Pernambuco, Brasil Introduction: Sexually transmitted diseases (STDs) pose to that arise each year more than 340 million new cases of Papilloma Virus (HPV), among men and women aged 15 to 49 years or in their more sexually active age. HPV is

-

of positive samples worked, demonstrating the presence of the virus, although in lower percentage than expected. Conclusion: The detection of HPV was detected using tion of genomic material examined during the process of Keywords: HPV; Cervical Cancer; nested PCR CPE – 1881 Proliferating cell nuclear antigen (PCNA) expression in induced lesions in mice skin treated with plant extract 1.Laboratório de Biologia Celular e mutagênese. CCO/UFSJ, Brazil. 2. Laboratório de Patologia Comparada - LPC, - ICB/UFMG, Brazil 3.Laboratório de Processamento de Tecidos, CCO/UFSJ, Brazil tion and remodeling. Several treatments and products have

To evaluate cell proliferation in skin, in the process of healing, in mice treated with plant extracts. Methodology: Swiss line in which were caused cutaneous lesions. The daily treatment were applied topically and consisted of lyophlized extracts of two species of the Plantago (G1) and Siparuna (G3) in aqueous solution, one group received the treatment, and another group received only water. The daily application of the treatments were performed on top the dorsal region of each animal. After 9 days of therapy and sectioned (4µm) in microtome for mounting slides. Immunohistochemistry was performed using a proliferation cell nuclear antigen (PCNA) at a concentration of for the 4 groups: 27,18 + 2; 20,9 + 1,1; 15,4 + 1 e 12,9 + 2,3, Plantago, controle, Siparuna e pomade, respectively. Conclusion: The application of the Plantago extract does accelerate the healing process of open wounds in mice and Keywords: PCNA, healing

of HPV in women with cervical cancer in the state of

100

CPE - 1884 Morphological aspects and osteogenic differentiation

2011 5 (2)

of mesenchymal stem cells cultured on new biomaterials ros, L.A.1,2; Gondim, T.G.F.3; Bassoli, A.C.D.G.1; Silva, L.L.S.1; Silva, E.C.1; Aguiar, J.L.A.4; Medeiros, P.L.1,2,3 1. Department of Histology and Embryology, CCB - UFPE, Brazil. 2. Postgraduate Program in Therapeutic Innovation, CCB - UFPE, Brazil. 3. Postgraduate Program in Pathology, CCS - UFPE, Brazil. 4. Postgraduate Program in Surgery of Hospital das Clínicas CCS - UFPE, Brazil.

identify the Methicillin Resistant Staphylococcus Aureus (MRSA) in patients with soft-tissue infection treated at the medical primary care of the Sanitary District II in Recife -

-

-

consisting in the interview of patients who visited the sion and drainage of the infection site. Results: There were analyzed 119 patients. The most common lesion found

The medication class which were found more resistance and osteogenic differentiation process of mesenchymal -

cal procedure associated with an oral cephalexin or quinolone when necessary, is enough to treat the skin and soft

Keywords: Staphylococcus aureus, Methicillin Resistant, MRSA for 5min, and then were centrifuged and resuspended in CPE – 1885a Determination and pharmacokinetics research of

mer sugarcane gel was used to evaluate the cell growth, adhesion and spreading. Results: The new supports sustained the growth of cell colonies with elongated morphology

alho Neto, J.L.3,4; Aquino, T.M. 1; Alves, A.J.1. 1. Departamento de Ciências Farmacêuticas. Universidade Federal de Pernambuco, Brazil, 2. Faculdade Pernambucana de Saúde, Brazil, 3 Hospital Mermorial Guararapes, Brazil. 4 Maxiclínica Médica, Brazil

rounded and irregular shapes. During osteogenic differendral aspects, so suggests a secretory function; already on two weeks it was noted through SEM, a high density of cells with extensive network of projections attached to the mers of sugarcane used as new cell supports promoted the adhesion and growth of MSCs, displaying one of the essential properties for future application in tissue engineerKeywords: biopolymers; mesenchymal stem cells; osteogenic differentiation CPE – 1885 Methicillin Resistant Staphylococcus aureus in patients with skin infection Alves, AQ 1; Alves, Rocha, R.M.F. 1, Alves, R.O.R1, Martins Filho, E.D.1; ALVES, AJ2 1. Faculdade Pernambucana de Saúde, Brazil, 2. Departamento de Ciências Farmacêuticas. Universidade Federal de Pernambuco, Brazil Background: Staphylococcus aureus (staph), is a common exist and not all of them are harmful. MRSA is a strain -

(HPLC) and to compare the pharmacokinetics parameters healthy and nonsmoking volunteers were selected. The study was conducted according with the current revision of the Declaration of Helsinki. Each volunteer signed an Ethical permission was taken to approve the protocol and consent form of this study from the Ethical Review Committee of Hospital Memorial Guararapes. Blood samples were collected from each volunteer over 36 hours after 2.0, 3.0 4.0, 6.0, 8.0, 12.0, 24.0, and 36.0 h. Results: Means intervals are: 0, 1.032, 1.685, 2.026, 2.105, 1.949, 1.741, -

is one of the most common causes of skin infections and occasionally the cause of serious illness when affects the

Kurumin Linux software. Mean (Ttest) of area under the 101

Experimental Pathology and Health Sciences

-

physical exercise during development and analysis their

3.571 hr (93.58), and 0.204 and 0.207 hr-1 (91.61) for the Linux and Pharmkit programs, respectively. Conclusions:

treadmill during 15-35 days of life. It consisted in 5 weekly

When the animals reached the age of 90-120 days they parameters CPE – 1885b Validation of quantitative resorcinol method for meaA.J.1; 1. Departamento de Ciências Farmacêuticas. Universidade Federal de Pernambuco, Brazil, 2. Faculdade Pernambucana de Saúde, Brazil, 3. Departamento de Antibióticos. Universidade Federal de Pernambuco, Brazil,

Results: Body weights at 7, 14, 21, 28, 35 days of life and 13.7±2.8g, 24.4±6.6g, 36.9±9.7g, 54.6±15.9g, 75.5±21.8g and 283.6±41g, for the E group; and 14.9±3.7g, 25.7±7g, 41.8±8.3g, 62.4±13.8g, 77.3±19g and 314.5±32.2g, for the

ductive organs. The seminal vesicles produces a slightly

0.0025±0.0004g and 3.3500±0.2726cm, for E; and 0.7584±0.0825g, 0.0024±0.0002g, and 3.4857±0.3078cm trophic and serial cartilage in the epiphysis were 1.56±0.24 and 3.36±0.59 for E; and 2.13±0.56 and 3.68±0.59 for S, respectively. Conclusions: Physical exercise early in life can -

tozoa). Fructose is also secreted from the accessory sex

Keywords: Exercise; Morphometry; Femur

found in sperm plasma. The fructose occupies the most

the semen or sperm affects more than one-third of the To reduce the amount of hydrochloric acid and validation of the methodology. Methods: Quantitative measurement method. In this experiment the quantity of HCl was reagent (0,1g resorcinol and 0,1g of urea dissolved in 100ml

CEP - 1887 Behavior of hepatosplenic schistosomiasis mansoni in the municipality of Zona da Mata Sul de Pernambuco L.R.A1; Xavier, A.T1; Silva, G.R2; Domingues; A.L.C3 1 Universidade Federal de Pernambuco – Centro Acadêmico de Vitória /CAV - Brasil 2 Programa de Pós-Graduação em Patologia – CCS/UFPE – Brasil. 3 Universidade Federal de Pernambuco – Centro de Ciências da Saúde/CCB - Brasil Introduction: The situation of Schistosomiasis in Pernam-

-

disease prevalence in Brazil in recent years it still remains

546nm. Results: The standard curves were linear over the

form. This state has the highest mortality in the country. Although new cases of disease present in expansion for

racy were investigated with quality control (QC) samples

Zona da Mata is still standing out for its high endemicity. clinical aspects of carriers of the hepatosplenic schistoso-

the amount of HCl, this method is less expensive and less Keywords: Infertility, fructose, semen, Selliwanoff CPE - 1886 Body weight evolution and femoral bone features of adult rats submitted to physical exercise during development Vasconcelos, A.C.S.; Silva, T.C.O.; Melo, M.P.P.; Monteiro, H.M.C.; Amancio-Dos-Santos, A.; Maia, L.M.S.S. Universidade Federal de Pernambuco, Brazil Background: Physical exercise includes all physical activity that is programmed, structuralized and repetitive. It has

102

Cross-sectional study with 30 patients from this town at-

derwent previous treatment for portal hypertension with

2011 5 (2)

of cases representing the main co-existing infections in this casuistry. Conclusions: The study suggests the onset where to date there was no reports in the literature on the

developing of cancers, among them cancer of the cervix, mortality rate. The polymorphism of glutathione S-transferase (GST) is closely linked to an increased risk of cervi-

need for continued vigilance of the Control Program for the Schistosomiasis Eradication in the city. Keywords: schistosomiasis mansoni, epidemiology, California

study was to evaluate the risk of cervical injury in HPVpositive patients and correlate with GST polymorphism,

CPE – 1887b Schistosomiasis mansoni: An educational and methodological approach in the state education in the Zona da Mata de Pernambuco Silva, P.C.V1; Serrano, S.Q1; Xavier, A.T1; Almeida, M.I1 ; Vale, L.P.S1 1. Universidade Federal de Pernambuco – Centro Acadêmico de Vitória/CAV - Brasil

were analyzed. PCR was performed using one of the genes

an intestinal parasite Shistosoma mansoni. It represents a countries. In Brazil, the Northeast region has the highest rates of endemic disease. The control of this disease is

was used as negative control. Electrophoresis in agarose

17 samples presented cervical lesions, 48 showed the presence of genes GSTM1 and GSTT1, 30 presented GSTM1 null genotype, 12 presented T1 null genotype and 10 had

water treatment, control of intermediate hosts, pharma-

(more than three children) alone increased 3 times the risk of cervical lesions. The null GSTM1 genotype increased cohol consumption there was an increase of this risk over

actions regarding the prevention and control of Schisto-

did not act as a risk factor in any of the correlations made. Conclusions: The parity alone act as a risk factor for development of cervical injury, as well as null M1 genotype

de Santo Antão-PE. These students made up the Group 1 prior the educational activity (lecture) and Group 2 after the action at different times. Teaching material (illustrated

studied included: education level, age, clinical form of schistosomiasis, symptoms, treatment, prevention and in-

Keywords: papillomavirus; glutathione S-transferase; cervical lesions CEP – 1890 Evaluation Of Antitumor Activity Of Barbatic Acid Of Cladonia Salzmannii In Sarcoma-180 Through Histochemical Agnor Method Santos, A.F.S.*; Filho, A.F.S.; Santos, A.B.S.; Farias, W.L.R.; Falcão, E.P.S.; Júnior, F.C.A.A.; Santos,N.P.S Universidade Federal de Pernambuco – Centro Acadêmico de Vitória, PE, Brasil. -

Educational activities were evaluated on four topics: cycle, clinical presentation, treatment and disease prevention. Group 2 had median of right answers higher than group 1 in 7 topics addressed. Conclusions: The educational pro-

tant indicator of tumor conduct is the cell proliferation,

-

associated to nucleonar organized regions (NORs), present in different amounts in malignant cells and normal

disease in the city. Keywords: Health Education, Schistosomiasis mansoni, Pernambuco

technical AgNORs. Methodology: Tumor cells (5,0 x 106

CEP - 1889 Genetic polymorphism of glutathione s-transferase as a risk factor for cervical injury alone and combined with other factors in HPV-positive patients Soares, A.J.O.1, Medeiros, A.L.P.1, Bruneska, D. 1,2, Lima Filho, J.L. 1,2, Araújo,R.F.F.1,2 1. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, Recife-PE, Brasil 2. Departamento de Bioquímica –UFPE, Recife-PE, Brasil

region of mices Swiss (30-40g). 24 hours after inoculation the other (control-group) received only NaCl solution histological slides were visualized and photographed in

the control group (1,50 and 1,55 respectively), without 103

Experimental Pathology and Health Sciences

cells evaluated in animals treated as in the control group tive capacity of the same. Keywords: AGNORs, Barbatic acid, antitumor activity CEP – 1890a Effect Of N-Acetylcysteine On Schistosma Mansoni perimental Schistosomiasis Santos, A.F.S.1; Aires, A.L.1; Silva, A.G1. Filho, N.T.D.P.1; Ramos, M.L.C.1; Araújo, S.B.D.1; Costa, V.M.A.1; Souza, 1. Universidade Federal de Pernambuco, UFPE Introduction: Schistosomiasis mansoni is characterized logical lesions. N-acetylcysteine (NAC) attenuates liver

clinical signs, especially when dogs showed atypical clinical of the Immunohistochemical detection of Leishmania (Leishmania) infantum in gastro intestinal tract of dogs. Methodology: Fragments of duodenum, jejunum, ileum, cecum, colon and rectum from 36 dogs with serological

tissue sections.Results: Amastigotes forms of L.(L.) infanmucosae. Conclusions: The Immunohistochemical detection of L.(L.) infantum in duodenum, jejunum, ileum, cecum, colon and rectum of naturally infected dogs showed the parasitism of non-organ lymphatic tissue associated. Keywords: Canine Medicine, Patholohy, Canine leishmaniasis CEP – 1915

detoxifying properties. Methodology: Swiss female mice were percutaneously infected with 50 S. mansoni cerPZQ, NAC+PZQ and Control group that only received ized after 59 consecutive days. Oral administration of 100

skin wounds in dogs: histopathological and morphometric study Pereira Júnior, JR1; Menezes, FF1; Oliveira, VVG1; Silva Junior, VA1; Coelho, MCOC1 1. Federal Rural University of Pernambuco, Brazil -

ments the animals were perfused to recover worms and fragments of intestine were used to evaluate the pattern histological sections that were stained with Hematoxylin and eosin and Masson trichrome. The samples were used covered in controls and in animals treated with NAC, PZQ and NAC+PZQ was 22.3 (±2.4), 15.0 (±1.9), 0.0 and 0.0 -

and morphometric aspects of skin wounds in dogs treated ogy: Three lesions were produced in 18 dogs on the right with calendula ointment, the median wound was treated with vaseline and the caudal injury received saline soluthree times: seven, 14 and 28 days. For histopathological and for morphometry was used an integrating ocular con-

of hepatic granulomas. In groups NAC and NAC + PZQ spectively. numerical density of granulomas was reduced in groups NAC, NAC + PZQ and PZQ, respectively. Conclusion: Mice infected with S. mansoni and treated with NAC e NAC+PZQ showed reduction in parasite load, the egg killed in intestinal tissue. Keywords: Schistosomiasis, NAC, granuloma CEP - 1901 Immunohistochemical detection of Leishmania (Leishmania) infantum (Nicolle, 1908) parasites in gastro intestinal tract of dogs Ghinato, L.1; Maia, F.C.L. 2, Alves, L.C. 1; Tafuri, V.L. 3; Figueiredo, M.M. 3; Torres, S.M. 1; Pimentel, D.S. 1 1. Laboratório de Doenças Parasitárias dos Animais Domésticos. DMV/UFRPE, Brasil. 2. Laboratório de Patologia. DMV/UFRPE, Brasil. 3. Instituto de Ciências Biológicas. ICB/UFMG, Brasil. Background: Canine leishmaniasis (CanL) due to Leishmaand some of the infected animals are asymptomatic. Di104

wounds treated until the 7th day there was no reepithelialization, however, the lesions which we used calendula percentage of collagen. In the treatment of wounds up to 14 days there was no difference histopathologic and morphometric. By day 28 the experimental wounds showed cellularity in the treatment with calendula ointment. Conpathologic CPE – 1915a Leishmania sp amastigotes in the female dog reproductive system LC1; Silva Junior, VA1 1. Universidade Federal Rural de Pernambuco, Brazil Background: Dogs are the most important reservoir of

2011 5 (2)

are the primary way of transmission. Although after the infection the parasite multiply and migrate from lymphoid tissue to other organs included spleen, liver, and tives: Immunohistochemical detection of Leishmania sp amastigotes in the female dog reproductive system natuadult female dogs were referred to the outpatient clinic Brazil) and diagnosed parasitologically positive for visceral Fragments of the reproductive tract (vulva, vagina, cervix, histopathology and immunohistochemistry diagnostic. All were mounted on slides and stained with hematoxylin and eosin (HE) or further processed for immunohistochem-

junctiva are presented in dogs with natural infection of Leishmania infantum. Keywords: Leishmania sp; Conjunctiva; Immunohistochemistry CEP – 1918 Histopathological aspect and indication for surgery in penile Squamous Cell Carcinoma in horse - Case Report Silva, L. T. R1; Maia, F. C. L1; Vaz, B. B. D1. 1. Universidade Federal Rural de Pernambuco- Dois Irmãos- Brasil. Background: In the external genitalia cancer of horses, squamous cell carcinoma is prevalent in second place among skin tumors (VAN DEN TOP, 2008). The microgrowth of squamous cells invading normal tissue. The keratin of the stratum corneum which is produced in the form concentric laminated structures, known as keratin -

structural change was a mild to moderate vulvar dermatipredominantly in the papillary layer of dermis. One of dominantly composed of macrophages, plasma cells, and many lymphocytes mainly in the connective tissue layer of

equine penile squamous cell carcinoma and its malignancy and differentiation. Methodology: An equine Mangalarga senting in the penis, a hyperplastic tissue covering every aspect of the glans. There was also a permanent serous moisture look at the site, as well as areas with myiasis. The animal underwent partial amputation of the penis accord(1985), and then histopathological examination was per-

vaginal lesions related with Leishmania infantum infection. Keywords: Leishmania sp; Female dog; Immunohistochemistry

mainly composed of polymorphonuclear cells and macrophages, non reactive. In other areas, there was intense ac-

CEP – 1915b Immunohistochemical patterns of the conjunctiva of dogs with natural infection of Leishmania infantum Oliveira, VVG1; Guerra, NR1; Santos, EMS1; Severo, MS2; Faustino, MAG1; Alves, LC1 1. Universidade Federal Rural de Pernambuco, Brazil 2. Universidade Federal de Sergipe, Brazil Background: Canine Visceral Leishmaniasis (CanL) is a

There is proliferation of squamous epithelium, consisting mainly of squamous cells, which often form pearls corneas, the cells form small or large clusters, usually nodular. There is intense cellular and nuclear pleomorphism. Mitot-

megaly and lymphadenopathy.and ophthalmopathies. histological and immunohistochemical patterns of the conjunctiva of dogs with natural infection of Leishmania infantum. Methodology: Five dogs positive to L.infantum. according to serologic test (ELISA) presenting ocular alFragments of the conjunctives were collected and all tiswere mounted on slides and stained with hematoxylin and eosin (HE) or further processed for immunohistochemistry. Results: The main structural changes was a mild to ing from a focal or multifocal to diffuse process adjacent to the epithelium. Lymphoid nodules presented enlarged, however, amastigotes forms of Leishmania sp were not visualized. Amastigotes forms of Leishmania sp, in turn,

The stroma is now thick, now it is thin, however, is always well vascularized. Lymphocytes, macrophages and some sions: Although the malignancy of this tumor, partial surgical removal of the penis provided the return to normal activities a few months later. Keywords: Carcinoma, penis, horse CEP – 1918a Feline and Canine Nasal Tumors: Retrospective Cytopathological Analysis in animals attended in veterinary hospital at Federal Rural University of Pernambuco, Brazil. Silva, L.T.R1; Barros, R.B.1; Pereira, M.F1; Oliveira, A.A.F.1 1. Veterinary Medicine Department, Federal Rural University of Pernambuco (UFRPE), Brazil common tumors reported in dogs are adenocarcinoma, squamous cell carcinoma and chondrosarcoma. In the cat, nasal tumors are less commonly diagnosed, correspondincluded. In this species, squamous cells carcinoma and lymphoma are the most frequent tumors. Among those 105

Experimental Pathology and Health Sciences

ing the circulation of these isolates in the three units of Analyze the frequency of nasal tumors in dogs and cats pathology service of the department of veterinary medi-

gene. Conclusions: These results indicate a high polymorphism, high rate of resistance to methicillin and frequency

the animals. Methodology: Cytopathological diagnosis of pathology area, in the veterinary medicine department of perform this, records of the cytologic examination were discarded. The data was transferred to spreadsheets consis, and were compared. Descriptive analysis was applied Results: Of the 14 diagnoses of nasal tumor analyzed, 10

Keywords: Staphylococcus aureus; Exotoxins; Methicillin CPE-1369 Prevalence of pressure ulcersand its related risk factors in patients in the unit of health of the municipality if Carpina-PE Frota, M.T.N.C1; Oliveira, F.H.S2; Santos, E. R. R dos2; Mello L.A.2, 3 2.Maurício de Nassau Faculty, Brazil. 3.Dentistry Faculty of Recife, Brazil.

frequently found tumors independently of the species challenge for the health teams, demonstrating the importance of continuous updating of knowledge in this area -

the prevalence of pressure ulcers in patients admitted to

Conclusions: The squamous cell carcinoma are the most

servational, descriptive, transversal and quantitative clinical inspection through the skin, and monitor patients through

and cats. Keywords: Pets; Intranasal tumors; cytology CPE – 1926 Molecular characterization of Staphylococcus aureus isolated from clinical specimens in Brazil

with a minimum stay of 24 hours of age or more than 18 none of the patients developed pressure ulcers within the

T.C. 3 1. Federal University of Pernambuco – UFPE, Brazil 2. Department of Genetics and Molecular Biology – PPGG/ Federal University of Pernambuco – UFPE, Brazil 3. Department of Microbiology- Oswaldo Cruz Foundation, Research Center Aggeu Magalhães - CpqAM/Fiocruz, Brazil Background: Staphylococcus aureus is a major pathogen associated with serious nosocomial diseases. Several types staphylococcal exfoliative toxins (ETs) and toxic shock syndrome toxin 1 (TSST-1). Around the world, including methicillin-resistant S. aureus (MRSA) and therapeutic op-

site most frequently affected the sacral region. There was a certain lack of unity in the essential materials for the relation to the prevention and treatment of ulcers. Keywords: Pressure ulcers, Risk Factors, Care of Nursing CPE-1872 Association of human papillomavirus genotypes with p16INK4A protein expression and intraepithelial lesions in women in the state of Pernambuco - Brazil

a molecular characterization of S. aureus isolated from Brazil. Methodology: A total of 40 clinical isolates were analyzed from different sources of infection and hospital departments. The Polymerase Chain Reaction (PCR) was used for the detection of the genes of exotoxins and the

A.1, Silva-Neto, Jc.1. 1 - Clinical Cytology Laboratory of the Federal University of Pernambuco - Brazil. Introduction: Approximately 40 genotypes of human papillomavirus (HPV) affect the genital tract, 15 of these are associated with high oncogenic risk for developing cervical

The investigation of polymorphism of the coagulase gene of the isolates. Results: All isolates showed the gene seg,

lease of cellular transcription factor E2F. The E2F acts in the stimulation of cell replication and expression of the tein p16INK4A. traepithelial and invasive HPV genotyping and associate p16INK4A protein expression in women from the interior

Material and methods: A retrospective cross-sectional 106

2011 5 (2)

and histology (85 lesions LSIL, 89 HSIL, 37 invasive squamous cell carcinoma), immunohistochemical detection of p16INK4A protein and PCR-RFLP for genotyping of the ture II HPV negative. Results: Of the 211 cases studied the presence of

carbamate Marques P1,3, Jorge M1,3, Cavalcanti K2, Farinha R3 1. Faculdade de Farmácia da Universidade de Coimbra 2. Universidade Federal de Pernambuco 3. Faculdade de Medicina da Universidade de Coimbra, Serviço de Patologia Experimental cide used in crops, trees and ornamental plants. The intake Twenty Wistar mail rats, 8 weeks-old were randomly dis-

cell carcinoma were positive for p16INK4A prevalence of complete necropsy was performed.

for high-risk HPV showed an association with high-grade cervical lesions and invasive. Keywords: HPV, p16, genotyping CPE-1470 Morphometric Analysis of the Intestinal Mucosa in Experimental Alcoholism Marques P1,3, Jorge M1,3, Farinha R3, Cavalcanti K2 S3 1. Faculdade de Farmácia da Universidade de Coimbra 2. Universidade Federal de Pernambuco 3. Faculdade de Medicina da Universidade de Coimbra, Serviço de Patologia Experimental The impact of alcohol consumption is well known for mechanisms involved in the alcohol consumption is sus2,5 million deaths per year1. Twenty Wistar mail rats, 8 weeks-old were randomly disistration of wine incorporated in the diet in the dose of thetics and the complete necropsy was performed.

evaluate the intestinal villi height (H). The results of the measurements were divided in 5 groups:

evaluate the intestinal villi height (H). The results of the measurements were divided in 5 groups:

the control group. medium intestinal villi and high intestinal villi decreased These results suggest that the administration of high doses portant morphologic changes in the mucosa of small intestine, which may interfere with the physiology of this organ. CPE-1553 Evaluation of chronic treatment with 5-phosphodiesterase inhibitors on the prostate of mice Gomes, F.O.S.1; Carvalho, M.C.1; Silva, B.S.1; Rocha, Rodrigues, G.B1; Oliveira, A.G.V. 2; Peixoto, C.A1,2 1. Centro de Pesquisas Aggeu Magalhães- CpqAM/FIOCRUZ, Brazil 2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/ MCT, Brazil phodiesterase-5 (PDE-5) used extensively for the treatment of erectile dysfunction. Recent studies have shown -

group. Comparing with the control group, in Group II was veri-

-

medium intestinal villi and high intestinal villi decreased -

the treatment regimen, animals are euthanized in CO2 Results: Prostate sections from control group presented -

CPE-1470a Morphometric Analysis of the Intestinal Mucosa in Experimental Intoxication With 1-naphthyl methyl107

Experimental Pathology and Health Sciences

papillary invaginations, as well as changes in the epithelium and increase of secretion in lumen. ImmunohistochemiPKA, PKG and sGC immunopositivity. This same result

cation staining analyses were performed using the software Graph Pad Prism v.05. Conclusions: According to our remolecular mechanisms of the mice prostate. CPE-1562 LPSF/GQ-02 reduces the expression of matrix metalloproteinase-9 (MMP-9) and evolution of atheroSilva, A.K.S.1; Torres, D.O.C.1; Rocha, S.W.S.1; Silva, B.S.1; Oliveira, F.G.1; Nunes, A.K.S.1; Lima, M. C. A.3; Galdino, S. L. 3; Pitta, I. R.3; Peixoto, C.A1,2 1. Centro de Pesquisas Aggeu Magalhães- CpqAM/FIOCRUZ, Brazil 2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/ MCT, Brazil 3. Laboratório de planejamento e síntese de fármacos, UFPE, Brazil Background: Experimental evidence suggests that matrix metalloproteinase-9 (MMP-9) may play a causal role in new-onset cardiovascular disease. Changes in the expres-

Keywords: matrix metalloproteinase-9, atherosclerosis, new drugs. CPE-1568 in the central nervous system, in a multiple sclerosis model Nunes AKS1,2, Luna R2,Oliveira AGV1, Peixoto CA1,2, Rapôso C1 1. Laboratório de Microscopia, Ministério de Ciência e Tecnologia, Centro de Tecnologias Estratégicas do Nordeste (MCT/CETENE) 2. Laboratório de Ultraestrutura, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brazil. tive effects in neurodegenerative diseases. Activation of microglial cells may trigger the overproduction of sevjective: This study investigated the effects of chronic treata multiple sclerosis (demyelination) model. Methodology:

Controls received pure chow and water. After transcardiac -

in the pathogenesis of several cardiovascular conditions such as atherosclerosis, resulting in atherosclerotic plaque

-

16 on the expression of MMP-9 and evolution of atherodivided into four groups: 1-fed with high-fat diet (HFD)

-

ments were conducted for 10 weeks and in the last two animals, comparing with control, indicating microglial the aorta was quickly dissected and processed for optical droplets inside, which characterize the foam cells. In the the tunic media. Morphometric analysis demonstrated that the treatment with pioglitazone did not reduce the area of the atherosclerotic lesions when compared with the HFD

groups. Analysis of expression of MMP-9 showed that the expression of MMP-9 compared with the HFD. Inexpression of MMP-9 compared with the HFD group. promising candidate for the treatment of atherosclerosis. 108

pared to cuprizone group, indicating normal microglial activation. Conclusions: The increased levels of cGMP, modulator, regulating cytokines levels and protecting mineurodegenerative diseases. CPE-1578 tion, necrosis and hemorrhage in carbon tetrachloride (CCl4)-induced liver injury in mice Rocha, S.W.S.1; França, M.E.R1; Silva, J.C.2; Silva, B.S.1; G.B.1; Oliveira, A.G.V. 2; Peixoto, C.A1,2 1. Centro de Pesquisas Aggeu Magalhães- CpqAM/FIOCRUZ, Brazil 2. Centro de Tecnologias Estratégicas do Nordeste- CETENE/ MCT, Brazil

2011 5 (2)

Background: Pharmacological studies showed that Diethof DEC on the CCl4-induced hepatotoxicity in Swiss mice.

the animals were euthanized 24h later. Liver fragments were processed for light microscopy and immunohistochemical assays. Results: Liver sections from control group presented normal liver architecture, showed well preserved tissue, composed of radially arranged cords of hepatoof the CCl4 group showed large areas of extensive necrose, mainly pericentral, loss of hepatic architecture, lipid ministration completely prevented liver necrosis reduced the DEC+CCL4 group showed minimal hepatic damage. Immunohistochemical analyses of the control group other hand, strong TNF-expression was found in the CCl4 in pericentral areas. No apparent TNF-expression was detected in nonparenchymal cells. DEC+CCl4 group furstaining analyses were performed using the image program Gimp 2.6 software. Conclusions: According to present re-

of DEC on the molecular mechanisms involved in the inKeywords: Diethylcarbamazine, hepatocytes, anti-

109

Experimental Pathology and Health Sciences

110

2011 5 (2)

I LUSO-BRAZILIAN CONGRESS OF THE EXPERIMENTAL PATHOLOGY

XI International Symposium on Experimental Techniques Federal University of Pernambuco

Selected Oral Presentacions

Oral Presentacions (O1) Day 11/11/11 - 11.00 am -12.00 pm

Oral Presentacions (02) Day 12/11/11 - 14 pm – 15.20 pm

CPE-1465a Murine Experimental Model Of Chagasic Megacolon França Campos,C.; Cassin Duz, A.L.; Dantas Cangussu, S; Veloso, V.M.2; Bahia, M.T.; Silva, T.A.; Arantes, R.M. E.

CPE-1448 Evaluation of the mutagenic potential of essential oil from leaves of Rollinia leptopetala RE Fries Mangueira, V.M.; Brito, M.T.; Batista, T.M.; Moreira, M.M.B.; Pessoa, D.R.; Viana, W.P.; Tavares, J.F.; Pessoa, H.L.; Diniz, M.F.F.M.; Castello Branco, M.V.S.

CPE-1457 Gastroprotective Effect Of Cissampelos Sympodialis Eichl. (Menispermaceae): Role Of Sulphydryl Compounds And Nitric Oxide Sales, I.R.P.; Lima, G.R.M.; Caldas-Filho, M.R.D.; Falcão, H.S.; Barbosa-Filho, J.M.; Batista, L.M. CPE-1445 Essential oil from leaves of Lippia microphylla Cham.: antitumor effect and histophatological study Batista, T.M.; Moura, A.P.G.; Lunguinho, D.M.; Brito, M.T.; Xavier, A.L.; Pita, J.C.L.R.; Silva, T.G.; Tavares, J.F.; Diniz, M.F.F.M.; Castello-Branco, M.V.S. CPE-1399 Mutagenic potential evaluation of hydroalcoholic extract of Zornia brasiliensis (Fabaceae) Moreira, M.M.B.; Batista, T.M.; Brito, M.T.; Mangueira, V.M.; Rolim, T.L.; Tavares, J.F.; Silva, M.S.; Pessoa, H.L.; Diniz, M.F.F.M.; Castello Branco, M.V.S. CPE-1392 Salivary Glands in Experimental Intoxication with DMBA Nery da Silva, E., Alves da Silva, M.A., Araújo Félix Junior, J., Pereira, F., Farinha Henrique Marques, R.H., Silvério Cabrita, A.M., Paiva Figueiredo, A.S. , Carvalho Freitas Ramos, T.

CPE-1446a In vitro and in vivo antitumor activity of es(Annonaceae) Sousa, T.K.G.; Macêdo, B.V.F.L.; Lunguinho, D.M.; Brito, M.T.; Moura, A.P.G.; Pita, J.C.L.R.; Xavier, A.L.; CPE-1439a Chemical composition and antitumor activity of essential oils of species from Northeast of Brazil Pessoa, D.R.; Xavier, A.L.; Lunguinho, D.M.; Moura, A.P.G.; Brito, M.T.; Costa, V.C.O.; Pita, J.C.L.R.; Tavares, J.F.; Diniz, M. F. F. M.; Castello-Branco, M.V.S. CPE-1436 An alternative method to induce experimental pulmonary infection by Paracoccidioides brasiliensis in mice Ribeiro, RSA; Cardoso, RA; Teixeira, GAPB; Dias, EP; Figueiredo Júnior, I; Verícimo, MA CPE-1728 Histomorphometric Analysis Of Skin Wound Healing Undergoing Acupuncture Therapy: Study In Rats Souza, M.F.A.M.; Silva, E.L.V.; Santos, J.A.A.; Freire, F.J.A.;Pinto, R.H.; Aguiar Jr, F.C.A.

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XI International Symposium on Experimental Techniques XI

2011 5 (2) I LUSO-BRAZILIAN CONGRESS OF THE EXPERIMENTAL PATHOLOGY XI International Symposium on Experimental Techniques Federal University of Perna...

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